• Asian-Australasian Journal of Animal Sciences
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• 제18권11호
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• pp.1557-1563
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• 2005

Ovarian follicular fluid contains both stimulatory and inhibitory agents that influence the growth and maturation of oocyte. In the present study, an attempt was made to isolate and study the biological properties of ovarian follicular fluid peptide(s) in buffaloes. Bubaline ovarian follicular was made steroid- and cell-free. A protein fraction was obtained by saturation (30-35% level) of the follicular fluid with ammonium sulfate. The protein fraction was purified with Sephadex-G 50 gel filtration chromatography and a single peak was obtained in the eluant volume, which was lyophilized. SDS-PAGE of the lyophilized fraction revealed a single band and the molecular weight of the peptide was 26.6 kDa. The peptide stimulated the cumulus cell expansion and in vitro maturation rate of oocytes in buffaloes in a dose dependent manner when it was incorporated at different dose levels (0, 10, 25, 50, 100 and 1,000 ng $ml^{-1}$ of maturation medium). The basic culture medium consisted of TCM 199 with Bovine serum albumin (0.3%). The in vitro maturation rates were comparable to those obtained with a positive control medium (TCM 199+20 ng EGF $ml^{-1}$+steer serum (20%)). Further purification and biological assays may throw more light on the nature and functions of this peptide.

• 한국지하수토양환경학회지:지하수토양환경
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• 제17권6호
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• pp.1-8
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• 2012

This study is to suggest a few efficient ways of rainwater utilization, through monitoring and analyzing 143 rainwater storage systems and 110 artificial recharge systems, which are installed in the recommended facilities by law, among the rainwater harvesting systems in Jeju Island. In the case that catchment facilities are damaged, rainwater could be contaminated by leaves and debris so that the rates of rainwater usages come to be lower. It is possible that contaminated rainwater could contaminate artificial recharge wells or rainwater discharging out of the rainwater harvesting system could result in flood and damage for the downgradient area. For maintaining high quality of rainwater and increasing rainwater utilization rate, it is necessary to install screening facilities and purification plant functioning precipitation and filtration. Also, in order to efficiently preclude the overflowing rainwater exceeding storage capacity, it is recommended to associate rainwater storage tanks with artificial recharge well or infiltration trench facilities.

• 혜화의학회지
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• 제21권1호
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• pp.163-174
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• 2012

Objective: The primary objective this study was to evaluate the effect of Paljeong-san (PJS) pharmacopuncture treatment on against the glycerol-induced acute renal failure in rats. Methods: Glycerol injection decreased GFR (glomerular filtration rate) and increased urine volume, serum creatinine, BUN level, urine albumine secretion and fractional excretion of Na+, K+. PJS was selected in the basis of invigorating kidney which can eliminate pathogens. Rats were treated with PJS pharmacopuncture on Shin-shu (BL23) and Chon-chu (ST25) point for 3 days, followed by 50% concentration of glycerol injection ($5m{\ell}/kg$ body weight). Results: After the 3 days treatment period, Paljeong-san (PJS) pharmacopuncture treatment improved renal function. In addition, Glycerol injection increased lipid peroxidation, and decreased Na-K-ATPase in renal cortex and which were prevented by PJS treatment. Conclusion: This study suggests that Paljeong-san (PJS) pharmacopuncture treatment show favorable effect on glycerol-induced acute renal failure in rats.

• 상하수도학회지
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• 제20권1호
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• pp.27-34
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• 2006

This study was conducted to evaluate the performance of a membrane bioreactor filled with high concentration of powdered activated carbon (HCPAC-MBR) to reduce DBPs at the drinking water treatment. The pilot system was installed after the rapid sand filtration process whose plant was the conventional treatment process. The removal efficiencies of DBPs were measured during pilot operation period of 2 years. HAA and THM removal rates could be maintained around 80~90% without any troubles and then tremendous reduction of HAA and THM reactivity were observed more than 52%. The average removal rate of HAA formation potential (FP) and THM formation potential (FP) were 70.5% and 67.6% respectively. It is clear that the PAC membrane bioreactor is highly applicable for advanced water treatment to control DBPs.

• 대한한방내과학회지
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• 제24권2_4호
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• pp.1046-1054
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• 2003

Renal Failure is called a disorder of kidney excretion induced by glomerulus filtration rate(GFR) decrease. GFR is measured by Blood Urea Nitrogen(BUN) and Cretinine in blood. This study is about Oriental diagnosis and treatment of Chronic renal failure(CRF) patients. We treated five cases which were diagnosed as CRF by using methods used in oriental medicine, the application of Youkmijihwang-tang(六味地黃湯加減). In most such cases, we concluded that the results turns better as the symptoms like fatigue and digestive disorder decreases and the decrease of BUN and Creatinine in blood as well.

• Childhood Kidney Diseases
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• 제22권1호
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• pp.22-27
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• 2018

Purpose: Podocytes are important architectures that maintain the crucial roles of glomerular filtration barrier functions. Despite this structural importance, however, the mechanisms of the changes in podocytes that can be an important pathogenesis of minimal change nephrotic syndrome (MCNS) are not clear yet. The aim of this study was to investigate whether apoptosis is induced by interleukin (IL)-13 in cultured human podocytes. Methods: Human podocytes were treated with different IL-13 doses and apoptotic cells were analyzed using terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL assay) and fluorescence-activated cell sorting (FACS). Results: The IL-13 increased the number of TUNEL-positive cells in a dose-dependent manner at 6 and 18 hours (P<0.05 and P<0.05, respectively). The apoptosis rate was appeared to be increased slightly in the IL-13-stimulated podocytes (8.63%, 13.02%, and 14.46%; 3, 10 and 30 ng/mL, respectively) than in the control cells (7.66%) at 12 hours by FACS assay. Conclusion: Our study revealed that IL-13 expression may increase podocyte apoptosis. Blocking the IL-13 signal pathway can potentially play an important role in regulating the apoptosis of podocytes.

