• Journal of Ginseng Research
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• 제45권5호
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• pp.539-545
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• 2021

Background: Red ginseng polysaccharides (RGPs) have been acknowledged for their outstanding immunomodulation and anti-tumor activities. However, their studies are still limited by the complexity of their structural features, the absence of purification and enrichment methods, and the rarity of the analytical instruments that apply to the analysis of such macromolecules. Thus, this study is an attempt to establish a new mass spectrometry (MS)-based analysis procedure for RGPs. Methods: Saponin pre-excluded powder of RG (RG-SPEP, 10 mg) was treated with 200 µL of distilled water and centrifuged for 5 h at 1000 rpm and 85 ℃. Ethanol-based precipitation and centrifugation were applied to obtain RGPs from the heated extracts. Further, endo-carbohydrase treatments were performed to produce specific saccharide fragments. Solid-phase extraction (SPE) processes were implemented to purify and enrich the enzyme-treated RGPs, while matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF) MS was employed for the partial structural analysis of the obtained RGPs. Results: Utilizing cellulase, porous graphitized carbon (PGC), hydrophilic interaction chromatography (HILIC), and MALDI-TOF/TOF MS, the neutral and acidic RGPs were qualitatively analyzed. Hex_n and Hex_n-18 (cellulose analogs) were determined to be novel neutral RGPs. Additionally, the [Unknown + Hex_n] species were also determined as new acidic RGPs. Furthermore, HexA_n (H) was determined as another form of the acidic RGPs. Conclusion: Compared to the previous methods of analysis, these unprecedented applications of HILIC-SPE and MALDI-TOF/TOF MS to analyze RGPs proved to be fairly effective for fractionating and detecting neutral and acidic components. This new procedure exhibits great potential as a specific tool for searching and determining various polysaccharides in many herbal medicines.

• 한국응용곤충학회지
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• 제39권3호
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• pp.153-156
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• 2000

잎굴파리를 예찰할 때 이용하는 황색 끈끈이 트랩의 최적 설치 높이를 밝히기 위하여 트랩을 3단계의 높이로 설치하여 4회에 걸쳐 높이별 부착수를 조사하였다. 거베라에서 트랩의 설치 높이는 작물과 같은 높이로 설치했을 때가 그 위 30cm, 60cm에 설치했을 때보다 잎굴파리의 유인량이 많았다. 시간대별 잎굴파리의 행동향상을 파악하기 위하여 황색 끈끈이 트랩을 작물높이에 설치한 후 1시간과 2시간 간격으로 일주활동을 조사하였다. 5월 조사에서 08~10시와 14~18시에 두 번의 피크가 나타났으며 7월 조사에서는 08시까지 잡힌 잎굴파리가 가장 많았고, 10월 조사에서는 12~14시에 가장 많이 잡혔다 잎굴파리의 배행활동에는 온도뿐만 아니라 일사강도 등 다른 환경요인도 작용하는 것으로 조사되었다. 또한, 황색 끈끈이트랩에는 암컷보다 수컷이 트랩의 높이나 시간에 관계없이 더 잘 유인되었다.

• 한국주거학회논문집
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• 제14권5호
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• pp.117-128
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• 2003

The purpose of this study is to investigate the effect of functional ability on relocation among the elderly. Longitudinal data from the Asset and Health Dynamics among the Oldest Old (1993, 1995) were analyzed for 6,225 respondents aged 70 or older. Among functional ability measures, difficulties in advanced activities of daily living (i. e., making telephone calls, taking medications, and managing money), lower body activities (i. e., walking several blocks, climbing one flight of stairs, pulling or pushing large objects, and carrying over 10 pounds), and household activities of daily living (i. e., preparing hot meals and shopping for groceries) were the predictors of increased residential move. Difficulties in basic activities of daily living (i. e., bathing, dressing, getting in and out, and using the toilet) were strongly related to increased entrance into an institutional care facility. Efforts to enhance the independent living of the elderly in the community setting should target the compensation of these functional difficulties.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제5권2호
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• pp.37-42
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• 2024

