• Asian-Australasian Journal of Animal Sciences
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• 제12권4호
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• pp.554-557
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• 1999

Hatchery waste including infertile eggs, dead embryos in shell, dead or low grade chicks was cooked at $100^{\circ}C$ for 15 minutes and then oven dried and ground. Hatchery waste meal (HWM) thus prepared contained 44.25% protein, 4,573 kcal/kg gross energy, 3,600 kcal/kg metabolizable energy, 30% ether extract, 1.9% fibre, 14% ash, 9.8% nitrogen free extract, 7.26% Ca, 0.84%P, 1.86% lysine and 0.66% methionine with no Salmonella and E. coli. In biological evaluation trial, significantly higher weight gain was observed in ration containing 12% HWM compared with that containing similar amount of fish meal. Protein efficiency ratio on the two rations was 3.96 vs 2.85; protein digestibility, 86.02 vs 71.9; net protein utilization, 64.9 vs 42.37 and biological value, 75.37 vs 58.84, respectively, indicating better balance of amino acids in HWM compared with fish meal. Growth performance trial on broiler chicks also revealed better weight gain and feed efficiency on ration containing 12% HWM than that containing similar level of fish meal.

• Asian-Australasian Journal of Animal Sciences
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• 제10권6호
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• pp.629-634
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• 1997

An experiment was conducted to investigate the effects of equiprotein replacement of dietary fish meal (FM) with duckweed (DW) and soybean meal (SBM) on the performance of broilers. A total of 112 seven-day-old as hatched broilers were fed on 4 different iso-energetic (2,818 kcal/kg) and iso-nitrogeneous (20.2% CP) diets up to 56 days of age. Diet A was control with 12% FM. In diets B (3% DW + 13.5% SBM), C (6% DW + 11.5% SBM) and D (9% DW +10% SBM). All FM protein of control diet was replaced by DW and SBM. The replacement of dietary FM by DW and SBM depressed feed intake, live weight gain and feed conversion efficiency and increased production cost and thus affected profitability. All those growth parameters had a linear declining trend as the proportion of DW in the diet was increased. It may be concluded that complete replacement of dietary FM by DW and SBM should not be recommended for raising broilers.

• 한국수산과학회지
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• 제29권2호
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• pp.135-142
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• 1996

여러 가지 어분의 이용성을 검토하기 위해 어분의 종류와 혼합비를 달리한 실험사료로 2회에 걸쳐 사육 실험을 실시하였다. 단백질원으로 북양어분 간접식을 $100\%$ 첨가한 사료와 북양어분을 갈색어분으로 $100\%$ 대체한 2종류의 실험사료를 제조하여 평균체중 5.7g의 조피볼락을 대상으로 45일간 사육한 결과, 최종평균체중, 증중율 및 사료효율 모두 갈색어분 첨가구가 대조구보다 유의하게 낮은 값을 보인 반면 (P<0.05), 일간 사료섭취량, 단백질 및 지질축적율과 단백질 및 지질의 외견상 소화율은 대조구와 차이가 없었다. 단백질원으펀 북양어분 간접식 $100\%$ 첨가구 (대조구)와 북양어된 간접식을 북양어분 직접식으로 $50\%$ 및 $100\%$ 대체한 실험구, 갈색어분으로 $50\%$ 및 $100\%$ 대체한 실험구 및 어류가수분해단백질로 $50\%$ 대체한 실험구를 설정하여 평균체중 86.6g의 조피볼락을 21주간 사육한 결과, 사육기간에 따른 체중의 변화는 사료간에 거의 차이가 없이 직선적으로 증가하였으며, 최종평균체중, 증중율, 사료효율, 지질축적율은 어분종류에 따른 유의차가 인정되지 않았다(P>0.05). 단백질축적율은 갈색어분 $50\%$ 대체구가 유의적으로 높은 값을 보였으나(P<0.05), 모든 실험구의 값이 $22.4\~25.0\%$의 범위로 큰 차이는 없었다. 두 실험 모두 전어체의 단백질 및 지질 함량은 사료의 어분종류나 첨가비에 따른 차이는 없었다(P>0.05). 위의 결과들로 보아 성장효과가 어체 크기에 따라 달라짐을 알 수 있고, 성장함에 따라 어분 이용성의 선택 폭이 넓어지므로 어체 크기에 따라 배합사료의 주 단백질원을 적절히 조정하여 값비싼 북양 어분 간접식은 다른 값싼 어분으로 일부 또는 완전히 대체 가능할 것으로 판단된다.

