• Asian-Australasian Journal of Animal Sciences
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• v.27 no.1
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• pp.62-68
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• 2014

This feeding trial was carried out to evaluate the effects of different dietary cadmium levels on growth and tissue cadmium content in juvenile parrotfish, Oplegnathus fasciatus, using cadmium chloride ($CdCl_2$) as the cadmium source. Fifteen fish averaging $5.5{\pm}0.06$ g (mean${\pm}$SD) were randomly distributed into each of twenty one rectangular fiber tanks of 30 L capacity. Each tank was then randomly assigned to one of three replicates of seven diets containing 0.30 ($C_0$), 21.0 ($C_{21}$), 40.7 ($C_{41}$), 83.5 ($C_{83}$), 162 ($C_{162}$), 1,387 ($C_{1,387}$) and 2,743 ($C_{2,743}$) mg cadmium/kg diet. At the end of sixteen weeks of feeding trial, weight gain (WG), specific growth rate (SGR) and feed efficiency (FE) of fish fed $C_{21}$ were significantly higher than those of fish fed $C_{83}$, $C_{162}$, $C_{1,387}$ and $C_{2,743}$ (p<0.05). Weight gain, SGR and FE of fish fed $C_0$, $C_{21}$ and $C_{41}$ were significantly higher than those of fish fed $C_{162}$, $C_{1,387}$ and $C_{2,743}$. Protein efficiency ratio of fish fed $C_0$, $C_{21}$ and $C_{41}$ were significantly higher than those of fish fed $C_{1,387}$ and $C_{2,743}$. Average survival of fish fed $C_0$, $C_{21}$, $C_{41}$ and $C_{162}$ were significantly higher than that of fish fed $C_{2,743}$. Tissue cadmium concentrations increased with cadmium content of diets. Cadmium accumulated the most in liver, followed by gill and then muscle. Muscle, gill and liver cadmium concentrations of fish fed $C_0$, $C_{21}$, $C_{41}$ and $C_{83}$ were significantly lower than those of fish fed $C_{162}$, $C_{1,387}$ and $C_{2,743}$. Based on the ANOVA results of growth performance and tissue cadmium concentrations the safe dietary cadmium level could be lower than 40.7 mg Cd/kg diet while the toxic level could be higher than 162 mg Cd/kg diet.

• The Journal of Information Systems
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• v.32 no.4
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• pp.137-147
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• 2023

Purpose The domestic aquaculture industry in South Korea utilizes both formulated feeds and live feeds for the cultivation of fish. While nutrient-rich live feeds, particularly using fry, have been preferred since the past, formulated feeds are gaining attention due to issues related to overfishing and environmental concerns. Formulated feeds are advantageous for storage and supply but require a sustained feeding regimen due to the comparatively slower growth rate compared to live feeds. As the aging population in rural areas leads to a shortage of labor, automated feeding systems are increasingly being adopted in aquaculture facilities. To enhance the efficiency of such systems, it is crucial to quantitatively analyze the behavioral changes in fish based on the presence or absence of feed. Design/methodology/approach In the study, RGB cameras and infrared cameras were used to analyze fish activity according to feeding, and an outline extraction algorithm was applied to analyze the differences resulting from this. Findings Unlike RGB cameras, infrared cameras are more suitable for analyzing underwater fish activity as they convert objects' thermal energy into images. It was observed that Canny, Sobel, and Prewitt filters showed the most distinct identification of fish activity.

• Fisheries and Aquatic Sciences
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• v.18 no.2
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• pp.143-149
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• 2015

