• Journal of Life Science
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• v.33 no.1
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• pp.34-42
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• 2023

In a signal transduction network, from the perception of stress signals to stress-responsive gene ex- pression, binding of various transcription factors to cis-acting elements in stress-responsive promoters coordinate the adaptation of plants to abiotic stresses. Among the AP2/ERF transcription factor family genes, group VII ERF genes, such as RAP2.12, RAP2.2, RAP2.3, AtERF73/HRE1, and AtERF71/ HRE2, are known to be involved in the response to hypoxia stress in Arabidopsis. In this study, we dissected the HRE1 promoter to identify hypoxia-responsive region(s). The 1,000 bp upstream promoter region of HRE1 showed increased promoter activity in Arabidopsis protoplasts and transgenic plants under hypoxia conditions. Analysis of the promoter deletion series of HRE1, including 1,000 bp, 800 bp, 600 bp, 400 bp, 200 bp, 100 bp, and 50 bp upstream promoter regions, using firefly luciferase and GUS as reporter genes indicated that the -200 to -100 region of the HRE1 promoter is responsible for the transcriptional activation of HRE1 in response to hypoxia. In addition, we identified two putative hypoxia-responsive cis-acting elements, the ERF-binding site and DOF-binding site, in the -200 to -100 region of the HRE1 promoter, suggesting that the expression of HRE1 might be regulated via the ERF transcription factor(s) and/or DOF transcription factor(s). Collectively, our results suggest that HRE1 contains hypoxia-responsive cis-acting elements in the -200 to -100 region of its promoter.

• International Journal of Computer Science & Network Security
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• v.23 no.4
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• pp.55-68
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• 2023

In recent years, the Television White Space (TVWS) has attracted the interest of many researchers due to its propagation characteristics obtainable between 470MHz and 790MHz spectrum bands. The plenty of unused channels in the TV spectrum allows the secondary users (SUs) to use the channels for broadband services especially in rural areas. However, when the number of SUs increases in the TVWS wireless network the aggregate interference also increases. Aggregate interferences are the combined harmful interferences that can include both co-channel and adjacent interferences. The aggregate interference on the side of Primary Users (PUs) has been extensively scrutinized. Therefore, resource allocation (power and spectrum) is crucial when designing the TVWS network to avoid interferences from Secondary Users (SUs) to PUs and among SUs themselves. This paper proposes a model to improve the resource allocation for reducing the aggregate interface among SUs for broadband services in rural areas. The proposed model uses joint power and spectrum hybrid Firefly algorithm (FA), Genetic algorithm (GA), and Particle Swarm Optimization algorithm (PSO) which is considered the Co-channel interference (CCI) and Adjacent Channel Interference (ACI). The algorithm is integrated with the admission control algorithm so that; there is a possibility to remove some of the SUs in the TVWS network whenever the SINR threshold for SUs and PU are not met. We considered the infeasible system whereby all SUs and PU may not be supported simultaneously. Therefore, we proposed a joint spectrum and power allocation with an admission control algorithm whose better complexity and performance than the ones which have been proposed in the existing algorithms in the literature. The performance of the proposed algorithm is compared using the metrics such as sum throughput, PU SINR, algorithm running time and SU SINR less than threshold and the results show that the PSOFAGA with ELGR admission control algorithm has best performance compared to GA, PSO, FA, and FAGAPSO algorithms.

• Korean journal of applied entomology
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• v.42 no.3
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• pp.217-223
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• 2003

Effects of temperature on the development and reproduction of the Luciola lateralis were investigated at various temperatures. The development time of eggs, larvae, and pupae were shorter at higher temperatures than at lower ones. The insect did not develop at 10$^{\circ}C$ and 35$^{\circ}C$. The hatchability was 61.5% at 15$^{\circ}C$, 73.9% at 20$^{\circ}C$, 93.3% at 23$^{\circ}C$, 91.8% at 25$^{\circ}C$, 74.0% at 27$^{\circ}C$, and 46.0% at 30$^{\circ}C$, indicating the best hatchability rate at the temperature condition of 23 DC. Larval periods were 341.5:t 23.2 days at 15$^{\circ}C$, 265.5${\pm}$17.5 days at 20$^{\circ}C$, and 250.9${\pm}$11.7 days at 25$^{\circ}C$. Pupal periods were 94.7${\pm}$11.5 days at 15$^{\circ}C$, 41.7${\pm}$9.1 days at 20$^{\circ}C$, and 18.5${\pm}$7A days at 25$^{\circ}C$. Emergence rate was 23.3, 89.3 and 80.7%, respectively at the above temperatures. Adult longevity of female was 18.0 days at 15$^{\circ}C$, 2004 days at 20$^{\circ}C$, 10.7 days at 25$^{\circ}C$, and 5.8 days at 30$^{\circ}C$. Mean fecundity per female was higher at 20$^{\circ}C$ compared with at other temperatures. The developmental zero point temperatures (1) and the total effect temperatures (I<) of egg, larva, pupa, and complete development were 10.6, 14.0, and l3.1$^{\circ}C$ and 214.8, 1,564.8, and 229.2 degree-days, respectively. Mean generation time in days (T) was shorter at higher temperature. Net reproductive rate per generation (Ra) was the lowest at the highest temperature as well as at the lowest, and it was 177.19 which was the highest at 23$^{\circ}C$. The intrinsic rate of natural increase (r$\sub$m/) was highest at 27$^{\circ}C$ as 0.019. As a result, optimum range of temperature for L. lateralis growth was between 20-25$^{\circ}C$.

