• 생명과학회지
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• 제21권12호
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• pp.1716-1720
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• 2011

섬유소를 비롯한 단백질, 지질, 녹말을 분해할 수 있는 세균을 된장으로부터 분리하여 동정한 결과 Bacillus subtilis로 분류되었으며, Bacillus subtilis CK-2로 명명하였다. 분리균주는 $40\sim45^{\circ}C$의 비교적 넓은 온도 범위와 pH 6~9의 넓은 pH 범위, 그리고 NaCl 0~3% 범위에서 잘 자랐으며, 높은 자가분해효소 활성을 갖는 것을 알 수 있었다. B. subtilis CK-2가 분비하는 가수분해효소들은 대부분 세균의 생장과 거의 비례적으로 세포외 활성을 나타내는 1차 대사산물로 확인되었다. 이상의 결과로부터 B. subtilis CK-2는 농수임산물 폐기물이나 음식물 폐기물의 퇴비화, 사료 생산 등에 유용하게 이용될 수 있을 것으로 생각한다.

• Journal of the Korean Data and Information Science Society
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• 제20권3호
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• pp.551-562
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• 2009

수협의 어선조업정보는 국가 공식 통계가 가지고 있지 못한 위치별 조업정보를 가지고 있다는 장점이 있다. 위치별 조업정보는 해당 지역의 어업피해보상, 자원가치 산출 등을 추정할 수 있어 국가통계자료로의 가치가 매우 높으나 어업인들의 자기 정보의 노출에 대한 기피로 인해 신뢰성이 떨어지는 단점을 지니고 있다. 본 연구는 유용성분석을 통해 이러한 수협의 어선조업정보의 활용을 위한 방안을 제시하고 위치별 조업패턴의 특성을 분류하여 해구별 유사성의 정보를 산출을 목적으로 하였다. 분석결과 수협의 어선조업정보는 정부 생산통계대비 어획량의 약 33% 수준이나 유용성 분석에서 그 패턴과 상관관계가 밀접해 위치별 패턴파악에 유용한 것으로 나타났다. 이를 바탕으로 대해구별 2단계 군집분석을 통해 어획량, 조업일수, 조업척수에 대해 각각 최적의 군집을 구분하고 이를 종합하여 8개의 군집으로 패턴을 구분하였다.

• 한국식품과학회지
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• 제30권6호
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• pp.1409-1414
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• 1998

Zearalenone에 특이한 단크론성 항체를 개발하기위하여 형질세포종세포인 myeloma cell $(P3{\times}63Ag.V653)$과 zearalenone-oxime-bovine serum albumin (BSA) 결합체를 항원으로 면역시킨 BALB/c female mice의 비장세포를 융합시켜 hybridoma cell을 생산하였다. 그들의 항체가를 indirect competitive ELISA법으로 측정한 결과 zearalenone에 대하여 높은 친화력을 갖는 5세포주를 얻었다. 그중 Z-2-M26 hybridoma cell line이 생산하는 단크론성항체는 특히 zearalenone과 민감하게 반응하였고, ${\alpha}-zearalenol$과도 약간의 교차반응을 보였으나 ${\beta}-zearalenol,\;{\alpha}-zearalenol,\;{\beta}-zearalenol$ 및 DON과는 거의 반응하지 않았다. 따라서 본 실험에서 개발된 항체는 앞으로 zearalenone에 대한 민감하고 정량적인 효소면역측정법의 개발을 위한 중요한 면역시약으로 사용될 것으로 생각한다.

