• Korean Journal of Construction Engineering and Management
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• v.9 no.3
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• pp.126-135
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• 2008

The purpose of this study is to develop the efficient information system for managing owner's requirement(O/R) throughout the design phases. In order to derive an improvement, current status of O/R management both in domestic and foreign design company has been analyzed. Through the literature reviews and interviews with experts, the Information on design process and detailed activities were investigated. Based on the basic research, inputs, outputs and participants were defined and information flow diagram was drawn. O/R's were categorized according to comprehensive interviews with specialists, and a prototype system was suggested. Conducting systematized O/R management will improve the confidence of owners' role on design, reduce re-work in design tasks, and finally elevate design quality.

• The Korean journal of internal medicine
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• v.34 no.1
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• pp.146-155
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• 2019

Background/Aims: Indoxyl sulfate (IS) is a uremic toxin and an important causative factor in the progression of chronic kidney disease. Recently, paricalcitol (19-nor-1,25-dihydroxyvitamin D2) was shown to exhibit protective effects in kidney injury. Here, we investigated the effects of paricalcitol treatment on IS-induced renal tubular injury. Methods: The fluorescent dye 2',7'-dichlorofluorescein diacetate was used to measure intracellular reactive oxygen species (ROS) following IS administration in human renal proximal tubular epithelial (HK-2) cells. The effects of IS on cell viability were determined using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays and levels of apoptosis-related proteins (Bcl-2-associated protein X [Bax] and B-cell lymphoma 2 [Bcl-2]), nuclear $factor-{\kappa}B$ ($NF-{\kappa}B$) p65, and phosphorylation of mitogen-activated protein kinase (MAPK) and protein kinase B (Akt) were determined by semiquantitative immunoblotting. The promoter activity of $NF-{\kappa}B$ was measured by luciferase assays and apoptosis was determined by f low cytometry of cells stained with f luorescein isothiocyanate-conjugated Annexin V protein. Results: IS treatment increased ROS production, decreased cell viability and induced apoptosis in HK-2 cells. IS treatment increased the expression of apoptosis-related protein Bax, decreased Bcl-2 expression, and activated phosphorylation of MAPK, $NF-{\kappa}B$ p65, and Akt. In contrast, paricalcitol treatment decreased Bax expression, increased Bcl-2 expression, and inhibited phosphorylation of MAPK, $NF-{\kappa}B$ p65, and Akt in HK-2 cells. $NF-{\kappa}B$ promoter activity was increased following IS, administration and was counteracted by pretreatment with paricalcitol. Additionally, flow cytometry analysis revealed that IS-induced apoptosis was attenuated by paricalcitol treatment, which resulted in decreased numbers of fluorescein isothiocyanate-conjugated Annexin V positive cells. Conclusions: Treatment with paricalcitol inhibited IS-induced apoptosis by regulating MAPK, $NF-{\kappa}B$, and Akt signaling pathway in HK-2 cells.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.107-107
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• 2019

