• Title/Summary/Keyword: Fermentation condition

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Optimal Conditions for Propagation in Bottom and Top Brewing Yeast Strains

  • Cheong, Chul;Wackerbauer, Karl;Lee, Si-Kyung;Kang, Soon-Ah
    • Food Science and Biotechnology
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    • v.17 no.4
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    • pp.739-744
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    • 2008
  • The method of yeast propagation has an influence on yeast physiology, fermentation ability, flocculation rate, and taste stability of beer. In order to find optimal conditions for propagation, several parameters were investigated in combinations. The bottom brewing yeast grown at $10^{\circ}C$ indicated that a higher flocculation capacity during the $1^{st}$ fermentation. However, the taste stability and the aroma profile were not affected by parameters of propagation investigated. The beer quality was rather affected by storage duration. In addition, a correlation between tasting and chemiluminescence was found at the beer, which was produced using bottom brewing yeast. The propagation at $10-25^{\circ}C$ with addition of zinc ion indicated the best condition to improve fermentation ability, flocculation rate, and filterability for bottom brewing yeast, whereas the propagation at $30^{\circ}C$ with addition of zinc ion showed the best condition to increase fermentation ability for top brewing yeasts.

D(-) and L(+)-Lactic Acid Determination of Lactobacillus acidophilus during Fermentation and Storage Period (Lactobacillus acidophilus NCFM의 배양 및 저장 중 D(-) 및 L(+)-lactic acid의 변화)

  • Lee, Kyung-Wook;Shin, Yong-Kook;Baick, Seung-Chun
    • Korean Journal of Food Science and Technology
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    • v.30 no.1
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    • pp.168-174
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    • 1998
  • The amount of D(-)-lactic acid in fermented dairy products is very important because the rate of metabolism of D(-)-lactic acid is lower than that of L(+)-lactic acid. The purpose of this study was to investigate the optimum condition during fermentation and storage of yogurt for the formation of isomers of lactic acid by Lactobacillus acidophilus NCFM. The production of acid was excellent at $37^{\circ}C$ of fermentation and the ratio of D(-)-lactic acid was also lower than that of other conditions such as $35^{\circ}C{\;}and{\;}40^{\circ}C$. Among shaking and non-shaking treatment under aerobic condition and anaerobic condition, non-shaking treatment under aerobic condition was the best condition at the production of acid and L(+)-lactic acid during fermentation. During storage at low temperature, a larger amount of L(+)-lactic acid was produced than at higer storage temperature.

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Optimal Condition for Deacidification Fermentation of Wild Grape Wine by Mixed Culture (혼합배양에 의한 산머루주의 감산발효 최적조건)

  • Kim, Seong-Ho
    • Applied Biological Chemistry
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    • v.51 no.1
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    • pp.17-23
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    • 2008
  • In order to prevent wine quality deterioration caused by strong sour taste from raw and other materials during fermentation of wild grape wine, the various mixed cultures conditions of the deacidification fermentation and the alcohol fermentation process by inoculation of mixed strains were investigated. As a result of mixed cultures process after the inoculation of Schizosaccharomyces pombe and Schizosaccharomyces japonicus with each deacidification fermentation strain in a culture of Saccharomyces sp. SMR-3 which was used in the alcohol fermentation strain of wild grape, cultures for 12 days at $22^{\circ}C$ with Saccharomyces sp. SMR-3 and Schizosaccharomyces pombe resulted in the maximum alcohol content at $15.8{\pm}0.2%$ and the minimum with the acidity of $0.44{\pm}0.02%$, the total organic acid of $648.96{\pm}7.14$ mg% and malic acid of $99.30{\pm}1.24$ mg%. Mixed cultures with Saccharomyces sp. SMR-3 and Schizosaccharomyces pombe under the optimal condition for the deacidification fermentation of wild grape wine showed 2% higher alcohol content, 51.65% lower acidity, 48.02% lower total organic acid, and 81.12% lower malic acid than a single culture of Saccharomyces sp. SMR-3.

