• 대한화학회지
• /
• 제63권6호
• /
• pp.427-434
• /
• 2019

단백질 폴딩 연구에 유용하도록 유비퀴틴 단백질의 페닐알라닌 45를 트립토판으로, 발린 26을 알라닌으로 변이시킨 HubWA 단백질을 모델로 삼아 소수성 상호작용이 단백질 폴딩 반응에 끼치는 영향을 탐구하였다. HubWA의 소수성 아미노산 중 14 개를 알라닌으로 치환한 변이 단백질을 제조하였고 이들 중 폴딩 연구에 적절한 4 개의 변이 단백질(V5A, I13A, V17A, I36A)을 얻어서 폴딩 반응의 진행 과정을 stopped-flow 장치로 측정하였다. 변이 단백질 V17A의 폴딩 반응은 HubWA와 마찬가지로 three-state 메커니즘을 따르며, V5A, I13A, I36A의 반응은 two-state 폴딩 메커니즘을 따르는 것으로 관찰되었다. 이는 HubWA 단백질의 폴딩 반응은 지엽적으로 구조적인 안정성을 지닌 부분이 존재하는 중간 단계가 먼저 형성된 다음 이들이 서로 퍼즐을 맞추는 것과 같은 방식으로 폴딩이 일어나는 collision-diffusion 메커니즘을 따르다가 소수성이 약한 아미노산으로 치환하였을 때 구조적인 안정성을 지닌 중간 단계가 관찰되지 않지만 폴딩 핵의 형성과 핵 주위로 native 구조가 형성되는 반응이 짝지어서 일어나는 nucleation-condensation 메커니즘으로 전환되는 것으로 해석되었다. 이러한 관찰은 단백질의 폴딩 경로는 지엽적인 구조의 안정성에 따라 서로 다른 메커니즘을 띨 수 있음을 시사한다.

• 제어로봇시스템학회논문지
• /
• 제22권3호
• /
• pp.210-216
• /
• 2016

This paper presents a novel collision avoidance technique for mobile robots based on omni-directional vision simultaneous localization and mapping (SLAM). This method estimates the avoidance path and speed of a robot from the location of an obstacle, which can be detected using the Lucas-Kanade Optical Flow in images obtained through fish-eye cameras mounted on the robots. The conventional methods suggest avoidance paths by constructing an arbitrary force field around the obstacle found in the complete map obtained through the SLAM. Robots can also avoid obstacles by using the speed command based on the robot modeling and curved movement path of the robot. The recent research has been improved by optimizing the algorithm for the actual robot. However, research related to a robot using omni-directional vision SLAM to acquire around information at once has been comparatively less studied. The robot with the proposed algorithm avoids obstacles according to the estimated avoidance path based on the map obtained through an omni-directional vision SLAM using a fisheye image, and returns to the original path. In particular, it avoids the obstacles with various speed and direction using acceleration components based on motion information obtained by analyzing around the obstacles. The experimental results confirm the reliability of an avoidance algorithm through comparison between position obtained by the proposed algorithm and the real position collected while avoiding the obstacles.

• 한국수자원학회논문집
• /
• 제54권6호
• /
• pp.407-418
• /
• 2021

본 연구에서는 유목의 유입량의 특성과 유목줄기의 길이에 따른 하천흐름과 유목거동을 분석하기 위해 2차원 흐름모형인 Nays2DH와 유목동역학모형을 결합하고 이를 활용하여 장애물 근처에서의 유목의 군집특성과 거동을 분석하였다. 또한 유목의 충돌거동을 모의하기 위해 유목동역학모형에 Dashpot-spring 모형을 추가하여 유목의 충돌에 따른 군집특성도 비교하였다. 모의결과 유목줄기의 길이에 따라 장애물구간의 통과율, 유목의 군집이송, 유목들의 평균위치가 민감하게 반응하였고 유목의 유입규모에 대해서는 통과율 이외에는 미미한 반응을 나타냈다. 본 연구를 통해 수공구조물과 유목의 상호작용을 이해할 수 있었으며 이를 통해 유목의 이송과 군집현상 등을 사전에 예측함으로써 수공구조물의 내구성 유지방안 구축에 도움이 될 것으로 사료된다.

