• Title/Summary/Keyword: Fish-eye

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First Morphological Description of a Larval Sleek Unicornfish Naso hexacanthus(Acanthuridae, Perciformes) Identified by COI Barcoding in the East China Sea (COI 바코딩으로 동정한 남방표문쥐치(Naso hexacanthus) 치어의 첫 형태 기재)

  • Choi, Hae-young;Choi, Hee-chan;Kim, Sung;Oh, Hyun-ju;Youn, Seok-hyun
    • Korean Journal of Ichthyology
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    • v.34 no.2
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    • pp.119-126
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    • 2022
  • Here, we present the first morphological description of a larval Naso hexacanthus (5.2 mm in body length) from the East China Sea identified by cytochrome c oxidase subunit I (COI) barcoding. The larva had a kite-shaped body with long serrated first spine of dorsal and anal fins. There were four melanophores on the base of the anal fin, dense melanophores on the caudal peduncle, and scattered melanophores on the surface of the brain. There was one small spine on the snout and behind each eye, with serrations on the head, top of the eye, inner- and outer-preopercle, and on the lower part and side of the opercle. The morphological characteristics of larval N. hexacanthus identified by COI barcoding will be useful for species identification of larval fish.

cDNA Cloning and Expression of a Cytochrome P450 1A (CYP1A) from the Pale Chub, Zacco platypus

  • Jeon, Hyoung-Joo;Park, Young-Chul;Lee, Wan-Ok;Lee, Jong-Ha;Kim, Jin-Hyoung
    • Korean Journal of Ecology and Environment
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    • v.44 no.4
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    • pp.364-372
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    • 2011
  • The pale chub (Zacco platypus) is generally found in Asian countries, such as Korea, Japan, and China. Nevertheless, very little information exists about the genes involved in the metabolism of xenobiotics in this species. This species is useful in monitoring the environmental impact on various pollutants in freshwater as a sentinel fish species. We cloned the full-length cDNA sequence of xenobiotic metabolizing cytochrome P450 1A (CYP1A) gene from Z. platypus and characterized it. Tissue distribution and timedependent induction of CYP1A were studied by real-time RT-PCR. Induction pattern of CYP1A was studied by exposing the fish to an arylhydrocarbon receptor agonist, ${\beta}$-naphthoflavone (BNF). The liver showed the highest level of expression in basal state as well as BNF- treated fish. However, appreciable levels of expression were also recorded in Gill and kidney and the least level of expression was observed in the eye. The results of the time-course study revealed an induction in the liver, brain, and gills after 6 h and 12 h in most of the tissues. This study provides an insight into the xenobiotics metabolizing system of Z. platypus and offers baseline information for further research related to biomarker, stress, and adaptive response of this ecologically important fish species in the freshwater environment.

Studies on the Morphological Characteristics and Life History of the Chili Fish, Cultriculus eigenmanni (Jordan et Metz) (1) On the Egg Development and the Fry in the Larval Stage (치리(Cultriculus eigenmanni)의 형태 및 생활사에 관한 연구 I. 난발생과 부화자어에 대하여)

