Journal of The Korean Society of Grassland and Forage Science
/
v.26
no.3
/
pp.171-176
/
2006
This experiment was carried out to breed the cold-tolerant variety of Italian ryegrass(Lolium multiflorum Lam.) in Grassland and Forages Division, National Livestock Research Institute, RDA, Suwon from 1987 to 2005. New variety, Kowinner as a tetraploid variety was dark green in leaf color and growth habit in autumn and early spring was semi-prostrate and medium, respectively Kowinner as a medium or late-heading variety was 19th May in heading date. Flag leaf length of Kowinner was 29 cm that was the same as control variety, Hwasan 101. Length of longest stem and plant length of Kowinner were 70 cm and 97 cm that was longer 3 cm than those of Hwasan 101, respectively. Stem width of Kowinner was thicker than that of Hwasan 101. Expecially, Kowinner was very good in regrowth. Cold tolerance of Kowinner was slightly weaker than that of Hwasan 101 and slightly stronger than that of Florida 80. Dry matter(DM) yield(12,884 kg/ha) of Kowinner was 8% more than that of Hwasan 101. In vitro dry matter digestibility and total digestible nutrient(TDN) of Kowinner were 76.2 and 63.3% that was 1.6 and 0.4% lower than those of Hwasan 101, respectively.
Choi Gi Jun;Rim Yong Woo;Sung Byung Ryul;Lim Young Chul;Kim Meing Jooung;Kim Ki-Yong;Park Geun Je;Park Nam Keon;Hong Youn Ki;Kim Sang Rok
Journal of The Korean Society of Grassland and Forage Science
/
v.25
no.4
/
pp.275-280
/
2005
This experiment was carried out to breed the cold-tolerant variety of Italian ryegrass (Lolium multiflorum Lam.) in Grassland and Forage Crops Division, National Livestock Research Institute, RDA, Suwon from 1997 to 2002. Hwasan 104 as a tetraploid variety was dark green in leaf color and had semi-prostrate and medium growth habit in late autumn and early spring, respectively. Hwasan 104 was 19th May in heading date as a late-heading variety. Especially, Hwasan 104 was broader in flag leaf and longer 7 cm in plant length, and thicker in stem width than those of control variety, Hwasan 101. Cold tolerance of Hwasan 104 was better than that of Hwasan 101 registered as a cold-tolerant variety. Dry matter (DM) yield of Hwasan 104 was $5\%$ more than DM 9,348 kg/ha of Hwasan 101. In vitro dry matter digestibility and total digestible nutrient of Hwasan 104 were 74.3 and $63.1\%$ which are 3.3 and $1.5\%$ lower than those of Hwasan 101, respectively. Acid detergent fiber and neutral detergent fiber of Hwasan 104 were 32.6 and $55.8\%$ which are 1.9 and $2.4\%$ higher than those of Hwasan 101, respectively.
This study was conducted to investigated the major characteristics of genetically modified rice of 'Milyang 204' originated from Dongjinbyeo compared to a non-transgenic rice varieties Dongjinbyeo and Jun-ambyeo. Basta resistant transgenic rice lines carrying bar gene produced by the Yeongnam Agricultural Research Institute were evaluated for their agronomic characters. The transgenic Japonica rice of 'Milyang 204' showed inferior phenotypic traits compared to a non-transgenic rice variety Dongjinbyeo and Junambyeo. On the basis of UPOV (Union Internationale Pour la Protaection des Obtentions Vegetables) and NSMO(National Seed Management Office) the transgenic 'Milyang 204' showed difference in some traits out of some agronomic traits, such as leaf color, angle of flag leaf, number of spikelets, culm length, white core and white belly compared to the non-transgenic varieties rice.