This experiment was conducted to evaluate the synergistic effects of vitamin C and E on methylmercury (MeHg) toxicity in juvenile olive flounder Paralichthys olivaceus. In a $3{\times}3$ factorial design, 9 experimental diets containing three different vitamin C (0, 200 or 400 mg/kg diet in the form of l-ascorbyl-2-monophosphate) and vitamin E (0, 100 or 200 mg/kg diet in the form of dl-${\alpha}$-tocopheryl acetate) levels with the Hg toxicity level (20 mg/kg diet in the form of MeHg) were formulated. Triplicate groups of fish averaging $2.3{\pm}0.05g(mean{\pm}SD)$ were fed one of the 9 diets in a flow through system for 8 weeks. Fish fed 400 mg vitamin C/kg diet with 100 or 200 mg vitamin E/kg diet showed significantly (P < 0.05) higher weight gain (WG) than did fish fed the other diets. Fish fed 400 mg vitamin C/kg diet at all vitamin E levels and those which fed vitamin C and E equally at a rate of 200 mg/kg diet showed significantly (P < 0.05) higher feed efficiency (FE), specific growth rate (SGR) and protein efficiency ratio (PER) than did fish fed the other diets. Fish fed 200 and 400 mg vitamin C/kg diet exhibited significantly (P < 0.05) lower Hg concentration in their muscle as well as kidney than did fish fed the other diets. Therefore, these results clearly indicated that the synergistic effects of these two vitamins on MeHg toxicity by supplementing dietary vitamin C (200 and 400 mg/kg diet) with vitamin E (100 and 200 mg/kg diet) in juvenile olive flounder.

• Fisheries and Aquatic Sciences
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• v.19 no.8
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• pp.32.1-32.6
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• 2016

A feeding trial was conducted to evaluate the effects of two different sizes of extruded pellets (EP) ($EP_1$ - 3 mm or $EP_2$ - 5 mm) and a moist pellet (MP) in olive flounder, Paralichthys olivaceus, reared in semi-recirculation system. A total of 450 fish with an average initial weight of $5.0{\pm}0.2g$ (mean ${\pm}$ SD) were fed one of the three experimental diets in triplicate groups. At the end of a 6-week feeding trial, weight gain, specific growth rate, and feed efficiency of fish fed EP diets were significantly higher than those of fish fed MP (P < 0.05). Water quality parameters like turbidity, total ammonia nitrogen, and total phosphorous from tanks of fish fed $EP_1$ and $EP_2$ were significantly lower than those from tanks of fish fed MP. Blood plasma glutamic oxaloacetic transaminase and glucose concentration were significantly higher in fish fed MP diet compared to fish fed EP diets (P < 0.05). Whole body crude protein contents in fish fed EP diets were higher than those from the fish fed MP diet. Whole body amino acid content like threonine, aspartic acid, serine, tyrosine, and cystine were found to be significantly higher in fish fed EP diets than those in fish fed MP diet. In considering overall performance of olive flounder, $EP_2$ diet could be recommended for the successful aquaculture of this important fish species.

• Fisheries and Aquatic Sciences
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• v.22 no.1
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• pp.1.1-1.8
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• 2019

Five experimental diets were formulated to evaluate the effects of dietary enzymatically hydrolyzed tuna by-product on growth, non-specific immune responses, and hematology of juvenile rainbow trout (Oncorhynchus mykiss). A basal diet with 50% of fishmeal was used as control (CON) and four other diets replaced 12.5% ($TBB_{12.5}$), 25% ($TBB_{25}$), 37.5% ($TBB_{37.5}$), and 50% ($TBB_{50}$) of fish meal in the CON diet. Juvenile rainbow trout ($4.87{\pm}0.05g$) were randomly distributed into 15 tanks (50 L) and fed 3-4% of wet body weight two times a day. At the end of 7 weeks of feeding trial, weight gain, specific growth rate, feed efficiency, and protein efficiency ratio of fish fed CON diet were significantly higher than those of fish fed $TB_{50}$ diet (P < 0.05). But there were no significant differences among fish fed CON, $TBB_{12.5}$, $TBB_{25}$, and $TBB_{37.5}$ diets (P > 0.05). There were no significant differences in GPT levels among fish fed CON, $TBB_{12.5}$, $TBB_{25}$, and $TBB_{37.5}$ diets. Also, there were no significant differences in lysozyme, superoxide dismutase, glucose, and total protein levels in all experimental diet (P > 0.05). The broken-line analysis indicated that the minimum dietary level of enzymatically hydrolyzed tuna by-product to replace fishmeal could be 29.7% in rainbow trout. These results indicated that the optimum level of dietary enzymatically hydrolyzed tuna by-product could replace greater than 29.7% but less than 37.5% of fishmeal in juvenile rainbow trout diet.

