• Title/Summary/Keyword: Fleshy shrimp

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Comparison of maturation and secondary spawning of fleshy shrimp Penaeus chinensis fed different kinds of diets

  • Youngguk Jin;Yu-Hyeon Jeon;Sukyoung Kim;Eunha Jeong;Su-Kyoung Kim
    • Fisheries and Aquatic Sciences
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    • v.25 no.12
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    • pp.619-625
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    • 2022
  • The study carried out a 15-day feeding trial of the fleshy shrimp broodstock that have just finished their first spawn using five different maturation feed types (three types of manufactured feed, fresh feed, and commercial feed) to induce multiple spawning in the shrimp and then analyzed and compared hemolymph levels of total protein, total lipid, hemocyanin, glucose, and cholesterol as well as ovary histology between the treatments to identify the potential of the experimental diets to be utilized as maturation feed. The study found that the manufactured diets of polychaetes + clam and polychaetes + clam + squid resulted in significantly higher levels of lipid and protein in shrimp hemolymph suggesting an indicator of multiple spawning in the fleshy shrimp. The other manufactured diet of polychaets + squid led to the highest 2nd spawning with significantly lower levels of lipid, protein, and cholesterol in shrimp hemolymph. The fresh feed treatment showed similar hematological as well as histological responses to the manufactured diet of polychaetes + squid, but with a significantly lower spawning volume compared to the other treatments. The study demonstrated that the manufactured diets could replace fresh feed as maturation feed and attain a higher shrimp maturation than the commercial feed.

Morphology and Ultrastructure on the Gill of the Fleshy Shrimp, Penaeus chinensis (Decapoda: Penaeidae) (대하(Penaeus chinensis) 아가미의 형태 및 미세구조)

  • Lee, Jung-Sick;Kang, Ju-Chan;Jeong, Seon-Young
    • Applied Microscopy
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    • v.30 no.3
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    • pp.311-319
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    • 2000
  • The gill morphology and ultrastructure of the fleshy shrimp, Penaeus chinensis were investigated by light and electron microscopy. Fleshy shrimp has dendrobranchiate gills. Gill has a longitudinal septum dividing them into afferent and efferent channel. Each gill lamella is covered by multi-layered thin cuticle of different electron density. The lamella basal cell is squamous and contains cytoplasm of electron dense. Simple epithelial layer consists of squamous epithelium contained large nucleus. The lamella pillar structures are characterized by the axial microtubules and lateral membrane interdigitations Secretory cells of AB-PAS negative are multicellular gland. In active gland each cell boundary is not apparent and the cytoplasm contains smooth endoplasmic reticula, mitochondria, membrane-bounded secretory vesicles of low electron density and granular resettes. In inactive gland each cell boundary is apparent and the cytoplasm is occupied with numerous small granules of electron dense. The well-developed rough endoplasmic reticula and Golgi apparatus are observed in the unicellular gland of alcian blue positive.

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Optimum Level of Protein and the Possibility of Replacement of Fish Meal by Soybean Meal in Feeds for Fleshy Shrimp, Penaeus chinensis (대하 (Penaeus chinensis)의 단백질요구량과 단백질원으로서 대두박의 이용)

  • KIM Hyun Jun;HUR Sung Bum
    • Journal of Aquaculture
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    • v.6 no.3
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    • pp.173-186
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    • 1993
  • In order to substitute soybean meal for fish meal, the protein requirement of fleshy shrimp was examined and then the effect of soybean meal supplemented with cuttlefish oil was studied. Different contents of protein in prepared diet had significant effects on the survival rate and growth of fleshy shrimp (P<0.05). With regard to protein content ranging from $25\%\;to\;50\%,\;40\%$ protein showed the best survival rate and fastest growth. The percent survival rates of fleshy shrimp fed diets containing protein levels of 25, 30, 35, 40, 45 and $50(\%)$ were $74.0^d,\; 82.0^{cd},\;91.0^b,\;97.0^a,\;93.0^{ab},\;and\;88.0^{bc}\%(P< 0.05).$ The optimum protein requirements for postlarvae (13-84 mg) and juvenile (0.9-8.1g) were calculated by the broken line method as $40.4\%\;and\;39.9\%$. When soybean meal was substituted $(up\;to\;76\%)$ for fish meal in the prepared diet, the performance' gradually suffered with the increase of soybean meal content, but when cuttlefish oil was added to soybean meal, survival rates were improved with $2.5\%$ cuttlefish oil supplement.

