• Title/Summary/Keyword: Fermentation liquid

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Utilization of Liquid Waste from Methane Fermentation as a Source of Organic Fertilizer -II. Effect of Liquid Waste on Chemical Components, Digestible Dry Matter and Net Energy of Pasture Mixtures (메탄발효폐액(醱酵廢液)의 비료화(肥料化)에 관(關)한 연구(硏究) -II. 폐액시용(廢液施用)이 목초(牧草)의 화학성분(化學成分), 가소화건물(可消化乾物) 및 Net Energy에 미치는 영향(影響))

  • Shin, Jae-Sung;Kim, Jeong-Gap;Lim, Dong-Kyu;Han, Ki-Hak
    • Korean Journal of Soil Science and Fertilizer
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    • v.20 no.2
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    • pp.147-151
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    • 1987
  • This study was conducted to evaluate the effect of the liquid waste from methane fermentation on chemical components, digestible dry matter and net energy of pasture mixtures of orchard grass, tall fescue, Kentucky bluegrass and ladino clover. The total crude proteins and crude ashes increased with the application of liquid waste, but N-free extracts decreased somewhat and it didn't affect the content of crude fat and crude fiber. Amounts of neutral detergent fiber and acid detergent fiber increased with increasing application of liquid waste and the content of cellulose in cell-wall constituents decreased, but hemicellulose increased. The digestible dry matter was produced 405 kg/10a at optimum application of 42 MT/10a liquid waste compared to NPK check plot of 233 kg/10a. The starch value and net energy lactation were 291.3 KStE and 3450 MJ-NEL respectively indicating that the optimum application of liquid waste increased their values.

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Effect of Fermentation Temperature and Salt Concentration on the Fermentation Rate of Dongchimi (발효온도와 소금농도가 동치미 발효속도에 미치는 영향)

  • Chang, Sang-Keun;Ko, Soon-Nam;Choi, Hee-Sook;Kim, Jong-Goon;Kim, Woo-Jung
    • Applied Biological Chemistry
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    • v.39 no.5
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    • pp.398-402
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    • 1996
  • This study was carried out to compare the fermentation rates of Dongchimi as affected by fermentation temperature and salt concentration of brining solution. The Dongchimi was prepared by fermentation of the radish in salt solutions of 2.0, 3.2 and 5.1% NaCl respectively with addition of garlic, green onion, ginger at $4^{\circ}C,\;10^{\circ}C,\;20^{\circ}C$. The fermentation was classified into 3 steps of initial, intermediate and final stages according to pH changes and the rates of fermentation and color change were calculated from intermediate stage. The time reached equilibrium in hardness of radish and salt concentration and their equilibrated values were also compared. Fermentation rate became more rapid as fermentation temperature and salt concentration increased. The rates of Hunter color change of Dongchimi liquid were increased as the temperature increased. The 'L' values decreased slowly, and 'a' and 'b' values increased rapidly to maximum at the intermediate stage. The time required to reach equilibrium was reduced for both salt concentration and hardness of radish as the fermentation temperature increased. The equilibrated values of salt concentration were increased while those of hardness of radish were decreased as the temperature and salt concentration increased. The data obtained can be used for prediction of some of the major characteristics of Dongchimi during fermentation.

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The Physico-chemical Changes and Sensory Characteristics of Kimchi Added with the Mashed Red Pepper (마쇄고추를 첨가한 김치의 이화학적 성분 변화 및 관능적 특성)

  • Hwang, Sung-Yeon;Park, So-Hee;Kang, Geun-Ok;Lee, Hyun-Ja;Bok, Jin-Heuing
    • Journal of the Korean Society of Food Culture
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    • v.20 no.2
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    • pp.221-231
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    • 2005
  • We investigated the physico-chemical and sensory characteristics of Kimchi made with red pepper that was washed and mashed. The pH of juice from Kimchi made with red pepper powder was the highest on the day of Kimchi preparation. In the case of Kimchi made with mashed red pepper, the pH of juice and liquid was lower than that of other samples. A similar decrease in pH of juice and liquid was observed up to the second week of fermentation, but the significant difference between both samples wasn't found. The total acidity of Kimchi with mashed red pepper was significantly increased during early fermentation, but was similar during the second week, compared with that of Kimchi with red pepper powder. From the third week of storage, both juice and liquid from Kimchi made with red pepper powder was relatively increased. L and a value of liquid was highest in the case of Kimchi made with mashed red pepper, but b value was lowest during fermentation. In the case of organic acids, acetic acid and lactic acid contents were increased in Kimchi made with mashed red pepper while fermentation progressed. In addition, citric acid content was constant up to the second week in Kimchi made with mashed red pepper but from the third week wasn't detected in both Kimchi made with mashed red pepper and Kimchi made with red pepper powder. In the case of QDA(Quantitative Descriptive Analysis) profiles, the values of Kimchi made with mashed red pepper were significantly higher than those of Kimchi made with red pepper powder in respect to redness, pungency and fresh flavor immediately after the preparation of Kimchi and during the second week of fermentation, but during the fifth week the values were higher in respect to redness and fresh flavor of Kimchi. Appearance and overall acceptability was remarkably increased in Kimchi made with mashed red pepper, compared with that of Kimchi made with red pepper powder immediately after pickling, during the second and the fifth week of fermentation. Therefore, these results indicate that mashed red pepper increased more citric acid content, L and a value of Kimchi in comparison with red pepper powder, resulting in the good effects on overall acceptability due to the significant increase of redness and fresh flavor.

