• IMMUNE NETWORK
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• v.12 no.1
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• pp.33-39
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• 2012

Background: Therapeutic approaches using monoclonal antibodies (mAbs) against complement regulatory proteins (CRPs:i.e.,CD46,CD55 and CD59) have been reported for adjuvant cancer therapy. In this study, we generated a recombinant 1E8 single-chain anti-CD59 antibody (scFv-Fc) and tested anti-cancer effect.by using complement dependent cytotoxicity (CDC). Methods: We isolated mRNA from 1E8 hybridoma cells and amplified the variable regions of the heavy chain (VH) and light chain (VL) genes using reversetranscriptase polymerase chain reaction (RT-PCR). Using a linker, the amplified sequences for the heavy and light chains were each connected to the sequence for a single polypeptide chain that was designed to be expressed. The VL and VH fragments were cloned into the pOptiVEC-TOPO vector that contained the human CH2-CH3 fragment. Then, 293T cells were transfected with the 1E8 single-chain Fv-Fc (scFv-Fc) constructs. CD59 expression was evaluated in the prostate cancer cell lines using flow cytometry. The enhancement of CDC effect by mouse 1E8 and 1E8 scFv-Fc were evaluated using a cytotoxicity assay. Results: The scFv-Fc constructs were expressed by the transfected 293T cells and secreted into the culture medium. The immunoreactivity of the secreted scFv-Fc construct was similar to that of the mouse 1E8 for CCRF-CEM cells. The molecular masses of 1E8 scFv-Fc were about 120 kDa and 55 kDa under reducing and non-reducing conditions, respectively. The DNA sequence of 1E8 scFv-Fc was obtained and presented. CD59 was highly expressed by the prostate cancer cell line. The recombinant 1E8 scFv-Fc mAb revealed significantly enhanced CDC effect similar with mouse 1E8 for prostate cancer cells. Conclusion: A 1E8 scFv-Fc construct for adjuvant cancer therapy was developed.

• International Commerce and Information Review
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• v.18 no.3
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• pp.3-21
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• 2016

The thesis examines genealogical study of various aspects to overcome lots of problems which come by when we execute SWIFTNet TSU BPO. Practical implications regarding the innovation of electronic trade infrastructure are as follows. First, the shipping documents in the SWIFTNet TSU BPO are directly sent to an importer by an exporter after the baseline is confirmed. With this process itself, therefore, the bank cannot secure the account receivable. When initiating the SWIFTNet TSU BPO deal, it is needed to set regulations on the bank's account receivable security in the contract. Second, the SWIFTNet TSU BPO should also have an institutionally unified sharing platform with security, stability and convenience. It other words, it is needed to develop services which meet e-payment paradigm and international environments through continued analysis on market changes and flow. Third, the SWIFTNet TSU is useful in terms of promptness, reduction of risk in foreign exchange payment, cost reduction. Therefore, the SWIFT should be perfectly united and linked among the banks, importer and exporter to make the SWIFTNet TSU more convenient in countries around the world. Fourth, the SWIFT should be approached from the aspect of expansion of network and creation of a new business model through analysis on these problems with a worldwide perspective. At the same time, it is necessary to build a cooperative system to share information and promote comprehensive management for efficient operation.

• Journal of Korea Water Resources Association
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• v.36 no.6
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• pp.925-936
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• 2003

The objective of this study is to suggest new drawing methods of isochrones using GIS. For this purpose the Unit Hydrograph (UH) of studied watershed for instantaneous rainfall suggested by Clark have been determined by routing the time-area curve through a single linear reservoir. To evaluate constructing methods of isochrones three methods has been examined; Channel Profile and Clark-kict method; Laurenson method; Average velocity method of S.C.S. Also, these methods have been recomposed by GIS in this study. To apply first method, spatial modeling, the vector based on the stream network and Route_System measuring a distance between points has been used. A raster based on the flow direction grid from burn DEM and the slope grid from original DEM has been applied for the second method. The third method has been applied by a raster based on the landuse grid and a velocity function expressed by slope. Results by these three methods have been evaluated with observed hydrograph, and the method using average velocity method of S.C.S shows more reasonable results comparatively.