This study used a GPS tracker to determine characteristics of wintering habitat use by black-tailed gulls on the west coast of Korea. Results of this study showed a southward movement in December and a northward movement in March. The wintering habitat was confirmed to include the southwest coastal island area (Group 1), the inland coastal area (Group 2), and China (Group 3). The wintering home range was the narrowest in October and the widest in December. Home range of Group 1 was increased from November, showing the maximum in December, decreased in February, and then tended to increase again in March. Group 2 showed the widest home range in November and a narrow range of home range from December. Home range of Group 3 showed a mixed pattern of group 1 and group 2. The flight rate was the lowest in January and the highest in March. Day and night flight rates showed significant differences by month. This study identified wintering characteristics of the West Coast population of black-tailed gulls on the West Coast, including their wintering areas, migration periods, and use patterns. These wintering characteristics of black-tailed gull in the west coast area are thought to be affected by environmental factors (such as food resources and fishing activities) and geographical characteristics of the West Coast where many islands are distributed.

• 한국군사과학기술학회지
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• 제9권3호
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• pp.20-24
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• 2006

In this paper, our research efforts and activities for displaying flight simulation graphics on multi-monitors connected to a Linux cluster is introduced. In this pilot system graphics rendering as well as view calculation including LOD implementation for each monitor is peformed on each sub-node computer connected to the monitor rather than using an expensive main server. The Linux cluster is constructed by combining general-purpose desktop computers, and MPI library Is used for communication between sub-nodes. It could be concluded from our experience that it is possible to construct a massive multi-monitor display system by adding to the cluster as many sub-node computers and monitors as possible with economic efficiency.

• Journal of Ginseng Research
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• 제43권4호
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• pp.539-549
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• 2019

Background: 20(S)-Protopanaxadiol (PPD), the aglycone part of 20(S)-protopanaxadiol ginsenosides, possesses antidepressant activity among many other pharmacological activities. It is currently undergoing clinical trial in China as an antidepressant. Methods: In this study, an ultra-performance liquid chromatography coupled with triple quadrupole time-of-flight mass tandem mass spectrometry method was established to identify the metabolites of PPD in human plasma and urine following oral administration in phase IIa clinical trial. Results: A total of 40 metabolites in human plasma and urine were identified using this method. Four metabolites identified were isolated from rat feces, and two of them were analyzed by NMR to elucidate the exact structures. The structures of isolated compounds were confirmed as (20S,24S)-epoxydammarane-12,23,25-triol-3-one and (20S,24S)-epoxydammarane-3,12,23,25-tetrol. Both compounds were found as metabolites in human for the first time. Upon comparing our findings with the findings of the in vitro study of PPD metabolism in human liver microsomes and human hepatocytes, metabolites with m/z 475.3783 and phase II metabolites were not found in our study whereas metabolites with m/z 505.3530, 523.3641, and 525.3788 were exclusively detected in our experiments. Conclusion: The metabolites identified using ultra-performance liquid chromatography coupled with triple quadrupole time-of-flight mass spectrometry in our study were mostly hydroxylated metabolites. This indicated that PPD was metabolized in human body mainly through phase I hepatic metabolism. The main metabolites are in 20,24-oxide form with multiple hydroxylation sites. Finally, the metabolic pathways of PPD in vivo (human) were proposed based on structural analysis.

• 한국경영과학회:학술대회논문집
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• 한국경영과학회 2007년도 추계학술대회 및 정기총회
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• pp.27-37
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• 2007

Many problems related to airline business belong to large-scale optimization problems, so that it is expected that the state-of-art optimization techniques are widely applied to making the airline operation effective and competitive. This paper introduces the concepts and mathematical models of various optimization problems in airline system. Airlines involve many activities that utilize airline resources such as aircrafts and crews to make profit. We view the airline activities in the planning and operational aspects. In the planning viewpoint, we discuss the flight schedule design problem that impacts on passenger demand directly. For aircraft and crews, we deal with fleet assignment, aircraft routing, crew pairing optimization, and crew assignment problem. In the operational viewpoint, we concern schedule recovery problems for aircrafts and crew using the method of reassigning available resources when airlines face with the unexpected situations.