  • KIM Eul-Bae;KIM Young-Gill
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.17 no.2
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    • pp.151-156
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    • 1984
  • The Chili fish, one of the most common species of the freshwater fishes, is distributed in the river basins of the western coast of Korea. However, any studies on this fish, especially its development or life history, have not been made so far. The authors, therefore, carried out an experiment to reveal some characteristics of egg develoment and fry in this fish. For accomplshing this study, we caught some adult Chili fish by means of a casting net at raceway in the vicinity of Kunsan in June and July, 1983; then, we injected 0. 1 cc Gonadotrophic hormone 1,000 unit solution (Puberogen) in order to induce the spawning on the abdominal cavity of male and female respectively, Approximately 12 hours after injection, the artificially fertilized eggs by using of the dry method and the naturally fertilized eggs were observed simultaneously in the aquarium ($30{\times}50{\times}70cm$). The fertilized eggs, in spherical shape, were sperative, demersel, adhesive nature and $1.38{\sim}1.59\;mm$ in diameter. The light yellow yolk was opaque and $1.17{\sim}l.27\;mm$ in diameter. The hatching of eggs took place at 29.5 hours after fertilization under the condition of $23.5{\sim}25.0^{\circ}C$ water temperature. Basically there were not found so many different aspects in comparison with the common species of Cyprinidae in the development process of eggs : however, there were a few noticable characteristics, that is, pigment cells, eye balls and blood circulation are invisible, or the velocity of egg development is rapid, seemed similar to the Loach's egg. Newly hatched larvae with the yolk sac (2.39 mm in size) were $3.87{\sim}3.99\;mm$ in total length and their myotome number was expressed as 28+15=43. About 20 hours after hatching, a caudal and a pectoral fin as well as an anus began to orgin. In addition, a few malanophore showed on the upper regin of the yolk sac of the larvae whose size was 4.73 mm in total length. The larvae developed about 5.02 mm in total length, and the median fin fold in the abdomen extended forward to two thirds or beyond of the yolk sac at 50 hours after hatching. Simultaneously, the eye balls and mouth also appeared. After 3 days, the yolk sac nearly disappeared, and the larvae (5.4 mm in total length) with perfect eye balls began to swim for themselves for the first time. Also, the specialization between the gill arches and the ailmentary canal, the development of each fins except dorsal fin as well as the blood circulation obviously distinguished in this stage. The larvae whose total length was 5.68 mm was very brisk: also, the development of the dorsal fin rudiment, nearly perfect formation of each organs and the function of the upper and lower jaws were noticeably observed 5 days after hatching.

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Early Growth and Characteristic of Histological Eye Development in Post Parturition Dark banded Rockfish, Sebastes inermis (볼락, Sebastes inermis 산출 후 초기 성장 및 눈의 조직학적 발달 특성)

  • Park, In-Seok;Park, Hye-Jung;Gil, Hyun-Woo;Goo, In-Bon
    • Development and Reproduction
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    • v.16 no.2
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    • pp.101-106
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    • 2012
  • Importance of behavior factors or environmental factors in visual organization and visual function of fish is treated with great care in visual ecology, and there is no study about initial ocular growth and development on the dark banded rockfish, Sebastes inermis. Thus, this study was performed. The total length, head length, head depth, eye diameter and lens diameter of the dark banded rockfish showed positive allometric relationship between parturition stage and 60 days post-parturition (dpp). The increase in total length relative to head length and head depth, head length growth relative to eye diameter and lens diameter, and head depth growth relative to eye and lens diameter were nearly isometric. The eyes were formed completely at parturtion stage. At this age, the eye has an optic nerve fiber layer, a ganglion cell layer, an inner plexiform layer, an inner nuclear layer, an outer plexiform layer, an outer nuclear layer, an outer limiting membrane, a rod and cone layer and an epithelial layer. Thickness of retina at 60 dpp was higher than that of at parturition stage. During this experiment, the proportion of the rod and cone layer, outer nuclear layer, and optic nerve fiber layer of retina were significantly increased, while the proportion of the outer plexiform layer, inner nuclear layer and ganglion cell layer of retina were significantly decreased (P<0.05). The essential demands that must be met by the retina in this species pertain to light sensitivity and spatial resolution.

