• 한국동물학회:학술대회논문집
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• 한국동물학회 1997년도 한국생물과학협회 학술발표대회
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• pp.276.2-276
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• 1997

No Abstract, See Full Text

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 2005년도 추계학술발표회
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• pp.29-30
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• 2005

• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
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• pp.251.2-251
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• 1999

No Abstract, See Full Text

• 한국방사성폐기물학회:학술대회논문집
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• 한국방사성폐기물학회 2017년도 춘계학술논문요약집
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• pp.271-272
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• 2017

• 한국방사성폐기물학회:학술대회논문집
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• 한국방사성폐기물학회 2017년도 춘계학술논문요약집
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• pp.223-224
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• 2017

• Molecules and Cells
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• 제20권1호
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• pp.74-82
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• 2005

Glutaredoxins (Grxs) are thioloxidoreductases which are required for maintaining thiol/disulfide equilibrium in living cells. The Grx3 gene, which encodes one of the three monothiol Grxs in the fission yeast Schizosaccharomyces pombe, was characterized, and its transcriptional regulation studied. Genomic DNA encoding Grx3 was isolated by PCR, and a plasmid pTT3 carrying this DNA was produced. The DNA sequence has 1,267 bp, which would encode a monothiol Grx of 166 amino acids with a molecular mass of 18.3 kDa. The putative protein has 27% homology with Grx5, and contains many hydrophobic amino acid residues in its N-terminal region. S. pombe cells harboring pTT3 had increased Grx activity and enhanced survival on minimal medium plates containing aluminum (5 mM), BSO (0.05 mM), menadione (0.01 mM) or cadmium (0.2 mM). The 568 bp upstream region of Grx3 was fused into the promoterless b-galactosidase gene of the shuttle vector YEp367R to generate fusion plasmid pMJS10. Potassium chloride (KCl) and metals including aluminum and cadmium enhanced the synthesis of ${\beta}$-galactosidase from the fusion gene. The synthesis of ${\beta}$-galactosidase was also enhanced, in a Pap1-dependent manner, by fermentable carbon sources such as glucose (at low concentrations) and sucrose, but not by non-fermentable carbon sources such as ethanol and acetate. Grx3 mRNA increased in response to treatment with BSO. These observations indicate that S. pombe Grx3 is involved in the response to stress, and is regulated by stress.

• Journal of Radiation Protection and Research
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• 제1권1호
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• pp.1-9
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• 1976

핵임계사고시(核臨界事故時)에 방출(放出)되는 속중성자(速中性子)가 산란중성자(散亂中性子)로 중첩(重疊)되어 있는 상태(狀態)에서 방사화(放射化) 및 발단방사화검출기(發端放射化檢出器)를 이용(利用)하여 속중성자(速中性子)를 측정(測定) 및 해석(解析)할 수 있는 한 방법(方法)을 제안(提案)하였으며 이 측정(測定)에 있어서 주요인자(主要因子), 즉(卽) 몇개의 발단방사화검출기(發端放射化檢出器)에 대(對)한 평균핵반응단면적(平均核反應斷面積)과 중성자당선량환산계수(中性子當線量換算係數)를 전자계산기(電子計算機)로 계산(計算)하였다. 그 결과(結果) 핵분열중성자(核分裂中性子)의 스펙트럼 측정(測定)에는 발단(發端)에너지가 높은 검출기(檢出器)가 유리(有利)한 것에 반(反)해 발단(發端)에너지가 낮은 것은 산란매질(散亂媒質)이 없는 핵임계장치(核臨界裝置)의 사고시(事故時)에 있어서 속중성자(速中性子)의 시적분선속밀도(時積分線束密度) 측정계(測定計) 유용(有用)한것 같았다. 그리고 유황(硫黃)의 (n, p) 핵분열(核分裂)에 대(對)한 평균단면적(平均斷面積)은 핵분열(核分裂) 중성자(中性子)의 해석적(解析的) 표현식(表現式)에 무관(無關)한 것 처럼 보였다 .그밖에 중성자당(中性子當) 선량환산계수(線量換算係數)의 변화(變化)는 핵분열(核分裂) 중성자(中性子) 스펙트럼의 해석적(解析的) 표현식(表現式)과 핵분열상태(核分裂形態)에 따라 민감(敏感)하게 변화(變化)되지 않은 것 같았다.

