• Parasites, Hosts and Diseases
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• 제59권4호
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• pp.355-362
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• 2021

A rumen fluke Fischoederius elongatus is assigned to the type species of genus Fischoederius, family Gastrothylacidae. However, the mitochondrial sequences recently published are thought to be of inconsistent species, suggesting that several morphologically similar but genetically distinct species might be classified as Fischoederius elongatus. Thus, mentions of F. elongatus from South, Southeast, and East Asia might unintentionally refer to different species. The present work describes morphology and a full mitochondrial genome sequence of one of these species. The fluke specimens were collected from 2 infected cattle in Thailand. An interesting finding was the presence of a second tRNA-Asp gene next to a partial ND1 gene. It is suggested that these duplicated sequences are the remnants of non-reciprocal recombination events caused by inverted repeats located between ND2 and ND1 mitochondrial genes.

• Parasites, Hosts and Diseases
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• 제54권4호
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• pp.543-547
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• 2016

In the present study, we report on the occurrence of paramphistomes, Fischoederius cobboldi and Paramphistomum epiclitum, in Lao PDR with the basis of molecular data. Parasite materials were collected from bovines bred in Ban Lahanam area, Savannakhet Province, Lao PDR at Lahanam public market. Morphological observations indicated 2 different species of paramphistomes. The mitochondrial gene cox1 of the specimens was successfully amplified by PCR and DNA sequencing was carried out for diagnosis of 11 specimens. Pairwise alignment of cox1 sequences were performed and confirmed F. cobboldi and P. epiclitum infecting bovines in Laos. Although there were many limiting points, as the small number of worm samples, and the restricted access of the animal host materials, we confirmed for the first time that 2 species of paramphistomes, F. cobboldi and P. epiclitum, are distributed in Lao PDR. More studies are needed to confirm the paramphistome species present in Savannakhet and its hosts to clear the natural history of these parasites of ruminants in the region and measure the impact of this parasite infection in the life and health of the local people.

• Parasites, Hosts and Diseases
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• 제24권1호
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• pp.42-48
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• 1986

As a series of systematic classification of paramphistomes, in the first step, paramphistomes in the lumen and reticulum were collected on 170 Korean catties (2∼3 years age, male) slaughtered at Jeonju abattoir from July, 1984 to September, 1985 and were classified by means of morphology of the worms. Afterwards, the karyotype of Paramphistomum explanatum (Creplin, 1849) which is the common in Korean cattle was detected by means of modified air-drying method from testis cells of the worm. The following is a brief summary of the leading facts gained through the experiment. 1. Most of the cattle slaughtered at the abattoir were infected with paramphistomes. The 5 species of the worms were detected on 170 Korean cattle and the worm burden per head was from 2 to 784(on the average 170) worms, 120(70.59%) heads out of them involving 2∼100 worms. In 28,900 individuals of paramphistomes obtained on 170 Korean cattle, appearance rates of various worms were as follows : 49.74% in P. explanatum, 48.08% in P. cervi, 0.98% in Orthocoelium orthocoelium, 0.89% in Fischoederius cobboldi and 0.14% in Cotylophoron cotylcphorum. 2. The chromosome number of 620 P. explanatum in the haploid and diploid cells was n=9 and 2n=18, and abundant cells in meiotic division were observed; 1,420 haploid and 38 diploid cells were reliable. Nine pairs of mitotic chromosomes were homologous and the chromosomes were composed of aye medium-sized metacentrics(m), subtelocentrics(st) or submetacentrics(sm) and four smallsized subtelocentrics(st) or submetacentrics(am), while meiotic metaphase chromosomes were composed of five medium and four small-sized. 3. The haploid of the testis cells showed C-band in the centromeric region from 8 of them, whereas the remaining chromosome No. 5 included heterochromatin on the tip region, and chromosomes No. 3 and No. 7 showed a remarkable C-band distinguished from other chromosomes.

• Parasites, Hosts and Diseases
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• 제26권2호
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• pp.107-111
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• 1988

한국산 쌍구흡충의 계통분류를 하기 위한 연구치 일환으로 한우로부터 코볼드쌍구흡충(Fisthoederius robbozdi)을 채집한 다음 정소부위를 colchicine으로 단시간 처리하는 자연건조법을 응용하여 핵형을 분석하였다. 코볼드쌍구흡충 총 315개체의 생식세포에 대하여 염색체수를 조사한 바 n=9, 2n=18이었으며, 1,904개의 반수체성 중기염색체와 49개의 이배체성 중기염색체를 확인하였다. 이배체성 중기염색체는 7쌍의 중형염색체와 2쌍의 소형염색체로 구성되어 있었고, 반수체성 염색체는 중형 7개와 소형 2개로 구성되어 있었다. 동원체지수는 3번 염색체가 40.4%로서 9쌍중에서 동원체가 염색체의 가장 중앙부위에 위치하는 중부착사 염색체이었으며, 그 나머지의 것들은 32.4∼40.0%이었다. 분염법(C-banding)에 의한 생식세포의 반수체성 염색체는 핵형을 구성하는 이염색질이 거의 모든 염색체의 동원체 부위에 존재하였지만, 1번 염색체는 염색체 첨단에 이염색질이 농염되어 있었으며 .4, 6, 8번 염색체의 이염색질은 크고 진하게 관찰되었다.