• Biotechnology and Bioprocess Engineering:BBE
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• 제6권1호
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• pp.1-5
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• 2001

The potential use of spin filter device to retain Propionibacterium acidipropionici in the bioreactor under continuous mode of fermentation, and improve acid productivity, was examined. The yield of propionic acid based on lactose concentration was 51% in batch and 54% in continuous (dilution rate = 0.05 h(sup)-1) operation. The yield in continuous fermentation with cell retention using spin filter of 10 micron size (dilution rate = 0.05 h(sup)-1) was even higher at 70% (w/w). The volumetric productivity under batch and continuous mode of operation were 0.312g L(sup)-1 h(sup)-1 and 0.718g L(sup)-1 h(sup)-1 respectively. Continuous fermentation with cell retention demonstrated even higher volumetric productivities at 0.98g L(sup)-1 h(sup)-1 with out clogging problems. It could be used for utilization of cheese whey to produce propionic acid at higher yield and productivities.

• 한국식품영양학회지
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• 제30권5호
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• pp.1007-1014
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• 2017

To compare functional Chinese cabbage('Amtak' baechu; F1 hybrid cultivar between Brassica rapa and B. perkinensis, AB) with general Chinese cabbage ('Chunkwang' baechu; general spring cultivar, CB), two kinds of kimchi(ABK and CBK) prepared with AB and CB cultivar were fermented at $10^{\circ}C$ for 10 days. Their fermentative characteristics and anti-proliferative activities against mouse carcinoma cell lines were investigated. General kimchi(CBK) showed mature pH on the $6^{th}$ day of fermentation, whereas functional kimchi(ABK) reached pH on the $9^{th}$ day. CBK also exhibited acidity of mature stage on the $6^{th}$ day, but ABK reached mature acidity on the $9^{th}$ day. Although ABK and CBK were salted in the same condition, ABK had lower salinity than CBK, throughout the fermentation time. The highest total bacterial and lactic bacterial counts of CBK showed on the $8^{th}$ day of fermentation, but ABK showed the highest total bacterial and lactic bacterial counts on the $10^{th}$ day. The texture of ABK was harder than CBK for fermentation time. This seems to be corrleated with the slower fermentation rate of ABK. ABK showed significantly higher anti-proliferative activity (54.6% cell viability of control) in B16BL6 at $1,000{\mu}g/mL$. ABK was also higher in anti-proliferative activity than CBK throughout the fermentation time. However, there was no significant difference in the anti-proliferative activity of ABK between the fermentation times. In conclusion, fermentation of ABK showed a better texture, due to the slow fermentation rate and more anti-proliferative activity against mouse carcinoma cell line than those of CBK.

• 한국식품영양과학회지
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• 제26권5호
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• pp.794-799
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• 1997

The effective fermentation methods of Kimchi juice for utilization of outer layer of Chinese cabbage, an waste of Kimchi industry were studied. The Kimchi juice prepared with brining and grinding the waste of Chinese cabbage and addition of spices was fermented at $25^{\circ}C$. Addition of 5$^{\circ}C$15% fermented Kimchi juice of pH 5.4 at initial stage and pH 4.4 at middle state resulted in a significant increase in fermentation rate and solid content after 12 hours of fermentation. The combined method of enzymatic hydrolysis(0.1% viscozyme) of the brined and ground cabbage and addition of 2.0% NaCl, 1.0% sucrose and 10% fermented juice of pH 5.4 first and 4.4 during fermentation, respectively resulted in more rapid fermentation. The solid concentration was 5 times higher than control at maximum point and acidic and total flavor intensity were also significantly high.

• 한국미생물·생명공학회지
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• 제10권3호
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• pp.223-226
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• 1982

통기발효에서 중요한 통기 및 진탕효과를 측정함에 있어 공기로부터 물속으로 용존해 가는 산소의 전달속도와, 용존된 산소가 미생물에 의해 흡수되는 속도를 산출하였다. 항생물질발효에서 통기효과는 현저하였으며, 배양시간에 따른 산소흡수 속도와 비산소흡수 속도가 변화하는 것을 알았다. 이러한 변화는 대사이차 산물인 항생물질생합성의 생화학적인 전환에 기인하는 것으로 판단된다.

• 한국식품과학회지
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• 제25권4호
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• pp.391-394
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• 1993

탁주발효의 자동화를 위하여 발효 중 발생하는 기체를 측정할 수 있는 bubble counter와 제어장치를 개발하였다. 동 장치를 활용하여 pilot 규모의 탁주발효에 적용하였다. 탁주발효중 발생하는 기체를 계측하는 bubble counter는 photo-interruptor와 acryl관으로 구성하였으며, 계측되는 기체용적은 기포당 0.018ml였다. 그리고 탁주발효의 제어장치는 single chip microcomputer(MC68705R3)로 제작하였으며, 발효액의 온도를 측정, 제어하기 위하여 온도계측회로를 제작하였고, 냉각수 순환을 결정하는 solenoid valve의 작동으로 온도제어를 하였다. 동 장치를 운영하기 위하여 software를 작성하여 ROM에 구조화하였다. 동 장치로 $CO_2$ 발생량과 속도를 자동적으로 추정한 결과 알코올 농도곡선과 형태가 서로 유사함을 보여주었으며, $30^{\circ}C$ 발효조건에서 24시간때에 최대 발생량을 보였다. 알코올농도와 $CO_2$ 발생량은 뛰어난 상관관계를 보였으며, 이로부터 제어알고리듬을 작성할 수 있었다.

