• Asian Pacific Journal of Cancer Prevention
• /
• 제14권2호
• /
• pp.1017-1021
• /
• 2013

The cellular apoptosis susceptibility (CSE1L) gene has been demonstrated to regulate multiple cellular mechanisms including the mitotic spindle check point as well as proliferation and apoptosis. However, the importance of CSE1L in human colon cancer is largely unknown. In the present study, we examined expression levels of CSE1L mRNA by semiquantitative RT-PCR. A lentivirus-mediated small interfering RNA (siRNA) was used to knock down CSE1L expression in the human colon cancer cell line RKO. Changes in CSE1L target gene expression were determined by RT-PCR. Cell proliferation was examined by a high content screening assay. In vitro tumorigenesis was measured by colony-formation assay. Cell cycle distribution and apoptosis were detected by flow cytometric analysis. We found CSE1L mRNA to be expressed in human colon cancer cells. Using a lentivirus based RNAi approach, CSE1L expression was significantly inhibited in RKO cells, causing cell cycle arrest in the G2/M and S phases and a delay in cell proliferation, as well as induction of apoptosis and an inhibition of colony growth capacity. Collectively, the results suggest that silencing of CSE1L may be a potential therapeutic approach for colon cancer.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권2호
• /
• pp.963-968
• /
• 2013

Objective: To study the effect of the antagomiR-27a inhibitor on glioblastoma cells. Methods: The miR-27a expression level in specimens of human glioblastoma and normal human brain tissues excised during decompression for traumatic brain injury was assessed using qRT-PCR; The predicted target gene of miR-27a was screened out through bioinformatics databases, and the predicted gene was verified using genetic report assays; the effect of antagomiR-27a on the invasion and proliferation of glioma cells was analyzed using MTT assays and 5-ethynyl-2'-deoxyuridine (EdU) labeling. A xenograft glioblastoma model in BALB-c nude mice was established to detect the effect of antagomiR-27a on tumour growth. Results: qRT-PCR results showed that miR-27a significantly increased in specimens from glioblastoma comparing with normal human brain tissues. Th miR-27a inhibitor significantly suppressed invasion and proliferation of glioblastoma cells. FOXO3a was verified as a new target of miR-27a by Western blotting and reporter analyzes. Tumor growth in vivo was suppressed by administration of the miR-27a inhibitor. Conclusion: MiR-27a may be up-regulated in human glioblastoma, and antagomiR-27a could inhibit the proliferation and invasion ability of glioblastoma cells.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권9호
• /
• pp.5311-5316
• /
• 2013

A functional polymorphism in the NQO1 gene, featuring a 609C>T substitution,leading to proline to serine amino-acid and enzyme activity changes, has been implicated in cancer risk. However, individually published investigations showed inconclusive results, especially for leukemia. In this study, we therefore performed a meta-analysis of 21 publications with a total of 3,634 cases and 4,827controls, mainly for leukemia. We summarized the data on the association between the NQO1 609C>T polymorphism and risk of leukemia and performed subgroup analyses by ethnicity and leukemia type. We found that the variant TT homozygous genotype o was associated with a modestly increased risk of leukemia (TT versus CT/CC: OR=1.23, 95%CI=1.00-1.51, heterogeneity=0.76; $I^2$=0%). Following further stratified analyses, increased risk was only observed in subgroups of Caucasians. This meta-analysis suggests that the NQO1 609T allele is a high-penetrance risk factor for leukemia in Caucasians. The effect on leukemia may be modified by ethnicity and leukemia type, and the small sample sizes of the subgroup analyses suggest that further larger studies are needed.

• Journal of Microbiology and Biotechnology
• /
• 제20권11호
• /
• pp.1471-1480
• /
• 2010

An extremely thermostable xylanase gene, xynB, from the hyperthermophilic bacterium Thermotoga maritima MSB8 was successful expressed in Kluyveromyces lactis. The response surface methodology (RSM) was also applied to optimize the medium components for the production of XynB secreted by the recombinant K. lactis. The secretion level (102 mg/l) and enzyme activity (49 U/ml) of XynB in the optimized medium (yeast extract, lactose, and urea; YLU) were much higher than those (56 mg/l, 16 U/ml) in the original medium (yeast extract, lactose, and peptone; YLP). The secretory efficiency of mature XynB was also improved when using the YLU medium. When the mRNA levels of 13 characterized secretion-related genes in the K. lactis cultured in YLP and YLU were detected using a semiquantitative RT-PCR method, the unfolded protein response (UPR)-related genes, including ero1, hac1, and kar2, were found to be up-regulated in the K. lactis cultured in YLU. Therefore, the nutrient ingredients, especially the nitrogen source, were shown to have a significant influence on the XynB secretory efficiency of the host K. lactis.