Interspecific hybridization in seahorses: artificially produced hybrid offspring of Hippocampus kuda and Hippocampus reidi

  • Han, Sang-Yun;Rho, Sum;Noh, Gyeong Eon;Kim, Jin-Koo
    • Fisheries and Aquatic Sciences
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    • v.21 no.5
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    • pp.11.1-11.8
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    • 2018
  • Interspecific hybridization experiments were conducted between the common seahorse Hippocampus kuda (male) and the slender seahorse H. reidi (female) during artificial rearing to develop a new aquarium fish with unique polyandrous mating. Molecular analysis via mitochondrial DNA (mtDNA) cytochrome b and nuclear DNA (ncDNA) ribosomal protein S7 gene supported the hybridization between the two species, and the hybrid also showed morphological characteristics of both species. Juveniles of H. kuda have dense melanophores on the whole body or only on the trunk and tail, whereas juveniles of H. reidi have thin melanophores on the whole body or present in stripes only along their prominent trunk and tail rings. However, all the hybrid juveniles had dense melanophores only on the tail, with the striped trunk rings, thus showing an intermediate pattern, and these patterns were limited to the fairly early stage of development (1-10 days old). In contrast, the two eye spines in the hybrid were apparent after 9 days old, which were not inherited from H. kuda (one eye spine), but from H. reidi (two eye spines). According to LOESS (local regression) analysis, the growth rate increased between 20 and 25 days, and the hybrids grew faster than H. kuda when they entered the explosive second phase of growth between 25 and 45 days for all the seahorses. This study highlights the hybridization between H. kuda and H. reidi may contribute to the improved taxonomic information of young seahorses.

Three Unrecorded Marine Fish Species from Korean Waters

  • Park, Jeong-Ho;Kim, Jin-Koo;Moon, Jee-Hwan;Kim, Cheol-Bum
    • Ocean Science Journal
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    • v.42 no.4
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    • pp.231-240
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    • 2007
  • Three marine fish species are recorded for the first time from Korean waters: a molid (Ranzania laevis, 1 specimen, 279.8 mm SL) and bramid (Pterycombus petersii, 3 specimens, 95.3-214.0 mm SL) collected from a large purse seine off Jeju Island, in the southern sea of Korea, and a carangid (Carangoides dinema, 1 specimen, 194.5 mm SL) from a set net in coastal waters off Busan, in the southeastern sea of korea. R. laevis is characterized by a wedge-shaped body and truncated clavus; P. petersii by the dorsal fin origin above or behind the posterior margin of eye, and dorsal and anal fins depressible; and C. dinema by a row of black blotches along the second dorsal fin base, the curved part of the lateral line longer than straight part, and 18 and 16 dorsal and anal fin rays, respectively. New Korean names are proposed for all three species.

First Record of Gymnogobius cylindricus (Perciformes: Gobiidae) from Incheon River Estuary, Jeonbuk-do, Korea (전북 인천강 하구역에서 출현한 망둑어과(농어목) 한국미기록종, Gymnogobius cylindricus)

  • Kim, Byung-Jik;Song, Ho-Bok;An, Jung-Hyun;Choi, Seung-Ho
    • Korean Journal of Ichthyology
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    • v.25 no.3
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    • pp.173-177
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    • 2013
  • Based on a single specimen (49.9 mm SL) collected from Incheon River estuary, south-western Korea, Gymnogobius cylindricus was described as the first record from Korea as well as the westernmost record of the world. The species is characterized by having a large maxilla extend far beyond posterior margin of eye, dorsal fin with VI-I, 12 rays, anal fin with I, 11 rays, and without dark blotches and dots on lower part of body and caudal fin, respectively. A new Korean name, "Ga-neunsal-mang-dug" is proposed for the species.

A Cyan Fluorescent Protein Gene (cfp)-Transgenic Marine Medaka Oryzias dancena with Potential Ornamental Applications