하천은 과거부터 현재까지 인간의 삶에 있어서 중요한 공간이며 환경적, 생태적으로도 중요한 가치를 지니고 있다. 특히, 하천은 물과 흙으로 이루어져 있기 때문에 수생물이 살아갈 수 있는 독특한 생태 서식처 공간을 제공한다. 국내에서는 1980년대 말부터 하천환경의 개선 및 복원에 대해 관심과 인식을 높이고 공감대를 형성하여 훼손된 하천을 원래 모습으로 되돌리는 사업을 수행하였다. 이러한 하천복원사업이 하천에 서식하는 수생물의 어떠한 영향을 주는지 분석하기 위해 최근에는 다양한 물리서식처 분석이 수행되고 이를 통해 각종 복원계획을 수립하고 있다. 하천복원사업을 통한 생태유량 재산정, 보 철거의 영향, 다양한 복원기법을 통한 수생물에 미치는 영향 파악 등을 들 수 있다. 그러나 이러한 기존 연구들은 하천복원사업으로 인한 수리특성과 수생 서식처의 변화를 살펴보는 간접적인 영향에 대한 내용이었다. 그러나 직접적으로 영향을 미치는 하도의 단면을 재설계하고 다양한 복원기법을 적용하여 그 영향을 파악하는 연구도 수행되어야 할 것으로 판단된다. 본 연구는 수리학적으로 유리한 저수로 물길 조성 및 다양한 저수로 조성기법의 적용을 통해 우점종의 서식처에 미치는 영향에 대해 원주천을 대상으로 물리서식처 분석을 수행하였다. 대상구간은 원주천 중 상류에 위치한 900 m 구간으로, 대상 어종은 원주천의 중 상류에 서식하는 우점종인 참갈겨니를 목표로 하였다. 수리분석과 서식처분석은 각각 River2D 모형과 서식처 적합도 모형을 사용하였다. 서식처 적합도 모형에 사용된 서식처 적합도 곡선은 Gosse (1982)의 방법을 이용하여 구축하였으며, 수심, 유속, 기층을 모두 고려하여 물리 서식처 분석을 수행하였다. 저수로 물길 복원 조성을 위하여 수리학적으로 유리한 저수로 단면으로의 복원 및 거석, 여울/소 구조, 단면의 확폭을 통해 기존 자연형 하천과는 다른 복원을 조성하였다. 수리학적으로 유리한 저수로 물길 복원을 조성한 결과를 이용하여 물리서식처 분석을 수행하였을 때, 기존 자연하천인 현상태 대비 약 37% 가중가용면적이 악화되는 것으로 나타났다. 그러나 수리학적으로 유리한 저수로 단면의 설계 조건에서 거석, 여울/소 구조 및 단면의 확폭의 복원을 수행하였을 때 서식처가 최소 약 3%에서 최대 21%까지 가중가용면적이 향상되었다.

• Food Engineering Progress
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• v.14 no.3
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• pp.202-207
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• 2010

Low-pressure plasmas (LPPs) were generated with different gases such as air, oxygen and nitrogen, and their inactivation effects against Escherichia coli were compared in order to evaluate the potential as a non-thermal microbial disinfection technology. Homogeneous plasmas were generated under low pressure below 1 Torr at gas flow rate of 350 mL/min regardless the types of gases. Temperature increases by LPPs were not detrimental showing less than ${10^{\circ}C}$ and ${25^{\circ}C}$ increases after 5 and 10 min treatments, respectively. The smallest temperature increase was observed with air LPP, and followed by oxygen and nitrogen LPPs. More than 5 log reduction in E. coli was achieved by 5 min LPP treatment but the destruction effect was retarded afterward. The LPP inactivation was represented by a iphasic first order reaction kinetics. The highest inactivation rate constant was achieved in air LPP and followed by oxygen and nitrogen LPPs. The small D-values of the LPP also supported its potentialities as a non-thermal food surface disinfection technology in addition to the substantial microbial reduction of more than 5 logs.

• Advances in concrete construction
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• v.6 no.6
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• pp.561-583
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• 2018

A variety of polycarboxylate ether (PCE)-based superplasticizers are commercially available. Their influence on the rheological retention and slump loss in respect of concrete differ considerably. Fluidity and slump loss are the cardinal features responsible for the quality of concrete. These are related to the dispersion of cement particles and the hydration process which are greatly influenced by type of polycarboxylate ether (PCE)-based superplasticizers. On the backdrop of relatively less studies in the context of rheological retention of high strength self-consolidating concrete (HS-SCC), the experimental investigations were carried out aiming at quantifying the effect of the six different PCE polymers (PCE 1-6) on the rheological retention of HS-SCC mixes containing two types of Ordinary Portland Cements (OPC) and unwashed crushed sand as the fine aggregate. The tests that were carried out included $T_{500}$, V-Funnel, yield stress and viscosity retention tests. The supplementary cementitious materials such as fly ash (FA) and micro-silica (MS) were also used in ternary blend keeping the mix paste volume and flow of concrete constant. Low water to binder ratio was used. The results reveal that not only the PCEs of different polymer groups behave differently, but even the PCEs of same polymer groups also behave differently. The study also indicates that the HS-SCC mixes containing PCE 6 and PCE 5 performed better as compared to the mixes containing PCE 1, PCE 2, PCE 3 and PCE 4 in respect of all the rheological tests. The PCE 6 is a new class of chemical admixtures known as Polyaryl Ether (PAE) developed by BASF to provide better rheological properties in even in HS-SCC mixes at low water to binder mix. In the present study, the PCE 6, is found to help not only in reduction in the plastic viscosity and yield stress, but also provide good rheological retention over the period of 180 minutes. Further, the early compressive strength properties (one day compressive strength) highly depend on the type of PCE polymer. The side chain length of PCE polymer and the fineness of the cement considerably affect the early strength gain.