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Effects of Serotonin, Ecdysone and HCG on Ovarian Maturation of the Fleshy Prawn, Fenneropenaeus chinensis (대하(Fenneropenaeus chinensis)의 난소 성숙에 대한 Serotonin, Ecdysone 그리고 HCG의 효과)

  • Lee, Jae-Yong;Kim, Dae-Jung;Kim, Dae-Hyun;Kim, Myung-Hee;Kim, Byung-Ki;Lee, Bok-Kyu;Han, Chang-Hee
    • Development and Reproduction
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    • v.13 no.4
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    • pp.341-351
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    • 2009
  • The effects of serotonin (5-hydroxytryptamine, 5-HT), ecdysone (20-hydroxyecdysone, 20-E) and HCG (human chorionic gonadotropin) on the ovarian maturation of the fleshy shrimp, Fenneropenaeus chinensis were investigated. Adult female fleshy shrimps were injected with 5-HT ($5{\mu}g$/g body weight (BW) or $20{\mu}g$/g BW), 20-E ($5{\mu}g$/g BW, $10{\mu}g$/g BW or $20{\mu}g$/g BW) or HCG (5 IU/g BW or 10 IU/g BW) three times at 5-day intervals and were sacrificed 10 days after the last injection. The effects were compared against an intact control group and sham control group which received the injection of sterile saline solution as a vehicle. 20-E and HCG showed no effectiveness on ovarian maturation in fleshy shrimps. However flesh shrimp given 5-HT of $20{\mu}g$/g BW showed significant increase in gonadosomatic index (GSI) in early spring. The results suggest that treatment of 5-HT of $20{\mu}g$/g BW or more is useful approach for the nursery production of the fleshy shrimp, Fenneropenaeus chinensis aquaculture.

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Development of a Single Tangle Net for the Brown Shrimp by Observation of Entanglement Behaviour

  • Kim Yong-Hae
    • Fisheries and Aquatic Sciences
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    • v.6 no.1
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    • pp.34-40
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    • 2003
  • Three panel trammel nets were made illegal for the brown shrimp (Penaeus japonicus) or fishes by Korean fisheries law while trammel nets for fleshy prawn in the West sea remained legal. In this study a single panel tangle net with vertical loop lines rigged between the float line and sinker line was specially designed to catch brown shrimp. This net was developed for the first time after observation of the brown shrimp behaviour when reacting to a net in an observation tank. In field experiments these single tangle nets were compared with the traditional trammel nets in the coastal waters of the Keoje area. The mean number of the brown shrimp for 53 fishing operations was 1.13 per unit panel of the single tangle nets when fitted with the vertical loop lines. This was $84\%$ of the mean catch of 1.36 achieved with the trammel nets. These results of fishing experiments using single tangle nets in the field revealed a high fishing efficiency for the brown shrimp and showed little difference from trammel nets. The size of the brown shrimp or number of by-catch was not different between single tangle nets and trammel nets.

