• Journal of Microbiology
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• v.35 no.3
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• pp.181-187
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• 1997

Filamentous cyanobacteria are an ecologically important group of bacteria because they are able to provide both organic carbon fixed nitrogen that can support the nutritional requirements for other microorganisms. Because of their prokaryotic nature, they can also be used as potentially powerful model systems for the analysis of oxygenic photosynthesis and nitrogen fixation. Gene transfer is an indispensable procedure for genetic analysis of filamentous cyanobacteria. Electroporation was used to introduce foreign DNA into cyanobacterial cells. In experiments designed to optimize the electroporation technique, the effects of the field strength (amplitude of pulse) and time constant (duration of pulse), DNA concentration and host restriction/modification of DNA on the efficiency of electro-transformation were investigated. The results of this research revelaed that a high voltage pulse of short duration was effective for the electro-transformation of Anabaene sp. M131. The maximal number of transformants was obtained at 6 kV/cm with a pulse duration of 5 msec. The efficiency of electro-transformation was also sensitive to concenetration of DNA; even small amounts of DNA (0.01 .mu.g/ml) were able to gie a large number of transformants (1.0 * 10$\^$3/ cfu/ml).

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 1996.10a
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• pp.217-223
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• 1996

A structure's vibration characteristic is determined by modal property of the system. Through proper vibration analysis or experiments, the structure can be modified to reduce of vibration and noise. This paper is concerned with the natural frequency and modal loss factor of sandwich plates with viscoelastic core. The effects of shear and normal strain in the viscoelastic layer are investigated on modal properties, natural frequency and modal loss factor, by changing geometry parameter and viscoelastic material property of sandwich plates. The errors of modal parameters resulting from neglecting the extension or compression in the core material for simply supported(S-S-S-S) case are compared with those for clamped(C-C-C-C) boundary condition. Finite difference method(FDM) is utilized as numerical analysis technique of square sandwich plates for fixed boundary conditions. In order to reduce computation time and increase accuracy, improved finite difference expression with fourth order truncation error was used.

• Proceedings of the KAIS Fall Conference
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• 2008.05a
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• pp.157-159
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• 2008

This paper has been studied traffic accident using intelligence prediction algorithm. and wish to prevent accident by guiding in 2 km ahead the accident that occur in fog section and a snow-covered road, sudden roadworks and sharp curve section, etc and removing fog and snow automatically using the ubiquitous and intelligence technique. If we can predict of traffic accident, we can prevent the many traffic accident. In this paper, we present neural network approach for prediction of traffic accident. Computer simulation results prove that reducing the average vehicle waiting time which proposed considering prevention of traffic algorithm for optimal traffic cycle is better than fixed signal method which dose not using prevention of traffic algorithm.

• Journal of Power Electronics
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• v.18 no.6
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• pp.1720-1728
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• 2018

This paper proposes a novel soft starting algorithm by using PWM inverter technique to control an amplitude of the motor starting current at a single-phase induction motor (SPIM). Traditional SPIM starting methods such as a Split-Phase, Capacitor-Start, Permanent-Split Capacitor (PSC), Capacitor-Start Capacitor-Run (CSCR), basically cannot control the magnitude of starting current due to the fixed system structures. Therefore, in this paper, a soft starting algorithm based on a proportional resonant (PR) control with a variable and constant frequency is proposed to reduce the inrush current and starting up time. In addition, a transition algorithm for operation modes is devised to generate a constant voltage and constant frequency (CVCF). The validity and effectiveness of the proposed soft starting method and transition algorithm are verified through experimental results.

• Journal of Advanced Navigation Technology
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• v.26 no.5
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• pp.304-311
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• 2022

Although the SEAD(suppression to enemy air defenses) mission is a strategically important task in modern warfare, the high risk of direct exposure to enemy air defense assets forces to use of unmanned aerial vehicles. this paper proposes a path planning algorithm for SEAD mission for an unmanned aerial vehicle and a method for calculating the mission effectiveness on the planned path. Based on the RRT-based path planning algorithm, a low-altitude ingress/egress flight path that can consider the enemy's short-range air defense threat was generated. The Dubins path-based Intercept path planning technique was used to generate a path that is the shortest path while avoiding the enemy's short-range anti-aircraft threat as much as possible. The ingress/intercept/egress paths were connected in order. In addition, mission effectiveness consisting of fuel consumption, the survival probability, the time required to perform the mission, and the target destruction probability was calculated based on the generated path. The proposed techniques were verified through a scenario.

