• Title/Summary/Keyword: Flow Measurements

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Changes in Serum IGF-I and Spermatogenesis Analysed by Flow Cytometry in Growing Male Rabbit (성장 중인 수토끼에서 혈청 IGF-I 수준과 Flow Cytometry 측정에 의한 정자 형성의 변화)

  • Lee J. H.;Kim C. K.;Chang Y. M.;Ryu J. W.;Park M. Y.;Chung Y. C.;Pang M. G.
    • Reproductive and Developmental Biology
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    • v.29 no.3
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    • pp.163-168
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    • 2005
  • The aim of this study was to investigate the changes in insulin-like growth factor-I (IGF-I) and growth hormone (GH) in serum, the quantitation of spermato-genesis and the comparable relationships among these measurements during pubertal period in New Zealand White male rabbits. To investigate the age-related testicular changes in DNA contents of spermatogenic cells, the fine-needle testicular biopsies from males aged 10 to 28 wks were evaluated by flow cytometry(FCM). Body weight increased significantly between the ages of 12 and 20 wks (P<0.05) and reached 3.4 kg at 28 wks of age. The highest serum IGF-I level (451.3ng/mL) was observed at 20wks of age (P<0.05) and thereafter remained stable at low levels. Serum GH level at 18 wks of age was 183.3 pg/mL which was significantly higher compared to the other ages (P<0.05), and the rising time in serum GH tend to be somewhat earlier than that of IGF-I. The relative percentage of It-cells in testicular cell compartments was $48.2\%$ at the age of 18 wks which significantly increased than those of 16-wk-old (P<0.05) and thereafter increased with the advance of age to $68\%$. The percentage of 2C-cells in testis was $26.8\%$ at 18 wks of age which was significantly lower than $54.3\%$ at 16 wks old (P<0.05). The percentage of 4C-cells was constantly maintained $2\~6\%$ except the $9.9\%$ at 18 wks of age. In conclusion, the results suggest that the puberty onset occurred at about the 18 wks of age and that the IGF-I and GH in serum during the pubertal period showed the age/growth-specific changes and these changes might be related to the spermatogenesis. The DNA FCM combined with fine-needle testicular biopsy could offer a very sensitive method to monitor the quantitative spermatogenic events related to the puberty onset.

A Study on the Application of Gastrodiae rhizoma for Food Stuffs - Effects of Gastrodiae rhizoma on the Regional Cerebral Blood Flow and Blood Pressure - (천마의 식품학적 활용을 위한 기초 연구 - 포제천마 열수 추출물이 국소 뇌혈류량과 혈압에 미치는 영향 -)

  • Park, Sung-Hye;Cho, Choa-Hyoung;Ahn, Byung-Yong
    • Journal of the East Asian Society of Dietary Life
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    • v.17 no.4
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    • pp.554-562
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    • 2007
  • This study was performed to provide basic data for predicting the usefulness of Gastrodiae rhizoma as a materials for functional foods. Changes in regional cerebral blood flow(rCBF) and blood pressure(BP) were measured in rats, following the intravenous injection of processed Gastrodiae rhizoma water extract. In its processing, we used rice water, Sderotium Poriae Cocos and Radix Ligustici Chuanxiaong. The rCBF and BP measurements were continually monitored by a laser-doppler flowmeter and a pressure transducer in the anesthetized adult Sprague-Dawley rats for approximately about two to two and a half hours, through a data acquisition system composed of a MacLab and Macintosh computer. The results of the experiment are as follows: the processed Gastrodiae rhizoma significantly increased changes in rCBF in the rats. The rCBF with processed Gastrodiae rhizoma did not change by pretreatment with propranolol, atropin, methylene blue, and indomethacin. But the rCBF of the processed Gastrodiae rhizoma was increased by pretreatment with L-NNA. The processed Gastrodiae rhizoma significantly decreased the changes in BP. However, BP with the processed Gastrodiae rhizoma did not change by pretreatment with propranolol, atropin, methylene blue and indomethacin. On the other hand, BP decreased with the processed Gastrodiae rhizoma pretreatment with L-NNA. These results indicate that processed Gastrodiae rhizoma might increase the rCBF and the BP which are related to nitric oxide synthesis. Also these results indicate that the used of processed Gastrodiae rhizoma in safe, as well as clinically applicable in diet therapy for cerebral related disease and hypertension.

