• 한국환경성돌연변이발암원학회지
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• 제23권1호
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• pp.30-34
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• 2003

Recent industrial society has human widely exposed to PAHs (polynuclear aromatic hydrocarbons) that are comming from the incomplete combustion of organic material as wider spread environmental contaminants. Biological activities of PAHs are not known although PAHs are considered as carcinogens. Our laboratory have been studied the effect of PAHs in the mouse liver hepa 1 cells. In this study, we examined the mouse liver hepa-l cells as a new bioassay system to evaluate bioactivity of PAHs. We have selected 13 PAHs to examine bioassay using cyp1a1-luciferase reporter gene expression system where cyp1a1 1.6 Kb 5flanking region DNA was cloned in front of luciferase reporter gene and this plasmid was transfected into hepa 1 cells transiently. This cells then used for the study to observe the effect of PAHs. We demonstrated that PAHs induced the CYP1A1 promoter and 7-ethoxyresolufin O-deethylase (EROD) activities in a concentration-dependant manner. Some of PAHs showed stronger stimulatory effect on CYP1 gene expression than TCDD. Acenaphthene, anthracene, fluorine, naphthalene, pyrene, phenanthrene, carbazole were weak responders to cyp1a1 promoter activity stimulation and EROD induction in hepa 1 cells and these chemicals seemed to respond less to EROD than cyp1a1 promoter activity. Benz(a)anthracene, benzo(b)fluoranthene, benzo(k)fluoranthene, chrysene, and dibenzo(a,h)anthracene showed strong response to cyp1a1 promoter activity stimulation and also EROD induction in hepa 1cells. Results of dose response study suggested that four strong responding PAHs, such as benzo(a)anthracene benzo(k)fluoranthene, chrysene, and dibenzo(a, h)anthracene might be mediated through arylhydrocarbon receptor system in hepa1 cells.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• 제2권1호
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• pp.57-68
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• 1998

Polycyclic aromatic hydrocarbons (PAMs) are ubiquitous contaminants in marine environments. PAMs enter estuarine and nearshore marine environment via several routes such as combustion of fossil fuels, domestic and industrial effluents and oil spills. PAHs have been the focus of numerous studies in the world because they are potentially carcinogenic, mutagenic, and teratogenic to aquatic organisms and humans from consuming contaminated food. However, one can hardly find any available data on PAM content in marine organisms in Korea. The present study was carried out in order to determine PAM content in oysters from the intertidal and subtidal zones of Chinhae Bay, which is located in near urban communities and an industrial complex, and the bay is considered to be a major repositories of PAHs. 16 PAHs were analyzed by High Performance Liquid Chromatography (HPLC) with uv/vis and fluorescence detectors in oysters: they are naphthalene (NPTHL), acenaphthylene (ANCPL), acenaphthylene (ACNPN), fluorene (FLURN), phenanthrene (PHEN), anthracene (ANTHR), fluoranthene (FLRTH), pyrene (PYR), benzo(a)anthracene (BaA), chrysene (CHRY), benzo(b)fluoranthene (BbF), benzo(k)fluoranthene (BkF), benzo(a)pyrene (BaP), dibenz(a,h)anthracene(DahA), benzo(g,h,i)peryne (BghiP) and indeno(1,2,3,-cd)pyrene (I123cdP). The PAM contents in oysters from the intertidal and subtidal zones of Chinhae Bay ranged from < 0.1 to 992.0 ug/kg (mean $69.8\pm9.8$ ug/kg).

• 생태와환경
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• 제41권3호
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• pp.331-337
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• 2008

Polycyclic aromatic hydrocarbons (PAHs) derived from leakage of fossil fuels and incomplete combustion of organic materials have been considered as harmful contaminants in environments. This study evaluated the effect of benzo[k]fluoranthene (BkF), one of the PAHs, using the multiple biomarkers and applied the integration model with those biomarker responses. After 10 days of the exposure at the measured concentrations of BkF (6, 25, and 45 ${\mu}g\;L^{-1}$), the changes of the four biomarkers, that is, 7-ethoxyresorufin-O-deethylase (EROD), DNA single-strand breaks (Comet), acetylcholinesterase (AChE) and vitellogenin (VTG) in the common carp (Cyprinus carpio) were observed. The standardized values of four biomarker responses were computed and integrated as star plots, representing Integrated Biomarker Respnse (IBR) values. DNA damage was induced in a dose-dependent manner, and increased significantly compared with that in the control. EROD and VTG levels were significantly elevated at low concentrations of BkF. On the other hand, AChE activities were not altered by BkF. IBR values increased as the exposure concentrations increased. Thus, the metabolic, endocrine and genetic changes of the biomarker responses in the common carp exposed to BkF should be considered in the case of the ecological risk assessment of the BkF in fish and it can be used as a biomonitoring tool in aquatic ecosystems. In addition, star plots can be used as a useful analysis tool in multibiomarker integration approach.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• 제10권S_3호
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• pp.113-119
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• 2001

