• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.84-84
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• 2002

The advantages of the OPS techniques(Vajta G et al, Mol Reprod Dev 51: 53-58,1998) give 1) high survival rates of various types of eggs, 2) quick and simple process, 3) inexpensive equipment and reduced chilling injury. The efficiency of IVM/IVF technique in the porcine species is relatively lower than that obtained in other species such as ruminants. Two experiments were designed to investigate the effects of in-vitro fertilization of porcine oocytes matures using different OPS protocol for chilling and warming of vitrification. Porcine oocytes from ovaries collected at abattoir were matured for 44 hours in TCM199 Earle's salt supplemental with pyruvate, pff, L-cysteine, hormones and gentamycin. Oocytes were denuded and fertilized with frozen boar semen by common method. Porcine embryos produced routinely by in-vitro culture system of NCSU23 medium. The vitrification and the warming were conducted by OPS method with the glass micropipette instead of straw vessels and modified the protocol of G.Vajta(1999). In Exp 1, Chilling/Warming:Holding Medium(HM)+EG+DMSO/HM +sucrose Medium(SM) at 39$^{\circ}C$ warm stage. In Exp 2, : PBS+CS+EG+Ficoll+ Trehalose/PBS+Trehalose at 25$^{\circ}C$ stage. Filling, freezing, packing, thawing out and further culturing were performed to follow the basic protocol of G Vajta. During IVM-lVC and post-warming, fertilization parameter and developmental potential were compared to and statistically analysed. It was not significantly different from Exp 1 and Exp 2 but 25$^{\circ}C$ of stage was slightly higher on the morula/blastocyst forming rate and better atmosphere for worker than that at 39$^{\circ}C$ stage.

• Journal of Technologic Dentistry
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• v.33 no.4
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• pp.505-516
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• 2011

Purpose: The purpose of this study is to supply basic data to establish new image for dental technicians by reflecting onto the education of dental technicians by grasping the image of dental technicians with the subjects of students in department of dental technology. Methods: It was studied with self-filling-in method through structured questionnaire from September 26, to October 21, 2011, with 421 subjects of the students in the department of dental technology in two colleges in Daegu. SPSS 18.0 for Windows was used to analyze the data, and frequency and percentage were used for statistic analysis, and correlation analysis and Multiple Linear Regression Analysis were used with mean and SD per each question. Results: It showed the highest recognition that the dental technicians is a 'specialist' as 4.66; the dental technicians has strong responsibility in his work as 4.42; the dental technicians takes an important role in improvement of public oral health as 4.39; and the dental technicians has professional intelligence and high technique as 4.33. So, it was found that they had image of professional and important role for oral health, and had their pride and selfconfidence as future dental technicians. Meanwhile, it showed that they had negative related-image as well as a job, that the dental technicians is too busy as 4.48; it requires improvement on image in the future as 4.27; it is a hard job with too much stress as 4.13. Conclusion: It is required for us and school to have continuous change and effort to make the students in the department of dental technology have positive mind for the dental technology to make them have pride for their job.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.4
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• pp.651-661
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• 2020

Objective: We hypothesized that Cr source can alter adipogenic-related transcriptional regulations and cell signaling. Therefore, the objective of the study was to evaluate the biological effects of chromium acetate (CrAc) on bovine intramuscular (IM) and subcutaneous (SC) adipose cells. Methods: Bovine preadipocytes isolated from two different adipose tissue depots; IM and SC were used to evaluate the effect of CrAc treatment during differentiation on adipogenic gene expression. Adipocytes were incubated with various doses of CrAc: 0 (differentiation media only, control), 0.1, 1, and 10 μM. Cells were harvested and then analyzed by real-time quantitative polymerase chain reaction in order to measure the quantity of adenosine monophosphate-activated protein kinase-α (AMPK-α), CCAAT enhancer binding protein-β (C/EBPβ), G protein-coupled receptor 41 (GPR41), GPR43, peroxisome proliferator-activated receptor-γ (PPARγ), and stearoyl CoA desaturase (SCD) mRNA relative to ribosomal protein subunit 9 (RPS9). The ratio of phosphorylated-AMPK (pAMPK) to AMPK was determined using a western blot technique in order to determine changing concentration. Results: The high dose (10 μM) of CrAc increased C/EBPβ, in both IM (p = 0.02) and SC (p = 0.02). Expression of PPARγ was upregulated by 10 μM of CrAc in IM but not in SC. Expression of SCD was also increased in both IM and SC with 10 μM of CrAc treatment. Addition of CrAc did not alter gene expression of glucose transporter 4, GPR41, or GPR43 in both IM and SC adipocytes. Addition of CrAc, resulted in a decreased pAMPKα to AMPKα ration (p<0.01) in IM. Conclusion: These data may indicate that Cr source may influence lipid filling in IM adipocytes via inhibitory action of AMPK phosphorylation and upregulating expression of adipogenic genes.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.38C no.5
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• pp.409-419
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• 2013

