Human serum albumin (HSA) is the most abundant protein in plasma and is the most often used intravenous protein in many human therapies. However, HSA is currently extracted only from plasma because commercially feasible recombinant expression systems are not available. This study attempted to develop an efficient system for recombinant HSA production by chloroplast transformation of tobacco. A HSA cDNA was isolated from a cDNA library constructed with human liver tissue. Chloroplast transformation vectors were constructed by introducing various regulatory elements to HSA regulatory sequences. Vectors were delivered by particle bombardment into leaf explants and chloroplast-transformed plants were subsequently regenerated into whole plants. Southern blot analysis confirmed that the HSA cDNA was incorporated between rps12 and orf70B of the chloroplast genome as designed. Western blot analysis revealed that hyper-expression and increasing the stability of HSA were achieved by modification of the regulatory sequences using the psbA5'UTRs in combination with elements of the 14 N-terminal amino acids of the GFP and the FLAG tag. However, only plants transformed with the vector containing all of these elements were able to accumulate HSA.
Sink (grain)-filling rates of IR667 line (Suwon 213 and 214) and local leading varieties (Jinheung and Paldal) were investigated in relation to sink-source ratio, sink-source distance, forms of photosynthates, and weather factors. The results are as follows. 1. IR667 line have higher maximum filling rate (g. grain/day. ear) and shorter duration to reach maximum filling rate than local varieties. The curve pattern of sink-filling rate was high and steep type in IR667 line and low and broad type in local varieties. 2. Weather factors seem to give little effect on the curve pattern of sink-filling rate. 3. The functional sink-source distance (actual sink-source distance multiplied by the contribution rate of leaf for grain production) was shorter in upper leaves (flag and 2nd leaf) for IR667 line, the upper leaf-dependent type in grain production, and was longer in lower leaves (3rd and 4th) for local varieties, the lower leaf-dependent type. Specially short first internode from top may contribute to the upper leaf depencency of IR667 line. 4. According to free sugar-starch ratio (sugar/starch) in the culm and leaf sheath IR667 line could be classified as the high sugar type and the local varieties as the high starch type. The ratio of transportable form (sugar) to non transportable photosynthate (starch) seems to relate with sink-filling rate. And high sugar type is expected to have higher efficiency for grain production in view of energy balance. 5. A hypothesis that the higher in the uniformity within the series of productive structure the more efficient in grain production is proposed and discussed in relation to productivity. 6. According to the pattern of percent nutrient retention of each leaf blade IR667 showed the central retention type and Jinheung showed the apical retention type and each retention type appears to be a cause of each canopy conservation pattern. 7. From the content and percent distribution of nutrient in various organ IR667 could be classified as the leaf sheath dominant type and Jinheung as the leaf blade dominant type. 8. The fact that the greater the percent translocation of nutrient into grain the greater the percent nutrient retention in leaf blade was held between nitrogen and phosphorus within a variety and between varieties within a nutrient (N, P or K).
A study was conducted in order to compare the topdressing method of the conventional fertilizers as control and the deep application method of the ball complex fertilizer newly developed. The ball complex fertilizer consisted of 5% of nitrogen, 5% of phosphorus, and 7% of potassium. Basal application of nitrogen for the rice plant was the same for both control plots and ball complex plots. One ball complex fertilizer per four hills was applied at depth of 12~13cm 35days before heading stage while control plot received three times topdressing at different growth stages as usual practice. The results obtained were as follows. 1. The ball complex fertilizer applied in the soil was continuously utilized by the rice plants until harvest time while nitrogen and potassium uptake of control plots was reduced rapidly after heading stage. Daily uptake of nitrogen and potassium per hill at maturing stage were 0.45mg and 0.68mg in control plots, but 4.80mg and 7.0mg respectively in ball complex plots. 2. Dry matter productivity of the rice plant in control plots, well coinciding with nutrients uptake pattern, was maximum just after heading stage decreased at maturing stage. But dry matter productivity in ball complex plots was much higher at maturing stage than at heading stage. 3. Ball complex application increased effective tillering rate, causing higher panicle number per hill. 4. Ball complex application brought about 528kg/10a of hulled grain yield while the conventional practice 423kg/10a. 5. Deep application of ball complex was superior to usual practice in terms of yield components such as panicle number per hill, filled grain number per panicle, maturing rate, and 1,000 grain weight. 6. From the morphological characteristics point of view, the deep application of ball complex made the flag leaf and the 2nd leaf heavier, larger and broader as compared to control treatment. 7. It is considered that by applying the ball complex fertilizer at depth of 12~13cm sufficient amount of nitrogen and potassium could be utilized by rice plants during the maturing stage and assimilated in the leaf blade, consequently making the flag leaf and the 2nd leaf bigger and healthier. The fact can easily explain that the ball complex plots had higher capacity of photosynthesis, less discoloration of lower leaves, bigger leaf area index, and better grain yield as compared to the conventional practice. In conclusion the deep application method of the ball complex fertilizer was superior to the routine topdressing method of the usual fertilizers.
