• Title/Summary/Keyword: Fiber confocal method

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Surface profiling by the phase shifting method in fiber-optical confocal scanning interference microscopes (광섬유 공초점 간섭 현미경과 위상 변위법을 결합한 표면 검색)

  • 김대찬;이승걸
    • Korean Journal of Optics and Photonics
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    • v.10 no.3
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    • pp.201-207
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    • 1999
  • The fiber-optical confocal scanning interference microscope with a simple configuration was constructed with a 4-port fiber-optic coupler, and the new method based on the phase shifting method was proposed for surface profiling by the system. In the method, the height of a specimen was determined from the phase of confocal beam. It was verified experimentally that the method was applicable to even the confocal interference microscope with a long-wavelength source and a low NA objective, and that the scanning time could be drastically reduced compared with the conventional method. Finally, it was found that our method is less sensitive to the variation of surface reflectivity than the conventional method.

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Analysis of External Fibrillation of Fiber by Confocal Reflection Microscopy (공초점반사현미경법을 이용한 섬유의 외부소섬유화 분석)

  • Kwon, Ohkyung
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.46 no.2
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    • pp.35-45
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    • 2014
  • Confocal Reflection Microscopy (CRM) was applied to investigate external fibrillation of different types of fibers such as Kajaani reference fiber, Whatman filter fiber, thermomechanical pulp (TMP), and recycled TMP fiber. It was confirmed that the CRM images are created from surface structures of the fiber cell wall. Confocal Laser Scanning Microscopy (CLSM) captured overall shape of the fiber, but minute details of the surface of the fiber were missed. CRM captured the minute details of the fiber surface. From the CRM and CLSM images, it was observed that the CRM images mainly appeared on the fiber surfaces. External fibrillation of the fiber occurs at the fiber surface, not inside the cell wall. Thus, it was concluded that investigation on the external fibrillation of the fiber was possible by utilizing CRM images. A direct qualtitative and quantitative method for analysis of external fibrillation of fiber was demonstrated by utilizing surface area to volume ratio, volume fraction, and roughness calculated from 3-dimensional images reconstructed from stacks of CRM images from the different fibers.

Fiber Orientation in Injection-Molded Short Fiber Composites with a Confocal Laser Scanning Microscope and Numerical Simulation (공초점 레이저 주사 현미경을 이용한 단섬유 복합재료 사출 성형물 내의 섬유 배열 측정 및 수치모사)

  • Lee, Kwang-Seok;Le, Seok-Won;Youn, Jae-Ryoun
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2001.05a
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    • pp.201-204
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    • 2001
  • A Confocal Laser Scanning Microscope (CLSM) is applied to determine three-dimensional fiber orientation states in injection-molded short fiber composites. Since the CLSM optically sections the composites, more than two planes either on or below the surface of composites can be obtained. Therefore, three dimensional fiber orientation states are determined without destruction. To predict the orientation states, velocity and temperature fields are calculated by using a hybrid FEM/FDM method. The change of orientation state during packing stage is also considered by employing a compressible Hele-Shaw model. The predicted orientation states show good agreement with measured ones. However, some differences are found at the end of cavity. They may result from other effects, which are not considered in the numerical analysis.

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Investigation of Layered Structure of Fiber Cell Wall in Korean Red Pine by Confocal Reflection Microscopy

  • Kwon, Ohkyung
    • Applied Microscopy
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    • v.44 no.2
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    • pp.61-67
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    • 2014
  • Layered structures of fiber cell wall of Korean red pine (Pinus densiflora) were investigated by confocal reflection microscopy (CRM). CRM micrographs revealed detailed structures of the fiber cell wall such as S1, S2, and S3 layers as well as transition layers (S12 and S23 layers), which are present between the S1, S2, and S3 layers. Microfibril angle (MFA) measurement was possible for the S2 and S3 layer in the cell wall. The experimental results suggest that CRM is a versatile microscopic method for investigation of layered structures and MFA measurement in individual sub layer of the tracheid cell wall.

Study on Auto Focusing System of Laser Beam by Using Fiber Confocal Method (파이버 공초점법을 이용한 레이저 빔 자동 초점 제어 장치에 관한 연구)

  • Moon, Seong-Wook;Kim, Jong-Bae;King, Sun-Hum;Bae, Han-Seong;Nam, Gi-Jung
    • Laser Solutions
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    • v.9 no.3
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    • pp.7-13
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    • 2006
  • Auto focusing system to find optimized focal position of laser beam used for material process has been investigated by using fiber confocal method. Wavelength of laser diode (LD) and diameter of single-mode fiber are 780nm and $5.3{\mu}m$, respectively. Intensity distributions of beam reflected from the surface of mirror and silicon bare wafer have been observed in a gaussian form. Experimental results show that focal position obtained by LD is shifted from one observed from surface scribed by laser about $80{\mu}m$. It is due to the difference of wavelength and each divergence of between LD and laser used for material process. It is confirmed that auto focusing control system through position calibration has operated steadily.