Kinect released by Microsoft in November 2010 is a motion sensing camera in xbox360 and gives depth and color images. However, Kinect camera also generates holes and noise around object boundaries in the obtained images because it uses infrared pattern. Also, boundary flickering phenomenon occurs. Therefore, we propose a real-time virtual-view video synthesis algorithm which results in a high-quality virtual view by solving these problems. In the proposed algorithm, holes around the boundary are filled by using the joint bilateral filter. Color image is converted into intensity image and then flickering pixels are searched by analyzing the variation of intensity and depth images. Finally, boundary flickering phenomenon can be reduced by converting values of flickering pixels into the maximum pixel value of a previous depth image and virtual views are generated by applying 3D warping technique. Holes existing on regions that are not part of occlusion region are also filled with a center pixel value of the highest reliability block after the final block reliability is calculated by using a block based gradient searching algorithm with block reliability. The experimental results show that the proposed algorithm generated the virtual view image in real-time.

• Smart Media Journal
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• v.1 no.3
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• pp.29-35
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• 2012

A depth image-based rendering (DIBR) technique is one of the rendering processes of virtual views with a color image and the corresponding depth map. The most important issue of DIBR is that the virtual view has no information at newly exposed areas, so called dis-occlusion. In this paper, we propose an intermediate view generation algorithm using the Kinect depth camera that utilizes the infrared structured light. After we capture a color image and its corresponding depth map, we pre-process the depth map. The pre-processed depth map is warped to the virtual viewpoint and filtered by median filtering to reduce the truncation error. Then, the color image is back-projected to the virtual viewpoint using the warped depth map. In order to fill out the remaining holes caused by dis-occlusion, we perform a background-based image in-painting operation. Finally, we obtain the synthesized image without any dis-occlusion. From experimental results, we have shown that the proposed algorithm generated very natural images in real-time.

• Restorative Dentistry and Endodontics
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• v.15 no.2
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• pp.1-16
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• 1990

This study was to evaluate the cytotoxic effect in vitro and the tissue response within the rat peritoneal cavity to high copper amalgam and glass ionomer-silver cement, suggested for use as a retrograde endodontic filling material. In the cytotoxicity experiment, the radioactively ($^{51}Cr$) labeled L929 mouse fibroblasts were employed to determine the relative cytotoxicity of two experimental materials. Those materials were evaluated immediately after set and after one and seven days setting. In the tissue response experiment, two experimental materials were to evaluate mean peritoneal cellular count, differential cell count and the content of silver and copper in pooled packed cells and eluate samples taken by peritoneal lavage technique, and compared with surgical control after one day. two, four and six weeks of implantation. The results were as following: 1. High copper amalgam exhibited significant cytotoxicity immediately after set but showed no sign of toxicity after one day and seven days setting materials. 2. Glass ionomer-silver cement showed no sign of toxicity immediately after set and after one day and seven days setting. 3. High copper amalgam and glass ionomer-silver cement groups produce no significant difference in the mean peritoneal cell count when compared with the surgical control group after one day, two and four weeks of implantation. Surgical control group exhibited significantly a greater cell count when compared with the High copper amalgam group after six weeks. 4. High copper amalgam group increased significantly in the percentage macrophages after four and six weeks of implantation when compared with surgical control group. 5. The trace metal analysis involved an increased silver content in the elutes and an increased copper content in the packed cells of high copper amalgam group, and an increased silver content in the packed cells and elutes of glass ionomer-silver cement group.