• Journal of Ocean Engineering and Technology
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• v.33 no.2
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• pp.116-122
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• 2019

Among the various wave power systems, Salter's duck (rotor) is one of the most effective wave absorbers for extracting wave energy. The rotor shape is designed such that the front part faces the direction of the incident wave, which forces it to bob up and down due to wave-induced water particle motion, whereas the rear part, which is mostly circular in shape, reflects no waves. The asymmetric geometric shape of the duck makes it absorb energy efficiently. In the present study, the rotor was investigated using WAMIT (a program based on the linear potential flow theory in three-dimensional diffraction/radiation analyses) in the frequency domain and verified using OrcaFlex (design and analysis program of marine system) in the time domain. Then, an experimental investigation was conducted to assess the performance of the rotor motion based on the model scale in a two-dimensional (2D) wave tank. Initially, a free decay test (FDT) was carried out to obtain the viscous damping coefficient. The pitch response was extracted from the experimental time series in a periodic regular wave for two different wave heights (1 cm and 3 cm). In addition, the viscous damping coefficient was calculated from the FDT result and fluid forces, obtained from WAMIT, are incorporated into the final response of the rotor. Finally, a comparative study based on experimental and numerical results (WAMIT & OrcaFlex) was performed to confirm the performance reliability of the designed rotor.

• Structural Engineering and Mechanics
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• v.70 no.4
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• pp.479-497
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• 2019

In the current code design, the use of a uniform internal pressure coefficient of cooling towers as internal suction cannot reflect the 3D characteristics of flow field inside the tower body with different ventilation rate of shutters. Moreover, extreme weather such as heavy rain also has a direct impact on aerodynamic force on the internal surface and changes the turbulence effect of pulsating wind. In this study, the world's tallest cooling tower under construction, which stands 210m, is taken as the research object. The algorithm for two-way coupling between wind and rain is adopted. Simulation of wind field and raindrops is performed iteratively using continuous phase and discrete phase models, respectively, under the general principles of computational fluid dynamics (CFD). Firstly, the rule of influence of 9 combinations of wind speed and rainfall intensity on the volume of wind-driven rain, additional action force of raindrops and equivalent internal pressure coefficient of the tower body is analyzed. The combination of wind velocity and rainfall intensity that is most unfavorable to the cooling tower in terms of distribution of internal pressure coefficient is identified. On this basis, the wind/rain loads, distribution of aerodynamic force and working mechanism of internal pressures of the cooling tower under the most unfavorable working condition are compared between the four ventilation rates of shutters (0%, 15%, 30% and 100%). The results show that the amount of raindrops captured by the internal surface of the tower decreases as the wind velocity increases, and increases along with the rainfall intensity and ventilation rate of the shutters. The maximum value of rain-induced pressure coefficient is 0.013. The research findings lay the basis for determining the precise values of internal surface loads of cooling tower under extreme weather conditions.