Effects of Feeding Solid-state Fermented Rapeseed Meal on Performance, Nutrient Digestibility, Intestinal Ecology and Intestinal Morphology of Broiler Chickens

  • Chiang, G.;Lu, W.Q.;Piao, X.S.;Hu, J.K.;Gong, L.M.;Thacker, P.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.2
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    • pp.263-271
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    • 2010
  • This trial was conducted to determine the effects of feeding a diet containing solid-state fermented rapeseed meal on performance, nutrient digestibility, intestinal ecology and intestinal morphology of broiler chickens. A mixed liquid culture, containing approximately 5 log cfu/ml Lactobacillus fermentum, Enterococcus faecium, Saccharomyces cerevisae and Bacillus subtilis was prepared in a 1:1:1:1 ratio. A basal substrate (BS) containing 75% rapeseed, 24% wheat bran and 1% brown sugar was mixed with the liquid culture in a ratio of 10:3. Over the 30-day fermentation, isothiocyanates were reduced from 119.6 to 14.7 mmol/kg. A total of 168, day-old male Arbor Acres broiler chicks were assigned to one of three dietary treatments including a corn-soybean meal based control diet as well as two experimental diets in which the control diet was supplemented with 10% of the BS containing unfermented rapeseed meal or 10% of the BS containing rapeseed meal subjected to solid state fermentation. There were 8 pens per treatment and 7 birds per pen. From days 19-21 and days 40-42, uncontaminated excreta were collected from each pen for digestibility determinations. In addition, digesta from the colon and ceca were collected to determine the number of lactobacilli, enterobacteria and total aerobes. The middle sections of the duodenum, jejunum, and ileum were collected for intestinal morphology. Over the entire experimental period (d 1-42), the weight gain and feed conversion of birds fed fermented rapeseed meal were superior (p<0.05) to that of birds fed nonfermented rapeseed meal and did not differ from the soybean control. On day 42, birds fed fermented rapeseed meal had higher (p<0.05) total tract apparent digestibility coefficients for dry matter, energy, and calcium than birds fed non-fermented rapeseed meal. Colon and ceca digesta from broilers fed the fermented feed had higher (p<0.05) lactobacilli counts than birds fed the control and non-fermented rapeseed meal diets on day 21 and 42. Fermentation also improved (p<0.05) villus height and the villus height:crypt depth ratio in the ileum and jejunum on day 21 and 42. The results indicate that solid-state fermentation of rapeseed meal enhanced performance and improved the intestinal morphology of broilers and may allow greater quantities of rapeseed meal to be fed to broilers potentially reducing the cost of broiler production.

Modelling of the Heat and Mass Transfer in a Liquid Desiccant Dehumidifier with Extended Surface (확장표면을 적용한 액체식 제습기의 열물질 전달 모델링)

  • Chang, Y.S.;Lee, D.Y.
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.23 no.4
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    • pp.303-311
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    • 2011
  • This study presents a new idea of liquid desiccant dehumidifier with extended surface to improve the compactness. Extended surface is inserted between vertical cooling tubes, and the liquid desiccant flows down along the tube walls and the extended surface as well. Though the extended surface contributes to the increase in the mass transfer area, the effect tends to be limited because less conductive non-metallic materials need to be applied due to the high corrosiveness of liquid desiccant. To analyze the effects of the extended surface insertion, mathematical modelling and numerical integration are performed for the heat and mass transfer in the liquid desiccant dehumidifier. The results show that, though the liquid desiccant on the extended surface is heated due to the moisture absorption, the temperature can be maintained by periodic mixing at the contact points between the tube and the extended surface with the liquid desiccant stream from the tube side at a relatively low temperature. This implies the absorption heat from the extended surface side can be removed effectively by mixing, which leads to a substantial improvement of the dehumidification in the liquid desiccant dehumidifier with extended surface. When the interval of the extended surface, $p_e/L$, is less than 0.1, the dehumidification is shown to increase by more than two times compared with that without extended surface.