• Journal of Animal Science and Technology
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• v.54 no.5
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• pp.369-373
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• 2012

AMP-activated protein kinase (AMPK) maintains energy homeostasis in skeletal muscle. Nonetheless, its functional role on protein synthesis with different nutrient availability has not been elucidated. Therefore, the purpose of this study is to examine the effect of AMPK activity on protein content in C2C12 myotubes incubated with low (5 mM; LG) or high (25 mM; HG) glucose media. LG stimulated (p<0.05) AMPK and acetyl CoA carboxylase (ACC) activity compare to those in HG group. Total protein content was higher in myotubes cultured with HG than in those cultured with LG and further increased by AICAR (5-amino-1-${\beta}$-D-ribofuranosyl-imidazole-4-carboxamide). Myotubes cultured with HG showed 7.5% lower UbFL (Ubiquitin Firefly Luciferase)-to-SV40 (Simian virus40) ratio compared to those in LG. Glucose level did not change the phosphorylation level of mammalian target of rapamycin (mTOR). Interestingly, administration of AICAR significantly increased phosphorylation level of mTOR in myotubes cultured with LG but not in those with HG. Overall, this data indicate that AMPK activity and protein turnover are finely regulated in response to different glucose concentration.

• Korean journal of applied entomology
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• v.48 no.2
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• pp.203-209
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• 2009

This study was conducted to investigate the relationship between the climbing up event of Luciola lateralis larvae and water temperature. In the laboratory condition, the larvae did not come out of water at constant temperature condition of 19.3${\pm}$$0.3^{\circ}C$ and tried to climb up at varying temperature condition of 20${\sim}$$22^{\circ}C$ (mean temperature 20.9${\pm}$$0.9^{\circ}C$) without success. However, they climbed up at constant temperature condition of 20.8${\pm}$$0.6^{\circ}C$. The frequency of the larval climbing up was highest as 63.6% at 20${\sim}$$21^{\circ}C$. The most larvae climbed up at approximately $21^{\circ}C$ of average daily water temperature, In the natural condition, the larvae climbed up at 19.8${\sim}$$21.7^{\circ}C$ and the frequency of the larval climbing up was high as 80.4% at 20${\sim}$$21^{\circ}C$. The larval climbing up was also little observed at 19${\sim}$$20^{\circ}C$ at which no larval climbing up was found in the laboratory experiment. No larvae attempted to climb up when water temperature was below $19^{\circ}C$ and above $22^{\circ}C$. It took 18 days from climbing up of larvae to appearance of adults. In its habitat, the range of water temperature required for larvae to climb up is assumed to be 19.6${\sim}$$21.8^{\circ}C$ and the most suitable water temperature may be 20.4${\pm}$$2.3^{\circ}C$. Therefore, the range of water temperature required for larvae to climb up is assumed to be 19.8${\sim}$$21.7^{\circ}C$, and the suitable water temperature range for larvae may be 20${\sim}$$21^{\circ}C$, and the most suitable temperature is thought to be $21^{\circ}C$.

• Korean journal of applied entomology
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• v.39 no.4
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• pp.211-226
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• 2000

Genetic divergence and phylogenetic relationships among the major Korean fireflies (Hotaria papariensis, Luciola lateralis, and Pyrocoelia rufa) were studied. A portion of mitochondrial COI (403 bp) and 165 rRNA (490~504 bp) genes were sequenced, and the GenBank-registered, homologous 165 rRNA sequences of Japanese fireflies were compared (27 species of Lampyridae, one of Lycidae, and one of Rhgophthalmidae). Greatest DNA and/or amino acid sequence divergence was found when P rufa, belonging to Lampyrinae was compared with H. papariensis and L. lateralis, both belong-ing to Luciolinae, confirming the current taxonomic status of the species. In the PAUP and PHYLIP analyses with 165 rRNA data, grouping of the two geographic samples of H. papariensis with H. tsushimana validate the use of generic name, Hotaria. Nevertheless, lack of sister-group relationship of the two geographic samples of H. papariensis renders further investigation on this group . Although the Korean and Japanese L. lateralis formed a strong monophyletic group, a substantial genetic differentiation was detected between them (2.9% of 165 rRNA gene sequence divergence). Finally, the geographic samples of Korean p. rufa strongly formed a group with Japanese p. rufa, warranting the use of generic name, Pyrocoelia, but the genetic distance observed between the Cheju-Island individual and all others requires further investigation on this subject. Summarized, this study supports the current taxonomic status of the Korean fireflies in that each respectively formed a strong monophyletic group with its own species or genus.