• 폴리머
• /
• 제35권2호
• /
• pp.130-135
• /
• 2011

전자 기기의 빠른 발전에 따른 무선 기기들의 사용은 급격히 늘어나고 있다. 그래서 이런 제품에 자가 발전이 가능한 재료를 적용시키는 사례가 점차 늘고 있다. 여기에 사용되는 재료로서 poly(vinylidene fluoride) (PVDF)가 있는데 PVDF는 piezoelectricity를 낼 수 있는 특별한 결정구조인 ${\beta}$-phase를 가지고 있다. 이 논문에서는 piezoelectricity에 결정적인 영향인 ${\beta}$-phase 함량을 증가시키기 위해 다층 PVDF 필름을 제조하였다. 이 PVDF 필름은 용매인 DMAc에 10%로 용해시킨 깃으로 spin rate는 850 rpm, spin time은 60초이며 건조온도는 $60^{\circ}C$이다. 비교적으로 다층 필름은 단열 층보다 더 높은 ${\beta}$-phase함량을 나타내었다 이 ${\beta}$-phase함량은 4-layer 필름이 되기까지 점차 증가되었으며 최대 함량은 7.72이다.

• 한국정보전자통신기술학회논문지
• /
• 제8권6호
• /
• pp.491-496
• /
• 2015

클라우드 컴퓨팅은 서버의 추가 구축에 대한 비용절감, 데이터 스토리지 확대에 대한 비용 절감, 컴퓨터 자원에 대한 공유, 새로운 기술의 적용에 대한 편의성 등의 장점을 가지고 있다. 그러나 서비스 모델의 다양성으로 인하여 새로운 보안의 우려사항이 높아지고 있어, 이용자가 서비스를 이용 시 안전한 사용자 인증방법이 요구되고 있다. 이에 본 논문에서는 클라우드 보안 영역 접근시 향상된 에이다부스트 알고리즘을 활용한다. 에이다부스트는 20도 이상 기울어진 얼굴을 인식 못 한다는 단점에도 불구하고, 속도와 신뢰성이 높다는 장점 때문에 많이 사용되고 있다. 제안된 방법을 이용하면, 실험결과에서 보듯 20도 이상 기울어진 얼굴도 인식함을 확인하였다. 연구용으로 이용할 수 있는 FEI Face Database를 이용하여 알고리즘 수행 결과 98%의 성공률을 얻었다. 실패한 2%는 안경을 쓴 사진이나 다른 객체로 인하여 인식이 제대로 안된 경우이다.

• Corrosion Science and Technology
• /
• 제6권5호
• /
• pp.257-260
• /
• 2007

In order to further improve the corrosion resistance and wear resistance of the Ni-P coatings of electroless plating, electroless Ni-P/n-$Al_2O_3$ composite deposits were prepared by adding some nano $Al_2O_3$ Particles in Ni-P plating bath. The bath composition and proproties were studied in this paper. The orthogonal test was applied in order to get the new composite solution, taking the initial stable potential as evaluation standard and considering the elements correlation at the same time. The processing parameters have been optimized by single factor experiment in which the depositing speed was chosen as the evaluation standard. The results showed that the process is stable and the composite Ni-P/n-$Al_2O_3$ deposits werebright and smooth, whose hardness and corrosion resistance are much better than simple Ni-P coatings. Furthermore the surface appearance and structure of the composite Ni-P/n-$Al_2O_3$ coating were investigated by SEM and XRD method. It was proved that the coating surface is typical cystiform cells and its structure is amorphous. All test results ofcomposite coating showed that all various physical coating properties had been improved by adding nano-particles. The hardness of optimal coating is more than 600HV and increases to 1000HV after heat-treating, and its hardness is 20~50% higher than Ni-P coating. The rust points appeared in 200 hour by immersing the coating into the 10%HCl solution and the corrosive speed is $3{\times}10^{-3}mg/(cm^2{\cdot}h)$which was obtained after 300 hour. In the same condition Ni-P coating is $5.6{\times}10^{-3}mg/(cm^2{\cdot}h)$. The salt spray resistance of the layers can exceed 600h with the thickness $20{\mu}m$.