Expression of c-Type Lysozyme from the Fleshy Shrimp Fenneropenaeus chinensis Is Upregulated Following Vibrio anguillarum and Lipopolysaccharide Injection

  • Qiao, Guo;Kim, Su-Kyoung;Cho, Yeong-Rok;Kim, Sukyoung;Jang, In-Kwon
    • Fisheries and Aquatic Sciences
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    • v.16 no.4
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    • pp.267-272
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    • 2013
  • Chicken-type lysozyme (c-lysozyme) is present in shrimp and is active against some bacteria. To further understand the regulation of c-lysozyme in the fleshy shrimp Fenneropenaeus chinensis, we determined the tissue-specific gene expression of c-lysozyme and the time-course of mRNA expression in response to Vibrio anguillarum and lipopolysaccharide (LPS) injection by quantitative reverse real-time polymerase chain reaction. The results showed that c-lysozyme was expressed in all tissues tested, including gill, eyestalk, eye, hemocytes, hepatopancreas, intestine, heart, and pleopod. It was most highly expressed in the intestine followed by the eyestalk, gill, hemocytes and hepatopancreas. The mRNA expression level began to decline in a short time after V. anguillarum challenge and was then upregulated by two fold or more at 24 h post injection (hpi) compared to that at 0 h. Expression was suppressed shortly after LPS injection and began to increase with higher levels of 5.8-, 5.2- and 8.4-fold at 24, 48, and 72 hpi, respectively. Higher expression was sustained and showed a gradual increasing trend until the end of the experiment (72 hpi). These results increase our understanding of the regulation of defense mechanisms and facilitate an evaluation of the effects of probiotics or immunostimulants in shrimp culture.

Effects of Temperature and Salinity on Hatching Rate of Fertilized Egg in Fleshy Shrimp, Penaeus chinensis (대하(Penaeus chinensis) 수정난의 부화에 미치는 수온과 염분의 영향)

  • HUR Sung Bum;KIM Hyun Jun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.1
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    • pp.1-8
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    • 1996
  • The effects of temperature and salinity on hatching rate of fertilized egg were examined to develop the method for the efficient seed production in fleshy shrimp, Penaeus chinensis. At the temperature ranging from $20^{\circ}C\;to\;24^{\circ}C$, the hatching rate increased with temperature and the highest hatching rate $(95.5\%)$ occurred at $24^{\circ}C$. But, with the temperature above $26^{\circ}C$, the hatching rate decreased suddenly, and at $30^{\circ}C$, the egg did not hatch at all. The hatching rate increased with salinity within the range of $20\%_{\circ}\;to\;31\%_{\circ}$ and the highest hatching rate $(95.5\%)$ occurred at $31\%_{\circ}$. However, with the salinity above $31\%_{\circ}$, the rate decreased abruptly, and it was only $25.5\%\;at\;40\%_{\circ}$. The hatching speed of egg was the fastest at $24^{\circ}C$. The hatching rate at $24^{\circ}C$ with the salinity of $31\%_{\circ}\;was\;30.5\%$ within 18hrs of the hatching experiment and it attained to $95.5\%$ within 48hrs of the hatching experiment. At $24^{\circ}C$, the hatching rate with the salinity of $30\~31\%_{\circ}$ is 1,2 times higher than that of egg with the salinity of $33\~35\%_{\circ}$.

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The Study of Genetic Diversity and Population Structure of the Korean Fleshy Shrimp, Fenneropenaeus chinensis, Using Newly Developed Microsatellite Markers (새로 개발한 미세위성체 마커를 이용한 한국 대하의 유전다양성 및 집단구조)

