• 한국가시화정보학회지
• /
• 제18권2호
• /
• pp.10-17
• /
• 2020

The curvature of wound boundaries has been identified as a key modulator that determines a type of force responsible for cell migration. While several studies report how certain curvatures of the boundary correlate with the rate at which the wound closes, it remains unclear how these curvatures are spatiotemporally formed to regulate the healing process. We investigated the dynamic changes in the boundary curvatures by visualizing cell migration patterns. Locally, cells at the convex boundary continuously move forward with transmitting kinetic responses behind to the cells away from the boundary, and cells at the concave boundary exhibit dramatic contracting motion, like a purse-string, when they accumulate enough negative curvatures to gain the thrust toward the void. Globally, the dynamics of boundary geometries are controlled by the diffusive flow of cells driven by the density gradient between the wound area and the cell layer.

• 한국소음진동공학회:학술대회논문집
• /
• 한국소음진동공학회 2006년도 춘계학술대회논문집
• /
• pp.858-861
• /
• 2006

In this paper, a study on the noise reduction in a mobile fuel cell system is presented. Among various fuel cell systems around 20W capacities designed for mobile electronic devices, the active direct methanol fuel cell (DMFC) systems have been recently developed. In such systems, the primary noise source is the air pump which provides sufficient air flow ($5{\sim}6$ liter/min) for electrochemical reaction with methanol fuel while the noise contributions from other auxiliary parts are relatively small. Especially, the discrete noise tones generated by the air pump are dominant and those frequency peaks related to the rotor harmonics are needed to be suppressed by a silencer. Therefore. the Herschel/Quinke (HQ) tubes, which use the out-of-phase cancellation of acoustic waves propagating through direct and indirect pathways, are applied to the inlet of the air pump. Performance of noise reduction with HQ silencer is analytically estimated by calculating the transmission. The length and number of thin HQ tubes are optimized to decrease the radiated noise. As a result, the sound pressure level could be successfully reduced by about 10 dB after applying three serially connected HQ tubes.

• 한국미생물·생명공학회지
• /
• 제22권1호
• /
• pp.80-84
• /
• 1994

In order to induce the rapid alcohol fermentation through the increases of the cell density in a continuous alcohol fermentation of naked barley, the single-cultivation with S. cerevisiae IS-019(SCM, ordinary control), mixed-cultivation with Saccharomyces uvarum IS-026 having a flocculent ability and S. cerevisiae IS-019(MCM), and mash recirculation by single-cultivation of S. cerevisiae IS-019(MRM) modes were investigated. The cell mass in the mixed-cultivation mode was about 10% higher than that of ordinary control but the final alcohol yield was slightlyl decreased. When recycled the mash with the flow rate of 7 l/h from V$_{6}$ to V$_{5}$ fermentors under the ordinary control, the cell density was distributed at 140~170$\times $10$^{6}$ cell/ml depending upon the fermentorsorders, higher about 20% than that of the ordinary control. Under these conditions the alcohol productivity of the maximum and the overall was 12.16 g/l$\cdot $h with an alcohol of 7.6% at the V$_{5}$ fermentor and 1.19 g/l$\cdot $h with an alcohol of 8.94%, respectively. For higher cell mass it was more effective to apply the mash recirculation mode with the single-cultivation of S. cerevisiae IS-019 in a pilot scale multi-stage CSTR.

• Industrial Engineering and Management Systems
• /
• 제15권1호
• /
• pp.11-18
• /
• 2016

This paper considers a scheduling problem in a flexible job shop with a reconfigurable manufacturing cell. The flexible job shop has both operation and routing flexibilities, which can be represented in the form of a multiple process plan, i.e. each part can be processed through alternative operations, each of which can be processed on alternative machines. The scheduling problem has three decision variables: (a) selecting operation/machine pairs for each part; (b) sequencing of parts to be fed into the reconfigurable manufacturing cell; and (c) sequencing of the parts assigned to each machine. Due to the reconfigurable manufacturing cell's ability of adjusting the capacity, functionality and flexibility to the desired levels, the priority scheduling approach is proposed in which the three decisions are made at the same time by combining operation/machine selection rules, input sequencing rules and part sequencing rules. To show the performances of various rule combinations, simulation experiments were done on various instances generated randomly using the experiences of the manufacturing experts, and the results are reported for the objectives of minimizing makespan, mean flow time and mean tardiness, respectively.

• 한국정밀공학회:학술대회논문집
• /
• 한국정밀공학회 2002년도 추계학술대회 논문집
• /
• pp.1055-1058
• /
• 2002

The movement of bone fluid from the region of the bone vasculature through the canaliculi and the lacunae of the surrounding mineralized tissue accomplishes three important tasks. First it transports nutrients to the osteocytes in the lacunae buried in the mineralized matrix. Second, it carries away the cell waste. Third, the bone fluid exerts a force on the cell process, a force that is large enough for the cell to sense. This is probably the basic mechanotrasduction mechanism in bone, the way in which bone senses the mechanical load to which it is subjected. The mechanism of bone fluid flow are described below with particular emphasis on mechanotransduction. Also described is the cell to cell communication by which higher frequency signals might be transferred, a potential mechanism in bone by which the small whole tissue strain is amplified so the bone cells can respond to it. One of the conclusions is that higher frequency low amplitude strains can maintain bone as effectively as low frequency low amplitude strains can maintain bone as effectively as low frequency high amplitude strains. This mechanism has many similarities with the mechanotransduction of acoustical signals in the ear. These conclusion leads to a paradigm shift in how to treat osteoporosis and how to cope with microgravity.