• 한국수산과학회지
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• 제51권2호
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• pp.148-156
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• 2018

This study was conducted to evaluate dietary hydrolyzed pig bristle meal (PBM) for juvenile olive flounder Paralichthys olivaceus. In Experiment 1 (EXP-1), six experimental diets were prepared to contain 0, 3, 6, 9, 12 and 15% PBM (designated Con, PBM3, PBM6, PBM9, PBM12 and PBM15, respectively). Triplicate groups of olive flounder (initial body weight, 8.69 g) were fed the diets to apparent satiation for 8 weeks during the optimal water temperature season ($20.5{\pm}2.12^{\circ}C$). All PBM supplemented groups except for PBM3 showed significantly lower growth performance and feed utilization compared to the control group. The protein digestibility of PBM3, PBM6, and PBM9 diets did not significantly differ from that of the control diet. In Experiment 2, 1% mono-calcium phosphate was added into the experimental diets used in Exp-1. Triplicate groups of olive flounder (10.6 g) were fed the diets to apparent satiation for 8 weeks during the low water temperature season ($12.5{\pm}1.12^{\circ}C$). The growth performances and feed utilization of fish fed all diets except for PBM15 diet did not significantly differ from those of the control diet. This study indicates that hydrolyzed PBM can replace fish meal by up to 12% with limiting amino acids and mono-calcium phosphate in diets for juvenile olive flounder.

• 한국수산과학회지
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• 제54권3호
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• pp.350-357
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• 2021

This study aimed to evaluate how fish meal (FM) replacement in diets with a mixture of animal and plant protein sources affect growth performance, feed utilization, hematological parameters and innate immunity of red seabream Pagrus major. A control FM diet was formulated to contain 65% FM (Con). Two other diets were prepared replacing FM in the control diet with a mixture of protein sources (wheat gluten, soy-protein concentrate, tankage meal, and poultry by-product meal) by 30 and 40% (FM30 and FM40, respectively). Total 300 red seabream (body weight, 77.6±0.3g) were distributed to 12 tanks (300 L) in 4 replicates per diet. The fish were fed the diets to apparent satiation for 19 weeks. After the feeding trial, no significant differences could be observed in growth performance, feed utilization, hematological parameters, innate immunity, and survivals among all the dietary treatments. This long-term feeding trial at low water temperature (13.8-17.5℃) indicates that a proper mixture ratio of wheat gluten, soy protein concentrate, tankage meal, and poultry by-product meal can replace FM up to 40% in red seabream diets.

• Fisheries and Aquatic Sciences
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• 제25권11호
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• pp.537-548
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• 2022

