• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.2
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• pp.45-48
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• 2001

In an effort to optimize tissue culture responses of tall fescue, the effects of basic medium and carbon sources on seed culturability and genotypic difference of plant regenerability were investigated. The MS basal medium was superior to $N_{5}$ 6/ and $B_{medium}$ in enhancing callus growth and plant regeneration. To determine the effect of carbon sources on plant regeneration, the seeds were cultured on medium with 30 g/L sucrose and maltose, respectively. Medium supplemented with 30 g/L sucrose resulted in regeneration of shoots from 50% of the calli. The genotypic difference in plant regenerability was obvious among five cultivars of tall fescue tested. \\`KY31\\` and \\`Hokuryo\\` showed to have higher regenerability with the frequency of 33% and 51%, respectively.

• Proceedings of the Korean Society of Grassland Science Conference
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• 2001.06a
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• pp.84.2-85
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• 2001

• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.2
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• pp.133-142
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• 1998

This study was conducted to investigate the effects of antagonistic bacteria and pathogenic fungi on the growth of tall fescue(Festuca arundinacea Schreb.) in continuous cropping soil(CCS) and non-continuous cropping soil(NCCS). Tall fescue was established by seeding into pots of 11 cm in diameter and 9 cm in depth containing 1 : 1 mixture of soil and vermiculite, and cultivated at pots with antagonistic bacteria and pathogenic fungi in a vinyl house. The bacteria used in this study were Bacillus subtilis and hsants. B. subtilis was isolated and identified kern forage rhizosphere soil and fusants were isolated through cell hsion from B. subtilis and B. thwingiensis. B. subtilis was named as B. subtilis 101 and hsants were named as F-3, F-7 and F-8. In dark culture experiment, tall fescue inoculated with the antagonistic bacteria lived longer than that of control in both CCS and NCCS. However, tall fescue of CCS lived shorter than that of NCCS. Dry weight of tall fescue inoculated with the antagonistic bacteria was higher than that of tall fescue inoculated with pathogenic hngi in both CCS and NCCS(P< 0.05), and the antagonistic bacteria showed positive effects on the growth of tall fescue. However, Dry weight of tall fescue was decreased by the inoculation of the pathogenic b g i in both CCS and NCCS(P< 0.05).

• Proceedings of the Korean Society of Grassland Science Conference
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• 2002.09a
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• pp.26-27
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• 2002

• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.3
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• pp.195-204
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• 1998

This study was designed to investigate the effects of antagonistic microorganism, Bacillus subtilis, on the growth of forage seedlings in repeated cultivation soils and unrepeated cultivation soils. The field experiment was wnducted in pots in a vinyl house using repeated and unrepeated cultivation soils. Forage types were 'Suwon 19' wrn(Zea mqs L.), 'Califbmia' white clover(Tr~oIium repens L.) and 'Fawn' tall fescue (Festuca arundianacea Schreb.). Samples of white clover and tall fescue were taken h m each pot at 36 days after seeding. Samples of wm were examined at 50 days after seeding. The most active antagonistic bacterium was isolated h m forage rhizosphere soil, and selected by reference to it's antagonistic ability on the growth of pathogenic fungi, Rhizoctonia solmi and Fusarium oxyspomm, and it was identified as Bacillus subtilis. This strain strongly suppressed the growth of fungal pathogens among isolated rhizobacteria. The dry weight of forage shoots and roots cultivated in unrepeated cultivation soils was higher than that cultivated in repeated cultivation soils. The dry weight of forage was positively affected by the inoculation of the antagonistic bacterium, Bacillus subtilis, in both repeated cultivation soils and unrepeated cultivation soils. In conclusion, the growth of forage was more affected by the inoculation of the antagonistic bacterium in unrepeated cultivation soils than that in repeated cultivation soils, and bacterization of forage with B. subtilis resulted in an inrreased yield.

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.1
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• pp.9-16
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• 1996

'This experiment was canied out to investigate the effects of endophyte status(end0phyte-free and -infect) and cutting frequency(3, 4, 5 and 6 timeslyear) on the agronomic characteristics, DM yield and weed development of tall fescue(Festuca arundinacea Schreb.) at the Experimental Field of Grassland and Forage Crops Division, National Livestock Research Institute, Suweon, from 1993 to 1994. 'Ihe results obtained are summarized as follows: DM yield of endophyte-infected tall fescue was slightly higher than that of endophyte-free tall fescue. DM yield of two tall fescue varieties was significantly decreased with frequent culting(p<0.05). Weed development of pasture tended to be decreased with endophyte-infected tall fescue and infrequent cutting, and endophyte-infected tall fescue had a good adaptation to the poor environment. Based on the results of this experiment, it is suggested that a slight increase in forage yield could be obtained from endophyte-infected tall fescue and endophyte-infected tall fescue was more persistant than endophyte-free tall fescue under poor conditions.

• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.4
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• pp.363-370
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• 1997

This experiment was carried out to investigate the effect of the mixed rates of endophyte-he and -infected seed on the endophyte fkquency, dry matter yield and forage quality of tall fescue(Festuca arundinacea Schreb.) at the Experimental Field of Grassland and Forage Crops Division, National Livestock Research Institute, Suweon, from 1993 to 1994. The results obtained are summarized as follows: Endophyte frequency was increased with mixed rates of higher endophyte-infected seed, however, it was not different between mixed rates of endophyte-free and -infected seed. DM yield of tall fescue was not different between mixed rates of endophyte-fke and -infected seed, and CP, NDF, ADF and IVDMD contents of forage quality were not influenced by mixed rates of endophyte-he and -infected seed. However, the weed contents were slightly increased with high level of endophyte-free seed. The results demonstrated that endophyk-fke tall fescue did not seem to be greatly weak under poor conditions, if the weed contents were slightly increase.

• Journal of The Korean Society of Grassland and Forage Science
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• v.5 no.2
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• pp.106-110
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• 1985

This trial was carried out to find out the optimum ridge and seeding rate for the seed production of Festuca arundinacea S. (variety Alta). It was composed of three ridge level (12, 18 and 24cm) and four seeding rates (10, 15, 20 and 25kg/ha) in a split-plot design with 4 replications. The experiment lasted from September 1979 to October 1981. 1. The beginning of heading state was May 12 and the date of full flowering stage was June 2. The optimum stage for the seed harvesting of Festuca arundinacea was about 43 days after beginning of heading stage. 2. The number of panicles were 157 per square meter from the best treatment 18cm ridge with 15kg seed/ha. And the 1000 grain weight was 2.79g for the treatment 12cm ridge with 10kg seed/ha. 3. The average seed yield for two years was 681kg/ha and the largest 831kg/ha for the treatment 18cm ridge with 25kg seed/ha. 4. the average germination rate of the harvested seed was 90.7 percent, and it showed a little higher germination rate from the lower level of seeding rate. 5. The average total DM production with two cuttings of aftermath was about 6715kg/ha. According to the increasing seeding rate and narrowing the ridge, DM yield was higher.

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.1
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• pp.17-26
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• 1996

This experiment was carried out to investigate the effects of endophyte staWendophyte-free and -infect) and cutting frequency(3, 4, 5 and 6 timeslyear) on the forage quality of tall fescue(Festuca crnrtzdit~crcerr Schreb.) at the Experimental Field of Grassland and Forage Crops Division. National Livestock Research Institute, Suweon, from 1993 to 1994. 'Ihe results obtained are summarized as follows: CP content of tall fexue was increased with endophyte-free and cutting frequency for two varieties. Crude protein yield(CPY) of tall fescue in 1993 was increased with endophyte-free and cutting frequency for two varieties(P<0.05). In 1994, however. CPY of endophyte-free tall fescue was slightly lower than that of endophyte-infected tall fescue and the higher CPY was observed when tall fescue was harvested 6 times per year. NDF content of tall fescue was not different between endophyte-infected and -free. NDF was decreased with cutting frequency for two varieties in 1993, however. it was not influenced by the endophyte and cutting frequency in 1994. ADF content of tall fescue was not different between endophyte-infected and -free. and it was decreased with cutting frequency for two varieties. IVDMD was not influenced by endophyte, however, it was increased with cutting frequency for two varieties. Based on the results of this experiment, it is suggested that a slight increase in forage quality could be obtained from endophyte-free tall fescue and the quality was increased with frequent cutting.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.5
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• pp.577-582
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• 1996

In order to investigate the relationship between daily herbage allowance (DHA) and daily herbage intake (DHI) on the points of leaf and stem masses, grazing experiments were carried out on the pasture of tall fescue (Festuca arundinacea Schreb.). The experiments consisted of four 3-days' grazing periods which were from August 14 to 17, August 17 to 20, October 12 to 15 and October 15 to 18 in 1990. The value of DHA was 32.1 to 84.3, and the value of DHI was 9.9 to 27.0. The index of leafiness (leaf to stem ratio, LSR) was 0.74 to 3.06. The relationship between DHI and DHA was quadratic. Both relationship of daily leaf intake (DLI) to daily leaf allowance (DLA) and that of daily stem intake (DSI) to daily stem allowance (DSA) were likely to be asymptotic forms, although DHI did not show any significant relationships to DLA nor to DSA. The relationship between DLI and LSR was quadratic, and a negative relationship was found between DSI and LSR. From the relationship of DLI to LSR and that of DSI to LSR, the relationship of daily intake of leaf and stem (DLSI) to LSR was derived as quadratic and the LSR value at which the maximum DLSI was attained was estimated. The DHA, DHI, DLA, DLI, DSA, DSI and DLAI were expressed as the same unit of g/kg live body weight/day.