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Error Analysis of Image Velocimetry According to the Variation of the Interrogation Area (상관영역 크기 변화에 따른 영상유속계의 오차 분석)

  • Kim, Seojun;Yu, Kwonkyu;Yoon, Byungman
    • Journal of Korea Water Resources Association
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    • v.46 no.8
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    • pp.821-831
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    • 2013
  • Recently image velocimetries, including particle image velocimetry (PIV) and surface image velocimetry (SIV), are often used to measure flow velocities in laboratories and rivers. The most difficult point in using image velocimetries may be how to determine the sizes of the interrogation areas and the measurement uncertainties. Especially, it is a little hard for unskilled users to use these instruments, since any standardized measuring techniques or measurement uncertainties are not well evaluated. Sometimes the user's skill and understanding on the instruments may make a wide gap between velocity measurement results. The present study aims to evaluate image velocimetry's uncertainties due to the changes in the sizes of interrogation areas and searching areas with the error analyses. For the purpose, we generated 12 series of artificial images with known velocity fields and various numbers and sizes of particles. The analysis results showed that the accuracy of velocity measurements of the image velocimetry was significantly affected by the change of the size of interrogation area. Generally speaking, the error was reduced as the size of interrogation areas became small. For the same sizes of interrogation areas, the larger particle sizes and the larger number of particles resulted smaller errors. Especially, the errors of the image velocimetries were more affected by the number of particles rather than the sizes of them. As the sizes of interrogation areas were increased, the differences between the maximum and the minimum errors seemed to be reduced. For the size of the interrogation area whose average errors were less than 5%, the differences between the maximum and the minimum errors seemed a little large. For the case, in other words, the uncertainty of the velocity measurements of the image velocimetry was large. In the viewpoint of the particle density, the size of the interrogation area was small for large particle density cases. For the cases of large number of particle and small particle density, however, the minimum size of interrogation area became smaller.

An Oceanic Current Map of the East Sea for Science Textbooks Based on Scientific Knowledge Acquired from Oceanic Measurements (해양관측을 통해 획득된 과학적 지식에 기반한 과학교과서 동해 해류도)

  • Park, Kyung-Ae;Park, Ji-Eun;Choi, Byoung-Ju;Byun, Do-Seong;Lee, Eun-Il
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.18 no.4
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    • pp.234-265
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    • 2013
  • Oceanic current maps in the secondary school science and earth science textbooks have played an important role in piquing students's inquisitiveness and interests in the ocean. Such maps can provide students with important opportunities to learn about oceanic currents relevant to abrupt climate change and global energy balance issues. Nevertheless, serious and diverse errors in these secondary school oceanic current maps have been discovered upon comparison with up-to-date scientific knowledge concerning oceanic currents. This study presents the fundamental methods and strategies for constructing such maps error-free, through the unification of the diverse current maps currently in the textbooks. In order to do so, we analyzed the maps found in 27 different textbooks and compared them with other up-to-date maps found in scientific journals, and developed a mapping technique for extracting digitalized quantitative information on warm and cold currents in the East Sea. We devised analysis items for the current visualization in relation to the branching features of the Tsushima Warm Current (TWC) in the Korea Strait. These analysis items include: its nearshore and offshore branches, the northern limit and distance from the coast of the East Korea Warm Current, outflow features of the TWC near the Tsugaru and Soya Straits and their returning currents, and flow patterns of the Liman Cold Current and the North Korea Cold Current. The first draft of the current map was constructed based upon the scientific knowledge and input of oceanographers based on oceanic in-situ measurements, and was corrected with the help of a questionnaire survey to the members of an oceanographic society. In addition, diverse comments have been collected from a special session of the 2013 spring meeting of the Korean Oceanographic Society to assist in the construction of an accurate current map of the East Sea which has been corrected repeatedly through in-depth discussions with oceanographers. Finally, we have obtained constructive comments and evaluations of the interim version of the current map from several well-known ocean current experts and incorporated their input to complete the map's final version. To avoid errors in the production of oceanic current maps in future textbooks, we provide the geolocation information (latitude and longitude) of the currents by digitalizing the map. This study is expected to be the first step towards the completion of an oceanographic current map suitable for secondary school textbooks, and to encourage oceanographers to take more interest in oceanic education.

