• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.70-70
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• 2003

This study was conducted to determine the effects of the dietary microbial phytase (P) supplementation on growth performance and bioavailability of phosphorus in juvenile olive flounder, Paralichthys olivaceus and juvenile Korean rockfish, Sebastes schlegeli fed soybean meal-based diets. Nine experimental diets were formulated to be isonitrogenous and isocalolic to contain 50.0%crude protein (CP) and 16.7kJ, 48.6% crude protein (CP) and 16.0kJ available energy/g without the dietary phytase supplementation for olive flounder and Korean rockfish, respectively : 100% fish meal (FM); 70% FM ＋ 30% soybean meal (SM); 70% FM ＋ 30% SM ＋ Phytase(P) 1000U/kg diet; 70% FM ＋ 30% SM ＋ P 2000U/kg diet; 70% FM ＋ 30% SM with phytase-treated (Ptre) P 1000U/kg diet; 60% FM ＋ 40% SM; 60% FM ＋ 40% SM ＋ P 1000U/kg diet; 60% FM ＋ 40% SM ＋ P 2000U/kg diet; 60% PM ＋ 40% SM with Ptre P 1000U/kg diet. After two weeks of the conditioning period, triplicate groups of 25 fish initially averaging 6.15$\pm$0.04g, was randomly distributed into the aquarium for olive flounder, and 20 fish initially averaging 7.25$\pm$0.04g was randomly distributed into the aquarium for Korean rockfish. After 8 weeks feeding trials, there was no significant difference on weight gain fish fed between 100% FM and 70% FM＋30%SM with Ptre P 1000U in olive flounder and Korean rockfish. Apparent phosphorus digestibility fish fed phytase supplemental diets were significantly higher than those of fish fed phytase non-supplemental diets in olive flounder and Korean rockfish. Therefore, these results indicated that pre-treated soybean meal with phytase 1000U could replace fishmeal up to 30% for the maximum growth of juvenile olive flounder and Korean rockfish. Phytase supplementation could improve apparent digestibility of phosphorus in olive flounder and Korean rockfish fed soybean meal based diets.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.67-67
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• 2003

Dorsal aorta cannulated rainbow trout averaging 505 $\pm$ 6.5g (Mean $\pm$ SD) were divided into 7 groups (5 fish per group). Twenty four hour post feeding, cannulated fish were intubated with one of seven L-amino acid diets containing graded levels of Valine (Val. : 0.45, 0.95, 1.20, 1.45, 1.70, 1.95 or 2.45%) at 1% of body weight. Blood samples were taken at 0, 5 and 24hr after feeding the experimental diets. Post-prandial (5h after feeding) plasma-free Valine concentrations (PPval.) increased linearly from fish fed diets containing Valine between 0.45 and 1.45%, but the concentration remained constant from fish fed diets containing valine between 1.45 and 2.45%. Post-absorptive (24h after feeding) plasma free valine concentrations (PAval) increased linearly from fish fed diets containing valine between 0.45 and 1.45%, but the concentration remained constant from fish fed diets containing valine between 1.45 and 1.95%. Using the broken-line model analysis, the dietary valine requirement by PPval and PAval could be 1.44 and 1.50% in rainbow trout, respectively. These results supported that the estimated dietary valine requirement by PP(val.) and PA(val.) could be in close agreement with the values obtained from the previous conventional feeding method. Thus, the use of PFAA concentrations for determining essential amino acid requirements could be possible in rainbow trout with dorsal aorta cannulation.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2002.10a
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• pp.156-156
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• 2002

The goal of this study is to develop a biomarker used in monitoring abnormal behaviors of Japanese medaka (Oryzias latipes) as a model organism caused by hazardous chemicals that are toxic and persistent in the ecosystem. A widely used insecticide, diazinon (O, O-diethyl O- (2-isopropyl-4-methyl-6-pyrimidinyl) phosphorothioate), is highly neurotoxic to fish, and it is also well known that it causes vertebral malformation and behavioral changes of fish at relatively low concentrations. The fish behaviors were observed on a real time basis using an image processing and automatic data acquisition system. The genes potentially involved in the abnormal behaviors were cloned using suppression subtractive hybridization (SSH) technique. The untreated individuals showed common behavioral characteristics. When the test fish was affected by diazinon at a concentration of 0.1 and 1 ppm, some specific patterns were observed in its behavioral activity and locomotive tracks. The typical patterns were enhanced surfacing activity, opercular movement, erratic movement, tremors and convulsions as reported previously. The number of genes up-regulated tty diazinon treatment were 97 which includes 27 of unknown genes. The number of down-regulated genes were 99 including 60 of unknown genes. These gene expression patterns will be analyzed by the artificial neural networks such as self organization map (SOM) and multilayer perceptron (MLP), revealing the role of genes responsible for the behaviors. These results may provide molecular biological and neurobehavioral bases of a biomonitoring system for diazinon using a model organism such as fish.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2003.10a
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• pp.173-173
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• 2003