This study was carried out to investigate heterosis in photosynthetic characteristics and yield of F$_1$ hybrid rice using genetic cytoplasmic male sterile lines. Two F$_1$ hybrid rices, HR7028A/Yongmoonbyeo and IR54756A/Yongmoonbyeo, showed heterobeltiosis in dry weight and leaf area at the heading date and at the 20 day after heading. HR7028A/Yongmoonbyeo showed heterobeltiosis and IR54756A/Yongmoonbyeo showed heterosis in chlorophyll content and RuDPCase activity in flag leaf, T-sugar and starch content in stem, and $CO_2$ fixation at the 20 day after heading as well as at the heading date. HR7028A/Yongmoonbyeo showed heterobeltiosis in number of panicles and grains per panicle, and IR54756A/Yongmoonbyeo had heterobeltiosis in number of panicles per plant. As results, heterobeltiosis of HR7028A/Yongmoonbyeo in yield was 11% and that of IR54756A/Yongmoonbyeo was 4%.
Proceedings of the Korean Society of Crop Science Conference
/
2017.06a
/
pp.227-227
/
2017
This study was conducted to analyze the reduction of quality and ripening rate of rice due to submergence during ripening stage and identify the physiological cause. Korean japonica type rice cultivars, Nampyeong was used in the experiment. The following 7, 14 day after heading, they were then moved into submergence treatment facility and we conducted the tests under three different submergence conditions - T1(clear water and overhead submergence), T2(Muddy water and exposure of Flag leaf end ), T3(Muddy water and overhead submergence), and the treatment lasted for 4days. The decrease of ripening rate was most severe in T3 treatment at 7days after heading, and the decrease of head rice ratio was most severe in T3 treatment at 14days after heading. Meanwhile the starch synthesis was inhibited, as the supply of assimilation products was inhibited in grain during the submergence treatment. And in stem, sucrose content was increased because the soluble carbohydrates accumulated before heading were decomposed. These changes may be due to the consumption of respiratory substrate in anaerobic conditions and the inhibition of the production of photosynthetic products by light interception. In order to see what kind of reaction occurs at the molecular level, we examined the degree of RNA expression in grain, stem and leaf. First, the expression of rna associated with starch synthesis in grain was decreased in all submergence treatment. and especially Ospul(DBE) was more decreased in 14days after heading treatment than 7days after heading treatment. This difference can explain the result that the decrease of head rice ratio was more severe at 14days after heading. And in stem, the expression of rna associated with the supply of assimilation products was decreased in submergence treatment. Finally in leaf, the expression of rna(ADH, ALDH) associated with anaerobic respiration was increased, while the expression of rna associated with photosynthesis was decreased. These results of physiological analysis can used to develop the cultivation technique and to offer the information for breeding the cultivars with tolerant characteristics to submergence stress during ripening stage in rice.
Ten recommended rice varieties were cultivated in paddy area affected by air-pollutants and in normal area to select varieties adaptable to air-pollution environment. Rice plants grown in pollution site showed higher contents of total sulfur and fluorine in leaf through the whole growing period compared with those in non-pollution site, and rice leaves destroyed by air-pollutants were found only in pollution site. Rice grain yield and four yield components of ten rice varieties grown in pollution site were lower than those in normal area. Five rice varieties among ten were selected as adaptable to air-pollution environment, based on their yielding potential in pollution site and grain yield ratio between two sites. Rice varieties adaptable to pollution showed little variation of percent ripened grains and number of panicles per hill between two sites. Chlorophyll content in flag leaf of rice plants grown in pollution area was lower than in non-pollution area. No relationship was found between grain yield ratio (pollution/non-pollution site) of ten varieties and total sulfur content ratio, fluorine content ratio, chlorophyll content ratio between two sites, and percent destroyed leaf in pollution site, respectively. This result suggest that varietal adaptability to air-pollution environment is not related with the amount of pollutants absorbed, but with the degree of response to pollutants.