  • Shin, Eun-Ha;Kong, Hee Jeong;Nam, Bo-Hye;Kim, Young-Ok;Kim, Bong-Seok;Kim, Dong-Gyun;An, Cheul Min;Jung, Hyungtaek;Kim, Woo-Jin
    • Journal of Life Science
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    • v.25 no.12
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    • pp.1347-1353
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    • 2015
  • The fleshy shrimp, Fenneropenaeus chinensis, is the family of Penaeidae and one of the most economically important marine culture species in Korea. However, its genetic characteristics have never been studied. In this study, a total of 240 wild F. chinensis individuals were collected from four locations as follows: Narodo (NRD, n = 60), Beopseongpo (BSP, n = 60), Chaesukpo (CSP, n = 60), and Cheonsuman (CSM, n = 60). Genetic variability and the relationships among four wild F. chinensis populations were analyzed using 13 newly developed microsatellite loci. Relatively high levels of genetic variability (mean allelic richness = 16.87; mean heterozygosity = 0.845) were found among localities. Among the 52 population loci, 13 showed significant deviation from the Hardy–Weinberg equilibrium. Neighbor-joining, principal coordinate, and molecular variance analyses revealed the presence of three subpopulations (NRD, CSM, BSP and CSP), which was consistent with clustering based on genetic distance. The mean observed heterozygosity values of the NRD, CSM, BSP, and CSP populations were 0.724, 0.821, 0.814, and 0.785 over all loci, respectively. These genetic variability and differentiation results of the four wild populations can be applied for future genetic improvement using selective breeding and to design suitable management guidelines for Korean F. chinensis culture.

Development of Sandwich ELISA for the Detection of Shrimp in Processed Foods (가공식품 중 새우의 검출을 위한 샌드위치 ELISA의 개발)

  • Do, Jeong-Ryong;Back, Su-Yeon;Shon, Dong-Hwa
    • Korean Journal of Food Science and Technology
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    • v.46 no.5
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    • pp.538-543
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    • 2014
  • A sandwich enzyme-linked immunosorbent assay method (sELISA) for detecting the presence of shrimp in processed foods was developed using rabbit polyclonal antibodies against tropomyosin produced by black tiger prawns (shrimp). Based on the standard curve derived using this method, the detection range of shrimp was determined to be $1-100{\mu}g/mL$. The cross-reactivity of these antibodies toward black tiger prawns, fleshy prawns, cocktail prawns, lobster, and blue crab was 100, 73, 155, 18, and 0%, respectively. When shrimp was heated for 10 min, the mean assay recovery of tropomyosin was 121-221% at $70-100^{\circ}C$ and 7.8% at $121^{\circ}C$. When shrimp was added to cream soup, weaning food, sausage, fish paste, and sauce, the mean assay recovery was 397, 639, 168, 234, and 0%, respectively. In sample tests involving 14 commercial items, the coincidence ratio of assay results and reference was 79%.

Species Identification and Labeling Compliance Monitoring of Commercial Shrimp Products Sold in Online Markets of South Korea (국내 온라인 유통 새우 제품의 종판별 및 표시사항 모니터링 연구)

  • Kun Hee Kim;Ji Young Lee;Tae Sun Kang
    • Journal of Food Hygiene and Safety
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    • v.38 no.6
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    • pp.496-507
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    • 2023
  • This study investigated species identification and labeling compliance of 48 shrimp products sold in the Korean online markets. Species identification was conducted using the standard DNA barcoding method, using the cytochrome c oxidase subunit I gene. The obtained sequences were compared with those deposited in the NCBI GenBank and BOLD Systems databases. Additionally, phylogenetic analysis was performed to further verify the identified shrimp species. Consequently, 16 shrimp species were identified, including Penaeus vannamei, Pandalus borealis, Palaemon gravieri, Leptochela gracilis, Penaeus monodon, Pleoticus muelleri, Metapenaeopsis dalei, Euphausia pacifica, Lebbeus groenlandicus, Trachypenaeus curvirostris, Argis lar, Metanephrops thomsoni, Metapenaeopsis barbata, Alpheus japonicus, Penaeus chinensis, and Mierspenaeopsis hardwickii. The most prevalent species was Penaeus vannamei, found in 45.8% of the analyzed products. A significant mislabeling rate of 72.9% was found; however, upon excluding generic names such as shrimp, the mislabeling rate reduced to 10.4%. The mislabeling rate was higher in highly-processed products (89.3%) compared with that in minimally-processed products (50%). No correlation was found between the country of origin and mislabeling rate. The results of this study provide crucial data for future monitoring of shrimp products and improving the labeling of shrimp species in Korea.