• 한국농공학회지
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• 제13권4호
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• pp.2471-2478
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• 1971

Go Cong Water Control Project was conducted on its preliminary survey and design by Agricultural development Corporation for the Korean Government, an Executing Agency, and Directorate of Irrigation and Rural Engineering of the Ministry of Land Reform, Agriculture and Fishery Development for the Vietnamese Government, a cooperation Agency, under Korean and Vietnamese Economic and Technical Cooperation Program. The main purposes of the project are aimed at the improvements of irrigation and drainage, and salt water prevention of the Go Cong area located at northern part of the Mekong Delta. All the works from field survey through design to preparing reports were carried out by ADC alone and recently Korean Government submitted the relevant reports to vietnamese Government through official channel. The contents of the project are summarized as following: 1. The project comprises irrigation, drainage and salt water prevention facilities on the benefited area of about 55,000 hectares, covering Go Cong and Dinh Tuong(My Tho) Provinces and it will be possible to cultivate rice cropping twice a year, irrigating all the area in the dry season; 2. With completion of this project, annual production of rough rice and vegetables are anticipated to increase by 222,600 .T. and 142,600 M.T. respectively and the internal rateof return stants at 26 per cent, applying for the exchange rate of US $ 1 to VN $ 275. 3. Total investments required for the project are estimated at US $ 56,394,000 of which actual construction cost is estimated at US $ 39,183,000. The project has planned to be d to be developed by four stages, taking bout 7 years. 4. The project needs for three places of pumping plants. 57Km of feed and main irrigation canals, 81Km of drainage channels, 97Km of dike, 23 places of sluices and navigation locks, etc.

• Journal of Microbiology and Biotechnology
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• 제29권8호
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• pp.1324-1334
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• 2019

Fish mycobacteriosis is a common bacterial disease in many species of freshwater and marine fish and has caused severe loss of fish production. Mycobacterium marinum has been the most prevalent pathogen observed in several outbreaks of mycobacteriosis of farmed sturgeons in China. However, the immune responses and pathology of sturgeons in mycobacterial infection are rarely studied. Therefore, we used the Illumina RNA-seq method to analyze the transcriptome profile of Acipenser schrenckii challenged with Mycobacterium marinum. To begin, 168,220 non-redundant contigs were acquired from the infection and control groups, and among these, 33,225 contigs have acquired annotations. A total of 4,043 differently expressed (DE) contigs between the two groups were identified, and among these, 2479 were up-regulated and 1564 were down-regulated in the infected fish. A total of 1,340 DE contigs with acquired annotations in KEGG were enriched for 124 pathways including the TNF signaling pathway, and the Toll-like receptor signaling pathway. The roles of DE genes involved in significant pathways and other processes were discussed. The 2,209 DE contigs that have yet to acquire proper annotation may represent candidate genes associated with infection in sturgeons and are expected to serve as immunogenetic resources for further study. To our best knowledge, this is the first transcriptome study on sturgeons under bacterial infection.

• 한국어병학회지
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• 제34권1호
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• pp.55-61
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• 2021

One of the major problems in fish production is that parasite-induced episodes of disease can result in significant impacts to the health of stock, mortalities and, economical losses. Seabreams are economically important fish in both the capture fishery and aquaculture sectors in Oman and represent potential species for aquaculture. The current study set out to investigate infection of polyopisthocotylean monogeneans on the gills of wild goldlined seabream, Rhabdosargus sarba, caught from Musairah region in Oman. The prevalence, mean intensity and abundance of polyopisthocotyleans on 145 fish was found to be 52.41%, 5.65 and 3.04, respectively. Three species of microcotylid parasite were isolated, one being Atrispinum acarne while the remaining two species require further study to identify them. There was no significant difference in the infection rate between host sex, however, parasite burdens were higher on larger sized fish (p<0.05) and were found to favour the first gill arch (p< 0.05). The study found that the sample of Rhabdosargus sarba landed in the waters around Musairah are heavily infected, with a predominantly immature population of microcotylids. Further work is required to better understand the potential risk each species poses to the health of its host before a decision is made on the suitability of new candidate fish species for commercial aquaculture.