• 한국환경과학회지
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• 제29권8호
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• pp.809-818
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• 2020

To conserve the population of a hesperiid butterfly, Leptalina unicolor, inhabiting the protected areas of Jaeyaksan, we provide ecological information on their behavior and propose habitat conservation measures, such as the creation of alternative habitats based on comprehensive information. The behavioral study used a method of re-capture after releasing the butterfly with enamel marks on the wings. Adult behaviors were shown in four patterns: flying, settling on a plant, nectar absorption, and water absorption. Both males and females had the highest proportion of flight movement overall; however, males had a slightly higher proportion of flight movement. As for duration, females spent more time settling on plants to select spawning sites, and males seemed to take a longer time for water absorption activities, to absorb minerals needed to form the spermatophore. The average travel distance of butterflies was 27.5 m for females and 46.7 m for males, with daily activity ranges from 11.2 m to 43.8 m, and 21.4 to 57.6 m, respectively. The most important condition to preserve the habitat of Leptalina unicolor is to maintain the community of Miscanthus sinensis, a food plant. Additionally, because this butterfly has a high rate of water absorption activities, wetlands should be maintained.

• 대한의생명과학회지
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• 제6권4호
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• pp.269-279
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• 2000

격렬한 운동유형으로 분류되는 비행이 습성화 되어 있는 조류를 대상으로, 인위적으로 비행을 제한시켰을 떼 나타나는 심근의 퇴행성 변화를 살펴봄으로써, 운동량 감소가 심근에 미치는 영향에 대하여 검토하고자 하였다. 서식환경의 제한을 위해 자연상태에서 서식하는 비둘기를 1 $m^3$ 공간에 2달 동안 서식시킨 군 (confined type)과 자연상태의 군 (wild type)으로 나누어 심근의 변화양상을 미세구조적, 세포화학적 방법 및 입체해석학적 방법으로 분석하였다. 체중은 confined type에서 증가되어 상대적으로 심장의 무게는 감소되었고, 흉근의 무게는 6.5% 감소 (p<0.05) 되었다. Confined type은 wild type과 비교해 볼 때, 미세구조적으로 더 적은 빈도의 심근의 파동형 변화와 지방소적의 침적이 관찰되었고, 근절의 길이는 길게 관찰되었다. Cytochrome oxidase의 활성을 알아보기 위해 실시한 세포 화학적 반응 결과, confined type에서 활성이 15% 감소되었다. 또한 입체해석학적 분석 결과, confined type의 관원섬유 체적밀도는 높게 나타났으나, 근형질내세망의 체적밀도와 사립체 내막의 표면밀도는 각각 23%, 25% 낮게 (p<0.05, p<0.01) 나타났다. 사립체의 체적밀도는 confined type에서 낮게 나타난 반면, 수적 밀도는 wild type과 비교하여 17% 높게 (p<0.05) 나타났다. 이러한 결과를 종합해보면 비행이라는 고에너지를 요구하는 격렬한 운동인 비행이 습성화 되어 있는 비둘기에서 비행을 제한시키면, 심근은 운동량의 감소에 따른 퇴행성 변화를 겪게 되므로, 운동의 지속성 여부는 심근기능유지에 매우 중요한 요소로 판단된다.

• 대한인간공학회지
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• 제17권3호
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• pp.23-39
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• 1998

Several recent aircraft accidents occurred due to goal conflicts between human and machine actors. To facilitate the management of the cockpit activities considering these observations. a computational aid. the Agenda Manager (AM) has been developed for use in simulated cockpit environments. It is important to know pilot intentions performing cockpit operations accurately to improve AM performance. Without accurate knowledge of pilot goals or intentions, the information from AM may lead to the wrong direction to the pilot who is using the information. To provide a reliable flight simulation environment regarding goal conflicts. a pilot goal communication method (GCM) was developed to facilitate accurate recognition of pilot goals. Embedded within AM, the GCM was used to recognize pilot goals and to declare them to the AM. Two approaches to the recognition of pilots goals were considered: (1) The use of an Automatic Speech Recognition (ASR) system to recognize overtly or explicitly declared pilot goals. and (2) inference of covertly or implicitly declared pilot goals via the use of an intent inferencing mechanism. The integrated mode of these two methods could overcome the covert goal mis-understanding by use of overt GCM. And also could it overcome workload concern with overt mode by the use of covert GCM. Through simulated flight environment experimentation with real pilot subjects, the proposed GCM has demonstrated its capability to recognize pilot intentions with a certain degree of accuracy and to handle incorrectly declared goals. and was validated in terms of subjective workload and pilot flight control performance. The GCM communicating pilot goals were implemented within the AM to provide a rich environment for the study of human-machine interactions in the supervisory control of complex dynamic systems.