Characteristics of Seepage Water and Groundwater in a Coastal LPG Storage Cavern of Jeonnam (전남 해안 LPG 저장공동 유출수와 주변 지하수의 수질특성)

  • Lee, Jin-Yong;Choi, Mi-Jung;Kim, Hyun-Jung;Cho, Byung-Wook
    • Journal of Soil and Groundwater Environment
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    • v.14 no.4
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    • pp.33-44
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    • 2009
  • Water curtain of an underground LPG storage cavern is a facility to prevent leakage of high pressure gases, for which groundwater should flow freely towards the cavern and groundwater level also must be stably maintained. In this study, in order to evaluate qualities of seepage water and surrounding groundwater of an underground LPG storage cavern in Yeosu, 4 rounds of samplings, field measurements and laboratory analyses (February, May, August, October of 2007) were conducted. According to field measurements, pH was weak acidic to neutral but it gradually increased with time. Electrical conductivity (EC) of groundwater near a salt stack showed very high values between 10.47 and 38.50 mS/cm. Dissolved oxygen (DO) showed a very wide range of 0.20~8.74 mg/L and a mean of oxidation-reduction potential (ORP) was 159 mV, which indicated an oxidized condition. Levels of $Fe^{2+}$ and $Mn^{2+}$ were mostly less than 3 mg/L. All of seepage waters showed a Na-Cl type while only groundwater near the salt stack showed a Na-Cl type with a high total dissolved solid. The other groundwaters exhibited typical $Ca-HCO_3$ types. Levels of aerobic bacteria were mostly very high (573-39,520 CFU/mL). Based on the analyses of these hydrochemistry and biological characteristics, it is concluded that there are no particular problems in groundwater and seepage water, which not causing a trouble in the cavern operation. However, both for control of bio-clogging and for sustainable operation of the water curtain system, a regular hydrochemical and microbiological monitoring is required for the seepage water and surrounding groundwater.

Characterization of Groundwater Quality and Recharge using Periodic Measurements of Hydrogeochemical Parameters and Environmental Tracers in Basaltic Aquifers of Jeju Island (수리지구화학적 인자와 환경 추적자의 주기적 관측에 의한 제주도 지하수의 수질과 함양 특성 분석)

  • Koh, Dong-Chan;Cheon, Su-Hyun;Park, Ki-Hwa
    • Journal of Soil and Groundwater Environment
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    • v.12 no.4
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    • pp.60-71
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    • 2007
  • Groundwater from public wells was monitored during one year with two month interval for hydrogeochemical parameters and chlorofluorocarbons (CFCs) as environmental tracers in Jeju Island. Concentrations of major cations and $SiO_2$ show variation less than 10% whereas $NO_3$ and dissolved oxygen (DO) showed larger variation though DO variation did not change oxic or suboxic condition. $NO_3$ concentration has no consistent seasonal pattern with the largest variation of 35%. Groundwater ages determined by CFCs became temporarily younger by 5 years in October for groundwater with ages of 15 to 25 years, which can be attributed to infiltrating water in rainy season. Compared to air temperature, groundwater temperature has much smaller variation with no phase difference, which can be accounted for by a two-component model consisting of infiltrating water from surface and deeper groundwater with negligible temperature variation. The relatively small variation in groundwater age and temperature indicates that groundwater recharge through fast flow-paths is much smaller compared with basal groundwater in terms of aquifer storage.

Prediction of Continuous Discharge and Water Quality Change for Gate Operation in Seonakdong River Experimental Catchment Using SWAT (서낙동강 시험유역에서의 SWAT 모형을 이용한 수문 운영에 따른 연속유출 및 수질변화 예측)

  • Kang, Deok-Ho;Kim, Jung-Min;Kim, Tae-Won;Kim, Young-Do
    • Journal of Wetlands Research
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    • v.14 no.1
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    • pp.21-33
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    • 2012
  • The dominant land use at the Seonakdong river watershed is paddy and forest areas and the Seonakdong river stands still. Thus, the water pollution in the Seonakdong river is becoming more serious for the non-point source. In this study, SWAT(Soil and Water Assessment Tool) model was evaluated for simulation of flow and water quality behaviors in Seonakdong river. To perform the calibration and verification of the SWAT model, the measurements of discharge and water quality were performed for the period from 2006 to 2007 at 5 gauging stations in Seonakdong river. The $R^2$ value for discharge and water quality were 0.86 and 0.70 respectively for calibration after the sensitive analysis. The $R^2$ value for discharge and water quality were 0.81 and 0.51 respectively for verification. The simulation results show that BOD value in the river tends to decrease after the opening of gates and the patterns of TN and TP concentrations are similar as that of BOD. The gate operators need to determine how to supply water in drought season for effective water quality improvement. This study shows that the SWAT model, which is capable of simulating hydrologic and water quality behaviors temporarily and spatially at watershed scale, could be used to get the gate operation rule for the water quality management in Seonakdong river.

