• Title/Summary/Keyword: Fish Plate

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Electrophoretic Patterns of Sarcoplasmic Proteins in Mid-West Korean Sea Fishes by Thin Layer Polyacrylamide Gel Isoelectrofocusing (등전점 전기영동에 의한 서해산 해산어의 근형단백질의 전기영동에 관한 연구)

  • 김종배;최선남;이근우;정용현
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.5
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    • pp.455-460
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    • 1991
  • In order to identify hard distinct 12 fish species(shiba shrimp Metapenaeus joyneri, fleshy shrimp Penaeus orientalis, ridgetail prawn Palaemon carinicauda, yellow croaker Pseudosciaena manchurica, croaker Niber albiflora, Colichthyes fragilis, brown sole Limanda herzensteini, frog flounder Pleuronichthys cornutrs, Areliscus rhomaleus, stone flounder Kareius bicoloratus, harvest fish Pampus argenteus, flag fish Goniistius zonatus) by seeing with naked eye in Kunsan coastal area, sarcoplasmic protein in the supernatant was used for isoelectric focusing. For getting supernatant, fish muscle tissue was blended with two times deionized water and centrifuged (at $4^{\circ}C$, 12,000rpm for 15min). Isoelectric focusing of sarcoploasmic protein carried out on a LKB Multiphor II using polyacrylamide gel plate (2mm thickness, pH $3.5~10^{\circ}C$, pH 5~8 gradient, at $10^{\circ}C$ for 1.5, 3 hours). In case of uncertain protein pattern, pH gradient was modified to narrow pH gradiet, and excuted 2-D electrophoresis using conventional polyacrylamide gel electrophoresis. Most of fishes except yellow croaker and Collichthyes fragilis were distingushed by isoelectric focusing. The protein maps of 2-D electrophoresis for analyzing two protein bands at aimilar positions(pH 5, 6) between the two fish species showed the diffeences of the estimated molecular weights, 11,700(pH5.0) and 87,000(pH6.0)

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The Ammonia Removal Capacity of a Few Kinds of Filter Media in a Water Reuse quaculture System (순환 여과식 양식 시설에 이용될 수종의 여과 재료의 효능에 관한 연구)

  • KIM In-Bae;KIM Pyong-Kih;CHEE Young-Ok
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.20 no.6
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    • pp.561-567
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    • 1987
  • The purpose of this study was to find out the removal capacity of harmful ammonia by different filter media in the submerged biological filters in a given space of chamber. Four materials, pile cloth, corrugated skylight roofing plate, embossed plastic plate, and gravel, were used as the experimental filter media. Each filter medium was placed in two aquariums, each aquarium measuring $90cm\times60cm\times60cm\;(depth)$. Under the normal operating condition, the average of mean ammonia removal rates during the first and second functioning periods by each filter material which occupied tile space in the filter chamber (aquarium) was as follows: 1. Pile cloth: $8.381\;g{\cdot}m^{-3}.\;day^{-1}$ 2. Corrugated skylight roofing plate: $7.834\;g{\cdot}m^{-3}.\;day^{-1}$ 3. Embossed plastic plate: $7.797\;g{\cdot}m^{-3}.\;day^{-1}$ 4. Gravel: $7.051\;g{\cdot}m^{-3}.\;day^{-1}$ Thus, there were no significant differences between the media, but at the time of practical application of these materials, some other factors such as investment cost, easiness for the removal of excess detritus accumulated in tile interstices of filter media, etc. should be fallen into consideration. When large units are required, in particular, removal of excess detritus from tile gravel bed is extremely difficult, and in case of pile cloth filters the installation work is much complicated and a problem in supporting the structure when drained also exists. In these respects, corrugated skylight roofing plate and embossed plastic plate seem to be more optimal, but again in practice the local situation for the availability and the price of the materials should be rechecked and the fitness of tile materials in the particular filter chambers under use or under consideration for construction must be taken into account.