Surface sediments(0~5 cm) were sampled from 22 stations in Ulsan Bay, one of the most highly industrialized regions in Korea, in November 2000. The sediment samples were analyzed for their polycyclic aromatic hydrocarbon(PAH) content using a gas chromatography coupled to a mass spectrometer detector(GC/MSD). The total PAH concentrations in the sediments varied from 14 to 7108 ng/g dry weight with a mean value of 1052 ng/g dry weight. The level of carcinogenic PAHs ranged from 6 to 2396 ng/g dry weight with a mean value of 433 ng/g dry weight. The highest PAH concentrations in the sediments from Ulsan Bay were found at Station U8, whereas the lowest levels were observed at Stations U2 and U 17. The PAH distribution exhibited a decreasing gradient from the inner basin to the outer bay. The predominant contributors to the aromatic ring groups of the 16 PAHs were four- and five-ring groups, such as fluoranthene, pyrene, benzo[a]anthracene, chrysene, benzor[b]fluoranthene, benzor[k]fluoranthene and benzo[a]pyrene, while two- and three-ring aromatics, like naphthalene, acenapthylene, acenaphthene, fluorene, phenanthrene and anthracene, only exhibited a low concentration. The molecular indices for phenanthrene/anthracene and fluoranthen/pyrene were used to Identify the origin of the PAH contamination in the sediments. The results indicated that the PAH contamination in Ulsan Bay was mostly Pyrolytic in origin with a Petrogenic input adjacent to Ulsan and Jangsuengpo harbor.

• 한국환경과학회지
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• 제2권1호
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• pp.57-68
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• 1993

Polycyclic aromatic hydrocarbons (PAMs) are ubiquitous contaminants in marine environments. PAHs enter estuarine and nearshore marine environment via several routes such as combustion of fossil fuels, domestic and industrial effluents and oil spills PAHs have been the focus of numerous studies in the world because they owe potentially carcinogenic, mutagenic, and teratogenic to aquatic organisms and humans from consuming contaminated food. However, one can hardly find any available data on PAM content in marine organisms in Korea. The present study was carried out in order to determine PAH content in oysters from the intertidal and subtidal zones of Chinhae Bay, which is located in near urban communities and an industrial complex, and the bay is considered to be a major repositories of PAHs. 16 PAHs were analyzed by High Performance Liquid Chromatography (HPLC) with uv/vis and fluorescence detectors in oysters: they are naphthalene (NPTHL), acenaphthylene (ANCPL), acenaphthene (ACNPN), fluorene (FLURN), phenanthrene (PKEN), anthracene (ANTHR), fluoranthene (FLRTH), pyrene (PYRf), benzo(a)anthracene (BaA), chrysene (CHRY), benzo(b)- fluoranthene (BbF), benzo(k)fluoranthene (BkF), benzo(a)pyrene (BaP), dibenz(a, h)anthracene (DhA), benzo(g, h, i)peryne (Bghip) and indeno(1, 2, 3, -cd)pyrene (I123cdP). The PAH contents in oysters from the intertidal and subtidal zones of Chinhae Bay ranged from < 0.1 to 992.0 $\mu\textrm{g}$/kg (mean 69.8 $\pm$ 9.8 $\mu\textrm{g}$/kg). Key words . polycyclic aromatic hydrocarbon, high performance liquid chromatography, oyster, Chinhae Bay.

• 분석과학
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• 제27권5호
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• pp.254-260
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• 2014

