• Title/Summary/Keyword: Feeding Trial

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Safety Test of Brown Rice Expressing Arabidopsis Calcium Transporter by Feeding Trial in Mice (애기장대 칼슘수송체를 발현하는 형질전환 현미의 생쥐 식이를 통한 안전성 평가)

  • Kim, Kyung-Min;Kim, Chang-Kil;Kim, Byung-Oh
    • Journal of Life Science
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    • v.18 no.10
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    • pp.1390-1394
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    • 2008
  • Previously, we found that the transgenic rice plants over-expressing the Arabidopsis $H^+/Ca^{2+}$ antiporter CAX 1 (accession no. U57411) gene accumulated 2.7 to 7.5-fold more calcium in the T3 rice grains as compared to those of control. To examine physiological safety of the $T_3$ rice grains, the effect of the $T_3$ brown rice on change in levels of body weight and white blood cells was compared with that of the control Ilpum brown rice by feeding trial in mice. During the feeding trial for one month, there was no significant difference between two mice groups, which were fed by the $T_3$ brown rice or Ilpum brown rice. There were no detectable differences in their effects on immune functions including plaque-forming unit, peritoneal macrophage number, and NK-cell activity. In addition, biochemical analysis of the blood failed to exhibit any difference between two mice groups. Together, these results suggested that the $T_3$ brown rice, which was produced from a genetically modified organism (GMO), might be safe and possess a potential to be applicable as calcium-fortified feed or food. Long-term safety of the $T_3$ brown rice, however, remains to be elucidated.

Effect of Dietary β-Mannanase Supplementation and Palm Kernel Meal Inclusion on Laying Performance and Egg Quality in 73 Weeks Old Hens

  • Lee, Jun Yeob;Kim, Sang Yun;Lee, Jae Hwan;Lee, Jeong Heon;Ohh, Sang Jip
    • Journal of Animal Science and Technology
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    • v.55 no.2
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    • pp.115-122
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    • 2013
  • This study was conducted to evaluate the effect of dietary ${\beta}$-mannanase supplementation and palm kernel meal (PKM) inclusion (5%) on laying performance, egg quality and nutrient utilizability of laying hens with 73 weeks of age. A total of 240 Lohmann brown laying hens with average 77.5% egg production were randomly allocated with 60 hens per treatment, 4 replicates per treatment and 15 hens per replicate. Experimental design was a completely randomized design with $2{\times}2$ factorial arrangement, with the factors being (1) two levels of PKM (0 vs. 5%) and (2) with or without dietary ${\beta}$-mannanase (480 IU/kg of diet CTCzyme$^{(R)}$) supplementation. All hens were housed in cages ($35cmW{\times}35cmD{\times}40cmH$) with 2 hens per cage for six weeks feeding trial. Laying performance was recorded daily during feeding trial. Egg quality, nutrients utilizability and blood assays were done at the end of feeding trial. Egg production was improved (P<0.05) by both dietary PKM inclusion and ${\beta}$-mannanase combined supplementation. Either ${\beta}$-mannanase or PKM did not affect feed intakes and feed conversion ratio of all diets. Egg weight of hens fed diet containing 5% of PKM had heavier (P<0.05) eggs compared with hens fed without PKM. Albumen height was improved (P<0.05) by dietary mannanase supplementation. Crude fat utilization of 5% PKM diet was higher than that of no PKM diet regardless of ${\beta}$-mannanase supplementation. Both DM and total carbohydrate utilization were decreased (P<0.05) in hens fed 5% PKM diet. Serum IgG and yolk IgY contents of PKM groups were lower (P<0.05) than those of no PKM groups. This result showed that 5% PKM diet, independent of dietary ${\beta}$-mannanase supplementation, was able to improve egg production. In addition, dietary ${\beta}$-mannanase supplementation could be used for improving the albumen height of eggs.

Changes in chemical Components of Muscle from Red Sea Bream (Pagrus major)by Ulva pertusa Extract (구멍갈파래 추출물에 의한 참돔 근육의 화학성분 변화)

  • 배태진;강동수;최옥수;이영재;김경은;김현주
    • Journal of Life Science
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    • v.10 no.5
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    • pp.447-455
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    • 2000
  • A eight weeks feeding experiment was conducted to investigate the effects of different dietary dimethyl-$\beta$-propiothetin (DMPT) levels on growth and chemical components in red sea bream (Pagrus major). The content of DMPT extracted from Ulva pertusa by auroclaving was 32.5mg%. Body weight gain, feed efficiency and feed intake were significantly increased with dietary DMPT level. After eight weeks of feeding trial, moisture content decreased with supplemented DMPT level, while crude protein and lipid contents increased with dietary DMPT level. Amino acid contents of muscle was elevated in the DMPT added groups compared to control group. Amino acid composition of muscle in fish fed the diets supplemented DMPT was not different to that of control group. Fatty acid contents of muscle in fish increased with dietary DMPT level.

