• 한국수산과학회지
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• 제46권1호
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• pp.46-52
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• 2013

After an outbreak of viral disease in an aquafarm, release of virus (es) from infected fish into environmental seawater has been suspected. In the present study, we utilized a negatively charged membrane (HA type) as an efficient method for concentration and detection of fish pathogenic viruses, specifically, megalocytivirus and viral hemorrhagic septicemia virus (VHSV) present in field-collected seawater samples or inoculated into seawater artificially. Positively charged viruses adsorbed onto the negatively charged membrane and were eluted with 1 mM NaOH (pH 10.5) following rinsing with 0.5 mM $H_2SO_4$ (pH 3.0). Megalocytivirus and VHSV particles isolated using anegatively charged HA membrane from seawater inoculated with each virus at a concentration of 10 viral particles/mL were of sufficient quantity to show positive results in atwo-step PCR (or RT two-step PCR); however, despite it being negatively charged, a cellulose acetate (CA) membraneshowed negative results. In quantitative PCR, the detection limits of the HA membrane for megalocytivirus and VHSV in seawater were 1.20E+00 viral particles/mL and 1.22E+01 viralparticles/mL, respectively. The calculated mean recovery yields from 1 L seawater spiked with known concentrations of megalocytivirus and VHSV particles were 28.11% and 23.00%, respectively. The concentrate of a 1-L sample of culturing seawater from the aquatank of flounder suffering from VHSV showed clear positive results in PCR when isolated with an HA, but not a CA, membrane. Thus, viral isolation using an HA membrane is a practical and reliable method for detection of fish pathogenic viruses in seawater.

• Asian Pacific Journal of Cancer Prevention
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• 제13권1호
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• pp.329-337
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• 2012

Considerable attention has been given to the accuracy of HER-2 testing and the correlation between the results of different testing methods. This interest reflects the growing importance of HER-2 status in the management of patients with breast cancer. In this study the detection of HER-2 gene and centromere 17 status was evaluated using dual-colour primed in situ labelling (PRINS) in comparison with fluorescence in situ hybridization (FISH). These two methods were evaluated on a series of 27 formalin fixed paraffin embedded breast carcinoma tumours, previously tested for protein overexpression by HercepTest (grouped into Hercep 1+/0, 2+ and 3+). HER-2 gene amplification (ratio${\geq}2.2$) by PRINS was found in 3:3, 6:21 and 0:3 in IHC 3+, 2+ and 1+/0 cases, respectively. Comparing FISH and IHC (immunohistochemistry), showed the same results as for PRINS and IHC. Chromosome 17 aneusomy was found in 10 of 21 IHC 2+ cases (47.6%), of which 1 (10%) showed hypodisomy (chromosome 17 copy number per cell${\leq}1.75$), 7 (70%) showed low polysomy (chromosome 17 copy number per cell=2.26 - 3.75) and 2 (20%) showed high polysomy (chromosome 17 copy number per cell ${\geq}3.76$). The overall concordance of detection of HER-2 gene amplification by FISH and PRINS was 100% (27:27). Furthermore, both the level of HER-2 amplification and copy number of CEN17 analysis results correlated well between the two methods. In conclusion, PRINS is a reliable, reproducible technique and in our opinion can be used as an additional test to determine HER-2 status in breast tumours.

• 한국수산과학회지
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• 제38권6호
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• pp.372-378
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• 2005

An analysis method of tetracyclines far fish and shellfish products containing large amount of low molecular materials and pigments was established. The recovery of the established analysis method for four tetracycline samples was $72-100\%$ and higher than other methods reported. Especially, proposed sample treatment protocol was shown to be effective for the removal of low molecular materials and pigments that tend to interfere with accurate analysis. The detection limit of oxytetracycline (OTC) and tetracycline (TC) from the sample was 0.02 ppm, and the detection limit of chlortetracycline (CTC) and doxycycline (DC) from the sample was 0.1ppm, To examine the efficiency of the established method and identify tetracycline usage in fish farms, tetracycline group antibiotics in the flounder being cultured was monitored. The improved method can be used for fish and shellfish products effectively and all surveyed fish farms have used tetracycline all the year round. The proposed method was adopted as official method for fishery products by Korean Food and Drug Administration in 2003 and it is being used by regulatory authority as National Fishery Products Quality Inspection Service.