• Journal of Pharmaceutical Investigation
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• 제24권3호spc1호
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• pp.11-18
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• 1994

We have studied the iron uptake by the purified brush-border membrane vesicles (BBMVs) to determine the effect of fructose on the absorption of iron. BBMVs were prepared by the modified calcium precipitation method, The degree of purification was routinely assessed by the marker enzyme, alkaline phosphatase, and the functional integrity was tested by $D-[1-^3H]glucose$ uptake. The appearance of membrane vesicles was shown by transmission electron microscopy (TEM). The uptakes of complexes of labeled iron $[^{59}Fe]$ with fructose and ascorbate were measured with a rapid filtration technique, The uptake rate and pattern of the two iron-complexes, Fe(III)-fructose and Fe(III)-ascorbate, were also observed. A typical overshooting uptake of D-glucose was observed with peak value of $2{\sim}3$ times higher concentration than that at equilibrium. This result was similar to other studies with BBMVs. TEM showed that the size of BBMVs was uniform and we can hardly find any contaminants, Fe(III)-fructose has the higher value of $V_{max}$ and the lower value of Km than those of Fe(III)-ascorbate, respectively. It may be concluded that D-fructose is more effective in promoting the iron absorption than ascorbate.

• Membrane and Water Treatment
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• 제7권5호
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• pp.377-401
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• 2016

To study the effect of titanium dioxide ($TiO_2$) nanoparticles on membrane performance and structure and to explore possible improvement of using mixed solvents in the casting solution, composite polyvinylidene fluoride (PVDF) ultrafiltration membranes were prepared via immersion precipitation method using a mixture of two solvents triethyl phosphate (TEP) and dimethylacetamide (DMAc) and addition of $TiO_2$ nanoparticles. Properties of the neat and composite membranes were characterized using scanning electron microscope (SEM), energy dispersive X-ray spectroscopy (EDS), Atomic force microscopy (AFM) and contact angle and membrane porosity measurements. The neat and composite membranes were further investigated in terms of BSA rejection and flux decline in cross flow filtration experiments. Following hydrophilicity improvement of the PVDF membrane by addition of 0.25 wt.% $TiO_2$, (from $70.53^{\circ}$ to $60.5^{\circ}$) degree of flux decline due to irreversible fouling resistance of the composite membrane reduced significantly and the flux recovery ratio (FRR) of 96.85% was obtained. The results showed that using mixed solvents (DMAc/TEP) with lower content of $TiO_2$ nanoparticles (0.25 wt.%) affected the sedimentation rate of nanoparticles and consequently the distribution of nanoparticles in the casting solution and membrane formation which influenced the properties of the ultimate composite membranes.

• Environmental Engineering Research
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• 제20권4호
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• pp.403-409
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• 2015

This study aims to investigate the use of a floating media filter (FMF) to eliminate waterborne microorganism in treated domestic wastewater for reuse in agriculture. A conventional sand filter (SF) was used concurrently to compare treated water quality. The total/fecal coliforms and somatic coliphage were employed as fecal indicators. The result showed that the FMF was fed with 3 times higher infiltration rate ($15m^3/m^2.h$) than that in the SF ($5m^3/m^2.h$), in which both filters gave similar coliform removal at 6 hours operation. The somatic coliphage elimination tended to increase with operational time for the FMF while that of the SF showed decreasing trend. When a 24 hour continuous operation was applied for the FMF, it showed better removal of somatic coliphage (78%), fecal coliforms (60%) and total coliforms (56%) than that of 6 hour operation. In conclusion, the FMF gave better performance than the SF did by producing a good quality of treated water for agriculture in terms of waterborne microorganisms including turbidity and suspended solids.

• Environmental Engineering Research
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• 제20권1호
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• pp.99-104
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• 2015

The purpose of this study is to evaluate the performance of forward osmosis (FO) system for harvesting microalgae cultivated in secondary sewage effluent. Microalgae species used in this study were chlorella sp. ADE4. The drawing agents used for forward osmosis system were seawater and concentrate of sea water reverse osmosis (SWRO) system. Chlorella sp. ADE4 cultured in secondary sewage effluent illustrated moderate efficiency in removal of total nitrogen (TN) (68%) and superior performance in total phosphorus (TP) removal (99%). Comparison of seawater and SWRO concentrate as drawing agent were made in FO membrane separation of the microalgae. The result from this study depicts that SWRO concentrate is strong drawing agent in FO membrane system providing an average dewatering rate of $4.8L/(m^2{\cdot}hr)$ compared to seawater with average dewatering of $2.9L/(m^2{\cdot}hr)$. Results obtained from this study indicated that FO system could be viable option for harvesting the microalgae for further biodiesel production. SWRO concentrate as a drawing agent could be very important finding in field of membrane technology for disposal of SWRO concentrate.