Attempts to search infection period, infection speed in the tissue of neck blast of rice plant, location of inoculum source and effects of several conditions about the leaf sheath of rice plants for neck blast incidence have been made. 1. The most infectious period for neck blast incidence was the booting stage just before heading date, and most of necks have been infected during the booting stage and on heading date. But $Indica{\times}Japonica$ hybrid varieties had shown always high possibility for infection after booting stage. 2. Incubation period for neck blast of rice plants under natural conditions had rather a long period ranging from 10 to 22 days. Under artificial inoculation condition incubation period in the young panicle was shorter than in the old panicle. Panicles that emerged from the sheath of flag leaf had long incubation period, with a low infection rate and they also shown slow infection speed in the tissue. 3. Considering the incubation period of neck blast of rice plant, we assumed that the most effective application periods of chemicals are 5-10 days for immediate effective chemicals and 10-15 days for slow effective chemicals before heading. 4. Infiltration of conidia into the leaf sheath of rice plant carried out by saturation effect with water through the suture of the upper three leaves. The number of conidia observed in the leaf sheath during the booting stage were higher than those in the leaf sheath during other stages. Ligule had protected to infiltrate of conidia into the leaf sheath. 5. When conidia were infiltrated into the leaf sheath, the highest number of attached conidia was observed on the panicle base and panicle axis with hairs and degenerated panicle, which seemed to promote the infection of neck blast. 6. The lowest spore concentration for neck blast incidence was variable with rice varietal groups. $Indica{\times}Japonica$ hybrid varieties were infected easily compared to the Japonica type varieties, especially. The number of spores for neck blast incidence in $Indica{\times}Japonica$ hybrid varieties was less than 100 and disease index was higher also in $Indica{\times}Japonica$ hybrid than in Japonica type varieties. 7. Nitrogen content and silicate content were related with blast incidence in necks of rice plants in the different growing stage changed during growing period. Nitrogen content increased from booting stage to heading date and then decreased gradually as time passes. Silicate content increased from booting stage after heading with time. Change of these content promoted to increase neck blast infection. 8. Conidia moved to rice plant by ascending and desending dispersal and then attached on the rice plant. Conidia transfered horizontally was found very negligible. So we presumed that infection rate of neck blast was very low after emergence of panicle base from the leaf sheath. Also ascending air current by temperature difference between upper and lower side of rice plant seemed to increase the liberation of spores. 9. Conidial number of the blast fungus collected just before and after heading date was closely related with neck blast incidence. Lesions on three leaves from the top were closely related with neck blast incidence, because they had high potential for conidia formation of rice blast fungus and they were direct inoculum sources for neck blast. 10. The condition inside the leaf sheath was very favorable for the incidence of neck blast and the neck blast incidence in the leaf sheath increased as the level of fertilizer applied increased. Therefore, the infection rate of neck blast on the all panicle parts such as panicle base, panicle branches, spikelets, nodes, and internodes inside the leaf sheath didn't show differences due to varietal resistance or fertilizers applied. 11. Except for others among dominant species of fungi in the leaf sheath, only Gerlachia oryzae appeared to promote incidence of neck blast. It was assumed that days for heading of varieties were related with neck blast incidence.
Although the young spike of barley (Hordeum vulgare L.) or wheat (Triticum aestivum L.) is known as the most susceptible part to spring cold injury, the risk of cold injury is apt to be ignored in most breeding program due to the importance of early maturity. Based on these aspects, the types and inducing time, temperature conditions for induction and effects of cold injury on growth and yield in this study were investigated under greenhouse and field conditions through three years (1997-1999). In natural condition, low temperature around -2.4∼$-10.2^{\circ}C$ caused the death of plant. Several cold injury types such as partial degeneration of spike, partial discoloration of leaf, spike and awn, discoloration of culm and white spike were observed at low temperature around $-3.1^{\circ}C$. Low temperature around -2.4∼$-8.6^{\circ}C$ and 1.3-$7.6^{\circ}C$ caused degeneration and sterility of spike, respectively. Most materials were prepared to the spikelet foundation stage, spikelet differentiation stage, development stage of flower organ, booting stage and heading stage, which were known having risk for cold injury in field condition. Although most of the controlled stages were sensitive to the induced low temperature, booting stage was the most sensitive stage for cold injury. All of growth stages which were treated-heading stage, booting stage, development stage of flower organ, spikelet differentiation stage, spikelet foundation stage-were responded to low temperature treatment but the symptoms revealed were very specific according to the growth stages. Ears of plant in heading stage were discolored to white. Ears of plant in booting stage were degenerated in all or part of one. Plants in spikelet differentiation stage were sterile in all or part of one. When tried to detect the specific differences between normal and cold injured plants in appearance, spike length, distance between spike and flag leaf and the first internode length could be the critical points for occurrence of spike death caused by cold injury. In barley, the elongation of spike was stopped on 3.2cm after occurrence of spike degeneration, 4.7cm after occurrence of partial degeneration of spike, 5.0cm after occurrence of white spike. In wheat, it was stopped on 1.6cm after occurrence of stem death, 3.3cm after occurrence of spike degeneration, 8.3cm after occurrence of partial degeneration of spike, 8.1cm after occurrence of white spike, 7.5cm after partial discoloration of leaf and 9.3cm after partial discoloration of spike. The obtained results from low temperature treatment induced in growth chamber were similar to the field experiment, Beacuse the death of spikes was more when low temperature was treated two times than one times, the temperature should be upgrade to -3$^{\circ}C$ in order to get the same condition with field test.