Quality Characteristics of Wheat Flour Breads with the Doughs Frozen at the Different Freezing and Storage Conditions (반죽의 냉동과 저장 조건에 따른 빵의 품질 특성)

  • Koh, Bong-Kyung
    • Korean Journal of Food Science and Technology
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    • v.34 no.3
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    • pp.413-418
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    • 2002
  • The dough was frozen either before or after fermentation at the five different freezing and storage conditions. Although fermentation before freezing was effective for rapid freezing, it reduced bread volume of the dough frozen at both air freezer and liquid immersion freezer. Freezing at the air freezer set to $-70^{\circ}C$ took more time for freezing and resulted in lower bread volume than freezing at the immersion freezer set to $-20^{\circ}C$. Therefore, the freezing in the liquid immersion freezer was more effective to reduce the freezing time and increase the bread volume. At the liquid immersion freezer, the higher temperature was more effective than lower temperature. The doughs frozen in a liquid immersion freezer set to $-10^{\circ}C$ and fermented after de-frosting produced higher bread volume than control unfrozen dough. And also there was no significant difference in bread volume between the control unfrozen dough and the dough frozen in a liquid immersion freezer set to $-10^{\circ}C$, fermented before freezing and re-fermented after defrosting. The longer proof time and greater loaf volume obtained for the dough frozen and stored at the air freezer set to $-70^{\circ}C$. Therefore the optimum process for freezing the dough was freezing immediately after mixing, storing at $-10^{\circ}C$ in a liquid immersion freezer and fermented after defrosting.

Characteristics of Fermented Hot Pepper Soybean Paste (Kochujang) Prepared by Liquid Beni-koji (액체홍국코지를 이용한 고추장의 제조)

  • Kang, Seong-Gook;Park, In-Bae;Jung, Soon-Teck
    • Korean Journal of Food Science and Technology
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    • v.29 no.1
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    • pp.82-89
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    • 1997
  • Monascus anka produces biologically active materials, and liquid M. anka koji was employed for preparing fermented hot pepper soybean paste (kochujang). Three kinds of koji (M. anka, Aspergillus oryzae and mixed koji) were used to prepare kochujang, and changes of physicochemical characteristics were examined during fermentation. A. oryzae koji showed the highest ${\alpha}-amylase$ and protease activities; whereas, M. anka koji showed the highest ${\beta}-amylase$ activity in liquid koji. Water content of kochujang continuously decreased, whereas viscosity increased during fermentation. The water content and viscosity was 46% and $1.4{\times}10^5\;cP$, respectively after 40 days of fermentation. The pH of kochujang with M. anka and/or A. oryzae koji was $4.63{\sim}4.65$ in the beginning and was between 4.53 and 4.67 after 40 days of fermentation. L-, a- and b-values decreased rapidly during fermentation until 20 days of fermentation. After 40 days, L- and b-values of kochujang prepared with M. anka koji showed lower values than A. oryzae, and a-value showed higher values as the amount of M. anka koji increased. Ammoniacle nitrogen content continuously increased during fermentation. Amino nitrogen content was the highest, 241.2 ㎎%, and ammoniacle nitrogen was the lowest, 97.2 ㎎%, in kochujang prepared with A. oryzae. However, amino and ammoniacle nitrogen content showed no difference between M. anka and A. oryzae koji kochujang. Kochujang prepared with M. anka koji showed a potential that kochujang can be manufactured lower level of red pepper than previously used.