• 한국수산과학회지
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• 제54권2호
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• pp.194-207
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• 2021

It is necessary to assess and manage the different elements of the marine ecosystem, such as climate change, habitat, primary and secondary production, energy flow, food web, potential yield, and fishing, to maintain the health of the ecosystem as well as support sustainable development of fishery. We set up an ecosystem model around the Korean peninsula to produce scientific predictions necessary for the assessment and management of marine ecosystems and presented the usability of the model with scenario experiments. We used the Atlantis ecosystem model based on the marine food web; Atlantis is a three-dimensional end-to-end model that includes the information and processes within an entire system, from an abiotic environment to human activity. We input the ecological and biological parameters, such as growth, mortality, spawning, recruitment, and migration, to the Atlantis model via functional groups using existing research and local measurements. During the simulation period (2018-2019), we confirmed that the model reproduced the observed data reasonably and reflected the actual ecosystem characteristics appropriately. We thus identified the usability of a marine ecosystem model with experiments on different environmental change scenarios.

• 한국수산과학회지
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• 제54권6호
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• pp.1030-1035
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• 2021

Diving fishermen are demanding permission to use an inspirator that can efficiently catch Manila clam; however, coastal fishermenargue that the use of inspirator devastates the seabed and depletes fishery resources. Therefore, the aim of this study was to evaluate the fishing power of diving gears and its effect on coastal fishing grounds. The average time taken to fish in the seabed (4 m²) with a rake, water gun, and an inspirator was 11.17 min, 13.33 min and 7.81 min, respectivelyThe values of catch per unit time with a rake, water gun, and an inspirator were 3.35 kg/min, 2.26 kg/min, and 3.83 kg/min, respectively. Suspended sediments were observed the most in the water gun fishing area, and were observed more in April when fishing was not carried out than in February when fishing was carried out. However, the difference in the amount of sediment deposited in the fishing area using the three-fishing gears was very small. For an accurate analysis of the impact of the fishing gears on the seabed, supplementary tests such as grain size analysis and changes in benthic organisms need to be conducted.

• 한국수산과학회지
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• 제55권6호
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• pp.946-953
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• 2022

Using the management strategy evaluation methods and data on the Korea chub mackerel Scomber japonicus where the state-space logistic production model is used as the operation and the estimation model respectivley, we examined the effects of both Dorn's rule, α and the buffer value for ABC (allowable biological catch), which are used by the Korea fishery managers for decision rules. We set scenarios that have different pairs of buffer and α values, which include those currently used in the management in Korea. Under each sceanario, we projected the fish population biomass until year 2050, during which ABC is determined in each year with the decision rule. We used three kinds of performance measures: (i) whether the biomass in 2050 is overfished; (ii) the average of annual yields over the simulation period; and (iii) the variability of annual yields over the period. We found that the current practice (buffer=0.9, and α = 0.05) resulted in the best performance in terms of avoiding the "overfished" status. However, the current practice failed to reach the maximum average of the annual yields and led to larger uncertainty in the annual yields.

• 대한토목학회논문집
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• 제43권5호
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• pp.675-687
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• 2023

한반도 비무장지대에 인접한 접경지역은 DMZ(DemilitarizedZone) 그린평화 지대화를 위한 그린데탕트(GreenDétent) 실천무대로 주목 받고있다. 본논문은 접경지역에 계획된 생태·환경 분야 산업·인프라 사업의 복합적 성격을 판단 할 수있는 항목으로 구성된 프로슈밍지표(Prosuming-index)를 제안하고, 이 를 통해 그린데탕트 실천 대상지로서의 잠재력과 방향성을 시각화 하였다. 그 결과, 접경 지역중 인천·강화 지역은 '물류·인적 네트워크 중심 권역이자 특화 농수산물 생산지' 로서, 경기 북부지역은 '스마트기술 활용 산업을 기반으로하는 교류 네트워크 중심권역' 으로서, 강원 서부지역과 경기 연천 지역은 '탄소 감축 기술을 골자로 하는 생산-소비 복합권역' 으로서, 강원 동부지역은 '재생에너지 산업을 통한 유휴·노후 인프라의 그린 인프라 전환권역' 으로서, 크게 4가지 권역으로 그린데탕트 실천 잠재력을 유형화 할 수 있었다.