With the introduction of DNA microarrays, a high throughput analysis of gene expression is now possible as a replacement to the traditional time-consuming Southern-blot analysis. This cDNA microarray should be ahighly favored technology in the area of molecular toxicology or analysis of environmental stresses.In this study, therefore, we developed a novel cDNA microarray for analyzing stress-specific responses in japanese Medaka fish. In the design and fabrication of this stress specific functional cDNA microarray, 123 different genes in Medaka fish were selected from eighteen different stress responsive groups and spotted on a 25${\times}$75 mm glass surface. After exposure of the fish to bisphenol A which is the one of the well-known endocrine disrupting chemicals (EDCs), over 1 or 10 days, the responses of the DNA chip were found to show distinct expression patterns according to the mode of toxic actions from environmental toxicants. As a results, they showed specific gene expression pattern to bisphenol A, additionally, the chemical spesific biomarkers could be suggested based on the chip analysis data. Therefore, this chip can be used to monitor stress responses of unknown and/or known toxic chemicals using Medaka fish and may be used for the further development of biomarkers by utilizing the gene expression patterns for known contaminants.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.11a
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• pp.73-74
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• 2003

Three experiment were designed to determine the effects of dietary(n-3) fatty acids on the growth-suppressive effects of the inflammatory response and anti-oxidant enzyme activity and balances of nitrogen and energy. Day-old chicks were fed diets containing 1.0 ％ soybean oil in experiment 1, or diets containing 1.0 ％ of fish oil in experiment 2, and then on 7-d of age chicks were fed diets containing 1.0 ％ of either soybean oil or fish oil. In experiment 3, day-old chicks were fed on diets containing 1.0 ％ of either soybean oil or fish oil. In chicks of half pens within each dietary treatments of each experiments, the acute phase response were induced by injecting with Salmonella typhymurium lipopolysaccaride(LPS) i.p. 3 times at 8, 10 and 12-d of age. During acute phase response, chicks fed dietary fish oil from 1-d of age enhanced energy balances and did not give negative effect on nitrogen balance and uric acid excretion, but elevated MnSOD and Cu/ZnSOD activity In liver and erythrocyte cytosols compared with those fed dietary soybean oil. The results indicated that enhanced daily gain and metabolizable energy value of diet in birds fed fish oil from one-d of age seems due to change on the antioxidants systems during acute phase response.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2022.10a
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• pp.229-232
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• 2022

Recently, production of offshore fisheries in Korea has been decreasing. Since production of offshore fisheries in 2016 fell below 1 million tons for the first time in 44 years, it has not recovered and has been decreasing. In order to cope with such a decrease in fishery resources, the TAC (total allowable catch) system is implemented internationally for fisheries resource management. Since 1999, South Korea has introduced the TAC system to perform resource management. In this paper, we propose an artificial intelligence-based fish classification and weight estimation system that can be used to investigate fishery resources of land observers essential for the implementation of the TAC system. The system consists of an app and a cloud server that automatically measures the body size and height of fish and takes photos using a terminal equipped with a lidar sensor. In the cloud server, fish classification is performed using a CNN-based efficientnet model and the weight of fish is predicted using automatically measured body length and body height information. Using this system, it is possible to improve the existing method in which the land observer manually writes after measuring the tape measure and weight in the stomach market.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.78-79
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• 2003

Effects of dietary lipid level and source (squid liver oil being rich in n-3 HUFA, soybean oil being rich in 18:2n-6, and linseed oil being rich in 18:3n-3) in fishmeal-based diet on growth and body composition of grower sunshine bass raised in seawater were investigated. Fifteen grower (an initial weight of 146.8$\pm$0.23 g) sunshine bass were randomly distributed into 27 of 250 L fiber reinforced plastic flow-through tanks. Fish were hand-fed to satiety twice daily for 6 days a week throughout the feeding trial. Survival was over 97% and not significantly affected by either dietary lipid level or lipid source (n-3 highly unusaturated fatty acid, HUFA). Weight gain of fish tended to improve with dietary n-3 HUFA level up to 2.9%, but sharply decreased at 3.5%. The best weight gain was obtained in fish fed the diet supplemented with 6% squid liver oil and 3% soybean oil. FER and PER were not significantly affected by either dietary lipid level or dietary lipid source. The lowest moisture content of the whole body was observed in fish fed the diet supplemented with 12% squid liver oil and highest for the diet supplemented with 9% linseed oil, respectively. Protein content of fish was not significantly affected by either dietary lipid level or dietary lipid source. However, lipid content of the whole fish tended to increase with an increase of either dietary lipid level or dietary n-3 HUFA level, except for fish fed the diet supplemented with 9% linseed oil. Ash content of fish fed the diet with no supplementation of oil was highest and lowest for the diet supplemented with 9% soybean oil, respectively. Significant differences in saturated fatty acids (16:0, 18:0 and 24:0), monoene (18:1n-9), 18:2n-6, 20:5n-3 and sum of n-3 HFUA of fish were observed. In considering these results, it could be concluded that supplementation of 9% oil combined with 6% squid liver oil and 3% soybean oil into fishmeal-based diet was the most recommendable for growth of grower sunshine bass raised in seawater.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2003.09a
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• pp.98-101
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• 2003

To classify desired and undesired documents on the web according to each user's view, FOCUS (Fuzzy dOCUment ordering System) is developed based on fuzzy concept extraction, fuzzy fish eye matching, and fuzzy selection. Experiments are done using the concept-system-dictionary by EDR (Electronic Dictionary Research Institute) including 140,000 words and web-based documents related to movie.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2002.05a
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• pp.608-609
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• 2002

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2002.05a
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• pp.65-65
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• 2002