The effect of various supplementary light sources on the agronomic characteristics has been investigeted for generation acceleration of barley in green house with the condition of 15$^{\circ}C$ and 8 hrs. day light followed by each supplementary light sources including incandescent electric lamp (IN), Blue fluorescent lamp(BF). day-light fluorescent lamp(DF), Biolux lamp(BL), BL+BF, In+BF and IN+BL. Leaf number of main stem, leaf area, culm length, dry matter yield of shoot, number of grain per spike, grain yield per plant were most sharply decreased with the exposition of IN, while flag-leaf emergence on main stem and I, 000 grain weight were earlist and heavist respectively with the exposition of IN. In appeared to be best light source for shortening the days to heading, but IN + BF will be most desirable light source with considering both days to heading and adequate yield at the same time.
Ten isolates of Xanthomonas oryzae were inoculated to three rice varieties; 'Milyang 23' in Kinmaze group, 'Yushin' in Kogyoku group and 'Tongil' in Rantai-emas group, at the seedling stage, early-tillering stage, Maximum-tillering stage and flag-leaf stage. Much fluctuation was existed in virulence pattern of isolates at each growth stage. Especially, the isolates of pathotype II showed much more variation in virulence. This suggests that there would be more sub-divided pathotypes involved in pathotype II. Isolate G 7716 of pathotype II showed its virulence to 'Yushin' variety only after booting stage. On the result of the analysis of variance for the reaction of three rice varieties to three isolates at each growth stage; the isolates, varieties, growth stages were the main factors of variations of virulence, and the interaction of isolates with varieties was significant but other interactions were not.
The objective of this study was to ,determine trinexapac-ethyl [4-(cyclopropyl- $\alpha$ -hydroxy-me-thylene)-3,5-dioxocyclohexanecarboxylic acid ethyl ester] effects on lodging-related traits of rice varieties treated at different application rates and times. Trinexapac-ethyl WP was applied at the rate of 10, 20 and 30g ai/l0a to Heukjinjubyeo and Daeanbyeo, respectively. Effects of trinexapacethyl varied greatly with application time as well as application rate with little differences between rice varieties. As the application rate increased, lodging index, center of gravity and exsertion of panicle above flag leaf decreased, whereas breaking strength and phytotoxicity increased. Exsertion of panicle was greatly influenced by the application time with a little difference by application rate. The most effective application time of trinexapac-ethyl based on lodging index and total internode length (Ist-4th node) was 2DBH(days before heading) in Heukjinjubyeo and 17DBH in Daeanbyeo, resulting in reducing total internode length by 12.9 and 9.7cm, respectively. A significant reduction was found at the 1st, 2nd, and 3rd internodes in Heukjinjubyeo, while at the 2nd and 3rd internodes in Daeanbyeo. No significant difference was found among treatments in the panicle length of both rice varieties but the panicle fresh weights of Daeanbyeo were significantly greater in all trinexapac-ethyl treatments compared with that of the control when measured 20 days after heading. Consequently, trinexapacethyl treatment significantly improved lodging-related traits of rice plants without affecting rice yield and yield components.
Behaviour of a fungicide $^{14}C-propiconazole$ was investigated in a field lysimeter of sandy loam soil. At 15 days after rice-seedling transplanting, $^{14}C-propiconazole$ was treated on the soil surface at the rate of 0.12 kg/10a. The cummulative $^{14}C-radioactivity$ in the leachate from the lysimeter soil was 4.4% of the applied $^{14}C$ for 16 weeks. Most radioactivity detected in leachate was in the form of parent compound. At the end of lysimeter experiment. $^{14}C$ radioactivity in lysimeter soil was 76.5% of the applied $^{14}C$ and more than 97% of $^{14}C$ in soil remained in the top 20 cm. The percent of $^{14}CO_2$ evolved from lysimeter soil was 7.8% of the applied $^{14}C$. The radioactivity remained in the rice straw after harvest was 7.2% of the applied $^{14}C$, while less than 0.1% of the applied $^{14}C$ was detected in flag leaf, ear, chaff and hulled rice, respectively.
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