• Journal of Microbiology and Biotechnology
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• v.31 no.8
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• pp.1069-1078
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• 2021

Sevoflurane postconditioning (SPostC) has been proved effective in cardioprotection against myocardial ischemia/reperfusion injury. It was also reported that heat shock protein 70 (HSP70) could be induced by sevoflurane, which played a crucial role in hypoxic/reoxygenation (HR) injury of cardiomyocytes. However, the mechanism by which sevoflurane protects cardiomyocytes via HSP70 is still not understood. Here, we aimed to investigate the related mechanisms of SPostC inducing HSP70 expression to reduce the HR injury of cardiomyocytes. After the HR cardiomyocytes model was established, the cells transfected with siRNA for HSP70 (siHSP70) or not were treated with sevoflurane during reoxygenation. The lactate dehydrogenase (LDH) level was detected by colorimetry while cell viability and apoptosis were detected by MTT and flow cytometry. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blotting were used to detect HSP70, apoptosis-, cell cycle-associated factors, iNOS, and Cox-2 expressions. Enzyme-linked immuno sorbent assay (ELISA) was used to measure malondialdehyde (MDA) and superoxide dismutase (SOD). SPostC decreased apoptosis, cell injury, oxidative stress and inflammation and increased viability of HR-induced cardiomyocytes. In addition, SPostC downregulated Bax and cleaved caspase-3 levels, while SPostC upregulated Bcl-2, CDK-4, Cyclin D1, and HSP70 levels. SiHSP70 had the opposite effect that SPostC had on HR-induced cardiomyocytes. Moreover, siHSP70 further reversed the effect of SPostC on apoptosis, cell injury, oxidative stress, inflammation, viability and the expressions of HSP70, apoptosis-, and cell cycle-associated factors in HR-induced cardiomyocytes. In conclusion, this study demonstrates that SPostC can reduce the HR injury of cardiomyocytes by inducing HSP70 expression.

• International Journal of Naval Architecture and Ocean Engineering
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• v.12 no.1
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• pp.71-84
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• 2020

In order to improve the performance of marine centrifugal pump, a centrifugal pump whose specific speed is 66.7 was selected for the research. Outlet diameter D2, outlet width b2, blade outlet angle β2, blade wrap φ and blade number z of the impeller were chosen as the variables. The maximum weighted average efficiency and the minimum vibration intensity at the base were calculated as objectives. Based on the Latin Hypercube method, the impeller was numerically optimized. The numerical results show that after optimization, the amplitudes of pressure fluctuation on the main frequency at different monitoring points decrease in varying degrees. The radial force on impeller decreases obviously under off-design flow rates and is more symmetrical during the operation of the pump. The variation of the axial force is relatively small, which has no obvious relationship with the rotating angle of the impeller. The energy performance and vibration experiment was performed for verifying. The test results show that the weighted average efficiency under 0.8Qd, 1.0Qd and 1.2Qd increases by 4.3% after optimization. The maximal vibration intensity at M1-M4 on the pump base reduced from 0.36 mm/s to 0.25 mm/s, decreasing by 30.5%. In addition, the vibration velocities of bracket in pump side and outlet flange also have significant reductions.

• International Journal of Naval Architecture and Ocean Engineering
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• v.12 no.1
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• pp.399-413
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• 2020

The numerical simulation of liquid sloshing in the three-dimensional tanks under horizontal excitation and roll excitation was carried out, and the inhibition effect of different baffles on the sloshing phenomenon was investigated. The numerical calculations were carried out by the nonlinear Boundary Element Method (BEM) with Green's theorem based on the potential flow, which was conducted with the governing equation corresponding to the boundaries of each region. The validity of the method was verified by comparing with experimental values and published literatures. The horizontal baffle, the vertical baffle and the T-shaped baffle in the sloshing tanks were investigated respectively, and the baffles' position, dimension and the liquid depth were provided and discussed in detail. It is drawn that the baffle shape plays a non-negligible role in the tank sloshing. The vertical baffle is a more effective way to reduce the sloshing amplitude when the tank is under a horizontal harmonic excitation while the horizontal baffle is a more effective way when the tank is under a roll excitation. The amplitude of free surface elevation at right tank wall decreases with the increasing of the horizontal baffle length and the vertical baffle height. Although the T-shaped baffle has the best suppression effect on tank sloshing under horizontal excitation, it has limited suppression effect under roll excitation and will complicate the sloshing phenomenon when changing baffle height.