• Korean Journal of Poultry Science
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• v.30 no.3
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• pp.169-175
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• 2003

This experiment was conducted to study the optimum energy feeding regimens fur broiler breeders peaked in winter season with 400 caged hens of Ross strain. Four energy supply regimens which were different in daily energy allotment during laying period were employed for 40 weeks from 24 to 64 weeks of age. All experimental diets were formulated to contain 2,750 kcal ME/kg with adjustments made in total feed allotment to provide the desired energy levels. Total consumption of the feed would provide 20 g of protein, 4 g of calcium and 0.35 g of available phosphorus. There were no difference in hen-day egg production and average egg weight among the regimens of energy supply. Feed, ME and feed cost required per egg or per kg egg were significantly increased as the level of energy allotment increased(p<0.05). It was concluded that the energy supply regimen, which supplied 280 kcal ME per day at the age of 24 weeks and then increased the energy supply up to 400 kcal ME per day at the peak period of 30∼34 weeks of age, was superior in feed, ME and feed cost required per egg or per kg egg without any adverse effect on eg production and egg weight.

• Korean Journal of Poultry Science
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• v.30 no.3
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• pp.161-167
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• 2003

A study was conducted to investigate the effect of supplementing earthworm meal (EWM) on the performance of laying hens and fatty acid composition in egg yolks. A total of 360 laying hens at 55 weeks of age were fed the experimental diets containing 0.0% (Control), 0.1% and 0.2% of EWM for 5 weeks. Eggs were collected and weighted every day and egg production and feed conversion were recorded every weeks during the experimental period. However fatty acid composition of egg yolk were measured at last week of experimental period. Amount of feed intake tended to increase by supplemental EMW, but feed conversion ratio of birds fed EWM was not different among three groups. Average egg production seemed to increase and significantly improved (P<0.05) when fed a 0.1% EWM and 0.2% EWM, respectively. Average egg weight was prone to decrease when fed a 0.1% EWM compared to that fed a 0% (control) or 0.2% EWM. Average daily egg mass tended to improve by the addition of EWM. It was more increased in 0.2% EWM treatment than 0.1% EWM. The ratio of egg yolk n-6/ n-3 fatty acids contents was 5:1 fed a 0.1% and 0.2% EWM. But these ratio was 10:1 in control group. It is concluded that 0.2% earthworm meal supplementation in the 55 weeks old laying hens diet, improves the laying performance and ratio of egg yolk n-6/ n-3 fatty acids contents (P<0.05).

• Korean Journal of Poultry Science
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• v.37 no.4
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• pp.313-321
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• 2010

This study was conducted to investigate the effects of dietary supplementation of mulberry leaves and dandelion extracts on pH, meat color, TBARS (thiobarbituric acid reactive substance), and VBN (volatile basic nitrogen). Two hundred broiler chickens were fed diets for five weeks containing 1% mulberry leaves extracts (T1), 2% mulberry leaves extracts (T2), 1% dandelion extracts (T3), and 2% dandelion extracts (T4). At the end of five weeks feeding experiment, broilers were slaughtered, and stored at $4^{\circ}C$ for five weeks. As storage time increased, the presence of mulberry leaves and dandelion extracts resulted in decreased pH, and $L^*$ and increased TBARS, VBN, and $a^*$ value in all treatment groups (P<0.05). The pH value, TBARS, and VBN were significantly decreased by the supplementation of mulberry leaves and dandelion extracts relative to the control (P<0.05). Therefore, mulberry leaves and dandelion extracts had the possibility to improve shelf life of chicken meat. Especially, T4 was significantly more effective in delay lipid oxidation compared to the control group. However, no significantly difference was found in pH, TBARS, and VBN among mulberry leaves and dandelion extracts treatment groups. In conclusion, this study demonstrates that compared to control group, supplementation of mulberry leaves and dandelion extracts were effective in decreasing pH, TBARS, and VBN and increasing $a^*$ value.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1273-1273
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• 2001