• Journal of Microbiology
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• 제44권1호
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• pp.35-41
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• 2006

The unique gene encoding thioredoxin reductase (TrxR) was previously cloned and characterized from the fission yeast Schizosaccharomyces pombe, and its expression was induced by oxidative stress. To elucidate tbe regulatory mechanism of the S. pombe TrxR gene, three fusion plasmids were generated using polymerase chain reaction: pYUTR20, pYUTR30, and pYUTR40. Plasmid pYUTR20 has an upstream region of 891 base pairs, pYUTR30 has 499 in this region, and pYUTR40 has an 186 bp upstream region. Negatively acting sequence is located between $-1,526\;\~\;-891bp$ upstream of the gene. The upstream sequence, responsible for the induction of TrxR by menadione (MD), is situated on the $-499\;\~\;-186bp$ region, which is also required for TrxR induction by mercuric chloride. The same region also appeared to be required for Pap1-mediated transcriptional regulation of the TrxR gene, which contains the two plausible Papl binding sites, TTACGAAT and TTACGCGA. Consistently, basal and inducible expression of the TrxR gene was markedly lower in the Pap1-negative TP108-3C cells than in wild-type yeast cells. In summary, up-regulation of the S. pombe TrxR gene is mediated by Pap1 via the transcriptional motif(s) located on the $-499\;\~\;-186bp$ region.

• BMB Reports
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• 제36권3호
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• pp.326-331
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• 2003

The fission yeast cells that contained the cloned glutathione synthetase (GS) gene showed 1.4-fold higher glutathione (GSB) content and 1.9-fold higher GS activity than the cells without the cloned GS gene. Interestingly, $\gamma$-glutamylcysteine synthetase activity increased 2.1-fold in the S. pombe cells that contained the cloned GS gene. The S. pombe cells that harbored the multi copy-number plasmid pRGS49 (containing the cloned GS gene) showed a higher level of survival on solid media with cadmium chloride (1 mM) or mercuric chloride ($10\;{\mu}M$) than the cells that harbored the YEp357R vector. The 506 bp upstream sequence from the translational initiation point and N-terminal8 amino acid-coding region were fused into the promoteriess $\beta$-galactosidase gene of the shuttle vector YEp367R to generate the fusion plasmid pUGS39. Synthesis of $\beta$-galactosidase from the fusion plasmid pUGS39 was significantly enhanced by cadmium chloride and NO-generating S-nitroso-N-acetylpenicillamine (SNAP) and sodium nitroprusside (SN). It was also induced by L-buthionine-(S,R)-sulfoximine, a specific inhibitor of $\gamma$-glutamylcysteine synthetase (GCS). We also found that the expression of the S. pombe GS gene is regulated by the Atf1-Spc1-Wis1 signal pathway.

• 방사성폐기물학회지
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• 제11권1호
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• pp.31-39
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• 2013

핵연료는 원자로 운전 중 예기치 못한 상황에서 연료 결함을 초래할 수 있다. 핵연료 결함은 연료봉의 수소화나 이물질에 의한 금속 마모, 그리고 펠렛과 피복관의 상호작용에 의해 피복관이 손상된다. 이렇게 손상된 핵연료의 결함원인을 규명하는 것은 원자력발전의 안전운전에 중요하다고 사료된다. 핵연료가 손상되면 원자로 냉각재가 오염되어 원자로 출력을 낮추거나, 발전소를 정지할 수도 있다. 모든 사용후연료는 건식저장고로 이동 보관되어야 하나, 결함연료는 이동할 수 없으므로 이 연구의 목적은 중수로형 원자로에서 연료가 인출된 후 사용후연료 저장조에서 보관된 연료에 대하여 결함 여부를 판단할 수 있는 기술을 개발하고자 하였다. 이 연구를 통하여 핵종 누설 검출 기술을 이용한 사용후연료 검사기술을 개발하였으며, 이 기술을 월성발전소에 적용함으로써, 검사기술 및 검사시스템에 대한 성능을 입증하였다.