• The KIPS Transactions:PartC
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• v.14C no.6
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• pp.493-502
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• 2007

In this paper, we propose a handoff-aware DiffServ scheduler which intends to guarantee various QoS requirements of multimedia services for mobile nodes in TDD/CDMA based wireless networks. TDD is widely used duplexing mechanism in wireless communications. Unlike FDD, TDD allows a node to symmetrically communicate with a base station by using a single frequency band, resulting in high utilization of wireless resources. DiffServ is regarded as a relatively simple QoS support mechanism and thus it is easy to be extended. This is because DiffServ is not a per-flow based mechanisms and it does not require any signaling protocol. However, previously proposed DiffServ schedulers for wired networks can not be deployed directly into wireless networks since they do not consider properties of wireless networks. As a solution to the problem, DSS(DiffServ Supporting Scheduler) was proposed. DSS uses uplink channel, which is originally used for a node to require a base station to transmit packets, to support QoS efficiently. However, QoS does not consider handoff so that it can not support QoS for moving nodes from one cell to the other cell. Therefor. the proposed handoff support QoS mechanism is necessary for TCC/CDMA networks. The proposed scheme allows a mobile node to achieve seamless service without QoS degradation even for the handoff duration.

• Journal of Environmental Science International
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• v.22 no.12
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• pp.1671-1681
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• 2013

The study selected 10 regions among major Korean cities. Then the study classified the yearly change of relative humidity of those regions for 37 years based on 1996 (from 1974 to 2011) aimed at high temperature days, and examined them by stage regarding daily maximum temperature. For large cities and small cities, in general relative humidity had been likely to increase at high temperatures of $30^{\circ}C$ or over before 1996, whereas it has decreased since 1996. For suburban areas, relative humidity had been prone to diminish before 1996, whereas it has been likely to either increase since 1996 or rarely some of the cities have not shown any change. The increasing tendency of relative humidity before 1996 in large cities and small cities is believed to be because of an increase of the latent heat of vaporization by the supply of steam from cooling towers established in downtown areas. Meanwhile, the decreasing tendency from 1996 is concluded to be caused by the change from counter-current circular cooling towers, which produce a great quantity of steam including arsenic acid, to cross-flow cooling towers, which produce hardly any steam containing arsenic acid. This change was in accordance with the modification and pursuit of an urban planning law that ordered cooling towers that had been installed on rooftops be installed in the basement of buildings in consideration of a "Green network creation" project by the Ministry of Environment, urban beautification, concerns since 1996 over building collapses, and according to an argument that steam containing arsenic acid could be harmful to human health owing to chemicals contained in the water in the cooling tower in summer.

• The KIPS Transactions:PartD
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• v.11D no.1
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• pp.105-114
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• 2004

In this paper, we propose a collaboration method of diverse agents each others in multi-agent model and describe a node migration algorithm of Mobile-Agent (MA) using by the metadata of Naming-Agent (NA). Collaboration work of multi-agent assures stability of agent system and provides reliability of information retrieval on the distributed environment. NA, an important part of multi-agent, identifies each agents and series the unique name of each agents, and each agent references the specified object using by its name. Also, NA integrates and manages naming service by agents classification such as Client-Push-Agent (CPA), Server-Push-Agent (SPA), and System-Monitoring-Agent (SMA) based on its characteristic. And, NA provides the location list of mobile nodes to specified MA. Therefore, when MA does move through the nodes, it is needed to improve the efficiency of node migration by specified priority according to hit_count, hit_ratio, node processing and network traffic time. Therefore, in this paper, for the integrated naming service, we design Naming Agent and show the structure of metadata which constructed with fields such as hit_count, hit_ratio, total_count of documents, and so on. And, this paper presents the flow of creation and updating of metadata and the method of node migration with hit_count through the collaboration of multi-agent.

