• IMMUNE NETWORK
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• v.9 no.6
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• pp.243-247
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• 2009

In this study, we report the development of a new dual reporter vector system for the analysis of promoter activity. This system employs green fluorescence emitting protein, EGFP, as a reporter, and uses red fluorescence emitting protein, DsRed, as a transfection control in a single vector. The expression of those two proteins can be readily detected via flow cytometry in a single analysis, with no need for any further manipulation after transfection. As this system allows for the simultaneous detection of both the control and reporter proteins in the same cells, only transfected cells which express the control protein, DsRed, can be subjected to promoter activity analysis, via the gating out of all un-transfected cells. This results in a dramatic increase in the promoter activity detection sensitivity. This novel reporter vector system should prove to be a simple and efficient method for the analysis of promoter activity.

• Proceedings of the Korean Society of Crop Science Conference
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• 2019.04a
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• pp.21-21
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• 2019

• Proceedings of the Botanical Society of Korea Conference
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• 1999.08a
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• pp.42-54
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• 1999

• Proceedings of the Korean Society of Crop Science Conference
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• 2018.04a
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• pp.138-138
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• 2018

• Transactions of Materials Processing
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• v.10 no.2
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• pp.151-159
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• 2001

Flow defect of a piston-pin for automobile parts is investigated in this study. In combined cold extrusion of piston-pin, lapping defect, which is a kind of flow defect, appears by the dead metal zone. This appearance evidently happens in products with a thin thickness to be pierced for the dimensional accuracy and the decrease of material loss. The flow defect that occurs in piston-pin has bad effects on the strength and the fatigue life of piston-pin. Therefore, it is important to predict and prevent defects in the early stage of process design. The best method that can prevent flow defect is removing or reducing dead metal zone through material flow control. The finite element simulations are applied to analyze the flow defect. This study proposes processes for preventing flow defect by removing dead metal zone. Then the results are compared with the experimental ones for verification. These FE simulation results are in good agreement with the experimental ones.

• Journal of Microbiology and Biotechnology
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• v.14 no.5
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• pp.1099-1102
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• 2004

Liposomal restructuring induced by hemolytic Listeria monocytogenes was investigated by using flow cytometry. When added to calcein-entrapped liposomes, hemolytic, but not non-hemolytic, Listeria monocytogenes were able to induce reformation of vesicles. Such restructuring of liposomes was easily monitored by flow cytometry. Electron microscopy also indicated major changes in the challenged liposomal structures. The preliminary results described may offer a simple and fast method for monitoring liposomal restructuring and for differentiating between hemolytic and non-hemolytic bacteria.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.26 no.2
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• pp.27-33
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• 2012

In this paper, we researched the heat transfer analysis of 1.2[kW] BLDC motor on the condition of the external flow. The BLDC motor is fallen under influence of some heat generated by rotationg motor speed and it changes the life and confidence of motor. The heat makes to reduce the life of insulation and the magnetization. So motor can't make own performance. Therefore we calculated the losses about number of revolutions of motor. And then, we analyzed the heat transfer of BLDC motor by using the results when a fan attached to motor makes the external flow or not. The results showed that the temperature of motor increased constant values irrespective of external air temperature. The temperature of motor decreased very lower values than prior researches when the fan makes the external flow. In this method we can analyze the performance, losses and heat transfer of motor applied in the many industries.

• Journal of Navigation and Port Research
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• v.38 no.2
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• pp.147-154
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• 2014

The purpose of this study was to identify the correlation among participating in the extreme sports, the flow experience and the satisfaction of life. 420 participants of the extreme sports were selected by the Judgement Sampling Method. Factor analysis and multi-regression analysis have been conducted. The conclusion is as follows. First, the participants' life satisfaction is partially affected by the duration period and term. Second, period, frequency, duration partially effect to life satisfaction. Third, flow experience partially effect to life satisfaction.

• Journal of Biomedical Engineering Research
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• v.36 no.6
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• pp.264-270
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• 2015

A mock circulatory loop system has been developed to construct a simulator for trainees in cardiopulmonary bypass systems or to simulate a test environment for cardiac-assist devices. This paper proposes a computerized mock circulatory loop system whose node is modularized by using a servo control flow regulator to simulate dynamic change of the hemodynamic status. To observe the effect of time-varying resistance, one with hemodynamic properties, the proposed system replicates the planned cross-sectional areas of the outlet of a ventricular assist device in terms of voltage input of a servo valve. The experiment is performed (1) for steady-input commands of selected area sizes and (2) for dynamic commands such as monotonous increase and decrease, and oscillatory functions of the voltage input, and a computer program based on LabVIEW (National Instruments, Austin, USA) processes every measured data and control command to the servo valve. The results show that the pressure and flow at the target points with respect to time-varying resistance match intuitive estimation: the pressure at the outlet and the pressure drop between both sides of the valve increased and the flow at the outlet decreased for increased resistance.

• Journal of The Korean Society of Agricultural Engineers
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• v.62 no.6
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• pp.51-62
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• 2020

The objective of this study was to estimate an optimum ecological flow rate in the Banbyeon stream based on the two representative fish species. Hydraulic stream environment was simulated with HEC-RAS for two water flow regimes and used for the PHABSIM hydraulic simulation. A dominant species of Zacco platypus and an endemic species of Squalidus chankaensis tsuchigae were selected as the representative fishes whose habitat conditions were evaluated for the spawning and adult stages. Weighted usable area (WUA) was estimated based on habitat suitability index (HSI) and PHABSIM habitat simulation. Overall deep water zone in the stream demonstrated greater WUA which implies better habitat status. The estimated WUA for Zacco platypus as the dominant species was about five times greater than Squalidus chankaensis tsuchigae at the stream flow of 12 ㎥/s. The optimum ecological flow rates were 15 ㎥/s and 25 ㎥/s for the respective spawning and adult stages of Zacco platypus, while 5 ㎥/s was estimated for both the life cycles of Squalidus chankaensis tsuchigae. Assuming that the dominant species may survive better in wider flow regimes, the optimum ecological flow rate should be determined rater based on the endemic species and flow rate of 5 ㎥/s was suggested for the Banbyeon stream.