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Comparison of Sorghum-Sudangrass Hybrids for Feeding Value and Forage Production in Chonnam Rerion (전남지역에 있어서 수수 - 수단그라스 잡종의 사초생산성 및 사료성분 비교)

  • 전우복;최기춘;김광현
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.15 no.1
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    • pp.67-72
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    • 1995
  • To select the highest yield soiling type forage, the yield performance of selected sorghum-sudangrass hybrids imported in 1992 have been evaluated at Chonnam region. The soiling type hybrids used in this experiment were TE-haygrazer, FP-5 Sudan, Sweet sioux V, and X -78050. Three different cuttings were done on July 6, August 6 and September 16 in 1992. This trial was arranged as a randomized complete block design with three replications under taken over a period 5 months from May to September in 1992. The results of this experiment were summarized as follows: The hybrids tested were not significantly different by plant height, leaf blade length, leaf blade width and stem diameter. In the first cutting, dry matter yield of FP-5 Sudan was the highest. In the second and third cutting, dry matter yield of Sweet sioux V was the highest among hybrids. The contents of crude protein, hemicellulose, relative feeding value(RFV), and amino acid were the highest by the first cutting, but NDF and ADF were shown the lowest.

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Effect of Dietary Root Powder of Angelicae gigantis on Growth Performance, Organ Weight and Serum Components in Broiler Chicken (당귀근부 첨가사료가 육계의 생산성과 장기발육 및 혈액성장에 미치는 영향)

  • 조성구
    • Korean Journal of Poultry Science
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    • v.22 no.3
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    • pp.145-153
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    • 1995
  • The root powder of Angelicae gigantis Radix (RPAGR) is known to he beneficial for liver and blood circulation. In a 6-wk broiler feeding trial, the RPAGR was added at levels of 0, 0.2, 0.5, and 1.0%, respectively. The RPAGR was found to be palatable as birds consumed moreof the test diets than the control(0% RPAGR) diet. The BW gain of 1.0% RPAGR group was higher than the control group. The feed efficiency(F/G) was improved significantly(P<0.01) by feeding 1.0% RPAGR. The RPAGR also caused beneficial effects in various carcass characteristics, i.e., carcass weight, dressing percentage, breast muscle, and thigh meat production. There was no consistent trend in various organ weights by the addition of RPAGR. Also, no consistent trend was found in abdominal fat weight. The serum cholesterol level tended to decrease as the BW increased. No consistent trend was detected in serum triglyceride contents.

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Changes in Chemical Components of Muscle from Korean Rockfish (Sebastes schlegeli) by Enteromorpha intestinalis Extract (창자파래 추출물에 의한 조피볼락 근육의 화학성분 변화)

  • 배태진;강동수;최옥수;이영재;김경은;김현주
    • Journal of Life Science
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    • v.10 no.6
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    • pp.548-557
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    • 2000
  • A eight weeks feeding experiment was conducted to investigate the effects of different dietary dimethyl-$\beta$-propiothetin (DMPT) levels on growth and chemical components in Korean rockfish (Sebastes schlegeli). The content of DMPT extracted from Enteromorpha intestinalis by autoclaving was 31.1 mg%. Body weight gain and feed efficiency were significantly increased with dietary DMPT level. After eight weeks of feeding trial, moisture content decreased with supplemented DMPT level, while crude protein and lipid contents increased with dietary DMPT level. Amino acid contents of muscle was elevated in the DMPT added groups compared to control group. Amino acid composition of muscle in fish fed the diets supplemented DMPT was not different to that of control group. Fatty acid contents of muscle in fish increased with dietary DMPT level.

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The Effects of Caponization Age on Muscle Characteristics in Male Chicken

  • Chen, Kuo-Lung;Chen, Tsai-Tzu;Lin, Kou-Joong;Chiou, Peter Wen-Shyg
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.11
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    • pp.1684-1688
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    • 2007
  • This study examined the caponization effects on the muscle characteristics (quality and quantity) of caponized male chickens fed before or after sexual maturity. Healthy and uniform Single Comb White Leghorn chickens were caponized at 3-week-old. Feeding was conducted until 16-week-old in trial 1 or from 12-week-old to 26-week-old in trial 2. Ten sham operated male chickens (Sham) were also assigned to each trial as the control group. Chickens used in both trials were housed in individual cages with each chicken representing one replicate. The results showed that early caponization (3-week-old) significantly increased (p<0.05) body weight and pectoral major muscle weight and percentage at 16-week-old compared to the Sham in trial 1. Caponization significantly increased (p<0.05) the protein content of the pectoral major muscle, but decreased (p<0.05) the ash content. Late caponization (12-week-old) significantly decreased (p<0.05) the ash content, myofibrillar ATPase activity and emulsification capacity of the pectoral major muscle in mature capons (26-week-old) compared to the Sham in trial 2. Early caponization (3-week-old) only increased the weight and protein content of the pectoral major muscle with decreased ash content in 16-week-old capons. Late caponization (12-week-old) showed no affects on pectoral major muscle quantity, while it decreased the ATPase activity and enhanced the emulsification capacity in mature (26-week-old) capons. Hence, the muscle quality improvement was shown as capons were fed to sexual maturity.