Optimization for the DXAMase production from Lipomyces starkeyi using statistically designed experiments

  • Park, Jun-Seong;Gang, Hui-Gyeong;Gang, Seong-Ju;Kim, Byeong-Hun;Park, Don-Hui;Kim, Do-Man
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.211-214
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    • 2002
  • The optimal condition for the production of DXAMase, containing the both characteristics of dextranase and amylase, was studied based on different levels of pH, temperature, and aeration rate. Response surface methodology was applied to find the optimatic condition showing the relationship between the fermentation response(dextranase and amylase activity of DXAMase) and the fermentation variables(pH, temperature, and agitation rate). In case of dextranase activity, the condition of pH 4.06, $28.08^{\circ}C$, and 235.14 rpm showed the highest activity, 2.26 U/ml, and for amylase activity, the condition of pH 4.01, $27.96^{\circ}C$, and 212.01 rpm showed the highest activity, 3.52 U/ml. For the production of DXAMase, dextranase and amylase, the optimum condition was pH 4.06, $28.08^{\circ}C$, and 234.80 rpm.

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Effect of Air Controlled Fermentation on Kimchi Quality (밀폐용기에서의 김치숙성에 관한 연구)

  • 김미경;김소연;우철주;김순동
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.2
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    • pp.268-273
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    • 1994
  • The quality of Kimchi was investigated under airtight and ventilating conditions. At 2$0^{\circ}C$ and 4$^{\circ}C$ fermentation, there were no significantly difference in pH, acidity and vitamin C of Kimchi between airtight and ventilating conditions. The contents of $CO_2$ , the number of lactic acid bacteria and the sensory score of carbonated taste under airtight condition were higher than those values under ventilating condition. While , the number of aerobic bacteria under airtight condition was lower than those under ventilating condition.

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Repeated Fed-Batch Fermentation of Wheat Flour Solution by Mixed Lactic Acid Bacteria (혼합 젖산균을 이용한 밀가루 용액의 반복 유가식 발효)

  • Kim, Sang-Yong;Noh, Bong-Soo;Oh, Deok-Kun
    • Korean Journal of Food Science and Technology
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    • v.29 no.2
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    • pp.343-347
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    • 1997
  • Effect of culture conditions on the fermentation of wheat flour solution by mixed lactic acid bacteria of Lactobacillus brevis, L. fermentum and L. plantarum was investigated. The optimum temperature for the fermentation of wheat flour solution was $35^{\circ}C$ because pH decreased the lowest value and TTA (total titrable acidity) increased the highest value at this temperature. In aerobic condition, fermentor was purged with air at 1.0 vvm and was purged with nitrogen gas at 1.0 vvm in anaerobic condition. The decrease of pH and the increase of TTA in aerobic condition were higher than those in anaerobic condition. In aerobic condition, the optimum condition of oxygen supply was found to be oxygen transfer rate coefficient of $60\;hr^{-1}$ which corresponded to agitation speed of 250 rpm in a 5 L fermentor. Repeated fed-batch cultures were performed using pH-stat in order to increase the productivity of fermented wheat flour. With increasing the repeated fraction of culture volume, mean cycle time increased but maximum operation time decreased. However, the volume of produced broth per culture volume per time and total volume of produced broth per culture volume were maximum at the repeated fraction of culture volume of 20%. In a repeated fed-batch fermentation of wheat flour solution using mixed lactic acid bacteria, the culture condition was optimum at temerature of $35^{\circ}C$, aeration rate of 1.0 vvm, oxygen transfer rate coefficient of $60\;hr^{-1}$, and repeated fraction of culture volume of 20%.