• 한국어병학회지
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• 제17권1호
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• pp.1-10
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• 2004

해수 환경 중의 VHSV 분포 조사를 위하여, 2003년 2월에서 5월까지 동해 및 남해안 인근 해역에 서식하는 자연산 해수 어류를 채집하였다. 바이러스 분리용 시료는 9종의 해수 어류로서 42개의 시료를 분석하였다. 각 시료의 조직여과액을 Epithelioma papulosum cyprini (EPC) cells에 접종한 결과, 고등어 Scomber japonicus와 숭어 Mugil cephalus에서 바이러스가 분리되었으며 이들 바이러스는 RT-PCR법을 이용하여 VHSV로 동정할 수 있었다. 고등어에서 VHSV가 검출되었다는 기보고는 있지만, 숭어에 대해서는 본 연구에서 처음으로 VHSV를 분리하였다. 자연산 어류에서 유래된 세 개의 VHSV isolates에 대한 glycoprotein gene을 분석한 결과, 이들 바이러스는 양식 넙치에서 질병을 유발하는 VHSV isolates와 유사하며 모두 genogroup I (American type)에 속하는 것으로 밝혀졌다.

• Parasites, Hosts and Diseases
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• 제58권6호
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• pp.647-652
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• 2020

Clonorchis sinensis is the most common fish-borne intestinal parasite in Korea. The aim of the present investigation was to survey the status of C. sinensis infection and analyze associated risk factors in residents of Haman-gun, Gyeongsangnam-do. A total of 5,114 residents from 10 administrative towns/villages voluntarily agreed to participate in the study, which comprised fecal examination, a questionnaire survey for risk factors, ultrasonography, and enzyme-linked immunosorbent assay for cancer biomarker detection in the blood. We detected C. sinensis eggs in 5.3% of the subjects. By region, Gunbuk-myeon had the highest number of residents with C. sinensis eggs. The infection rate and intensity were higher in male than in female residents. Based on the risk factor questionnaire, infection was highly associated with drinking, a history of C. sinensis infection, and the practice of eating of raw freshwater fish. Extension of the bile duct, infection intensity, and cancer biomarker detection significantly correlated with the presence of eggs in the study population. In conclusion, the development of feasible, long-term control policies and strategies for the elimination of C. sinensis in Korea is still required.

• 한국식품과학회지
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• 제35권6호
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• pp.1072-1078
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• 2003

방사선 조사된 건갑오징어로부터 유도된 hydrocarbon류와 2-alkylcyclobutanone류의 양을 비교 분석하였다. 건갑오징어의 지방추출은 Soxtec 기기를 이용하였으며, 지방추출 후 florisil 충진한 column을 이용하여 hydrocarbon류와 2-alkylcyclobutanone류를 분리하고 GC/MS 분석기기로 확인하였다. 0.5kGy부터 10kGy까지의 방사선 선량별로 조사된 각 시료에서 생성된 hydrocarbon류는 0.5kGy 이상 조사된 시료에서 검출되었으며 조사선량에 따라 생성량도 증가하였다. Palmitic acid와 stearic acid로부터 생성된 pentadecane과 1-tetradecene 그리고 heptadecane과 1-hexadecene의 함량이 가장 많았으며. 함량이 낮은 oleic acid로부터 생성된 8-heptadecene과 1,7-hexadecadiene은 가장 적은 함량으로 확인되었다. 2-Alkylcyclobutanone류는 0.5kGy 이상 조사된 시료에서 검출되었으며, palmitic acid로부터 생성된 2-dodecylcyclo-butanone이 두 시료 모두 가장 많이 생성되었다. Stearic acid로부터 생성된 2-tetradecylcyclobutanone 역시 조사선량과 지방산 함량에 따라 생성량이 증가되는 경향을 보였으나 그 함량이 너무 낮게 검출되었다. 본 연구 결과, 방사선 조사된 건오징어와 건갑오징로부터 생성된 hydrocarbon류는 조사선량에 따라 뚜렷하게 검출되었고 비조사 시료와 비교, 확인이 가능하여 방사선 조사 여부 판별뿐만 아니라 흡수선량을 예측하기 위한 marker로서 활용할 수 있으며, 2-alkylcyclobutanone류는 방사선 조사 유무의 marker로서 가능성을 확인하였다.

