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SPECTROSCOPIC AND CHEMOMETRIC ANALYSIS OF SW-NIR SPECTRA OF SUGARS AND FRUITS

  • Golic, Mirta;Walsh, Kerry;Lawson, Peter
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1133-1133
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    • 2001
  • Fruit sweetness, as indexed by total soluble solids (TSS), and fruit acidity are key factors in the description of the fruit eating quality. Our group has been using short wave NIR spectroscopy (SW-NIR; 700-1100 nm) in combination with chemometric methods (PLS and MLR) for the non-invasive determination of the fruit eating quality (1,2). In order to further improve calibration performance, we have investigated SW-NIR spectra of sucrose and D-glucose. In previous reports on the band assignment for these sugars in the 1100-2500 nm spectral region (3-7), it has been established that change in concentration, temperature and physical state of sugars reflects on the shape and position of the spectral bands in the whole NIR region(5-7). The effect of change in concentration and temperature of individual sugar solutions and sugar spiked Juice samples was analysed using combined spectroscopic (derivative, difference, 2D spectroscopy) and linear regression chemometric (PLS, MLR) techniques. The results have been compared with the spectral data of a range of fruit types, varying in TSS content and temperature. In the 800-950 nm spectral region, the B-coefficients for apples, peaches and nectarines resemble those generated in a calibration of pure sucrose in water (Fig. 1). As expected, these fruits exhibit better calibration and prediction results than those in which the B-coefficients were poorly related to those for sugar.(Figure omitted).

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A COMPARATIVE STUDY OF FREE AMINO ACIDS IN HEALTHY AND VIRUS DISEASED CHINESE DATE TREE (Virus에 감염된 대추나무의 병엽과 건전엽에 있어서의 유이 amino산의 정성적 비교)

  • Hong, Soon-Woo;Hah, Yung-Chil
    • Journal of Plant Biology
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    • v.4 no.1
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    • pp.9-11
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    • 1961
  • A comparative investigation of free amino acids content in healthy check and virus diseased leaves of Chinese date tree, Zyzyphus jujuqa Mill var. inermis Rhed, was carried out by authors throughout the growing season of 1959 and 1960 from June to October. The methods of qualitative analysis of free amino acids aplied in this experiment is followed by Moore and Stein. Free amino acids determined in this experiment are shown in Fig. 1 and Table 1. As the figure and the table are shown, three more amino acids such as glutamine, asparagine and histidine are detected in the diseased material. The additional amino acids which are known as diamines in diseased leaves are conspicuous. It is presumed that the diamine might be incresed by the self-reproduction of the virus in cooporation with certain enzymes which are carrying out the protein metabolism in the host protoplast in contrast with the healthy checks which is carrying out normal protein metabolism. From the histological poing of view, the facts of phloem degeneration or necrosis in diseased leaves, it seems to interrupt to move free amino acids from roots to leaves and it possibly takes place an excessive production of NH3 which is diaminated by the metabolism of nitrogen compounds in such conditioned leaves. Therefore, it is also presumed that additional diamino acids are accumulated in diseased leaves. There are no change of amino acids are accumulated in diseased leaves. There are no change of amino acids in both materials of this plant throughout the growing season qualitatively, and this result agress with the paper of Knight.

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Some Effects of Fire on Vegetation, Soil and Soil Microflora adjacent to DMZ in Korea (식생, 토양 및 토양미생물에 미치는 불의 효과에 대하여)