The efficiency of the luminal fermentation process influences overall efficiency of luminal production, animal health and reproduction. Ruminant production systems have a significant impact on the global environment, as well. Animal wastes contribute to pollution of the environment as ammonia volatilized to the air and nitrate leached to ground water. Microbial protein synthesis in the rumen satisfies a large proportion of the protein requirements of animals. Quantifying the microbial synthesis is possible by using markers for lumen bacteria and protozoa such as nucleic acids, purine bases, some specific amino acids, or by isotopic $^{15}N,^{32}P,\;and\;^{35}S$ labelled feeds. All those methods require cannulated animals, they are time-consuming and some methods are very expensive as well. Many attempts have been made to find an alternative method for indirect measurement of microbial synthesis in intact animals. The present investigations aimed to assess possibilities of NIRS for prediction of purine nitrogen excretion and ruminal microbial nitrogen synthesis by NIR spectra of urine. Urine samples were collected from 12 growing sheep,6 of them male, and 6- female. The sheep were included in feeding experiment. The ration consisted of sorghum silage and protein supplements -70:30 on dry matter basis. The protein supplements were chosen to differ in protein degradability. The urine samples were collected daily in a vessel containing $60m{\ell}$ 10% sulphuric acid to reduce pH below 3 and diluted with tap water to 4 liters. Samples were stored in plastic bottles and frozen at $-20^{\circ}C$ until chemical and NIRS analysis. The urine samples were analyzed for purine derivates - allantoin, uric acid, xantine and hypoxantine content. Microbial nitrogen synthesis in the lumen was calculated according to Chen and Gomes, 1995. Transmittance urine spectra with sample thickness 1mm were obtained by NIR System 6500 spectrophotometer in the spectral range 1100-2500nm. The calibration was performed using ISI software and PLS regression, respectively. The following statistical results of NIRS calibration for prediction of purine derivatives and microbial protein synthesis were obtained.(Table Omitted). The result of estimation of purine nitrogen excretion and microbial protein synthesis by NIR spectra of urine showed accuracy, adequate for rapid evaluation of microbial protein synthesis for a large number of animals and different diets. The results indicate that the advantages of the NIRS technology can be extended into animal physiological studies. The fast and low cost NIRS analyses could be used with no significant loss of accuracy when microbial protein synthesis in the lumen and the microbial protein flow in the duodenum are to be assessed by NIRS.

• Korean Journal of Poultry Science
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• v.38 no.3
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• pp.239-245
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• 2011

Pigments in the diet affect yolk colors. Due to variations in both the bioavailability of pigments in chickens and their amounts occurring in the feed ingredients, concern about egg quality arises in terms of yolk color. In this study, the effects of pigments, produced through cell culture in the laboratory, on yolk colors were determined for 4 weeks in laying hens receiving one of the 6 dietary treatments: control diets containing 1) no synthetic pigments (CON); 2) canthaxanthin (4 ppm) purchased from BASF (BASF); 3) cultured cells so that the diet had canthaxanthin at 4 ppm (CX); 4) cultured cells so that the diet had lycopene at 30 ppm (LP); 5) canthaxanthin (4 ppm) that was purified from cultured cells (SPCX); or 6) lycopene (30 ppm) that was purified from cultured cells. Relation between deposition of pigments into yolks and egg production was also tested. Yolk color of eggs from chickens fed dietary CX was significantly enhanced, which was slightly but significantly below that of BASF. Results from other treatments were lower than those of CX. Deposit rates of pigments into yolks were: BASF > CX > SPCX > LP > SPLP. The amounts of pigments, with the exception of SPLP, in feed were not changed during the storage for 4 weeks at $25^{\circ}C$. Egg production rates varied among treatments during the initial phase of the study but became relatively uniform at the later stage, except for CON and LP groups. The results of the present study indicate that the deposition of pigments into yolks is independent of egg production.