Apparent digestibility coefficients (ADCs) of dry matter, crude protein, crude lipid, nitrogen-free extract, energy and essential amino acids in animal-based feed ingredients were determined for olive flounder (Paralichthys olivaceus). A reference diet (RF) was formulated to contain 1.0% chromic oxide (Cr₂O₃) as an inert indicator. Nine test diets were formulated to contain RF and one of the feed ingredients (pollock meal [PM], jack mackerel meal [JMM], anchovy meal [AM], cod meal [CM], sardine meal [SM], sand eel meal [SEM], tuna meal [TM], meat meal [MM] and squid liver meal [SLM]) at a 7:3 ratio in each diet designated as PM, JMM, AM, CM, SM, SEM, TM, MM and SLM, respectively. Olive flounder, averaging 150 ± 8.0 g, were stocked at a density of 25 fish per tank in 400-L fiberglass tanks attached with fecal collection columns. Feces were collected from triplicate groups of fish one time a day for four weeks. Dry matter and crude protein ADCs of CM and SEM were significantly higher than the other tested ingredients. Lipid ADCs of JMM, CM and SEM were significantly higher than the other test ingredients. Energy ADCs of CM and SEM were significantly higher than that of the other tested ingredients. The availability of amino acids in CM was generally higher than the other animal protein sources. PM exhibited the lowest amino acid availability among the treatments. Interestingly, MM exhibited significantly higher nutrient digestibility than several marine-based ingredients. However, CM and SEM are seeming to be highly digestible and effective to use in olive flounder diet compared to the other tested ingredients. Overall, the results of this study provide information about the bioavailability of nutrients and energy in animal feedstuffs to apply when formulating cost-effective practical feeds for olive flounder.

• 한국수산과학회지
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• 제42권3호
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• pp.243-249
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• 2009

This study was conducted to determine the proper inclusion levels of squid liver powder (SLP) and dehulled soybean meal (DHSM) as a fish meal (FM) replacement in the diets for olive flounders. All the experimental diets were formulated to be isonitrogenous (50% crude protein, CP) and isocaloric (17.1 kJ energy $g^{-1}$ diet). Four diet were formulation of FM with SLP (DHSM) were tested at 0, 25, 50, 75% (SLP(DHSM)$_0$, SLP(DHSM)$_{25}$, SLP(DHSM)$_{50}$ and SLP(DHSM)$_{75}$, respectively). Triplicate groups of 12 fish with an average body weight of 5.56$\pm$0.1 g (mean$\pm$S.D.) were fed one of three experimental diets for 7 weeks. Weight gain (WG) and the specific growth rate(SGR) of fish fed SLP(DHSM)$_0$ and SLP(DHSM)$_{25}$ diets were significantly higher than those of fish fed SLP(DHSM)$_{50}$ and SLP(DHSM)$_{75}$ diets (P<0.05). Also, WG and SGR of fish fed SLP(DHSM)$_{50}$ had significantly higher WG and SGR than that of fish fed SLP(DHSM)$_{75}$ (P<0.05). The feed efficiency (FE) and protein efficiency ratio (PER) of fish fed SLP(DHSM)$_0$ and SLP(DHSM)$_{25}$ were significantly higher than those fed SLP(DHSM)$_{50}$ and SLP(DHSM)$_{75}$ (P<0.05). The whole-body crude lipid content of fish SLP(DHSM)$_{75}$ was significantly higher than that of fish fed SLP(DHSM)$_{50}$ (P<0.05). However, there were no significant differences in whole-body moisture, crude protein and crude ash of fish fed all diets. Based on these growth performance results, the SLP(DHSM) combination replace up to 25% FM protein by the ANOVA test in juvenile olive flounder diets.

• 한국수산과학회지
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• 제32권2호
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• pp.149-154
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• 1999

