• Molecular & Cellular Toxicology
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• v.1 no.2
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• pp.87-91
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• 2005

Although 2D-PAGE has been widely used as the primary method for protein separation, difficulties in displaying proteins with an extreme values of isoelectric paint (pI), molecular size and hydrophobicity limit the technique. In addition, time consuming steps involving protein transfer and extraction from the gel-pieces can result in sample loss. Here, we describe a novel protein separation technique with capillary size-exclusion chromatography (CSEC) for rapid protein identification from human plasma. The method includes protein fractionation along with molecular size followed by in-solution tryptic digestion and peptide analysis through reversed phase liquid chromatography (RPLC) coupled to nanoflow electrospray-tandem mass spectrometry (ESI-MS/MS). Tryptic peptides are applied an a $100\;{\mu}m\;i.d.{\times}10mm$ length pre-column and then separated on a $75\;{\mu}m{\times}200mm$ analytical column at -100 nL/min flaw rate. Proteins were identified over the wide ranges of pI (3.7-12.3) when this technique was applied to the analysis of $1-2\;{\mu}L$ of human plasma. This gel-free system provides fast fractionation and may be considered a complementary technique to SDS-PAGE in proteomics.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.24 no.5 s.176
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• pp.1331-1342
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• 2000

In power generation systems a variety of structural components typically operate at high temperature and pressure. Therefore a life assessment methodology accounting for gradual creep fracture is increasingly needed for these components. The most critical defects in such structure are generally found in the form of semi-elliptical surface cracks in the welded tubular joints. Therefore the analysis of a semi-elliptical surface crack in a plate or a shell is an important problem in engineering fracture mechanics. On this background, via shell/line-spring finite element analyses of such surface cracks in the welded T and L joints under various loadings, we investigate J-integral along the crack front We first develop T and L joints auto mesh generation program providing ABAQUS input file composed of shell/line-spring finite elements. We then further develop a T and L joints life assessment program based on the experimental creep crack growth law and auto mesh generation program in a graphical user interface format Finally the remaining life of T and L joints for various analytical parameters are assessed using the developed life assessment program.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.33 no.11
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• pp.1187-1194
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• 2009

During the last decade, possibility of flaw occurrences has been rapidly increased world-widely as the increase of operating times of petro-chemical facilities. For instance, from a recent in-service inspection, three different sized surface cracks were detected in welding parts of a spherical oxygen holder in Korea. While API579 code provides corresponding engineering assessment procedures to determine crack driving forces, in the present work, numerical analyses are carried out for the cracked oxygen holder to investigate effects of complex geometry, analysis model and residual stress. With regard to the detailed finite element analysis, stress intensity factors are determined from both the full three-dimensional model and equivalent plate model. Also, as an alternative, stress intensity factors are calculated for equivalent plate model by employing the noted influence stress function technique. Finally, parametric structural integrity evaluation of the cracked oxygen holder is conducted in use of failure assessment diagram method, J/T method and DPFAD method. Effects of the geometry and so forth are examined and key findings from the simulations are fully discussed, which enables to determine practical safety margins of spherical components containing a defect.

• 한국전산유체공학회:학술대회논문집
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• 2011.05a
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• pp.124-128
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• 2011

The heat transfer rate in an oven is very important for the quality of cooking food. For a robust performance design in an electric oven, forced convection has been used rather than natural convection, in bake and convection mode. Forced convection heat trans for in a vented electric oven has been numerically evaluated using the commercial software FLUENT. CFD modeling of the electric oven involves three-dimensional, steady state, MRF fan model and DO radiation model. In this study, the electric oven cavity and fan modules are not simplified. Other research shows that the boundary condition can often lead to non-physical solutions, such as reverse flaw at the top vent. To remove this non-physical solution, control volume has been expanded at the nearby vent. This numerical analysis has been performed with dedicated experimental support. The results show that there is less than a 2.2% difference between the simulation and experimental data for the temperature profile of food. From this research we can use this oven simulation technique to make a better convection system in an electric oven.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.6 no.2
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• pp.751-765
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• 2012

Key establishment protocols are fundamental for establishing secure communication channels over public insecure networks. Security must be given the topmost priority in the design of a key establishment protocol. In this work, we provide a security analysis on two recent key establishment protocols: Harn and Lin's group key transfer protocol and Dutta and Barua's group key agreement protocol. Our analysis shows that both the Harn-Lin protocol and the Dutta-Barua protocol have a flaw in their design and can be easily attacked. The attack we mount on the Harn-Lin protocol is a replay attack whereby a malicious user can obtain the long-term secrets of any other users. The Dutta-Barua protocol is vulnerable to an unknown key-share attack. For each of the two protocols, we present how to eliminate their security vulnerabilities. We also improve Dutta and Barua's proof of security to make it valid against unknown key share attacks.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.9
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• pp.4588-4608
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• 2017