본 연구에서는 열처리 공정에서 발생하는 폐기물 중 무기물질류 15 종과, 유기물질류 24 종 (PCDD/PCDFs, PAHs)에 대한 배출 특성을 파악하고자 수행하였다. 납 열적 야금에서 발생하는 폐기물 (EWC 10 04)에서 규제 무기금속물질류 중 Pb은 가장 높은 농도를 보였다. 이는 EWC 10 04에서 분진으로 인한 결과로 판단된다. 아연 열적 야금에서 발생하는 폐기물(EWC 10 05)에서 규제 무기금속물질류 중 Zn이 높은 농도로 나타나 EWC 10 05에서 분진으로 인한 결과로 판단되며, 또한 구리 열적 야금에서 발생하는 폐기물(EWC 10 06)에서 Cu가 65,177 mg/kg으로 규제기준 (100 mg/kg)보다 651 배 높게 나타난 것은 EWC 10 06에서 분진으로 인한 결과로 판단된다. 분진은 다이옥신 0.0005~11.748 ng-TEQ/g으로 검출되었고, 소각재는 0.0027 ng-TEQ/g로 나타났다. 다이옥신의 규제기준을 초과하는 시료는 없는 것으로 조사되었다. PAHs의 함량은 Naphthalene는 ND~118.9 mg/kg, Phenanthrene는 ND~9.6 mg/kg, Benzo[b]fluoranthene은 ND~48.4 mg/kg, Benzo[a]pyrene는 ND~62.6 mg/kg, Fluoranthene는 ND~10.7 mg/kg, Benzo[a]anthracene는 ND~11.5 mg/kg의 범위로 나타났다.

• 한국환경성돌연변이발암원학회지
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• 제24권4호
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• pp.198-205
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• 2004

Cytochrome P4501B1(CYP1B1) is known to be inducible by xenobiotic compounds such as policyclic aromatic hydrocarbon(PAH) and dioxins such as 2,3,7,8-tetrachloro-dibenzo-p-dioxin(TCDD). And these induction of CYP1B1 is also regulated by many categories of chemicals. In order to investigate the effects of several chemicals on CYP1B1 gene expression in Hepa-I and MCF-7 cells, 5' flanking DNA of human CYP1B1 was cloned into pGL3 basic vector containing luciferase gene, and then transfected into these cells. After treatment of chemicals, the luciferase activity was measured. CYP1B1 enzyme metabolize PAHs and estradiol. CYP1B1 metabolize estradiol to 4-hydrozyestradiol that is considered as carcinogenic metabolite. Recent industrialized industrialized society, human has been widely been exposed to widespread environmental contaminants such as PAHs(polycyclic aromatic hydrocarbon) that are originated from the imcomplete combustion of hydrocarbons. PAHs are known to be ligands of the AhR(aryl hydrocarbon receptor). Induction of cytochrome P4501B1(CYP1B1) in cell culture is widely used as a biomarker for PAHs. Therefore we have studied the effect of PAHs in the human breast cancer cells MCF-7 to evaluate bioactivity of PAHs. We have used the United State of America EPA selected 13 different PAHs, PAHs mixtures and extracts from environmental samples to evaluate the bioassay system. We examined effects of PAHs on the CYP1B1-luciferase reporter gene and CYP1B1 mRNA level. Benzo(k)fluoranthene and dibenzo(a, h)anthracene showed strong response to CYP1B1 promoter activity stimulation, and also CYP1B1 mRNAs increase in MCF-7 cells in a concentration-dependent manner. RT-PCR analysis indicated that PAHs significantly up-regulate the level of CYP1B1 mRNA. Some flavonoids such as genistein, daidzein, chrysin, naringenin and morin were also investigeted. These flavonoids decreased B(k)F infuced luciferase activity at low concentration. But, these flavonoids exhibited stimulatory effect at high concentration.

• 한국식품과학회지
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• 제37권4호
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• pp.537-541
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• 2005

곡류, 서류, 두류 및 그 가공품 총 200건을 대상으로 benzo(a)pyrene등 8개 PAHs 화합물에 대하여 분석하였다. 시료 중 PAHs 화합물을 n-hexane으로 추출한 후 Sep-Pak Florisil cartridge로 정제한 후 HPLC-fluorescence로 분석하였다. 분석한 대상식품 중 PAHs 평균 함량은 각각 benzo(a)anthracene $0.13{\mu}g/kg$, chrysene $0.08{\mu}g/kg$, benzo(b)fluoranthene $0.06{\mu}g/kg$, benzo(k)fluoranthene $0.03{\mu}g/kg$, benzo(a)pyrene $0.08{\mu}g/kg$, dibenzo(a,h)anthracene $0.15{\mu}g/kg$, benzo(g,h,i)perylene $0.45{\mu}g/kg$, indeno(1,2,3-c,d)pyrene $0.14{\mu}g/kg$이었다.

• 한국환경성돌연변이발암원학회:학술대회논문집
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• 한국환경성돌연변이발암원학회 2004년도 KSOT/KEMS Fall Annual Meeting
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• pp.144-144
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• 2004

• 한국환경성돌연변이발암원학회:학술대회논문집
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• 한국환경성돌연변이발암원학회 2004년도 KSOT/KEMS Fall Annual Meeting
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• pp.145-145
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• 2004