• Journal of Korea Trade
• /
• 제23권5호
• /
• pp.27-44
• /
• 2019

Purpose - The purpose of this paper is to analyze the net effect of the green total factor productivity (GTFP) of China's manufacturing industry from the China-Korea Free Trade Area (China-Korea FTA) quantitatively. Design/methodology - Firstly, the Global Malmquist-Luenberger (GML) index based on the SBM directional distance function is used to measure the GTFP of China's manufacturing and analyze the driving force for its growth. Secondly, the regression discontinuity quantitative analysis is used to determine the impact of the China-Korea FTA on China's manufacturing GTFP. Findings - Our main findings can be summarized as follows: the China-Korea FTA has promoted the GTFP of China's manufacturing with an effect evaluation mainly resulting from green technology progress. And there is industry heterogeneity in the policy effect on the manufacturing GTFP due to the China-Korea FTA. Namely, policy promotion from the China-Korea FTA is more effective on the GTFP of equipment manufacturing than it is on those of other industries. Originality/value - First, an evaluation and analysis of the GTFP development of China's manufacturing that employs GML index based on SBM directional distance function. Second, a quantitative estimate of China-Korea FTA's net effect on China's manufacturing industrial GTFP that uses regression discontinuity analysis, which is considered to be the closest method to natural experiments and superior to other causal inference methods. Third, an in-depth discussion of the practical steps that China's manufacturing can take to improve GTFP development and integrate China-Korea FTA construction into economic development.

• 국제지역연구
• /
• 제16권3호
• /
• pp.27-51
• /
• 2012

신고전주의 학자들은 한국의 산업화 과정이 '노동잉여' 모델에 따르고 있다고 주장해 왔다. 이 모델은 개발도상국들의 경제발전은 무한한 값 싼 노동력이 제조업 부문으로 이전되면서 진행되고, 따라서 산업화의 성공을 위해서는 노동집약적 기술이 사용되어야 한다는 점을 강조하고 있다. Fei와 Ranis는 일본의 초기 산업화 단계(1887-1915)에서 일본이 노동집약적 기술을 사용하였고 이는 일본의 경제발전을 이끌었다고 주장한다. 이에 본 논문은 한국 산업화 과정이 과연 이 모델에 기인하고 있는 지를 밝히고자 하는데 목적이 있다. 이의 검증을 위하여 Kelly와 Williamson이 고안한 신고전주의 이중모델의 기본 틀이 사용되었다. 이 모델 하에서 연산일반균형 (CGE) 방식을 통해 한국 산업화 기간 중 (1965-1992) 추정된 주요 경제변수와 실제 역사적 수치를 비교해 보았다. 검증 결과 두 수치 간에 큰 차이가 나타남을 확인하였는바 따라서 한국의 산업화 과정은 시장의 힘에 기초한 비교우위 생성의 결과라는 신고전주의 가설이 적용되지 않는다는 결론에 이르게 되었다.

• Parasites, Hosts and Diseases
• /
• 제57권2호
• /
• pp.83-92
• /
• 2019

Based on the reactive oxygen species (ROS) regulatory properties of diphenyleneiodonium (DPI), we investigated the effects of DPI on host-infected T. gondii proliferation and determined specific concentration that inhibit the intracellular parasite growth but without severe toxic effect on human retinal pigment epithelial (ARPE-19) cells. As a result, it is observed that host superoxide, mitochondria superoxide and $H_2O_2$ levels can be increased by DPI, significantly, followed by suppression of T. gondii infection and proliferation. The involvement of ROS in anti-parasitic effect of DPI was confirmed by finding that DPI effect on T. gondii can be reversed by ROS scavengers, N-acetyl-L-cysteine and ascorbic acid. These results suggest that, in ARPE-19 cell, DPI can enhance host ROS generation to prevent T. gondii growth. Our study showed DPI is capable of suppressing T. gondii growth in host cells while minimizing the un-favorite side-effect to host cell. These results imply that DPI as a promising candidate material for novel drug development that can ameliorate toxoplasmosis based on ROS regulation.