• 대한임상검사과학회지
• /
• 제43권4호
• /
• pp.161-170
• /
• 2011

Quercetin is one of the most distributed flavonoids in the plant kingdom and occurs naturally in a wide range of fruits and vegetables. This study was undertaken to determine whether quercetin exerts beneficial effect against necrotic and apoptotic cell death induced by hydrogen peroxide ($H_2O2$) in intestinal cells using the human-derived cultured T84 colonic epithelial cell line. Necrotic cell death was induced by exposing cells to 0.5 mM $H_2O_2$ for 2 h and apoptosis was induced by incubating cells in normal culture medium for 18 h following exposure of cells to 0.5 mM $H_2O2$ for 2 h. Cell viability was evaluated by the trypan blue exclusion assay and apoptosis was assessed by Hoechst 33258 staining and flow cytometry. $H_2O_2$ induced necrotic cell death in a time and dose-dependent fashion. Both necrotic and apoptotic cell deaths were not prevented by the antioxidants N,N'-diphenyl-p-phenylenediamine(DPPD) and Trolox, whereas both cell deaths induced by the organic hydroperoxide t-butylhydroperoxide (tBHP) were prevented by DPPD, suggesting that $H_2O_2$ induces cell death through a lipid peroxidation-independent mechanism. $H_2O2$-induced necrotic death was prevented by deferoxamine and 3-aminobenzamide, while the apoptotic cell death was not affected by these agents. Quercetin prevented both necrotic and apoptotic cell deaths induced by $H_2O_2$ in a dose-dependent manner. $H_2O_2$ caused activation of poly (ADP-ribose) polmerase (PARP), which was inhibited by deferoxamine, 3-aminobenzamide, and quercetin, but not DPPD. These results indicate that quercetin inhibits both necroticand apoptotic deaths of T84 cells. The anti-necrotic effect of quercetin may be attributed to its iron chelator activity rather than a direct $H_2O_2$ scavenging capacity and antioxidant. The present study suggests that quercetin may play a therapeutic role in the treatment of human gastrointestinal diseases mediated by oxidants.

• Journal of Microbiology and Biotechnology
• /
• 제24권1호
• /
• pp.97-105
• /
• 2014

Quorum quenching (QQ) with a microbial vessel has recently been reported as an economically feasible biofouling control platform in a membrane bioreactor (MBR) for wastewater treatment. In this study, a quorum quenching MBR with a ceramic microbial vessel (CMV) was designed to overcome the extremely low F/M ratio inside a microbial vessel. The CMV was prepared with a monolithic ceramic microporous membrane and AHL-degrading QQ bacteria, Pseudomonas sp. 1A1. The "inner flow feeding mode" was introduced, under which fresh feed was supplied to the MBR only through the center lumen in the CMV. The inner flow feeding mode facilitated nutrient transport to QQ bacteria in the CMV and thus enabled relatively long-term maintenance of cell viability. The quorum quenching effect of the CMV on controlling membrane biofouling in the MBR was more pronounced with the inner flow feeding mode, which was identified by the slower increase in the transmembrane pressure as well as by the visual observation of a biocake that formed on the used membrane surface. In the QQ MBR with the CMV, the concentrations of extracellular polymeric substances were substantially decreased in the biocake on the membrane surface compared with those in the conventional MBR. The CMV also showed its potential with effective biofouling control over long-term operation of the QQ MBR.

• 대한기계학회논문집B
• /
• 제31권7호
• /
• pp.588-595
• /
• 2007

The water transport inside a polymer electrolyte fuel cell (PEFC) varied according to the anodic supply mode. The performance characteristics of a PEFC which can be affected by the water transport were observed with the anodic supply mode. In the flow-through and recirculation mode the performance showed no reduction with time because the flow in the anode was not stagnated. In the dead-end mode, without any discharged gas, the water remains inside of the anode, which caused the reduction of the performance with the lapse of time. However, even in the dead-end mode, little reduction of the performance with time was shown when only the anode was humidified externally. It means that the back-diffusion was the major factor to the accumulation of water in the anode rather than external humidification.

• Plant Biotechnology Reports
• /
• 제4권2호
• /
• pp.117-123
• /
• 2010

We used a flow cytometer to investigate the variations in endopolyploidy (the frequencies of nuclei with DNA contents equivalent to 4C through 16C) during the short period of the early growing stage in vigorously growing young tissues of maize seedlings. We examined different portions of the root and leaves that had been growing for 7 (day 7) and 13 (day 13) days after germination. Endoreplication showed two opposing phenomena without aging. In one case, the endopolyploidy of the first leaf was higher on day 13 than on day 7. In the latter case, endopolyploidy decreased, as clearly revealed by a comparison of the endopolyploidy of the second leaves and the 160-170 mm portion of the seminal root on days 7 and 13. Endopolyploidy was also lower in the top of the leaf. In roots, endopolyploidy was increased by the exogenous application of abscisic acid for only 1 day. The levels of endopolyploidy increased without an increase in cell size in the roots. These results showed that endoreplication occurs in actively growing and young tissue and that the variation can be induced in the short period examined.

• 한국수소및신에너지학회논문집
• /
• 제23권1호
• /
• pp.19-25
• /
• 2012

The efficiency and life time of the Proton Exchange Membrane fuel cell (PEMFC) system is critically affected by incoming gas with humidity which should be maintained properly at normal operating conditions. Typically, incoming gas of automotive fuel cell is humidified by external humidifier but the characteristics of device is rarely reported. In this study, characteristics of water transfer in the membrane humidifiers have been experimentally investigated for flow rates of gas and for different flow arrangement under steady state condition. At first, capability of mass transfer through the membrane is examined at constant temperature. Then, the temperature distribution effect on the capability of mass transfer is tested over various inlet conditions. In summary, this research presents the mass transfer capability of hydrophilic membrane over various operating conditions.