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Analysis Screening of Bioconversion Products from Fermentation Samchulgeonbi-tang with Microorganisms via RP-HPLC-DAD (RP-HPLC-DAD를 이용한 발효 삼출건비탕의 생물전환 성분 분석)

  • Lee, Kwang Jin;Yang, Hye Jin;Liang, Chun;Ma, Jin Yeul
    • Korean Journal of Pharmacognosy
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    • v.44 no.2
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    • pp.125-130
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    • 2013
  • Samchulgeonbi-tang (SC) is well-known traditional herbal medicine which is composed of fourteen medicine herbs. SC has been used for the treatment of the chronic gastritis, indigestion, gastric ulcers, gastroptosis and diarrhea disease. The variation in the amount of bioactive components of SC and its fermentation SC with ten Lactobacillus strains were investigated via high-performance liquid chromatography coupled with diode array detection (HPLC-DAD). Simultaneous qualitative and quantitative analysis of seven bioactive compounds; paeoniflorin, liquiritin, hesperidin, liquiritigenin, kaempferol, atractylenolide III, magnolol were achieved by comparing their retention times ($t_R$) and UV spectra with those of the standard compounds. In the results, the amount of paeoniflorin and hesperidin were 7.967 mg/g, 7.251 mg/g that were the main compounds in SC. The amounts of liquiritigenin was increased by all ten Lactobacillus strains, except strain 128. Especially, the liquiritigenin amount was highest in SC fermented with strain 145 (0.201 mg/g), which was increased by 158.39% compared to SC (0.081 mg/g). In the fermented SC using strains 344, almost components were increased than non-fermented SC, except paeoniflorin and kaempferol. Thus, these results considered that the strains 145 and 344 are most excellent fermentation strains among the 10 species of fermentation strains.

Mass Spectrometry-Based Metabolite Profiling and Bacterial Diversity Characterization of Korean Traditional Meju During Fermentation

  • Lee, Su Yun;Kim, Hyang Yeon;Lee, Sarah;Lee, Jung Min;Muthaiya, Maria John;Kim, Beom Seok;Oh, Ji Young;Song, Chi Kwang;Jeon, Eun Jung;Ryu, Hyung Seok;Lee, Choong Hwan
    • Journal of Microbiology and Biotechnology
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    • v.22 no.11
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    • pp.1523-1531
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    • 2012
  • The metabolite profile of meju during fermentation was analyzed using mass spectrometry techniques, including GC-MS and LC-MS, and the bacterial diversity was characterized. The relative proportions of bacterial strains indicated that lactic acid bacteria, such as Enterococcus faecium and Leuconostoc lactis, were the dominant species. In partial least-squares discriminate analysis (PLS-DA), the componential changes, which depended on fermentation, proceeded gradually in both the GC-MS and LC-MS data sets. During fermentation, lactic acid, amino acids, monosaccharides, sugar alcohols, and isoflavonoid aglycones (daidzein and genistein) increased, whereas citric acid, glucosides, and disaccharides decreased. MS-based metabolite profiling and bacterial diversity characterization of meju demonstrated the changes in metabolites according to the fermentation period and provided a better understanding of the correlation between metabolites and bacterial diversity.

Effect of Sample Preparation on Prediction of Fermentation Quality of Maize Silages by Near Infrared Reflectance Spectroscopy

  • Park, H.S.;Lee, J.K.;Fike, J.H.;Kim, D.A.;Ko, M.S.;Ha, Jong Kyu
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.5
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    • pp.643-648
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    • 2005
  • Near infrared reflectance spectroscopy (NIRS) has become increasingly used as a rapid, accurate method of evaluating some chemical constituents in cereal grains and forages. If samples could be analyzed without drying and grinding, then sample preparation time and costs may be reduced. This study was conducted to develop robust NIRS equations to predict fermentation quality of corn (Zea mays) silage and to select acceptable sample preparation methods for prediction of fermentation products in corn silage by NIRS. Prior to analysis, samples (n = 112) were either oven-dried and ground (OD), frozen in liquid nitrogen and ground (LN) and intact fresh (IF). Samples were scanned from 400 to 2,500 nm with an NIRS 6,500 monochromator. The samples were divided into calibration and validation sets. The spectral data were regressed on a range of dry matter (DM), pH and short chain organic acids using modified multivariate partial least squares (MPLS) analysis that used first and second order derivatives. All chemical analyses were conducted with fresh samples. From these treatments, calibration equations were developed successfully for concentrations of all constituents except butyric acid. Prediction accuracy, represented by standard error of prediction (SEP) and $R^2_{v}$ (variance accounted for in validation set), was slightly better with the LN treatment ($R^2$ 0.75-0.90) than for OD ($R^2$ 0.43-0.81) or IF ($R^2$ 0.62-0.79) treatments. Fermentation characteristics could be successfully predicted by NIRS analysis either with dry or fresh silage. Although statistical results for the OD and IF treatments were the lower than those of LN treatment, intact fresh (IF) treatment may be acceptable when processing is costly or when possible component alterations are expected.