The measurement of information security levels is a very important but difficult task. So far, various measurement methods have studied the development of new indices. Note, however, that researches have focused on the problem of attaining a certain level but largely neglecting research focused on the issue of how different types of possible flaws in security controls affect each other and which flaws are more critical because of these effects. Furthermore, applying the same weight across the board to these flaws has made it difficult to identify the relative importance. In this paper, the interrelationships among security flaws that occurred in the security controls of K-ISMS were analyzed, and the relative impact of each security control was measured. Additionally, a case-control study was applied using empirical data to eliminate subjective bias as a shortcoming of expert surveys and comparative studies. The security controls were divided into 2 groups depending on whether or not a security flaw occurs. The experimental results show the impact relationship and the severity among security flaws. We expect these results to be applied as good reference indices when making decisions on the removal of security flaws in an enterprise.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2004.11a
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• pp.188-192
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• 2004

A Numerical analysis method is developed in order to compute the flaw and wave field for 2-dimensional floating body in the free roll motion with 3 degrees of freedom. Navier-Stokes and continuity equations are gaverning equations in tire present study. Finite Difference method is introduced to discretize the governing equation. The free surface is traced by the interface tracking method and the grid system is fitted to boundaries including free surface and body surface, which is moving in the flow field. The numerical scheme is based on Maker and Cell method. For the sake of validation of the numerical method, the computed roll decay factors according to tire midship section shapes are compared with measured results. The numerical results are discussed in order to understand the effect of midship section shape on roll motion.

• Journal of the Society of Naval Architects of Korea
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• v.28 no.2
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• pp.275-284
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• 1991

Fatigure crack growth from surface defects is one of the most important subjects for the evaluation and the assurance of safety in pressure vessels, piping systems, ship hulls and other various structures. This paper attempts to analysis some practical or general problems such as the estimation of crack growth life to penetrate the plate thickness, based on fatigure crack growth from a single surface flaw and the interaction of multiple flaws. An experiment on the coalescence of multiple undercuts was carried out under cyclic tension condition as a attempt to the analysis of multiple crack problems. It is noted that the fracture strength is characterized by the analogy to that in a single crack growth.

• Journal of the Korean Society for Nondestructive Testing
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• v.30 no.3
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• pp.261-270
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• 2010

This paper presents an analytical investigation for a baseline-free imaging of a defect in plate-like structures using the time-reversal of Lamb waves. We first consider the flexural wave (A0 mode) propagation in a plate containing a defect, and reception and time reversal process of the output signal at the receiver. The received output signal is then composed of two parts: a directly propagated wave and a scattered wave from the defect. The time reversal of these waves recovers the original input signal, and produces two additional side bands that contain the time-of-flight information on the defect location. One of the side band signals is then extracted as a pure defect signal. A defect localization image is then constructed from a beamforming technique based on the time-frequency analysis of the side band signal for each transducer pair in a network of sensors. The simulation results show that the proposed scheme enables the accurate, baseline-free detection of a defect, so that experimental studies are needed to verify the proposed method and to be applied to real structure.

• Proceedings of the KSR Conference
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• 2008.11b
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• pp.151-156
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• 2008

The after 1974 capital region electric railroad is opened to traffic Koraill and Seoul metro adopt each other different power supply method and they operate and they are come. AC 25[kv] method voltage the insulation becomes larger standing distance highly, the case tunnel section which will introduce in the underground segment becomes on a large scale and the cost of construction increases to the city center and the underground segment adoption of DC method was many. To recent times with R-bar (Rigid-bar) introductions change of tunnel section the introduction of AC method came to be easy without in the underground segment. Operates sees in the electric protection where the Seoul metro and the Korail are different each other and, from the dead section segment the interior electric light to put out lights the passenger brings about inconveniently and the civil appeal which demands a power supply method unification occurs, within the company interchange electric railroad brazier safety improvement and maintenance expense curtailment etc. the flaw is original proposal, is a condition which finishes a feasibility study service about the power supply method fringe land. Interchange analysis of the DC electric railway it leads from the present paper and it compares under analyzing being it could be reflected to also the route which is in the process of actual using boil the strong point and a weak point of DC and AC method of the establishment route constructive at the time of and