• Journal of Nutrition and Health
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• v.29 no.1
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• pp.22-31
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• 1996

The purpose of this study was to investigate the effect of vitamin E and selenium on the antioxidative defense mechanism in the liver of streptozotocin(STZ)-induced diabetic rats. Sprague-Dawley male rats(120$\pm$10gm) were randomly assigned to one control and five STZ-diabetic groups. Diabetic groups were classified to STZ-0E (vitamin E free diet), STZ-40E(40mg vitamin E/kg of diet), STZ-400E(400mg vitamin E/kg of diet), STZ-S(0.5ppm Se/kg of diet) and STZ-400ES(400mg vitamin E and 0.5ppm Se/kg of diet) according to the level of vitamin E and selenium supplementation. Diabetes was experimentally induced by intravenous adminstration of 55mg/kg of STZ in citrate buffer(pH 4.3) after 4-weeks feedng of six experimental diets. Animals were sacrificed at the 4th day of diabetic states. Activities of the serum glutamic oxaloacetate transaminase(GOT) and the glutaminc pyruvate transaminase(GPT) in STZ-0E, STZ-40E and STZ-S rats were higher than those of control. Liver xanthine oxidase activities were similar to serum GOT and GPT. Liver superoxide dismutase(SOD) activities were higher in STZ-0E and STZ-40E groups by 33%, 22%, respectively than that of control. Glutathione S-transferase(GST) activities of liver were similar to GSH-Px activities. The contents of vitamin E in liver tissue were significantly lower STZ-0E, STZ-40E and STZ-S groups by 50%, 36%, 45% than that of control. Reduced glutathione(GSH) contents of liver were lower STZ-0E, STZ-40E, STZ-400E, STZ-S and STZ-400ES groups by 57%, 51%, 19%, 18%, 12% than that of control. Lipid peroxide values (LPO) in liver were higher 5.6, 2.3 and 2.3 times in STZ-0E, STZ-40E and STZ-S group than that of control. The present results indicate that STZ-induced diabetic rats are more sensitive to oxidative stress, leading to the acceleration of lipid peroxidation process, which can be more accelerated by feeding the low level of dietary vitamin E. In the coincident supplementation of high dietary vitamin E and selenium antioxidative enzymes activities and physiolosical antioxidants were increased more than those of the separate supplementation of vitamin E or selenium. Therefore, dietary vitamin E and selenium reduced peroxidative damage of tissue, promoting antioxidative defense mechanism against lipid peroxidation by diabetes.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.2
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• pp.319-326
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• 1997

This study was conducted to investigate the effects of methionine(Met) and selenium(Se) levels on alcohol metabolic enzyme system in rats. Sprague-Dawley male rats were fed on diets containing one of the three levels of Met(0, 3, 9g/kg diet) with or without Se(0.45mg/kg diet). Alcohol was administrated with 25%(v/v) ethanol orally at the same time once a day in alcohol group and isocaloric sucrose was administrated to the control group. The rats were sacrificed after 5 and 10 week of feeding periods. Alcohol dehydrogenase(ADH) and microsomal ethanol oxidizing system(MEOS) activities of hepatic tissuedom were increased more in alcohol treated groups than control group. Increment of activities preinated in simultaneous deficiency of dietary Met and Se(LMet-Se+EtOH) group. Aldehyde dehydrogenase (AIDH) activity was decreased more in alcohol treated groups than control group and significantly decreased in Met and Se supplemented(NMet+Se+EtOH) group. Hepatic cytochrome P-450 content and xanthine oxidase(XO) activity were significantly increased in alcohol treated groups Compared to control group and predominated in Met deficiency(LMet) group and excessive Met administration (HMet) group. Superoxide dismutase(SOD), catalase, glutathione S-transferase(GST) activities tended to increase by alcohol administration, the degree of increase predominated in 10 week. The activity of glutathione peroxidase(GSH-Px) was decreased in alcohol groups and tended to increase in proportion to the level of dietary Met.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.27 no.3
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• pp.232-239
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• 1994

In order to determine the utilization of ascidian tunic, which has been blamed for problems of costal environmental pollution when discharged into the sea after being used as a natural dietary pigment sources for rainbow trout(Oncorhynchus mykiss), fingerlings were fed on experimental diets containing acetone-extracts for 6 weeks. The amounts of acetone-extracts were 11,000mg/Kg and contained 50mg/100g wet tissues of carotenoid and $6\%$ of carotenoids were astaxanthin. From the results of feeding experiments, the growth rate in the extract group was a little higher than that of the control and pink groups after 6 weeks. The redness and yellowness of the fish skin and muscle in the extract group were similar to the pink group. Therefore, acetone-extracts of ascidian tunic were judged to be a natural dietary pigment source suitable as a substitute synthetic pigment for aquaculture use.