• Journal of Veterinary Clinics
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• v.19 no.1
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• pp.1-6
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• 2002

We evaluated the wholesomeness of fish feeds treated with high-dose radiation. This study examined the safety of fish fed treated with high-dose irradiation (5 kGy) and butylated hydroxyanisole (BHA, 200 ppm). 100 flounder (Paralichthys olivaceus) were grouped into 4 and then the fishes fed the following feeds for 28 days: (1)standard feed; (2)standard feed, treated with BHA; (3)standard feed, irradiated to 5 kGy; (4)standard feed, treated with BHA and irradiated to 5 kGy. Four groups each 10 mice were fed the fish feeds same as (1)-(4) for 14 days. There were no observable differences between fishs fed the irradiated feed and those fed the standard feed with respect to growth, feed consumption, haematology and organ histopathology Parameters. There were no deviations in feed consumtion, haematology and organ histopathology parameters in the experiment of mice. We concluded that there were no irradiation-related effects.

• Fisheries and Aquatic Sciences
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• v.17 no.2
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• pp.255-261
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• 2014

An 8-week feeding trial was conducted to investigate the effects of dietary taurine supplementation on the growth performance of juvenile rock bream, Oplegnathus fasciatus. Triplicate groups of 25 fish averaging $2.72{\pm}0.04$ g ($mean{\pm}SD$) were fed one of six experimental diets prepared by adding taurine at 0%, 0.25%, 0.5%, 1.0%, 1.5% and 3.0% (Control, $Tau_{0.25}$, $Tau_{0.5}$, $Tau_{1.0}$, $Tau_{1.5}$ and $Tau_{3.0}$, respectively). At the end of the feeding trial, the weight gain, specific growth rate and protein efficiency ratio of fish fed the $Tau_{0.5}$, $Tau_{1.0}$ and $Tau_{1.5}$ diets were significantly higher (P < 0.05) than those of fish fed the Control and $Tau_{0.25}$ diets. The feed efficiency of fish fed the $Tau_{0.5}$ diet was significantly higher than that of fish fed the Control, $Tau_{0.25}$ and $Tau_{3.0}$ diets. Fish fed the $Tau_{1.0}$ diet had higher whole-body crude protein content than fish fed the Control diet, while the crude lipid content of fish fed the $Tau_{1.5}$ and $Tau_{3.0}$ diets was significantly lower than that of fish fed the Control and $Tau_{0.25}$ diets. An ANOVA suggested that the optimum level of dietary taurine supplementation to improve growth and reduce the body lipid contents of juvenile rock bream, O. fasciatus, was 0.5%, while a broken line analysis of weight gain indicated a level of 0.62%.

• Fisheries and Aquatic Sciences
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• v.19 no.5
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• pp.24.1-24.6
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• 2016

We determined the optimum dietary protein level in juvenile barred knifejaw Oplegnathus fasciatus in cages. Five semi-purified isocaloric diets were formulated with white fish meal and casein-based diets to contain 35, 40, 45, 50, and 60 % crude protein (CP). Fish with an initial body weight of $7.1{\pm}0.06g$ ($mean{\pm}SD$) were randomly distributed into 15 net cages (each size: $60cm{\times}40cm{\times}90cm$, $W{\times}L{\times}H$) as groups of 20 fish in triplicates. The fish were fed at apparent satiation level twice a day. After 8 weeks of feeding, the weight gain (WG) of fish fed 45, 50, and 60 % CP diets were significantly higher than those of fish fed 35 and 40 % CP diets. However, there were no significant differences in WG among fish fed 45, 50, and 60 % CP diets. Generally, feed efficiency (FE) and specific growth rate (SGR) showed a similar trend as WG. However, the protein efficiency ratio (PER) was inversely related to dietary protein levels. Energy retention efficiency increased with the increase of dietary protein levels by protein sparing from non-protein energy sources. Blood hematocrit content was not affected by dietary protein levels. However, a significantly lower amount of hemoglobin was found in fish fed 35 % CP than in fish fed 40, 45, 50, and 60 % CP diets. Fish fed 60 % CP showed the lowest survival rate than the fish fed 35, 40, 45, and 50 % CP diets. Broken-line analysis of WG showed the optimum dietary protein level was 45.2 % with 18.8 kJ/g diet for juvenile barred knifejaw. This study has potential implication for the successful cage culture of barred knifejaw.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2002.05a
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• pp.257-258
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• 2002

• Proceedings of the Korean Aquaculture Society Conference
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• 2002.08a
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• pp.232-234
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• 2002