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An Analytical Study on the Youngjeob Dogam Zabmulsek Euigwae of Choson Dynasty (1609, 1643 year) (조선왕조(朝鮮王朝)의 영접도감(迎接都監) 잡물색의궤(雜物色儀軌)에 관한 분석적(分析的) 연구(硏究) 식품(食品)의 재료(材料)와 소용기용(所用器用)에 관하여 (1609년(年), 1643년(年)의 의궤(儀軌)를 중심(中心)으로))

  • Kim, Sang-Bo;Lee, Sung-Woo
    • Journal of the Korean Society of Food Culture
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    • v.7 no.2
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    • pp.119-125
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    • 1992
  • To analyze food material and table wares in daily reception dishes of Choson Dynasty, studied historic book 'Young jeob Dogam Zabmulsek Euigwae' (1609, 1643 year) described the daily reception dishes for Chinese envoy in Choson Dynasty. The results obtained from this study are as follows. 1. Food material for reception Chinese envoy were vegetable, pickled vegetable, soup (?水), brown seaweed, kelp, green seaweed, garlic, pine mushroom, mushroom, driedfish, fish egg, shrimp, pork, pheasant, fowl, salted fish shrimp and etc, various fruits preserved in honey, green peajelly, buck wheat jelly and bean curd. 2. The table wares were rice bowl(鉢里), small bowl of porcelain(甫兒), water bowl(大貼), plate(貼是), small dish(鍾子), small earthen ware jar(東海), jar(缸), pottery(甕), table(盤), chopsticks(?), washbowl(洗面盆), earthen ware steamer(甑), kettle(釜), brazier(爐), measuring cup(升), unit of measure(合), meter(稱子), spoon(匙), chest (函), lid(蓋), large rice bowl(所羅) and bowl(椀).

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An Analytical Study on the Youngjeob Dogam Bansunsek Euigwae of Choson Dynasty (1609, 1626, 1634 year) (조선왕조(朝鮮王朝)의 영접도감(迎接都監) 반선색의궤(盤膳色儀軌)에 관한 분석적(分析的) 연구(硏究) (1609년(年), 1626년(年), 1634년(年)의 의궤(儀軌)를 중심(中心)으로))

  • Kim, Sang-Bo;Lee, Sung-Woo
    • Journal of the Korean Society of Food Culture
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    • v.6 no.4
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    • pp.433-443
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    • 1991
  • To analyse reception dishes of Choson Dynasty the author studied historic book-Youngjeob Dogam Bansunsek Euigwae (1609, 1626, 1634 year) in which the daily reception dishes for Chinese envoy in Choson Dynasty were described. The results obtained from this study were as follows. 1. The daily food items for reception of Chinese envoy were refined rice wine, rice, large fish, fish, Zogi, Driedjogi, mullet, pork, beef, chicken, egg, salt, soysauce, sweet soy sauce, refined soy sauce, sesame oil, vinegar, bean curd, stone leek, parsley, ginger and gaslic etc. 2. The daily tablewares used for reception of Chinese envoy were ricebowl (bari), small bowl of porcelain (boa), water bowl (daejeop), soup bowl (tang gi), plate (jeopsi), small dish (jong gi), small earthen ware jar (dong hae), jar (hang), pottery (ong gi), wine glass (jan), water cup, chopsticks, kettle, cauldron, bottle, table, knife, napkin, dish towel, brazier, washbowl and meter etc.

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Degradation Characteristics of A Novel Multi-Enzyme-Possessing Bacillus licheniformis TK3-Y Strain for the Treatment of High-Salinity Fish Wastes and Green Seaweeds

