• Title/Summary/Keyword: Fluidic technique

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Characterization of Dynamic Behavior of C. elegans in Different Physical Environments (PIV 및 TFM 측정 기법을 이용한 예쁜꼬마선충의 동적 패턴 가시화 연구)

  • Park, Jin-Sung;Yun, Byoung Hwan;Shin, Jennifer H.
    • Journal of the Korean Society of Visualization
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    • v.12 no.2
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    • pp.18-22
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    • 2014
  • Caenorhabditis elegans (C. elegans) is an undulatory nematode which exhibits two distinct locomotion types of swimming and crawling. Although in its natural habitat C. elegans lives in a non-Newtonian fluidic environment, our current understanding has been limited to the behavior of C. elegans in a simple Newtonian fluid. Here, we present some experimental results on the penetrating behavior of C. elegans at the interface from liquid to solid environment. Once C. elegans, which otherwise swims freely in a liquid, makes a contact to the solid gel boundary, it begins to penetrate vertically to the surface by changing its stroke motion characterized by a stiffer body shape and a slow stroke frequency. The particle image velocimetry (PIV) analysis reveals the flow streamlines produced by the stroke of worm. For the worm that crawls on a solid surface, we utilize a technique of traction force microscopy (TFM) to find that the crawling nematode forms localized force islands along the body where makes direct contacts to the gel surface.

Nanochannels for Manipulation of DNA Molecule using Various Fabrication Molecule

  • Hwang, M.T.;Cho, Y.H.;Lee, S.W.;Takama, N.;Fujii, T.;Kim, B.J.
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.7 no.4
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    • pp.254-259
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    • 2007
  • In this report, several fabrication techniques for the formation of various nanochannels (with $SiO_2$, Si, or Quartz) are introduced. Moreover, simple fabrication technique for generating $SiO_2$ nanochannels without nanolithography is presented. By using different nanochannels, the degree of stretching DNA molecule will be evaluated. Finally, we introduce a nanometer scale fluidic channel with electrodes on the sidewall of it, to detect and analyze single DNA molecule. The cross sectional shape of the nanotrench is V-groove, which was implemented by thermal oxidation. Electrodes were deposited through both sidewalls of nanotrench and the sealing of channel was done by covering thin poly-dimethiysiloxane (PDMS) polymer sheet.

The Contact and Parallel Analysis of Smoothed Particle Hydrodynamics (SPH) Using Polyhedral Domain Decomposition (다면체영역분할을 이용한 SPH의 충돌 및 병렬해석)

  • Moonho Tak
    • Journal of the Korean GEO-environmental Society
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    • v.25 no.4
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    • pp.21-28
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    • 2024
  • In this study, a polyhedral domain decomposition method for Smoothed Particle Hydrodynamics (SPH) analysis is introduced. SPH which is one of meshless methods is a numerical analysis method for fluid flow simulation. It can be useful for analyzing fluidic soil or fluid-structure interaction problems. SPH is a particle-based method, where increased particle count generally improves accuracy but diminishes numerical efficiency. To enhance numerical efficiency, parallel processing algorithms are commonly employed with the Cartesian coordinate-based domain decomposition method. However, for parallel analysis of complex geometric shapes or fluidic problems under dynamic boundary conditions, the Cartesian coordinate-based domain decomposition method may not be suitable. The introduced polyhedral domain decomposition technique offers advantages in enhancing parallel efficiency in such problems. It allows partitioning into various forms of 3D polyhedral elements to better fit the problem. Physical properties of SPH particles are calculated using information from neighboring particles within the smoothing length. Methods for sharing particle information physically separable at partitioning and sharing information at cross-points where parallel efficiency might diminish are presented. Through numerical analysis examples, the proposed method's parallel efficiency approached 95% for up to 12 cores. However, as the number of cores is increased, parallel efficiency is decreased due to increased information sharing among cores.

A Colorimetric Glucose Assay via Concentration Gradient Paper Chip (종이기반 농도 구배 형성 칩을 통한 포도당 발색 반응 검사)

  • Kim, Taehoon H.;Shin, Hyun Young;Lee, Yun-Il;Tae, Ki-Sik;Kim, Minseok S.
    • Journal of Biomedical Engineering Research
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    • v.38 no.6
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    • pp.302-307
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    • 2017
  • This paper presents a paper-based concentration gradient chip to analyze colorimetric glucose assay. The paper-based concentration gradient chip was fabricated through a wax patterning technique that can design the fluidic channel by selectively printing hydrophobic and hydrophilic areas. Afterwards, glucose and dilution solutions were loaded into the inlet of a concentration gradient chip and each solution was then mixed sequentially at mixing channel. Finally, concentration gradient was formed at each outlet of the chip. To measure the glucose concentration of the solution in outlets, we conducted colorimetric glucose assay with fixed concentration of glucose solution (0, 5, 10, 15 and 20 mM) and obtained normalized intensity. Subsequently, glucose concentrations of the outlets were calculated by substituting the normalized intensity to linear regression function based on the normalized intensity of fixed glucose concentration. Finally, the concentration gradient of glucose was formed on the chip with the result of colorimetric assay. The concentration gradient paper chip has the potential to accurately analyze unknown glucose concentration.

Development of the High-Accuracy Multi-Component Balance for Fluidic Thrust Vectoring Nozzle of UAV (UAV용 유체역학적 추력편향 노즐의 고 정확도 다분력 시험장치 개발)

  • Song, Myung-Jun;Chang, Hong-Been;Cho, Yong-Ho;Lee, Yeol
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.41 no.2
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    • pp.142-149
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    • 2013
  • The thrust vector control technique is essential for high maneuverability of unmanned aerial vehicles. In the present study, a multi-component balance was developed to quantitatively evaluate the thrust-vectoring performance of a supersonic rectangular nozzle based on the Coanda coflowing effect. Precise calibration and detailed data analysis were performed during the development. It was found that the cross-talk errors between load cells in the balance were less than 5%, and that the unwanted errors due to high-pressure supply tubes were almost negligible, which contributed to the high accuracy of the present balance design. Some preliminary test results of the thrust-vectoring performance of the present nozzle design were also obtained and analyzed.

In-situ Patterning of Magnetic Particles in Microfluidic Channels by Forward/Reverse Local Magnet Arrangement (국소 자기장의 순/역 배열을 이용한 미세유체 채널 내에서의 강자성 입자 패턴 형성)

  • Park, Hyoun-Hyang;Lee, Ji Hae;Yoo, Yeong Eun;Kim, Jung-Yup;Chang, Sunghwan
    • Transactions of the KSME C: Technology and Education
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    • v.3 no.3
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    • pp.217-223
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    • 2015
  • The patterning of microbead in microfluidics channel is a practical technique for application in bio and medical areas. An approach is described for a direct patterning of magnetically active microbeads in microfluidic devices without inner structure. Local magnet arrangements - flat arrangement and stack arrangement - contacting same poles or opposite poles of magnet were utilized for generating trapping magnetic fields. The arrangement of magnets contacting same poles generated isolated patterns by repelling of magnetic field. The flat arrangement of vertically reverse magnet arrays shaped trapping patterns repelling magnetic field line between same poles. Spatially, the stack compositions of magnet arrangements allow diverse isolated trapped patterns of magnetic particles. Trapped magnetic particles in fluidic channels were stable on the $18m{\ell}/hr$ flow conditions and magnetic force of 1.08 mT in the all experiments. This experimental study suggests the simple and versatile methods to pattern magnetic particles, and has potential of wide application to bio and medical area.