Urea and ammonium sulfate without and with sulfur group, respectively, were compared with respect to their nutritive effects on the rice plants, Suweon 264 and Jinheung, under the nursery trial on the protected upland and lowland seedbed, the pot trials with their various combination ratios and with added sodium sulfate to urea and the trials on the paddy fields which have undergone urea and ammonium sulfate application for many years. The rice seedlings fertilized with ammonium sulfate surpassed those with urea in growth performance and nutrients absorption of the rice seedlings at nursery period. Such stimulating effect of ammonium sulfate on the growth and nutrients uptake was more remarkable under the upland nursery than under the lowland one. The pot trial with the various combination ratios of urea and ammonium sulfate revealed that the chlorophyll content in leaf blade increased with the increment of sulfate ratio in the combination and the sole application of urea caused the chlorosis of leaf which was more conspicuous in Suweon 264 than in Jinheung. Fertilized with the same active ingradient amount of nitrogen, the rice plants supplied with ammonium sulfate surpassed those with urea in the chlorophyll content consistently under the nursery, the pot and the paddy field trials. The photosynthesis of flag leaf at heading stage increased with the combination rate of ammonium sulfate in the pot trial. The sulfur applicated as supplementary element of nitrogen in the nursery, the pot and the field trials were observed to be in positive relationship to the nitrogen and potassium content, but to be in negative correlation to the calcium content. The sulfur content in the rice plants was higher at early growth stage and decreased with the advance in growth stage. The nitrogen content also showed a similiar tendency to the sulfur content, and the N/S ratio was higher at early growth stage than at later one. The N/S ratio was negatively correlated with the chlorophyll content. In the field experiment, ammonium sulfate surpassed urea in the number of productive tiller, dry matter production and unhulled rice yields, but much stimulating effect of ammonium sulfate on the grain production was shown to be less effective than that on the straw production. The nitrogen and major nutrients content in the rice straw at harvest were higher in the paddy field with long-term ammonium sulfate application than in that with long-term urea application, suggesting that the former might have greater potentiality in nutrients supply than the latter.
The phenotypic traits of herbicide-tolerant transgenic rice were compared with those of wild type (Dongjin) as well as two accessions (Hwaseong-aengmi 1 and Gwangyang-aengmi 12) of weedy rice. This study was conducted to investigate whether unintentional alterations in phenotypic characteristics occurred in the transgenic rice and whether the altered traits were similar to those in the two weedy rices. All qualitative traits studied were similar in the transgenic or wild-type rice. On the other hand, awn presence, flag leaf attitude and grain color differed considerably between herbicide-tolerant transgenic rice and weedy rice. As for quantitative traits, plant height, the number of tillers per plant and shoot dry weight were significantly greater for weedy rice than transgenic or wild-type rice. Grain weight per plant and 1000-grain weight of transgenic (or wild-type) rice were significantly greater than those of weedy rice. Transgenic rice shattered less than the other rices. Amylose and protein contents in embryos of transgenic rice were significantly different from those of weedy rice. The potential for weediness of the transgenic rice may be assessed using phenotype comparison between transgenic and weedy rice as shown in this study.
The results of the investigation on the relationship between the varietal difference of photosynthesis capacity and carbohydrate metabolism and the susceptibility to sudden wilting are as follow: 1. Yushin and Milyang 23, varieties in which sudden wilting occurs easily, showed that the photosynthesis capacity at the young panicle formation stage and the contents of chlorophyll of the flag leaf were lower than Jinhung which did not show sudden wilting. 2. Respiration consumption index (${\frac{amount\;of\;respiration}{amount\;of\;assimilation}{\time}100$) of Yushin and Milyang 23 were higher than Jinhung's. 3. Regarding sugar contents of each internode at the ripening stage, the contents of the second and third internodes of Yushin were lower than Jinhung, but no varietal difference was found in the 1st internode. 4. The starch content of the Yushin node sharply decreased compared with Jinhung at the ripening stage. In Jinhung it was especially higher in lowerinternodes, but in the third internode of Yushin. was not more than 50% of that in higher internode's.
These studies were aimed at clarifying whether or not selecting agronomic characteristics of hybrid-rice populations under greenhouse conditions was useful. The selection of two quantitative characteristics, culm length and heading date, and two qualitative characteristics, shattering and awnedness, was very effective, but the flag leaf length and the exsertion of panicle was not consistent, varying from one cross to another.
This experiment was carried out to analyze the effect of different air- and soil-temperatures on the major traits of barley including grain yield. Outdoor growth cabinet and soil temperature equipment were used for this study to control the air- and soil-temperatures during the plant growth. Earlier heading appeared at the higher soil-temperature under same air-temperature, and more stems showed when conbined with 25$^{\circ}C$ of air-temperature and l0$^{\circ}C$ soil-temperature than any treatment. There appeared, however, the longest spike length, awn length, distance from flag-leaf to spike and culm length at 20$^{\circ}C$ of air-temperature and 10$^{\circ}C$ or 15$^{\circ}C$ of soil-temperatures. As far as grain yields are concerned, there showed the highest yield on the condition combined with 20$^{\circ}C$ of air-temperature and 10$^{\circ}C$ of soil-temperature.
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