  • Kang, Kyeong Hwan;Kim, Joong Kyun
    • Fisheries and Aquatic Sciences
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    • v.18 no.4
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    • pp.349-357
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    • 2015
  • To reutilize fisheries waste, we isolated a bacterial strain from a coastal area located in Busan. It was identified as Bacillus licheniformis TK3-Y. Using plate assay and 500-mL flask experiments, we found that the isolate simultaneously possessed cellulolytic, proteolytic, and lipolytic activities with salt tolerance. 10% (v/v) inoculums, were used to examine the biodegradation characteristics of the TK3-Y strain on carboxymethylcellulose, skim milk, and olive oil media. The optimum conditions for pH, temperature, agitation speed, and NaCl concentration on each 1% substrate were 6, $50^{\circ}C$, 180 rpm, and 17.5%, respectively. Under optimal conditions, the TK3-Y strain showed 1.07 U/mL cellulolytic, 1,426 U/mL proteolytic, and 6.45 U/mL lipolytic activities. Each enzyme was stable within a range of 17.5-35% NaCl. Therefore, the salt tolerance ability of strain TK3-Y was superior to other related strains. In degradation of a mixed medium containing all three substrates, both the cellulolytic and proteolytic activities were somewhat lower than those on each single substrate, while the lipolytic activity was somewhat higher. From the above results, the TK3-Y strain appears to be a good candidate for use in the efficient treatment of fisheries waste in which components are not collected separately.

Parasitism of Pseudocaligus sp. in wild grass puffer Takifugu niphobles, and their treatment (자연산 복섬에서의 Pseudocaligus sp. 기생과 그 구제에 대해)

  • Seo, Jang-Woo;Kim, Wi-Sik;Han, Kyeong-Ho;Lee, Sung-Hoon;Yoo, Dong-Jae;Kim, Yi-Cheong;Oh, Myung-Joo
    • Journal of fish pathology
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    • v.23 no.1
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    • pp.107-112
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    • 2010
  • Parasitism of Pseudocaligus sp. in wild grass puffer Takifugu niphobles in the coast of Yeosu in spring, 2007 was identified. The preadult and adult stages of Pseudocaligus sp. were observed, which consisted of cephalothorax, genital segment and abdomen. Lunules were present on the anterior margin of the frontal plate. In order to develop the treatment method, we examined the effects of formalin and fresh water on the parasite. The Pseudocaligus sp. were detached from the host fish by bathing at concentrations of 50 to 400 ppm formalin within 60 min, but 57% was exterminated to fresh water for 90 min.

Study on Osteological Characteristics of Acanthorhodeus gracilis (가시납지리 (Acanthorhodeus gracilis)의 골격학적 연구(硏究))

  • Kim, Ik-Soo;Kim, In-Ja
    • Korean Journal of Ichthyology
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    • v.10 no.2
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    • pp.231-240
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    • 1998
  • The characteristics of cranium, vertebral column and fin supports of Acanthorhodeus gracilis were examined. The fish did not have a fontanell in cranium nor process of supraethmoid. It had double orbitosphenoids and a basioccipital process developed from anterior portion of basioccipital. It had a high and triangular dorsal process on supraoccipital. In the urohyal, hypohyal attachment was bifurcated and horizontal and vertical plate were in an elongated rhombus shape with posterior edge pointed. It had teeth of 1-rowed, 5/5 and 4 free teeth. The fish did not have a coracoid foramen on shoulder girdle. It does not have a uroneural on ural centrum. It had a long and slender posterior process of pelvic bone. It had a large and flat supraneural. The fish had 14 or 15 interneural spines, of which the 1st, 2nd, 3rd and 4th had single basiosts and the rest had double basiosts. It had 11 or 12 interhemal spines, of which the 1st, 2nd, 3rd and 4th had single basiosts and the rest had double basiosts.