Experimental Study on Underwater Transient Noise Generated by Water-Entry Impact (입수 충격 수중 순간 소음에 대한 실험적 연구)

  • Jung, Youngcheol;Seong, Woojae;Lee, Keunhwa;Kim, Hyoungrok
    • The Journal of the Acoustical Society of Korea
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    • v.33 no.1
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    • pp.10-20
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    • 2014
  • To study the water-entry impact noise, on-board experiment using a small launcher firing various objects was performed in the Yellow Sea. As the launcher fires a cylindrical object from the ship vertically, generated noise is measured with a hydrophone on the starboard of Chung-hae, Marine surveyor. Three types of cylindrical objects, which have noses of flat-faced, conical, and hemisphere, were used during the experiment. The measured noise exhibits a time-dependency which can be divided into three phases: (1) initial impact phase, (2) open cavity flow phase, (3) cavity collapse and bubble oscillation phase. In most cases, the waveform of bubble oscillation phase is dominant rather than that of initial impact phase. Pinch-off time, where a cavity begins to collapse, occurs at 0.18 ~ 0.2 second and the average lasting time of bubble was 0.9 ~ 1.3 second. The energy of water-entry impact noise is focused in the frequency region lower than 100 Hz, and the generated noise is influenced by the nose shapes, object mass, and launching velocity. As a result, energy spectral density on the bubble frequency is higher in the order of flat-faced, conical, hemisphere nose, and the increase of initial energy raises the energy spectral density on the bubble frequency in the cylinder body of same shape. Finally, we compare the measurements with the simulated signals and spectrum based on the bubble explosion physics, and obtain satisfactory agreements between them.

Effect of human biosignal according as foot was heating with hot water (발 부분 온수 열자극시 인체 현상에 미치는 영향)

  • Lee, Woo-Cheol;Min, Kyeug-Kee;SaKong, Sug-Chin
    • 전자공학회논문지 IE
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    • v.43 no.1
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    • pp.5-15
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    • 2006
  • In this paper, we measured the biosignal using hot-water system(STYX ford202) on foot. The biosignal transition data is observed from hot-water thermotherapy on foot. pre and post demonstration conditions under 43$^{\circ}C$ and 45$^{\circ}C$ are checked about 9 physiological factors for 10 persons and 10 days. (Checking Time: pre-test, post-test(5, 10, 15, 20 minutes)). The biosignal transition of demonstration's results showed as belows; Forehead Temperature($^{\circ}C$): -0.69 $\pm$ 0.01 dec, Leg Temperature($^{\circ}C$): 1.51 $\pm$ 0.22 inc, Blood Flow($m\ell/min$): 1.18 $\pm$ 0.50 inc, Blood Pressure(mmHg): (max) -1.49$\pm$ 2.81, (min) -0.06 $\pm$ 0.13 dec, Heart Rate(bpm): 6.97 $\pm$ 0.72 inc, Blood Sugar($mg/d\ell$) : -2.41 $\pm$ 1.55 dec, Oxygen Saturation(%): 1.34 $\pm$ 0.28 inc, Body Fat(%) -1.75 $\pm$ 0.15 dec, Weight(kg): -0.10 $\pm$ 0.04 dec. (dec: decrease, inc: increase)

Numerical Analysis of the Stability of a Tailings Dump Slope using Field Monitoring Results (현장 모니터링 결과를 이용한 광미 적치사면 안정성의 수치해석적 연구)

  • Song, Young-Suk
    • The Journal of Engineering Geology
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    • v.27 no.1
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    • pp.21-29
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    • 2017
  • Variations in matric suction were compared between field measurements and numerical analysis for a tailings dump slope. We performed an analysis of slope stability using precipitation records measured in the field, selecting the period (72 hours) of highest rainfall intensity during the field monitoring, for which the matric suction and rainfall records measured in the field were analyzed. In addition, we applied the precipitation records of this period to the numerical analysis. SEEP/W and SLOPE/W were used to analyze the seepage flow in the slope due to rainfall and to investigate the slope stability considering the wetting front depth from the ground surface, respectively. The seepage analysis, using SEEP/W, was carried out on the rainfall data obtained in the field. Comparisons between the field monitoring data and simulation results for matric suction show some quantitative difference but similar patterns of temporal variation. According to the results of slope stability analysis using SLOPE/W and the results of seepage analysis, the safety factor of the slope showed a sudden increase at the point of rapid increase in rainfall intensity. It then recovered because subsequent rainfall was scarce. Therefore, the stability of the tailings dump slope can be reasonably estimated if seepage and slope stability analyses, based on precipitation records, have been carried out.