• Restorative Dentistry and Endodontics
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• v.29 no.4
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• pp.365-369
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• 2004

The purpose of this study was to evaluate the apical sealing ability of Super-EBA, MTA and Dyract-flow as retrofilling materials. Forty-eight extracted human teeth with straight and single root canal were used in this study. The root canals were prepared to a #40 apical canal size and obturated with gutter-percha. Apicoectomies were performed and root end cavities were prepared to a depth of 3mm using an ultrasonic device. The root end cavities were filled with Super-EBA, MTA or Dyract-flow. Leakage was measured using an electrochemical technique for 4 weeks. According to this study, the results were as follows. 1. Increasing leakage with time was observed in all groups. 2. No significant difference was noted among the 3 groups with time (p =0.216). 3. No significant difference was noted among the 3 groups when measured within the same time interval (p =0.814). The results of this study suggest that the sealing ability of Dyract-flow is equal to that of Super-EBA and MTA, and Dyract-flow may be an alternative to other materials for root-end filling.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.37 no.11
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• pp.1415-1421
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• 2013

Recently, to resolve problems regarding legal liability for accidents and disasters, various simulation techniques such as F.E.M. and F.V.M. have been used in the field of forensic engineering. In this study, we performed mechanical structure analysis using ADINA to investigate the cause of bridge collapse accidents. Such accidents occurred owing to modified and missing processes in comparison with the original design while filling with concrete. Modified and missing processes cause buckling of the upper plate and twisting of the main girder. Through this study, we determine the exact cause of bridge collapse by comparing the evaluation of the structure stability of the original design with the evaluation of the structure stability of the modified and missing process using ADINA structure analysis. Hence, this result indicates that buckling prediction through FEA is the most effective method.

• Restorative Dentistry and Endodontics
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• v.16 no.1
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• pp.121-132
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• 1991

The purpose of the study was to evaluate the marginal sealing effect of composite resin inlays according to the luting techniques and compare them to the conventional direct resin filling technique. 90 cavities of class V were prepared on the buccal surface of 90 extracted molar teeth, which were divided into four groups. Cavities of control group were directly filled with Scotchbond 2 and P - 50, and those of composite resin inlay groups were luted with one of the followings: Adhesive bond followed by Adhesive cement, All bond followed by Adhesive cement, Fuji - ionomer type L All the specimens were immersed in India ink dye solution for 7 days at $37^{\circ}C$ incubator after thermocycling between $5^{\circ}C$ and $60^{\circ}C$ and longitudinally sectioned with diamond disk inot two parts All the specimens were observed at the occlusal and gingival margins and statistical analysis was performed. The results were as follows: 1. Groups filled with composite resin inlay showed less marginal leakage than the group directly filled(p<0.01). 2. There was no significant difference in marginal leakage between composite resin inlay groups luted with Adhesive bond followed by Adhesive cement and the group luted with All bond followed by Adhesive cement(p>0.05). 3. At occlusal margins, Composite resin inlay group luted with Adhesive bond followed by Adhesive cement showed less marginal leakage than the group luted with Fuii ionomer type I(p<0.01). At gingival margins, composite resin inlay group luted with All bond followed by Adhesive cement showed less marignal leakage than the group luted with Fuji ionomer type I(P<0.01).

• Journal of The Korean Association of Information Education
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• v.4 no.1
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• pp.109-119
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• 2000

In this paper, we design and implement educational system by using active database technique in www environments. The developed system consists of three modules such as user group interface, active-based monitor, and expert group interface. The roles of each module are summarized as follows. The user group interface provides with system accessibility the users who registered in the developed system. The active-based monitor, triggering modules, consists of three sub-modules such as keyword parser, exception handler, and DB access module. Also it manages five items such as keyword index, Q&A DB, user group mailing list, and expert group mailing list. The expect group interface provides answer filling form for the expert who registered in the developed system.