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Effects of Preprocessing on Quality of Fermented Red Snow Crab Chionoecetes japonicus Sauce (전처리 방법에 따른 홍게(Chionoecetes japonicus) 어간장의 제조 및 품질변화)

  • Lim, Ji-Hoon;Jeong, Jee-Hee;Jeong, Min-Jung;Jeong, In-Hak;Kim, Byoung-Mok
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.48 no.3
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    • pp.284-292
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    • 2015
  • We explored preprocessing-mediated quality changes in red snow crab fish sauce. A control (C) group and groups treated with autolysis (A), boiling (B), enzymatic hydrolysis (E), and addition of Aspergillus oryzae (K) were formed. The titratable acidity of the K group increased with storage time, whereas that of groups C, A, B, and E decreased. The total and amino nitrogen contents initially increased on storage of all samples, but decreased in later periods. The total plate count (TPC) of the K group was initially 5.26 log CFU/mL and increased to 7.28 log CFU/mL at 3 months of storage. The TPCs of the C, A, B, and E groups were initially <5.00 log CFU/mL and decreased with storage. The lactic acid bacteria count of the K group was initially 4.80 log CFU/mL and increased until month 5 to approximately 6.06 log CFU/mL. The K group scored higher in terms of sensory attributes than the other groups and maintained marketable scores for all relevant properties (color, flavor, off-odor, and overall acceptance). Furthermore, the free amino acid content of the K group was the highest among all groups at approximately 3,000 mg per 100 g. These results suggest that K treatment may be beneficial in the preparation of fermented fish sauce.

BACTERIOLOGICAL STUDIES ON MARKET SEA FOODS 1. Sanitary Indicative Bacteria in Sundried Sea Foods. (시판 수산식품에 대한 세균학적 연구 1. 건제품의 위생지표 세균에 관하여)

  • CHANG Dong-Suck;CHOE Wi-Kyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.6 no.3_4
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    • pp.87-91
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    • 1973
  • This study was conducted to evaluate the sanitary quality of sun dried sea foods being distributed in the markets located in Busan city. Twenty one kinds of sun dried sea foods, 9 kinds of fish and shellfish, 5 kinds of mollusca and 7 kinds of seaweed were examined during the Period from September to December in 1970. For the evaluation of sanitary contents of sanitary indicative bacteria such as coliform group, fecal coliform, fecal streptococci and enterococci, and plate counts were determined. The results obtained were as follows: 1. Through out all the samples examined, the numbers of fecal streptococci and enterocccci were much greater than those of coliform group and fecal coliform. 2. Generally, the contents of enterococci exceeded MPN of 1,000 per 100 grams through all samples examined, while fecal coliform MPN'S were less than 18. 3. The median value of coliform group MPN was 78 and those of fecal streptococci MPN was 3,300 per 100 grams, The median value of the plate counts was $9.9\times10^4$ per gram, 4. Bacterial densities of the foods which had many chances to come into contact with hands were much higher than those of other foods which had not. 5. The bacterial contamination rate of seaweed was much lower than those of fish and shellfish. 6. No correlation between the numbers of sanitary indicative bacteria and plate counts was observed.

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Study On The Midwater Trawl Available in the Korean Waters - 1 . Attitude and Opening Opening Efficiency of Otter Board - (한국 근해에 있어서의 중층 트로올의 연구 - 1 . 전개판의 동작상태의 전개성능 -)

  • 이병기
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.22 no.4
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    • pp.41-48
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    • 1986
  • To investigate the midwater trawl gear available for the Korean near sea trawlers, the authors carried out a field experiment on the attitude and the opening efficiency of the otter board with the Pusan 404 (160GT, 750ps), a training ship of National Fisheries University of Pusan. The experimental trawl gear was designed to be operable by the ship and the otter board was made of single iron plate with 12% camber ratio. The special-prepared potentiometric angle detector was used for determining the attitude, and the 50 KHz fish finder for the opening between the otter boards. The results obtained can be summarized as follows: 1. The angle of attack varied by moving the towing point on the towing plate of the otter board. It showed 33 to 36 degrees when the point was set at the outmost position, 25 to 31 degrees at the middle position and 19 to 30 degrees at the inmost position, with a decreasing tendency according to the increase of towing speed in each cases. 2. The heel of the otter board always occured inwards, increasing slightly according to the increase of towing speed. 3. The tilt of the otter board always occured outwards, increasing slightly according to the move of the towing point inwards, and decreasing slightly according to the increase of towing speed. 4. The opening between otter boards showed the largest value when the towing point was set at the outmost position.

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