• 한국식품영양학회지
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• 제14권5호
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• pp.385-390
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• 2001

1. 비가열 시료인 a와 b가 PE의 활성은 저장 둘째 주에 급격히 증가하다가 감소하였고, 가열 시료인 c 는 저장 넷째 주에 증가하다가 감소하였고, 병행 열처리와 trehalose 처리 시료는 저장 끝까지도 증가하였다. 2. PG의 활성은 비가열 시료인 a와 b는 저장 둘째 주부터 감소하다 저장 끝에는 증가하였고, 가열 시료인 c와 d는 저장 기간 동안 변화가 거의 없었다. 3. POD의 활성은 비가열 시료인 a와 b는 저장 둘째 주에 증가하다 감소하였고, 가열 시료인 c는 둘째 주에 약간씩 감소하였고, 병용 열처리하고 trehalose 처리군은 감소하였다. 4. 관능검사 결과 시료 a. b, d는 아작함과 기호도가 전 저장 기간동안 높았고, 시료 c는 갈색과 신맛의 증가로 기호도가 낮았다 저장기간에 따라서는 2주차에는 아작함과 형태가 좋았으나 4주 6주로 가면서 아작함과 기호도의 값이 감소하였다. 그리고 각 시료별 기호도에 가장 영향을 주는 항목은 아작함이었고 시료 c는 갈색, 맛, 조직감 등에서 음의 관계를 나타냈다.

• Journal of Microbiology and Biotechnology
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• 제22권3호
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• pp.400-406
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• 2012

To improve the hydrogen yield from biological fermentation of organic wastewater, a co-culture system of dark- and photo-fermentation bacteria was investigated. In a pure-culture system of the dark-fermentation bacterium Clostridium butyricum, a pH of 6.25 was found to be optimal, resulting in a hydrogen production rate of 18.7 ml-$H_2/l/h$. On the other hand, the photosynthetic bacterium Rhodobacter sphaeroides could produce the most hydrogen at 1.81mol-$H_2/mol$-glucose at pH 7.0. The maximum specific growth rate of R. sphaeroides was determined to be 2.93 $h^{-1}$ when acetic acid was used as the carbon source, a result that was significantly higher than that obtained using either glucose or a mixture of volatile fatty acids (VFAs). Acetic acid best supported R. sphaeroides cell growth but not hydrogen production. In the co-culture system with glucose, hydrogen could be steadily produced without any lag phase. There were distinguishable inflection points in a plot of accumulated hydrogen over time, resulting from the dynamic production or consumption of VFAs by the interaction between the dark- and photo-fermentation bacteria. Lastly, the hydrogen production rate of a repeated fed-batch run was 15.9 ml-$H_2/l/h$, which was achievable in a sustainable manner.

• 환경위생공학
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• 제16권2호
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• pp.32-37
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• 2001

The purpose of this study was to investigate the created liquid post-acid fermentation of usability of denitrification as exterior carbon sources by pH control. The time of acid fermentation of food waste, the slower loading capacity of organic matter, the faster decomposition rate, but the density of generation Volatile Fermentation Acids(VFAs), was weak and, $SBOD_{5}$ : ST-N rate and $SBOD_{5}/SCOD_{Cr}$ rate was low. Between TS and VS, VS was decreased to 4.5th day fast, and then was decreased slowly. 1.5 days after stating the experiments, $SCOD_{Cr}$, $SBOD_{5}$, STOC and $VFA_{s}$ was decreased of increased slowly, and then increased fast. And after showing the highest density, it was tended to decreased fast. At the time of $SBOD_{5}$ with the highest density, at $SBOD_{5}$ : ST-N ratio, $R_1$ was 303:1, $R_2$ was 319:1, $R_3$was the highest. After studying $SBOD_{5}$ : ST-N ratio and $SBOD_{5}/SCOD_{Cr}$ ratio, as a carbon source of biological denitrification it was profitable composition ratio.

• KSBB Journal
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• 제7권4호
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• pp.258-264
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• 1992

효모를 이용한 에탄올 발효에서 세포 농도가 130g/L일 때 배양액은 약간의 가소성 유체의 성질을 보이며 세포 농도가 증가할수록 cell floc의 형성으로 인하여 세포 분리를 위한 막의 투과 성능은 향상되었다. 세포재순환 에탄올 발효에서 최적 세포농도를 발효액의 유변학적 성질, 막의 투과 성능, 그리고 에탄올 생산성을 고려하여 결정하였다. 또한 최적의 세포 농도에서 최대 에탄올 생산성과 최대 포도당 전환율을 위한 희석 속도를 최적화하였다. 최적의 세포 농도는 130g/L이었고 이 농도에서 최적의 희석 속도는 $1.3h^{-1}$이었다.

• Journal of Microbiology and Biotechnology
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• 제16권12호
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• pp.1849-1855
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• 2006

It has been shown that the initial conditions of bacterial cultivation are extremely important for the successful production of hyaluronic acid (HA) by fermentation. We investigated several parameters that affect cell growth rate and the productivity and molecular weight of hyaluronic acid--i.e., agitation speed, aeration rate, culture temperature, pH, and pressure--to determine how to optimize the production of HA by Streptococcus zooepidemicus on an industrial scale. Using a 30-1 jar fermentor under laboratory conditions, we achieved maximum HA productivity and biomass when the agitation speed and aeration rate were increased simultaneously. By shifting the temperature downward from 35$^{\circ}C$ to 32$^{\circ}C$ at key levels of cell growth during the fermentation process, we were able to obtain HA with a molecular weight of $2.8{\times}10^6$ at a productivity of 5.3 g/l. Moreover, we reproduced these optimized conditions successfully in three 30-1 jar fermentors. By reproducing these conditions in a 3-$m^3$ fermentor, we were able to produce HA with a molecular weight of $2.9{\times}10^6$ at a productivity of 5.4 g/l under large-scale conditions.