EFFECT OF SUPPLEMENTATION AND PARASITIC INFECTION ON PRODUCTIVITY OF THAI NATIVE AND CROSS-BRED FEMALE WEANER GOATS I. GROWTH, PARASITE INFESTATION AND BLOOD CONSTITUENTS

  • Pralomkarn, W.;Kochapakdee, S.;Choldumrongkul, S.;Saithanoo, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.7 no.4
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    • pp.547-554
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    • 1994
  • This paper presents the effects of supplementary feeding and internal parasites on the growth rates of female weaner goats raised under improved management. A completely randomized $3{\times}3{\times}2$ factorial design was used. Factors were genotype (Thai native: TN, 75% TN $\times$ 25% Anglo-Nubian: An and 50% TN $\times$ 50% AN), feeding grazing only, low (1.0% BW/d) and high (1.5% BW/d) supplementation and parasite control (undrenched and drenched). It was shown that native goats had significantly (p<0.05) higher growth rates than did the cross-bred goats from 12-24 weeks of the trial. The growth rate of goats grazing improved pasture depended on the amount of concentrate offered as a supplement. There was no significant difference in growth rates between undrenched and drenched goats. There was no interaction effect on growth rates between the treatments. Drenched goats had significantly (p<0.01) lower egg counts per gram of gastro-intestinal nematode than did undrenched goats. There was no significant difference between the treatments for blood constituents (total protein, haemoglobin, packed cell volume, eosinophils, lymphocytes, monocytes and basophils).

FEEDING OF BYPASS PROTEIN TO CROSS BRED COWS IN INDIA ON STRAW BASED RATION

  • Kunju, P.J.G.;Mehta, A.K.;Garg, M.R.
    • Asian-Australasian Journal of Animal Sciences
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    • v.5 no.1
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    • pp.107-112
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    • 1992
  • Feeding of bypass protein to lactating animals have been suggested by many research scientists as a way to increase the nutrient supply at the intestinal level thereby enhance animal production in ruminant animals. A feeding trial with a formulated bypass protein feed on straw based ration was carried out by using lactating cross bred cows at the stage of 4th month of their lactation. Bypass protein feed was fed at 5 different levels. Urea Molasses Block was used as a nitrogen source to the rumen microflora. In order to reduce the heat increment straw intake was restricted to all the animals. Urea Molasses Block intake was noticed varying in proportion with the bypass protein feed intake. Milk production was observed increasing in accordance with the level of bypass protein feed intake. However, the maximum response was noticed in cows that were fed 3 kg bypass protein feed. The nutrient availability at this stage was below the NRC (1988) requirements. Other remarkable finding was that the cows maintained the persistency of milk production even after 3rd month of lactation when the ambient temperature was $40^{\circ}C$.

Effects of Feeding Various Types of Fermented Red Ginseng Marc with Red Koji to Laying Hens on Eggshell and Egg Yolk Quality (여러 제형의 홍국발효 홍삼박을 산란계 사료에 첨가시 난각과 난황 특성에 미치는 영향)

  • Chung, Tae-Ho;Choi, In-Hag
    • Journal of Environmental Science International
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    • v.26 no.7
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    • pp.869-874
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    • 2017
  • This study was conducted to investigate the effects of dietary supplementation of various types of fermented red ginseng marc with red koji to laying hens on eggshell and egg yolk quality characteristics. A total of 240 Hy-line Brown laying hens (40 wk of age) were randomly allotted to 24 pens (6 replicates per treatment and 10 laying hens per replicate). Experimental diets consisted of 4 treatments containing basal diet (control), 1% fermented red ginseng marc powder with red koji, 1% fermented red ginseng marc pelleted with red koji and 1% fermented red ginseng marc coated with red koji. During the 8-wk feeding trial, there were no significant differences in eggshell strength, eggshell thickness and eggshell color among the treatments, except for eggshell strength at 4 and 8 wk and eggshell thickness at 0 wk. In addition, no differences in egg yolk color and egg yolk index were found for all treatments throughout the 8-wk feeding period, except for egg yolk color at 0 wk. Thus, using various types of fermented red ginseng marc with red koji to laying hens did not improve eggshell and egg yolk quality characteristics.