  • 홍순우
    • Journal of Plant Biology
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    • v.11 no.4
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    • pp.9-20
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    • 1968
  • In general there are few cases which were caused by lightening and no fire was observed to have been caused but by artificial or accidental fires during preliminary survey. And then the most scales of burning in Korea are like the fig. 2. Temperature 5 cm apart from flame at the burning site were known to range from 165$^{\circ}$to 20$0^{\circ}C$ in surface fire on Myozangdong, pine-quercus dominant community and from 120$^{\circ}$ to 145$^{\circ}C$ in surface fire of Wolwoonni, Mischanthus dominant area and from 120$^{\circ}$ to 14$0^{\circ}C$ in ground fire of Wangzinkun, Mischanthus dominant, respectively. Through the preliminary survey, fire indicater plants in Korea were confirmed as Mischanthus coreensis Hack and Carex alterifolia Franch. The plants of highest frequency appeared in the burnt sites were known to as Potentilla cryptotaeniae Max, Mischanthus coreensis Hack, Carex alterifolia Franch and Artemisia brachyphylla Kitamura. Lespedezas were proved as one of the fire tolerant and Pinus densiflora was found out as the lowest intolerant plant to fire. The acidity of burnt soil was decreased according to the considerable amount of ash left on the ground. The acidity of surface soil was more decreased than the below 10cm of soil. The chemical composition(NO3, P, K, Mg, Total-N and Organic-C) of burnt soil was more increased than those of the unburnt sites. And the population changes of soil microflora seemed to have a certain tendency. The population of soil microflora was increased a little according to climatic conditions. Also there was an initial decrease in the population of microflora followed immediately by a significant increase.

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The Historical Study of Korean Traditional Funtional Food (한국의 전통적 기능성 식품의 이용에 대한 역사적 고찰)

  • 한복진
    • Journal of the East Asian Society of Dietary Life
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    • v.6 no.2
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    • pp.235-255
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    • 1996
  • Natural substances, exploited in our daily life, have been applied to drugs to treat diseases and developed to functional foods by appropriate preparations, and these foods give beneficial effects on physical activities. In this paper, the utilization of traditional functional foods was studied with refer ring to old ancient writings published in the front-end of Chosun dynasty. The utilized vegetables were march mallow, turnip, radish, Chinese cabbage, lettuce, spinach, cucumber winter buds, flesh of a cabbage, eggplant, taro, burdock, Parsley, watershield plant, crown daisy, bamboo shoots, garlic, scallion, onion, acorn, bark of a tree, white goosefoot leaf, leaf of bean, pine mushroom, bracken. yam, mugwort, tea, ginseng, peppermint, fruit of the Maximowiczia chinensis, smartweed and pepper. The utilized fruits were chestnut, Chinese date, pine nuts, walnut, gingko nut, citrus. crab apple, pear, peach, grape, pomegranate, plum, Chinese quince, fig and watermelon. The utilized cereal were rice, barley, bean, buckweat and Job's-tears. The utilized sweetenings and seasonings were honey, wheat-gluten, sugar, oil, salt, soy sauce and vinegar. Our ancestors had a balanced diet using the various foods, and especially had a fundamental concept of "Foods have the efficacy of a remedy".edy".uot;.

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Study on the Optimal Design of Linear Control System Using Microcomputer - Design of PI Control System - (마이크로 컴퓨터를 이용한 모형 제어계의 최적설계에 관한 연구 - PI제어계 설계 -)

  • 양주호
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.25 no.1
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    • pp.29-34
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    • 1989
  • Recently microcomputers have come into wide use in the field of the control. They are used not only as monitors and as controllers in the various plant control systems but also as the Computer Aided Design of control systems. As an example of the design and the realtime control of the control system using Microcomputer, the optimal design of the control system of which the numerator of the transfer function is constant have been reported by authors. In this paper, author proposes a method to design the optimal controller about the control system of which the numerator of the transfer function is not constant, and develops the program of the response simulation.