• The Journal of Korean Academy of Prosthodontics
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• v.56 no.2
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• pp.173-178
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• 2018

The direct metal laser sintering (DMLS) technique would be promising for the full-arch implant-supported restorations due to reduced cost and manufacturing time without potential human errors and casting defects. The aims of this case report were to describe the successful outcome of an implant-supported fixed dental prosthesis in the edentulous maxilla by using the DMLS technology and computer-aided design and computer-aided manufacturing (CAD/CAM) monolithic zirconia crowns, and to describe its clinical implications. A healthy 51-year-old Korean woman visited Seoul National University Dental Hospital and she was in need of a rehabilitation of her entire maxilla due to severe tooth mobility. In this case, all maxillary teeth were extracted and an implant-supported fixed dental prosthesis was fabricated that involved a cobalt-chromium (Co-Cr) framework with the DMLS technique and CAD/CAM monolithic zirconia crowns. Six months after delivery, no distinct mechanical and biological complications were detected and the prosthesis exhibited satisfactory esthetics and function. In this case report, with the DMLS system, the three-dimensional printed prosthesis was created without additional manual tooling and thus, reliable accuracy and passive fit were obtained.

• Journal of Information Technology Applications and Management
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• v.11 no.2
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• pp.29-43
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• 2004

As the development of an information technique, gradually, mobile device is going to be miniaturized and operates at high speed. By such the requirements, the devices using a flash memory as a storage media are increasing. The flash memory consumes low power, is a small size, and has a fast access time like the main memory. But the flash memory must erase for recording and the erase cycle is limited. JFFS is a representative filesystem which reflects the characteristics of the flash memory. JFFS to be consisted of LSF structure, writes new data to the flash memory in sequential, which is not related to a file size. Mounting a filesystem or an error recovery is achieved through the sequential approach. Therefore, the mounting delay time is happened according to the file system size. This paper proposes a MJFFS to use a multi-checkpoint information to manage a mass flash file system efficiently. A MJFFS, which improves JFFS, divides a flash memory into the block for suitable to the block device, and stores file information of a checkpoint structure at fixed interval. Therefore mounting and error recovery processing reduce efficiently a number of filesystem access by collecting a smaller checkpoint information than capacity of actual files. A MJFFS will be suitable to a mobile device owing to accomplish fast mounting and error recovery using advantage of log foundation filesystem and overcoming defect of JFFS.

• The Transactions of the Korean Institute of Electrical Engineers B
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• v.49 no.6
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• pp.387-394
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• 2000

This paper presents a method of cold gas flow-field analysis within puffer type GCB(Gas Circuit Breaker). Using this method, the entire interruption process including opening operation of GCB can be simulated successfully. In particular, the distortion problem of the grid due to the movement of moving parts can be dealt with by the fixed grid technique. The gas parameters such as temperature, pressure, density, velocity through the entire interruption process can be calculated and visualized. It was confirmed that the time variation of pressure which was calculated from the application of the method to a model GCB agreed with the experimental one. Therefore it is possible to evaluate the small current interruption capability analytically and to design the interrupter which has excellent interruption capability using the proposed method. It is expected that the proposed method can reduce the time and cost for development of GCB very much. It also will be possible to develop the hot-gas flow-field analysis program by combining the cold-gas flow field program with the arc model and to evaluate the large current interruption capability.

• Transactions on Electrical and Electronic Materials
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• v.6 no.4
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• pp.135-139
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• 2005

In this paper, negative SU-8 photoresist processed at low temperature is characterized in terms of delamination. Based on a $3^3$ factorial designed experiment, 27 samples are fabricated, and the degree of delamination is measured for each. In addition, nine samples are fabricated for the purpose of verification. Employing the. neural network modeling technique, a process model is established, and response surfaces are generated to investigate degree of delamination associated with three process parameters: post exposure bake (PEB) temperature, PEB time, and exposure energy. From the response surfaces generated, two significant parameters associated with delamination are identified, and their effects on delamination are analyzed. Higher PEB temperature at a fixed PEB time results in a greater degree of delamination. In addition, a higher dose of exposure energy lowers the temperature at which the delamination begins and also results in a larger degree of delamination. These results identify acceptable ranges of the three process variables to avoid delamination of SU-8 film, which in turn might lead to potential defects in MEMS device fabrication.

• Proceedings of the KSME Conference
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• 2003.04a
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• pp.764-769
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• 2003

The paper introduces a development result for displacement measurement system of multiple moving objects based on image processing technique. The image processing method adopts inertia moment theory for obtaining the centroid of the targets and basic processing algorithms of gray, binary, closing, labeling and etc. To get precise displacement measurement in spite of multiple moving targets, a CCD camera with zoom is used and the position of camera is changed by a pan/tilt system. The fiducial marks on the fixed positions are used as the sensing points for the image processing to recognize the position errors in directions of X -Y coordinates. The precise alignment device is pan /tilt of X - Y type and the pan/tilt is controlled by DC servomotors which are driven by 80c196kc microprocessor based controller. The centers of the fiducial marks are obtained by a inertia moment method. By applying the developed precise position control system for multiple targets, the displacement of multiple moving targets are detected automatically and are stored in the database system in a real time. By using database system and internet, displacement data can be confirmed at a great distance and analyzed. The developed system shows the effectiveness such that it realizes the precision about 0.12mm in the position control of X -Y coordinates.