• 대한교통학회지
• /
• 제29권3호
• /
• pp.73-82
• /
• 2011

최근 교통상황을 정확하게 관측할 수 있는 교통류 검지에 관한 기술개발과 더불어 개별차량 주행궤적을 이용한 교통안전도 평가기법에 대한 관심이 높아지고 있다. 본 연구에서는 개별차량의 주행궤적을 이용하여 다음시점(t+1) 의 후미추돌 확률을 산출하는 방법론을 제시하였다. 신뢰성 있는 예측 기법인 칼만 필터링(Kalman Filtering)을 이용하여 주행궤적을 예측하고, 예측된 시점에 대한 개별차량의 후미추돌 확률을 산출하였다. 안전도를 평가하는 확률모형을 수립하기 위해서 서해안 고속도로의 동영상 자료로부터 개별차량의 주행궤적을 추출하였다. 추출한 개별차량의 주행궤적 자료를 이항 로지스틱 회귀분석(Binary logistic regression)을 이용하여 차량의 차로변경 결정 확률 모형을 생성하였고, exponential decay function을 이용하여 surrogate safety measure(SSM)의 하나인 time-to-collision(TTC)기반의 추돌확률 모형을 생성하였다. 미시적 교통류 시뮬레이터인 VISSIM에서 추출한 개별차량의 주행궤적 데이터를 이용하여 제안된 방법론을 평가하였다. 본 연구의 결과는 교통류 감시, 제어 및 정보 시스템에 효과적으로 적용될 수 있으며, 나아가 교통사고 예방에 효율적인 대안이 될 수 있을 것으로 판단된다.

• 한국전산유체공학회:학술대회논문집
• /
• 한국전산유체공학회 2005년도 추계 학술대회논문집
• /
• pp.11-19
• /
• 2005

First, methods of numerical analysis of gas-particle flows is classified into micro, meso and macro scale approaches based on the concept of multi-scale mechanics. Next, the explanation moves on to discrete particle simulation where motion of individual particles is calculated numerically using the Newtonian equations of motion. The author focuses on the cases where particle-to-particle interaction has significant effects on the phenomena. Concerning the particle-to-particle interaction, two cases are considered: the one is collision-dominated flows and the other is the contact-dominated flows. To treat this interaction mathematically, techniques named DEM(Distinct Element Method) or DSMC (Direct Simulation Monte Carlo) have been developed DEM, which has been developed in the field of soil mechanics, is useful for the contact -dominated flows and DSMC method, developed in molecular gas flows, is for the collision-dominated flows. Combining DEM or DSMC with CFD (computer fluid dynamics), the discrete particle simulation becomes a more practical tool for industrial flows because not only the particle-particle interaction but particle-fluid interaction can be handled. As examples of simulations, various results are shown, such as hopper flows, particle segregation phenomena, particle mixing in a rotating drum, dense phase pneumatic conveying, spouted bed, dense phase fluidized bed, fast circulating fluidized bed and so on.

• Wind and Structures
• /
• 제23권6호
• /
• pp.523-542
• /
• 2016

Snowdrift formation on roofs should be considered in snowy and windy areas to ensure the safety of buildings. Presently, the prediction of snowdrifts on roofs relies heavily on field measurements, wind tunnel tests and numerical simulations. In this paper, a new snowdrift modeling method by using CFD (Computational Fluid Dynamics) coupled with DEM (Discrete Element Method) is presented, including material parameters and particle size, collision parameters, particle numbers and input modes, boundary conditions of CFD, simulation time and inlet velocity, and coupling calculation process. Not only is the two-way coupling between wind and snow particles which includes the transient changes in snow surface topography, but also the cohesion and collision between snow particles are taken into account. The numerical method is applied to simulate the snowdrift on a typical stepped flat roof. The feasibility of using coupled CFD with DEM to study snowdrift is verified by comparing the simulation results with field measurement results on the snow depth distribution of the lower roof.