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A Plan for Improving Quality of Traditional Soybean Paste (전통된장의 품질개선에 관한 연구)

  • 최동원
    • The Korean Journal of Food And Nutrition
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    • v.16 no.3
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    • pp.218-223
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    • 2003
  • This study is proceeded on the development of standard method for making soybean paste by Korean traditional method. Fermentation condition of Meju was 1) Pre-fermentation : 30 days in about 20$^{\circ}C$ room, 2) Main fermentation : 5 days in 30$^{\circ}C$ or upper temperature, 3) Post fermentation and drying : 30 days in well sunlightened room in January. Meju was soaked in 18% salt solution(Meju 7kg/salt solution 20L) for 35~40 days (from late February to early April) and after soaking Meju was filtered as unsoluble solute and crushed and put into traditional Korean receptacle(named 'Dok'). Crushed Meju was stored from early April to mid September and Meju was changed into soybean paste(Doen-jang). During fermentation amino acid nitrogen in Doen-jang was slightly increased in early period and decreased lately. It has been proved that by panel test soybean paste made by the method suggested in this study was more excellent than commercially fermented soybean paste. This study has presented the possibility of commercial production of soybean paste made by traditional method.

Sensory and Microbiological Properties of Dongchimi added with Gatt (Brassica juncea) (갓 동치미의 관능적 및 미생물학적 특성)

  • 박정은;김형렬;장명숙
    • Korean journal of food and cookery science
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    • v.16 no.1
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    • pp.57-64
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    • 2000
  • An optional ingredient, Gatt(Brassica juncea) was adopted to improve the quality of Dongchimi during fermentation. The final weight percentage of Gatt in Dongchimi was adjusted to 0, 3, 5, 10, or 15%, per radish. Sensory and microbiological characteristics were determined during fermentation at 10$^{\circ}C$ for 45 days. The effect was varied depending on the amounts of Gatt, but Dongchimi fermented with 5% Gatt was most favored for color, flavor, taste, and overall acceptability in sensory evaluation. According to the quantitative descriptive analysis for the product, the liquid portion of Dongchimi steadily became clearer and less sour in proportion to the amount of added Gatt. The fermentation retarding action of Gatt at the initial stage of Dongchimi fermentation was clearly seen as above. However, the addition of Gatt at above 15% of Chinese radish was not desirable due to the accelerated fermentation at the later stage. The general changing patterns in typical characteristics of fermentation including microbial counts were comparable with each other depending on the treatments. And favorable Dongchimi could be prepared by fermenting with 5% Gatt at the given condition.

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Acid Fermentation Characteristic of Food Wastes According to the Organic Loading Rate (유기물부하에 따른 음식물찌꺼기의 산발효 특성)

  • Park, Jin-Sik;Ahn, Chul-Woo;Jang, Seong-Ho
    • Journal of Environmental Science International
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    • v.15 no.10
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    • pp.975-982
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    • 2006
  • This study has been conducted to optimum operating conditions for effective acid fermentation according to OLR(organic loading rate) in the mesophilic and thermophilic acid fermentation process. The results are summarized as follows. In order to obtain reasonable acid fermentation efficiency in performing acid fermentation of food wastes in thermophilic condition, organic loading rate was required below 20 gVS/L.d. As $SCOD_{Cr}/TKN,\;SCOD_{Cr}/T-P$ of thermophilic acid fermented food wastes In organic loading rate 20 gVS/L.d were 18.9, 73.4 respectively, it was possible to utilize as external carbon source for denitrification in sewage treatment plant after solid-liquid separation as well as co-digestion of fermented food wastes and sewage sludge.

A Study on the Physicochemical Properties Alteration of Aloe Saponaria Fermentation

  • Kweon, Do-Yeong;Kang, Min-Woo;Park, Jong-Min;Kim, Jong-Soon;Kwon, Soon-Goo;Choi, Won-Sik
    • Journal of the Korean Society of Industry Convergence
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    • v.23 no.6_1
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    • pp.897-906
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    • 2020
  • This research focuses on the physicochemical characteristic of fermentation of Aloe Saponaria. The fermentation process applied in this study had 2 variation, depends on the aloe part as the materials (bottom, middle, and tip) and the initial sugar content (24% and 0%) used. Tests are conducted using uinkin fermented powder, sugar, salt, and distilled water as fermenting agent. The results indicate that change in physicochemical properties of aloe's skin was larger than in aloe's gel as fermentation materials. In contrast, there was no significant change in aloe's leaf during the process. A lso, aloes with intial sugar condition of 24% show better results than which without sugar addition in fermentation.