• Title/Summary/Keyword: Fisheries oceanography

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Genetic Polymorphism among Korean Salmonids Determined by RAPD (Randomly Amplified Polymorphic DNA) Analysis

  • Park, Jung-Youn;Kim, Mi-Jung
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.12 no.2
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    • pp.102-111
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    • 2007
  • RAPD analyses using 60 OPERON primers and 13 URPs were performed in order to assess the genetic variation and frequency of polymorphisms in Korean salmonids. RAPDS were very reproducible and most useful at the sub-species level. In RAPD analysis, 138 polymorphic bands were detected between Oncorhynchus masou subspecies and 99 bands were generated in two types of rainbow trout. Estimated genetic distances between O. masou subspecies were 0.28794, and between wild rainbow trout and an albino mutant was 0.22786. Each species of salmonid was well characterized using URP 4R, the obtained bands could be useful as a species specific RAPD markers.

Recognize Survey for Founding of the Department of Marine Leisure Industry in Fisheries High School (수산계고 해양레저산업과 신설에 대한 인식 조사)

  • Park, Jong-Un;Kim, Yeung-Hoon;Kang, Beodeul;Won, Hyo-Heon
    • Journal of Fisheries and Marine Sciences Education
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    • v.27 no.2
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    • pp.556-565
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    • 2015
  • The purpose of this study is to research on founding of the department of marine leisure industry in fisheries high school. Research methods were used a survey district, students and teachers in fisheries high school and industry human are 353 people in the target. The results of this research are as follows. First, the ratio in favor of making the marine leisure industrial department had more than 81%. The second, teachers and industry people selected core factors include oceanography, water leisure, leisure diving, commercial diving, lifeguard, rafting. Finally, subject for the department of marine leisure industry in fisheries high school are fisheries shipping industry basis, understanding of the ocean, marine leisure tourism, underwater technology, navigation, seamanship, maritime laws regulations, heat engines and aquatic organism.

First Record of Three Barracudina Fishes (Aulopiformes: Teleostei) in Korean Waters

  • Kim, Jin-Koo;Park, Jeong-Ho;Choi, Jung-Hwa;Choi, Kwang-Ho;Choi, Yeong-Min;Chang, Dae-Soo;Kim, Young-Seop
    • Ocean Science Journal
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    • v.42 no.2
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    • pp.61-67
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    • 2007
  • Three barracudina fishes (Paralepididae) were newly recorded from the southern sea of Korea. Lestidium prolixum is characterized by a single band of luminous ducts on the midventral line and absence of a papilla anterior to the eye. Lestrolepis intermedia and L. japonica are similar in having a black papilla immediately in front of the eye and two bands of luminous ducts on the midventral line. However, the dorsal fin of L. intermedia is located more posterior along the body than that of L. japonica, and L. intermedia have larger number of anal fin rays 42 (vs. 36-40) and vertebrae 95 (vs. 86-87) than L. japonica.

Characteristics of chain-forming cells in Gyrodinium impudicum using fluorescent ConA

  • Park, Eun-Ju;Kim, Gwang-Hoon;Cho, Yong-Chul;Cho, Eun-Seob
    • Journal of the korean society of oceanography
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    • v.34 no.4
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    • pp.214-219
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    • 1999
  • Cells of the dinoflagellate Gyrodinium impudicum form characteristic chains, which are associated with sugar accumulated on the cell surface. To resolve the relationship between chain-formation and cell surface sugar accumulation, confocal microscopy was used to observe sugar accumulation points in the vegetative cells and long chain-forming cells of G. impudicum cells treated with fluorescent-tagged ConA. In the stationary and exponential phases, both vegetative cells and chain-forming cells were similar to each other in fluorescent intensity. There was no evidence that chain-forming cells had a specific location for sugar accumulation on the cell surface. Most of the cells formed 2-cell chains one day after inoculation, but longer chains consisting of 4-8 cells increased markedly in 4day and 8 day cultures. Gyrodinium impudicum chains usually consist of more than four cells, and chains of 8 or even 16 cells can be observed in mature cultures. Temperature played an importantrole in chain-formation of the cells, threshold temperature for the development of long chain-formation was 19 $^{\circ}$C.