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Developing Polyclonal Antibody-based Indirect-ELISA to Detect Anthracnose Inocula Prior to Soybean Sprout Rot

  • Park, Soo-Bong;Lim, Young-Ji;Lee, Jung-Han;Han, Ki-Soo;Lee, Sun-Cheol;Shim, Chang-Ki;Kang, Jin-Ho;Bae, Dong-Won;Kim, Dong-Kil;Kim, Hee-Kyu
    • The Plant Pathology Journal
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    • v.20 no.4
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    • pp.252-257
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    • 2004
  • We developed a polyclonal antibody based-ELISA system to monitor inocula accurately and rapidly before onset of anthracnose on soybean sprouts. Titer of mouse antisera against conidia of Colletotrichum gloeosporioides, determined by indirect ELISA, was high enough to be detectable up to ${\times}$25,600 dilutions. Both PAb1 and PAb2 had the highest level of reactivity to Colletotrichum gloeosporioides. Absorbance readings exceeded 0.15. Sensitivity of PAb to C. gloeosporioides was precise enough to detect spore concentration as low as 500 conidia/well by indirect ELISA. Both antibodies are very sensitive and highly specific to the target pathogen Colletotrichum gloeosporioides, apparently discriminating other unrelated pathogen, or epiphytes. This kit fulfills the requirements far detecting inocula before infection and onset of anthracnose. Our ELISA system should also be feasible to detect C. acutatum (Mungbean sprouts rot) and G. cingulata (C. gleosporioides), (apple, pepper). It was remarkable that absorbance value was not reduced even after 4 consecutive washings (Fig.4), suggesting that antigenic determinants are on the surface of conidia. Antigenic determinant was characterized by heating and enzyme treatment: Both PAb1 and PAb2 bind to protein epitope that does not contain residue of amino acid, arginine, and Iysine, even though more work needs to be done.

Fundamental Study on the Evapo- transaration Requirements of Patty rice Plant (벼 용수량계획상의 엽면증발량 및 주간수면 증발량에 관한 기초적인 연구)

  • 김철기
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.11 no.2
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    • pp.1651-1660
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    • 1969
  • The purpose of this study is to find out the reasonable amount of evapo-transpiration required for the paddy rice plant during the whole growing season. So. On the basis of the experimental data concerning the evapo-transpiration form 1966 to 1968, the author obtained the follow results. 1) The leaf area index in the densely planted plo is generally higher than that in the conventionally planted one during the first half of growing season So, the coefficient of transpiration in the former plot is somewaht higher than in the latter, and the coefficient of water surface evaporation under the plant cover has the inverse relation between both plots. 2) It is unreasonable that coefficient of evapo-transpiration is applied to the calculation of the evapo-transpiration requirements of each growing stage, because a degree of variation in meteorological factors and in the thickness of the plant growth is involved in it. 3) It is most reasonable that the rate of transpiration and of the water surface evapoation is applied to the calculation of the transpirated amount and evaporated one in each growing stage, because it shows almost constant value in spite of any meteorological conditions in so far as the variety of rice, planted density and control of applying fertilizer are sanme and the disease and blight are negligible. 4) The ratio of the amount of transpiration to the weight of the whole air dried yields has the tendency of decreasing as that of the yields increases having almost constant value despite the amount of pan evaporation; and the value is about 210 when the weight of root parts is included to that the yields. 5) Although the required amount of transpiration during the whole growing season can be calculated with the above ratio, Fig. 7 showing the relation between the amount of transpiration and the weight of the yields is more reasonable and will be convinient to find it. And the requirements of water surface evaporation during the same season can also be directly found witht theweight air dried straw refering to Fig. 8.

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Influence of Crown Gall Infection on Growth and Flowering of Rose (뿌리혹병 감염이 장미의 생육과 개화에 미치는 영향)