• 한국어병학회지
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• 제23권3호
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• pp.369-377
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• 2010

2005년부터 2007년 사이 매분기별로 포항, 울산, 기장, 거제, 완도 지역에서 채집한 양식넙치를 대상으로 기생충, 세균 및 바이러스에 대한 병원체 모니터링 결과를 분석하였다. 총 2,238마리 중 1,528마리(68.3%)에서 병원체가 검출되었다. 연도별 검출률을 비교한 결과, 2005년도에는 60.6%, 2006년도에는 66.7%, 2007년도에는 72.3%의 병원체 검출률을 나타내어 해마다 증가하는 것으로 나타났다. 조사시기별 총 병원체 검출률은 2월에 63.5%, 5월에 67.3%, 8월에 75.1%, 11월에 64.2%로 나타났다. 기생충, 세균 및 바이러스의 검출률은 각각 36.7%, 32.8%, 31.4%로 나타났다. 병원체의 단독감염률 및 혼합감염률은 각각 33.6%와 34.6%로 나타났다. 질병별 분포 조사에서 가장 높은 검출률을 나타낸 병원체는 Trichodina sp. (28.2%), viral nervous necrosis virus (24.3%), Vibrio spp. (11.6%), viral hemorrhagic septicaemia virus (10.5%) 순으로 나타났다.

• Mass Spectrometry Letters
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• 제8권1호
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• pp.8-13
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• 2017

Analysis of quinidine for fish tissues using single drop microextraction (SDME) coupled with atmospheric pressure matrix assisted laser desorption/ionization mass spectrometry (AP-MALDI-MS) are reported. Optimization conditions; such as extraction solvent, extraction time, pH of the aqueous solution, salt additions (NaCl), stirring rate, matrix type and concentration are investigated. Linear dynamic range (${\mu}M$), limit of detection, relative recovery%, and enrichment factor are 0.08-9.2, 0.05, $94.8{\pm}3.1-98.5{\pm}3.3%$, $4.34{\pm}0.28-4.40{\pm}0.30$, respectively. SDME-AP-MALDI-MS shows good intraday and interday reproducibility.

• Toxicological Research
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• 제24권1호
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• pp.23-28
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• 2008

Voltammetric analysis of mercury ions was developed using paste electrodes (PEs) with DNA and carbon nanotube mixed electrodes. The optimized analytical results of the cyclic voltammetry (CV) of the $1{\sim}14ng\;L^{-1}Hg(II)$ concentration and the square wave (SW) stripping voltammetry of the $1{\sim}12ng\;L^{-1}Hg(II)$ working range within an accumulation time of 400 seconds were obtained in 0.1 M $NH_4H_2PO_4$ electrolyte solutions of pH 4.0. For the relative standard deviations of the $1ng\;L^{-1}Hg(II)$, which were observed at 0.078% (n = 15) at the optimum conditions, the low detection limit (S/N) was pegged at $0.2ng\;L^{-1}(7.37{\times}10^{-13}M)$ for Hg(II). The results can be applied to assays in biological fish kidneys and wastewater samples.

• 한국어병학회지
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• 제33권1호
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• pp.1-6
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• 2020

In this study, carp edemavirus disease and herpesviral hematopoietic necrosis were examined in carp (Cyprinus carpio) and crucian carp (Carassius auratus) imported from China. From August 2016 to May 2017, 100 carp and 300 crucian carp were examined. There were no clinical symptoms in all the tested fish, and no internal or external parasitic infections were identified except for the detection of Argulus sp. in one leather carp. Carp edema virus disease was examined by PCR in C. carpio, but all were negative. In C. auratus, herpesviral hematopoietic necrosis was tested by PCR and 4 out of 300 were positive. The results of this study are expected to be useful as basic data for conducting risk analysis on the relevant organisms and counterpart countries or for designating new pathogens for quarantine.