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The Rapid Differentiation of Toxic Alexandrium and Pseudo-nitzschia Species Using Fluorescent Lectin Probes

  • Cho, Eun-Seob;Park, Jong-Gyu;Kim, Hak-Gyoon;Kim, Chang-Hoon;Rhodes, Lesley L.;Chung, Chang-Soo
    • Journal of the korean society of oceanography
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    • v.34 no.3
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    • pp.167-171
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    • 1999
  • Since toxic Alexandrium catenella and non-toxic A. fraterculus are morphologically similar, they are difficult to discriminate under the light microscope. However, a novel technology, such as fluorescein isothiocyanate (FITC)-conjugated lectin probes enables easy and rapid differentiation. Toxic A. catenella bound seven different lectins, whereas the non-toxic A. fratercuzus did not bind Arachis hypogaea (PNA) lectin. In addition, Pseudo-nitrschia species in this study were also difficult to identify to species level with light microscope techniques, but it was possible to classify them using fluorescent lectins. Pseudo-nitzschia multistriata, P. subfraudulenta and P. pungens bound Canavalia ensiformis (ConA), whereas P. subpaclfica did not, and P. pungens also bound Ricinus communis (RCA). These results imply that lectin could be used as a critical tool in the differentiation of P. multistriata, P. subfraudulenta and P. pungens. However, P. subpacifica was not differentiated by the lectins tested. Therefore, it isconcluded that lectin probes are useful for discriminating toxic A. catenella from non-toxic A. fraterculus, and for the identification of some Pseudo-nitzschia species. In addition, this method has a great potential to speed and detection between non-toxic and toxic harmful algal blooms (HABs) in Korean biotoxin monitoring systems.

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Species Composition and Behavioral Characteristics of Released Black Rockfish, Sebastes inermis in the Coastal Waters off Namhae Island, Korea (남해도 주변해역에서 서식하는 어류의 종조성 및 양식산 방류 볼락(Sebastes inermis)의 행동특성)

  • An, Cheul-Min;Kwak, Seok-Nam;Park, Joo-Myun;Huh, Sung-Hoi
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.3
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    • pp.262-269
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    • 2010
  • Seventeen fish species were counted in an underwater visual census, and the most common species, Sebastes inermis and Halichoeres poecilopterus, were released. More than 60% of the released S.inermis occurred near a fish farm 5 days after release, but they moved to a distant rocky area, and decreasing numbers were seen near the farm 90 days after release. In pots, a total of 722 individuals belonging to 43 species occurred, and released S.inermis dominated with 174 individuals recaptured during the study period. The other common fish species were Stephanolepis cirrhifer and Acanthopagrus schlegeli. The percentage of recapture was 0.23%, but declined to 0.12% at 90 days after release. The potential predators of released S.inermis included Lateolabrax japonicus and Sebastes schlegeli.

Isotopic Determination of Terrestrial Food Sources for a Brackish Water Clam Corbicula japonica PRIME in an Estuarine System of Youngil Bay, Korea

  • Lee, Won-Chan;Park, Jin-Il;Choi, Woo-Jeung;Kim, Young-Seop;Lee, Pil-Yong;Kang, Chang-Keun
    • Journal of the korean society of oceanography
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    • v.35 no.1
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    • pp.56-64
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    • 2000
  • The importance of terrestrial organic matter as a food source for a brackish water clam Corbicula japonica was evaluated using stable carbon isotope ratios (${\delta}^{13}$C) in its tissues and potential food resources in an estuarine system of Youngil Bay, Korea. Suspended particulate organic matter (POM) had distinct ${\delta}^{13}$C values from riverine (-31.8 to -27.2%$_o$) to marine waters (-21.0 to -16.6%$_o$). Estuarine macroalgae had a wide ${\delta}^{13}$C range of -22.8 to -15.0%$_o$. The ${\delta}^{13}$C values of riverine POM were more negative than that of riverine phytoplankton (-26.5 to -24.2%$_o$) but similar to that of freshmarsh plant species (-29.1 to -27.5%$_o$ for Phragmites communis and -28.5 to -27.0%$_o$ for Salix gracilistyla), These ${\delta}^{13}$C values suggest that the POM transported by the Hyungsan River is predominantly of terrestrial origin rather than riverine autochthonous sources. The ${\delta}^{13}$C values of Corbicula japonica tissues (-28.7 to -27.2%$_o$) were most similar to values for riverine POM and freshmarsh plants. There was no significant difference in the isotopic composition of the clam individuals. The results indicate a predominant contribution of organic carbon derived from terrestrial and fresmarsh plant detritus to the diet of Corbicula japonica. Our results also confirm previous suggestion that terrestrial organic matter can be incorporated into estuarine food webs although its role is confined to the upper estuarine reaches.