  • Han, Kyung-Sook;Kim, Won-Hee;Park, Jong-Han;Han, You-Kyoung;Cheong, Seung-Rong
    • Research in Plant Disease
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    • v.14 no.3
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    • pp.159-164
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    • 2008
  • Crown gall of rose (Rosa hybrida) was observed in greenhouse during 2003-2007. The average disease incidence was up to 38.1 % and was more severe in hydroponic culture as compared to soil culture. The typical gall symptom occurred mainly on the root, crown, or both, and resulted on poor rooting, growth retardation and yield loss. The reduction rate of rooting influenced by crown gall was 57.5% as compared to healthy plants on nursery stock. The location of gall formation in the plant influenced growth vigor resulting in symptoms such as poor shooting. Healthy plants produced 19.1 flowers/$m^2$, while diseased plants produced 9.5 flowers/$m^2$ during the same cultivation period. Moreover, the number of days to flowering was longer for the diseased plants than for healthy plants - 51.2 days and 39.8 days for first harvest, and 60.6 days and 52.1 days for the second harvest, respectively. Conclusively, infection on the basal stem caused serious loss of the number of shoot formation; yield loss of cut flower was 38.7% due to crown gall infection and delay of harvesting time about 8-10 days.

The Analysis of Flow-Induced Vibration and Design Improvement in KSNP Steam Generators of UCN #5, 6

  • Kim, Sang-Nyung;Cho, Yeon-Sik
    • Journal of Mechanical Science and Technology
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    • v.18 no.1
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    • pp.74-81
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    • 2004
  • The KSNP Steam Generators (Youngkwang Unit 3 and 4, Ulchin Unit 3 and 4) have a problem of U-tube fretting wear due to Flow Induced Vibration (FIV). In particular, the wear is localized and concentrated in a small area of upper part of U-bend in the Central Cavity region. The region has some conditions susceptible to the FIV, which are high flow velocity, high void fraction, and long unsupported span. Even though the FIV could be occurred by many mechanisms, the main mechanism would be fluid-elastic instability, or turbulent excitation. To remedy the problem, Eggcrate Flow Distribution Plate (EFDP) was installed in the Central Cavity region or Ulchin Unit 5 and 6 steam generators, so that it reduces the flow velocity in the region to a certain level. However, the cause of the FIV and the effectiveness of the EFDP was not thoroughly studied and checked. In this study, therefore the Stability Ratio (SR), which is the ratio of the actual velocity to the critical velocity, was compared between the value before the installation of EFDP and that after. Also the possibility of fluid-elastic instability of KSNP steam generator and the effectiveness of EFDP were checked based on the ATHOS3 code calculation and the Pettigrew's experimental results. The calculated results were plotted in a fluid-elastic instability criteria-diagram (Pettigrew, 1998, Fig. 9). The plotted result showed that KSNP steam generator with EFDP had the margin of Fluid-Elastic Instability by almost 25%.

Similarity Relationship between Basic Species of the Oak by the Numerical Method (수치분석(數値分析)에 의(依)한 참나무 기본종(基本種)의 유연관계(類緣關係))

  • Ma, Sang-Kyu
    • Journal of Korean Society of Forest Science
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    • v.21 no.1
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    • pp.47-51
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    • 1974
  • In order to prove the similarity relationships between the basic species of oak through Electronic Data Processing System(EDPS) and numerical analysis, The analized species and datas were selected from the list of morphological observation in the thesis of T.B. Lee, 1961, "Phytogenetic study of the subgenus Lepidobalanus of the genus Quercus in Korea", and were coded by categories shown in Table 1. The value in the list were transformed into hundred percentage to standardize the observational value by each code into dimensionless. The similarity index between species were computed through formula of non-metric coefficient, $N_{jk}=\sum\limits_{i=1}^{n}\(\frac{{\mid}x_{ij}-x_{ik}{\mid}}{x_{ij}+x_{ik}}\)$, using the UNiVAC-1106, at National Computer Center. Quercus aliena, by analysis result, is most similar to Q. mongolica with the similarity index, 71.6 and Q. dentata is most far apart from Q. serrata in the relationship with index, 121.4. The above thesis of Professor, T. Lee, are closely similar with the result of this research study. But, their similar relationship are proved in quantity through numerical method in our research study. In addition, The relationships among Q. mongolica, Q. aliena and Q. serrata are found to be very similar, but Q. dentata to be enough far in similarity to other species by dendrogram shown at Fig. 1. The numerical classification through EDPS is found to be suitable method also applicable to the plant taxonomy.

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