이 연구는 16주 동안 치어기 조피볼락 사료에 있어서 어분단백질에 대한 어분대체품의 이용 가능성을 결정하고자 실시하였다. 어분대체품 (FMA)은 혈분 (BM), 오징어간분 (SLP), 육골분(MBM), 수지박 (LM), 가금부산물 (PBP) 및 우모분 (FM)등으로 제작$\cdot$사용하였고, 필수아민노산 (EAA) 중 2가지 (Methionine& Lysine)를 첨가하여 사용하였다. 5가지의 실험사료는 조단백질 $50\%$로, 가용성 에너지 15.6KJ/g으로 조정하였다. 실험사료는 조단백질을 기준으로 어분단백질과 어분대체품 비율을 다음과 같이 요약하였다 :사료 1, $100\%$ WFM: 사료 2, $87.5\%$ WFM : $12.5\%$ FMA, 사료 3, $75\%$ WFM : $25\%$ FMA;사료 4, $75\%$ WFM : $25\%$ FMA+2 EAAs; 사료 5, $50\%$ WFM : $50\%$ FMA+2 EAAs (Methionine& Lysine). 증체율, 사료효율, 일간성장률 및 단백질 전환효율에 있어서 사료 2 ($12.5\%$ FMA)는 사료 1 (대조구)에 비해 유의적인 차이가 없었지만 (P>0.05), 나머지 사료구들은 대조구에 비해 유의적으로 낮게 나타났다 (P>0.05). 필수아미노산을 첨가한 사료 3 ($25\%$ FMA)과 사료 4($25\%$ FMA+2EAAs)의 두 사료구간에서는 사료구 4가 유의적으로 높은 경향을 보였다(P<0.05). 따라서, 치어기 조피볼락에 있어서 필수아미노산 첨가없이 어분단 백질을 복합 어분대체품 (FMA)으로 $12.5\%$ 정도 대체 가능함을 알 수 있었고, 2개의 필수아미노산의 첨가는 어분대체품에 사용되었을 경우에는 어느 정도 효과가 나타났다.

• 한국식생활문화학회지
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• 제26권1호
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• pp.53-62
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• 2011

The middle school subjects were 65.8% girls and 34.2% boys. Seniors were most abundant at 56.3%. The most abundant location was Gyeonggi-do (54.7%), and 69.3% of subjects were living with 4?5 family members. Approximately 56% of the mothers had graduated from high school, and 58.1% were unemployed. The mother most often prepared the food (78.4%), fish was eaten mostly in the home, and taste was the most important factor (62.6%). The preferred home food menu was fish because it is healthy, and the main cause for disliking the school meal service was poor quality dishes compared to a home cooked meal. The acceptance and type of fish were proportional. Rice was the most favored dish to eat with fish and scored 3.97, the highest rank. Approximately 50% answered that fish was nutritious, and a significant difference was observed regarding the mother's educational background (p<0.05). Students who never had a lecture about fish answered that fish was not nutritious, which showed a significant difference with education (p<0.05). Approximately 44% of students ate fish 2?3 times per week, and male students showed a significantly higher intake of fish than female students (p<0.05). The poorly educated disliked fish the most, and the higher frequency intake of fish resulted in a significantly higher percentile (p<0.001).

• 수산해양교육연구
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• 제23권3호
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• pp.461-467
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• 2011

This experiment was conducted to determine the each level of a newly developed squid liver powder (SLP) as a dietary protein source replacing fish meal (FM) in juvenile flounder. A diet excluding SLP (FM diet) and a commercial diet were two control diets. Six experimental diets were formulated to replace FM with three types of SLP at 5 and 10% level, respectively (SLP-A5, SLP-A10, SLP-B5, SLP-B10, SLP-C5, SLP-C10). Prior to the start of a feeding trial, fish were fed the commercial diet for 1 week to adjust to the experimental conditions. Fish with average weight of $22.8{\pm}0.40g$ ($mean{\pm}SD$), were randomly distributed to each aquarium as groups of 20 fish and fed the experimental diets in triplicate at satiation for 8 weeks. After 8 weeks, weight gain and specific growth rate of fish fed SLP-C10 was lower than those of fish fed FM diet and SLP-B5. Meanwhile, no significant difference was observed in WG and SGR among fish fed diets excluding SLP-C10. Feed efficiency (FE) and protein efficiency ratio (PER) of fish fed each level of three types of SLP were not different from those of fish fed FM diet. However, fish fed SLP-C5 and SLP-C10 has lower FE and PER than those of fish fed a commercial diet, SLP-A5 and SLP-B5. Therefore, these results indicate clearly that dietary SLP-B could be a good protein source to develop inexpensive commercial diet in juvenile flounder.