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Distributions of East Asia and Philippines ribotypes of Cochlodinium polykrikoides (Dinophyceae) in the South Sea, Korea (Cochlodinium polykrikoides (Dinophyceae)의 동아시아와 필리핀 유전형의 남해안 분포)

  • PARK, TAE GYU;KIM, JIN JOO;SONG, SEON YOUNG
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.24 no.3
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    • pp.422-428
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    • 2019
  • Fish killing dinoflagellate Cochlodinium polykrikoides has been separated into four genetically differentiated subpopulations globally based on large-subunit (LSU) ribosomal RNA gene, and two subpopulations have been found in the South Sea, Korea. In this study, distributions of the East Asia and Philippines ribotypes were surveyed in the South Sea for 3 years (2014~2016) using quantitative real-time PCR (qPCR). The East Asia ribotype was detected in all sampling stations of the South Sea (Tongyeong~Wando) by 40~100% positives for 2014~2016, whereas the Philippines ribotype was detected in some areas of Tongyeong~Goheung by 1~2% positives for only 2016 when the Tsushima Warm Current (TWC) was particularly strengthened. These results indicate that the East Asia ribotype is the dominant subpopulation in the South Sea, also some of C. polykrikoides swimming cells might be transported from offshore to the South Sea via TWC.

Pollution of Heavy Metals and Sedimentation Rates in Sediment Cores from the Chinhae Bay, Korea (진해만 퇴적물의 퇴적속도와 중금속 오염)

  • Yang, Han-Soeb;kim, Seong-Soo;Kim, Gue-Buem
    • Journal of Environmental Science International
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    • v.4 no.5
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    • pp.103-103
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    • 1995
  • In the Chinhae Bay, Korea, sedimentation rates and sedimentary record of anthropogenic metal loads were determined by $^{210}Pb$ dating and heavy metal analysis of four sediment cores . The sedimentation rates varied from 0.16g/$cm^2$/yr(3.1mm/yr) at Sta. Ct, located within narrow waterway to 0.24g/$cm^2$/yr(4.8mm/yr) at Sta. Cl, located in Haengam Bay. Maximum contents of Mn, Zn, Cu and Cr were observed at Sta. C2 located near the mouth of Masan Bay, while minimum contents were observed at Sta. CB. Mn/Fe ratios at Sta. C2 and Sta. C4 showed gradually increasing and decreasing downward, respectively, in the upper layer of sediment cores. This suggests that Mn may be diagenetically redistributed in highly reduced environment. At Sta. C2, the concentrations of Zn and Cu began to increase from 1920s by anthropogenic input and have been remarkablely increasing since mid 1960s. At Sta. C3, located near Sungpo, anthropogenic input of these two elements has also slightly increased after 1970s. However, pollution of these two elements was not significant in Haengam Bay(Sta. Cl) and Chiljun watenway(Sta. C4). The pollution of Co, Ni and Cr was not remarkable in all core samples except surface sediment of Sta. C2. The total input of anthropogenic Zn and Cu since 1920s was estimated to be 28∼792 ㎍/cm2 and 0∼168㎍/cm2, respectively. Sta. C2 showed remarkablely higher values relative to other stations: anthropogenic loads of Zn and Cu constituted 27% and 29% of the total sedimentary inventories at the present day, respectively. Fe, Ni, Cr and Co contents showed good correlation(r>0.8) with each other. Anthropogenic Zn and Cu also showed a very good positive correlation(>0.9). However, correlation between these two group of element was quite scattered, indicating different sources and geochemical behaviors.