• Journal of Nutrition and Health
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• v.32 no.6
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• pp.628-636
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• 1999

There is a marked increase in geriatric disease, especially liver disease, due to the continuous increase in alcohol and fat consumption. Since the fatty liver, induced by alcohol or fat, is basically from abnormalities in the lipid metabolism, it is possible that fatty acid binding protein(FABP) which is related to the fatty acid metabolism may also be abnormal in these livers. FABP is a small molecular weight protein family present in cytosol in high concentration. It has been proposed as a fatty acid transfer protein and as a binding protein responsible for controlling intracellular free fatty acid concentration. In this research, we have examined the relationship between liver FABP and fatty liver induced by alcohol or high cholesterol diet. Rats were fed one of either semipurified liquid diets; control diet containing 65% carbohydrate, 20% protein, and 15% fat or high cholesterol diet containing 1%(w/w) cholesterol or alcohol diet containing 37% of alcohol instead of carbohydrate. After 5 weeks of feeding period, all rats received commercial chow diet for 5 weeks to examine recovery effect. Liver and blood samples were collected at 0, 1, 3, 5 and 10 weeks to analyze lipid compositions. FABP was purified from liver cytosol and injected to rabbit to obtain antiserum. Liver FABP amount was determined by SDS-PAGE and western blotting methods. Fatty acid binding capacity was determined by binding of 14Cpalmitate with the delipidated liver cytosol. Consumption of alcohol increased serum cholesterol, triglyceride concentration and decreased HDL-cholesterol concentration after 5 weeks. Serum apolipoprotein B concentration increased after 3 weeks and LDL-cholesterol and apolipoprotein A concentration changed after 1 week. Liver cholesterol and triglyceride concentration increased after 3 weeks. Consumption of high cholesterol diet changed liver and serum lipid composition after 3 weeks. Swiching to normal diet for 5 weeks did not normalize most of lipid composition in serum and liver except serum and liver except serum cholesterol, triglyceride and liver cholesterol. Liver cytosol FABP content and the fatty acid binding capacity decreased dramatically after 1 week with alcohol consumption. This results indicate that FABP content changes before the changes before the changes of blood or liver lipid composition, suggesting changes of FABP may cause development of the fatty liver induced by alcohol and can be used as an index of detecting a early development of fatty liver.

• Journal of Nutrition and Health
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• v.18 no.3
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• pp.234-241
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• 1985

The purpose of this study was to investigate changes in nutritive quality of fermented wheat bran prepared by culturing with a microorganism, Aspergillus oryzae, in an attempt to improve the quality of protein in feedstuff. After incubation of wheat bran with Aspergillus oryzae, the contents of chemical composition, including amino acids, riboflavin and amino-nitrogen were increased, but the level of nitrogen free extract was reduced. The effects of supplementation of fermented wheat bran on the rat diets were evaluated by measuring growth rate, feed efficiency and biological values, such as NPU, PER and NPR. Sixty four male Sprague Dawley rats of 5-6 weeks of age were adopted for the feeding trial for 10 days period, and levels of dietary protein were set at 10%. The %contribution of protein from casein, wheat bran and fermented wheat bran for the other dietary treatments were, in the order, 50-50-0%, 50-30-20%, 50-20-30% and 50-0-50%, respectively. In the result of this study, no significant difference were observed in the amount of feed intake body weight gain and feed efficiency. On the whole, the values of NPU, PER and NPR of all the fermented wheat bran groups appeared to be higher than those of the group whose half of the protein was from wheat bran.