• Journal of radiological science and technology
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• v.36 no.3
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• pp.193-200
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• 2013

There are many concerns about radiation exposure in Korea after Fukushima Nuclear Plant Accident on 2011 in Japan. As some isotope materials are detected in Korea, people get worried about the radioactive material. In addition, the mass media create an air of anxiety that jump on the people's fear instead of scientific approach. Therefore, for curbing this flow, health, medical institute from the world provide a variety of information about medical radiation safety and hold the campaign which can give people the image that medical radiation is safe. At this, the Korean Food and Drug Administration(KFDA) suggested that make the alliance of medical radiation safety and culture on August, 2011. Seven societies and institutions related medical radiation started to research and advertise the culture of medical radiation safety in Korea. In this report, mainly introduce the activities of the Korean Alliance for Radiation Safety and Culture in Medicine(KARSM) for spreading culture of medical radiation safety from 2011 to 2012.

• Archives of design research
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• v.20 no.1 s.69
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• pp.45-54
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• 2007

Recent research regarding local traditions, cultural heritage, and sightseeing resources that represent local characteristics for the purpose of local promotion has been actively advanced. Yangyeongsi in Daegu, South Korea is Doing developed as a core location in order to revitalize regional culture. The unique tradition and functions of this city area have been preserved. Previous research "The Fundamental Research of Revitalization for Yangyeongsi in Daegu for the Local Promotion" undertaken by the author revealed a lack of fundamental research available to establish an understanding of how to revitalize Yangyeongsi. The research methodology designed this by, (1)a site investigation and verification of previous research (2)a deep analysis of Yangyeongsi to uncover potential improvement opportunities (3) assessment of essential elements and appropriate directions for revitalization of the traditional market (4) application of the environment design improvement process to the local design center. The design proposal is that, firstly, space assessment will De improved by the maintenance and expansion of fundamental facilities. Secondly, space application can be maximized by servicing the complex road network through a traffic flow plan. In addition, consideration for the local characteristics will promote unity and identification with the region. Lastly, revitalization and industrialization development of sightseeing resources and secure streets and event spaces will promote enjoyable experiences for visitors. Research results were submitted to the local authority and applied to the future policy plan. Continuous research on revitalization and analysis of the local characteristics are recommended in order to benefit local promotion.

• Journal of the Korean Society for information Management
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• v.35 no.2
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• pp.89-114
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• 2018

Research on changes in research trends in academic disciplines is a method that enables observation of not only the detailed research subject and structure of the field but also the state of change in the flow of time. Therefore, in this study, in order to observe the changes of research trend in library and information science field in Korea, co-word analysis was conducted with Korean author keywords from three types of journals which were listed in the Korea Citation Index(KCI) and have top citation impact factor were selected. For the time series analysis, the 15-year research period was accumulated in 5-years units, and divided into 2003~2007, 2003~2012, and 2003~2017. The keywords which limited to the frequency of appearance 10 or more, respectively, were analyzed and visualized. As a result of the analysis, during the period from 2003 to 2007, the intellectual structure composed with 25 keywords and 8 areas was confirmed, and during the period from 2003 to 2012, the structure composed by 3 areas 17 sub-areas with 76 keywords was confirmed. Also, the intellectual structure during the period from 2003 to 2017 was crowded into 6 areas 32 consisting of a total of 132 keywords. As a result of comprehensive period analysis, in the field of library and information science in Korea, over the past 15 years, new keywords have been added for each period, and detailed topics have also been subdivided and gradually segmented and expanded.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.11
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• pp.817-825
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• 2017

The AC-common bus microgrid system can overcome several weaknesses of the DC microgrid system by interconnecting the DC/AC inverters used for renewable energy with an AC network. Nevertheless, the unbalanced loads inherent in the electric power systems of island and small communities can deteriorate the performance of the AC microgrid system. This is because of the limited voltage regulation capability and mixed power flow in the voltage source inverter. In order to overcome the unbalanced load condition, this paper proposes a voltage and current control algorithm for the 4-leg inverter based on the single phase d-q control method, as well as the modeling of the voltage controller using Matlab/Simulink S/W. From the S/W simulation and experiment of the 250KW proto-type inverter, it is confirmed that the proposed algorithm is a useful tool for the design and operation of the AC microgrid system.