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Study on auto focusing system of laser beam by using fiber confocal method (파이버 공초점법을 이용한 레이저 빔 자동 초점 제어 장치에 관한 연구)

  • Moon, Seong-Wook;King, Sun-Hum;Kim, Jong-Bae;Bae, Han-Seong;Nam, Gi-Jung
    • Proceedings of the Korean Society of Laser Processing Conference
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    • 2006.11a
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    • pp.41-45
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    • 2006
  • Auto focusing system to find optimized focal position of laser beam used for material process has been investigated by using fiber confocal method. Wavelength of laser diode (LD) and diameter of single-mode fiber we 780nm and $5.3{\mu}m$, respectively. Intensity distributions of beam reflected from the surface of mirror and silicon bare wafer have been observed in a gaussian form. Experimental results show that focal position obtained by LD is shifted from one observed from surface scribed by laser about $80{\mu}m$. It is due to the difference of wavelength and each divergence of between LD and laser used for material process. It is confirmed that auto focusing control system through position calibration has operated steadily.

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Quantification of Fibers through Automatic Fiber Reconstruction from 3D Fluorescence Confocal Images

  • Park, Doyoung
    • Journal of Advanced Information Technology and Convergence
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    • v.10 no.1
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    • pp.25-36
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    • 2020
  • Motivation: Fibers as the extracellular filamentous structures determine the shape of the cytoskeletal structures. Their characterization and reconstruction from a 3D cellular image represent very useful quantitative information at the cellular level. In this paper, we presented a novel automatic method to extract fiber diameter distribution through a pipeline to reconstruct fibers from 3D fluorescence confocal images. The pipeline is composed of four steps: segmentation, skeletonization, template fitting and fiber tracking. Segmentation of fiber is achieved by defining an energy based on tensor voting framework. After skeletonizing segmented fibers, we fit a template for each seed point. Then, the fiber tracking step reconstructs fibers by finding the best match of the next fiber segment from the previous template. Thus, we define a fiber as a set of templates, based on which we calculate a diameter distribution of fibers.

Measurement of the Internal Structure of an Optical Waveguide Embedded in a Flexible Optical Circuit Board by Enhancing the Signal Contrast of a Confocal Microscope

  • Lee, Won-Jun;Kim, Dae-Chan;O, Beom-Hoan;Park, Se-Geun;Lee, El-Hang;Lee, Seung-Gol
    • Journal of the Optical Society of Korea
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    • v.15 no.1
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    • pp.9-14
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    • 2011
  • In this study, the internal structure of an optical waveguide embedded in a flexible optical circuit board is observed with a confocal microscope. In order to increase the light reflection from an internal material interface with a very small index difference, and thus enhance the signal contrast, a theta microscopy scheme has been integrated into a conventional confocal microscope, and a high NA oil-immersion lens has been used. The interface reflectivity is increased from roughly 0.0015% to 0.025% by the proposed method, and the internal structure can thus be successfully measured.

Measurements of Defects after Machining CFRP Holes Using High Speed Line Scan (고속 라인 스캔 방식을 이용한 CFRP 가공 홀 표면 및 내부 결함 검사)

  • Kim, Teaggyum;Kyung, Daesu;Son, Unchul;Park, Sun-Young
    • Journal of the Korean Society for Precision Engineering
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    • v.33 no.6
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    • pp.459-467
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    • 2016
  • Using a line scan camera and a Galvano mirror, we constructed a high-speed line-scanning microscope that can generate 2D images ($8000{\times}8000pixels$) without any moving parts. The line scanner consists of a Galvano mirror and a cylindrical lens, which creates a line focus that sweeps over the sample. The measured resolutions in the x (perpendicular to line focus) and y (parallel to line focus) directions are both $2{\mu}m$, with a 2X scan lens and a 3X relay lens. This optical system is useful for measuring defects, such as spalling, chipping, delamination, etc., on the surface of carbon fiber reinforced plastic (CFRP) holes after machining in conjunction with adjustments in the angle of LED lighting. Defects on the inner wall of holes are measured by line confocal laser scanning. This confocal method will be useful for analyzing defects after CFRP machining and for fast 3D image reconstruction.