  • Vu, Nguyen Thanh;Cho, Young Sun;Lee, Sang Yoon;Kim, Dong Soo;Nam, Yoon Kwon
    • Fisheries and Aquatic Sciences
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    • v.17 no.4
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    • pp.479-486
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    • 2014
  • To evaluate their potential utility as an ornamental organism, novel transgenic marine medaka Oryzias dancena strains with a highly vivid fluorescent phenotype were established through transgenesis of a cyan fluorescent protein gene (cfp) driven by the endogenous fast skeletal myosin light chain 2 gene (mlc2f) promoter. The transgenic marine medaka strains possessed multiple copies of transgene integrants and passed their fluorescent transgenes successfully to subsequent generations. Transgenic expression in skeletal muscles at both the mRNA and phenotypic levels was, overall, dependent upon transgene copy numbers. In the external phenotype, an authentic fluorescent color was dominant in the skeletal muscles of the transgenic fish and clearly visible to the unaided eye. The phenotypic fluorescent color presented differentially in response to different light-irradiation sources; the transgenics displayed a yellow-green color under normal daylight or white room light conditions, a strong green-glowing fluorescence under ultraviolet light, and a cyan-like fluorescence under blue light from a light-emitting diode.

Molecular Cloning and Tissue-specific Expression of the Melanocortin 4 Receptor Gene from Olive Flounder, Paralichthys olivaceus

  • Lee, Hye-Jung;Kim, Jong-Myoung
    • Fisheries and Aquatic Sciences
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    • v.13 no.4
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    • pp.263-271
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    • 2010
  • G protein-coupled receptors (GPCR) constitute the largest superfamily of cell membrane receptors, mediating diverse signal-transduction pathways. The melanocortin 4 receptor (MC4R) has been of interest for its physiological role and size, one of the smallest among the GPCRs, which makes it a good model system for the structural study of GPCRs. To study the molecular structure and tissue-specific expression of MC4R in olive flounder (Paralichthys olivaceus), the full-length MC4R gene was obtained using PCR amplification of genomic DNA as well as cDNA synthesis. Sequence analysis of the gene indicates that 978 bp of the MC4R gene encodes 325 amino acids without introns. Sequence alignment with the MC4Rs from other fish shows the highest degree of identity (96%) between Paralichthys olivaceous and Verasper moseri, followed by Takifugu rubripes and Tetraodon nigroviridis (89%). RNA was isolated from various tissues to examine the tissue distribution of MC4R by using RT-PCR. The results showed major expression of MC4R in the liver, brain, and eye, which is consistent with the expression pattern in other fish belonging to the order Pleuronectiformes.

Electrophoretic Patterns of Sarcoplasmic Proteins in Mid-West Korean Sea Fishes by Thin Layer Polyacrylamide Gel Isoelectrofocusing (등전점 전기영동에 의한 서해산 해산어의 근형단백질의 전기영동에 관한 연구)

  • 김종배;최선남;이근우;정용현
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.5
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    • pp.455-460
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    • 1991
  • In order to identify hard distinct 12 fish species(shiba shrimp Metapenaeus joyneri, fleshy shrimp Penaeus orientalis, ridgetail prawn Palaemon carinicauda, yellow croaker Pseudosciaena manchurica, croaker Niber albiflora, Colichthyes fragilis, brown sole Limanda herzensteini, frog flounder Pleuronichthys cornutrs, Areliscus rhomaleus, stone flounder Kareius bicoloratus, harvest fish Pampus argenteus, flag fish Goniistius zonatus) by seeing with naked eye in Kunsan coastal area, sarcoplasmic protein in the supernatant was used for isoelectric focusing. For getting supernatant, fish muscle tissue was blended with two times deionized water and centrifuged (at $4^{\circ}C$, 12,000rpm for 15min). Isoelectric focusing of sarcoploasmic protein carried out on a LKB Multiphor II using polyacrylamide gel plate (2mm thickness, pH $3.5~10^{\circ}C$, pH 5~8 gradient, at $10^{\circ}C$ for 1.5, 3 hours). In case of uncertain protein pattern, pH gradient was modified to narrow pH gradiet, and excuted 2-D electrophoresis using conventional polyacrylamide gel electrophoresis. Most of fishes except yellow croaker and Collichthyes fragilis were distingushed by isoelectric focusing. The protein maps of 2-D electrophoresis for analyzing two protein bands at aimilar positions(pH 5, 6) between the two fish species showed the diffeences of the estimated molecular weights, 11,700(pH5.0) and 87,000(pH6.0)

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