• 대한조선학회논문집
• /
• 제54권1호
• /
• pp.26-33
• /
• 2017

In this study, the surface marker method, one of the particle tracking methods, used to track the free surface is extended to cover the more general cases easily including the collision and separation of the free surface. In surface particle method to redistribute particles effectively using the grid system, the free surface is composed of the sum of quadrilaterals having four curves where fixed markers are placed at ends of each curve. Fixed markers are used to know how curves are connected to each other. The position of fixed markers can move as the free surface deforms but all fixed markers cannot be deleted during all time of simulation to keep informations of curve connection. In the case of the collision or separtion of the free surface where several curves can be intersected disorderly, severe difficulties can occur to define newly states of curve connection. In this study, the adaptable surface parTicle method without fixed markers is introduced. Intersection markers instead of the fixed markers are used to define quadrilaterals. The position of the intersection markers is defined to be the intersection point between the free surface and the edge of the grid and it can be added or deleted during the time of simulation to allow more flexibilities. To verify numerical schemes, two flow cases are simulated and the numerical results are compared with other's one and shown to be valid.

• 한국자동차공학회논문집
• /
• 제19권6호
• /
• pp.61-67
• /
• 2011

The cooling module needs enough space (or distance) from hood to absorb the energy from any pedestrian collision. Downsized cooling module for pedestrian protection is important to reduce the severity of pedestrian injury. When a vehicle collision happens, the downsized cooling module is required to reduce the risk of injury to the upper legs of adults and the heads of children. In this study, the performance of cooling module to cool the engine was investigated under 25% height reduction. The heat dissipation and pressure drop characteristics have been experimentally studied with the variation of coolant flow rate, air inlet velocity and A/C operation ON/OFF for the downsized cooling module. The results indicated that the cooling performance was about 94% level compared to that of the conventional cooling module. Therefore, we checked that the cooling module had good performance, and expected that the cooling module could meet the same cooling performance as conventional cooling module through optimization of components efficiency.

• 상하수도학회지
• /
• 제18권2호
• /
• pp.235-241
• /
• 2004

The dissolved air flotation (DAF) process has been widely used for removing suspended solids with low density in water. It has been known as measuring the size of microbubbles precisely which move upward rapidly in contact zone is difficult. In this study particle counter monitoring (PCM) method is used to measure the rising microbubble after injection from a nozzle. Size and distribution curve of microbubbles are evaluated at different conditions such as pressure drop at intermediate valve, length of pipeline between saturation tank and nozzle and low pressure. And the efficiency is also checked when it collides with different size floc. The experimental results show the following fact. As the final pressure drop occurred closer to a nozzle, the bubble size became smaller. And small bubble collides with large floc as well as small one because of its physical characteristic. However large bubble collides well with large floc rather than small one since hydrodynamic flow in streamline interferes to collide between two. With performing computational process by mathematical model we have analyzed and verified the size effect between bubble and floc. Collision efficiency is the highest when P/B ratio shows in the range of 0.75 < P/B ratio ($R_{particle/Rbubble}$) < 2.0.

• 한국지능시스템학회논문지
• /
• 제24권6호
• /
• pp.603-608
• /
• 2014

해상교통환경에서 선박간 근접사고(Near-miss)는 양 선박이 충돌 위험코스로 서로 근접하여 충돌에 임박한 상황으로 실제 충돌은 발생되지 않은 사고를 말한다. 본 연구에서는 통항 선박들간 근접사고 산출을 위해 선박 범퍼 영역모델을 활용한 근접사고 판별식과 선박 위치 클러스터링을 통해 해역의 근접사고 산출 모듈을 제안하고자 한다. 제안된 근접사고 산출 모듈을 완도해역 통항선박 항적데이터에 적용하여 선종, 항행속력 및 조우방향 등 선박 항해 위험 요인을 평가하고자 한다.