• ALGAE
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• v.26 no.1
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• pp.87-96
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• 2011

Protein profiles of a common mixotrophic dinoflagellate, Prorocentrum micans, growing autotrophically and mixotrophically (fed on the cryptophyte Rhodomonas salina) were compared using two-dimensional gel electrophoresis (2-DE) to determine if they vary in different trophic modes. Approximately 2.3% of the detected proteins were differentially expressed in the different trophic modes. Twelve proteins observed only in the mixotrophic condition had lower pI value (<5) than the fifteen proteins observed only in the autotrophic condition (>5). When the internal amino acid sequences of five selected proteins differentially expressed between autotrophic and mixotrophic conditions were analyzed using matrix-assisted laser desorption time-of-flight (MALDI-TOF) mass spectrometry, two proteins that were specifically expressed in the autotrophic condition showed homology to glyceraldehyde-3-phosphatase dehydrogenase (GAPDH) and a bacterial catalase. Three mixotrophy-specific proteins showed homology to certain hypothetical proteins from an insect and bacteria. These results suggested the presence of certain gene groups that are switched on and off according to the trophic mode of P. micans.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.27 no.6
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• pp.31-38
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• 2013

A pulse driven atmospheric plasma jet controlled by external ballast capacitor is developed. Unlike the most commonly use DBD sources, the proposed device utilizes bare metal electrode. The discharge energy per pulse can precisely be determined by changing voltage and capacitance of the ballast capacitor. It is shown that the device can provide wide range of plasma, from stable glow mode to near arc state. Current-voltage waveforms, optical emission spectra and discharge images are investigated as a function of an injection energy. The OES shows that He and oxygen lines are increased as a function of the external ballast capacitor. Ozone and rotational temperature have similar tendency with a power consumption. The feeding gas is He and the applied DC voltage is from 400V to 800V when the gap distance is $500{\mu}m$.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.287-290
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• 2000

To examine effects of agitation and aeration as well as adding of glucose and yeast extract on cell growth and polysaccharide production by Paecilomyces japonica, batch culture was carried out at 5L jar fermenter at $27^{\circ}C$ with the initial pH 7 for 7 days cultivation(innoculum size 2%, working volume 3L). Media compositions(g/L) were 30 glucose, 20 yeast extract, 0.5 $KH_2PO_4$, $0.1\;CuCl_2\;{\cdot}\;2H_2O$. Optimum culture conditions of agitation and aeration in batch culture were 400 rpm and 1.0 vvm, resulting in 23.1 g/L biomass and 2.5 g/L polysaccharide. Additional feeding of glucose and yeast extract with a pulse mode conferred an advantage on cell growth and polysaccharide production with showing the results of 29.2 g/L and 3.3 g/L, respectively.

• KSBB Journal
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• v.11 no.5
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• pp.524-528
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• 1996

Bacterial degradation of 4-chloro-2-methylphenoxyacetic acid (MCPA) was studied in column reactors under conditions approximating a fluidized bed system, with granular activated carbon (GAC) as a support matrix. A mixed bacterial culture of MCPA-degrading bacteria was used as an inoculum to develop a biofilm on GAC. Initially, adsorption of MCPA by GAC and blofilm formation on GAC were examined. MCPA degradation was evaluated with a batch and continuous mode of operation of the GAC fixed-film column reactors. In the batch operations, complete degradation of MCPA was achieved during the incubation period. Partial degradation of MCPA occurred in the continuous operations and MCPA degradation was dependent on the feeding rate of MCPA solution.

• Transactions of the Korean Society of Mechanical Engineers
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• v.19 no.10
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• pp.2678-2689
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• 1995

In this study, a new hot-wire anemometer which has greater sensitivity than that of a constant temperature anemometer (CTA) was proposed. In contrast to CTA, the wire working resistance of the new anemometer increases with flow velocity, that is, the operating mode of the wire becomes variable temperature. The variable temperature anemometer(VTA) was made by substituting a voltage controlled variable resistor such as photoconductive cell or transistor for one of the resistors in the bridge. By positively feeding back the bridge top signal to the input side of these electronic components, the wire overheat ratio could be increased with velocity automatically. Static response analyses of the VTA, constant voltage anemometer (CVA) and CTA were made in detail and calibration experiments were performed to validate the proposed operating principle. The wire operating resistance of the CVA decreases with velocity and this leads to lower sensitivity than that of a CTA. But the sensitivity of the newly proposed VTA is superior to that of a CTA, since the wire overheat ratio increases with velocity. Consequently, it is found that the major factor that is responsible for large sensitivity of a VTA is not the working resistance itself but the change of the wire working resistance with velocity.

• Transactions of Materials Processing
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• v.17 no.1
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• pp.52-58
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• 2008

Tube hydroforming process is generally consisted with pre-bending, preforming and hydroforming processes. Among forming defects which may occur in tube hydroforming such as buckling, wrinkling and bursting, the wrinkling and bursting by local instability under excessive tensile stress mode were mainly caused by thinning phenomenon in the manufacturing process. Thus the accurate prediction and suitable evaluation of the thinning phenomenon play an important role in designing and producing the successfully hydroformed parts without any failures. In this work, the formability on hydroformed part for automobile, i.e. engine cradle, was evaluated using finite element analysis. The initial tube radius, loading path with axial feeding force and internal pressure, and preformed configuration after preforming process were considered as the dominant process parameters in total tube hydroforming process. The effects on these process parameters could be confirmed through the numerical experiments with respect to several kinds of finite element simulation conditions. The degree of enhancement on formability with each process parameters such as initial tube radius, loading path and preform configuration were also compared. Therefore, it is noted that the evaluation approach of the formability on hydroformed parts for lots of industrial fields proposed in this study will provide one of feasible methods to satisfy the increasing practical demands for the improvement of the formability in tube hydroforming processes.

• Journal of Korea Foundry Society
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• v.23 no.5
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• pp.244-250
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• 2003

The aim of this study is fabricating of composite filler metal (CFM) by a combination of selective laser sintering (SLS) of stainless steel powders (RapidSteel $2.0^{TM}$ and liquid phase infiltration of Ag-28 wt.%Cu alloy. Porous stainless steel body with inter-connected pore channels was fabricated by SLS, binder decomposing and densification processes. By the direct contact infiltration, the narrow inter-particle channels of the porous body were completely filled with the Ag-28 wt.%Cu alloy infiltrant. During infiltration, the dissolved elements of Fe, Ni and Cr from the porous body were solved into copper solid solution phases, which consist of eutectic structure of composite metal matrix. The S10C/CFM/S10C joints, which have narrow clearance gaps between them up to 10 micrometers, were joined successfully by self-feeding of filler metal from the matrix of CFM. The CFM kept its original thickness and microstructure after brazing. The tensile strength of brazed specimen was higher than 30 kgf/$mm^2$ and showed a typical ductile fracture mode in the CFM.

• Journal of electromagnetic engineering and science
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• v.13 no.2
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• pp.93-103
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• 2013

This paper presented a novel approach for the design of a tunable dual-band bandpass filter (BPF) with independently tunable passband center frequencies and bandwidths. The newly proposed dual-band filter principally comprised two dual-mode single band filters using common input/output lines. Each single BPF was realized using a varactor-loaded transmission line resonator. To suppress the harmonics over a broad bandwidth, a defected ground structure was used at the input/output feeding lines. From the experimental results, it was found that the proposed filter exhibited the first passband center frequency tunable range from 1.48 to 1.8 GHz with a 3-dB fractional bandwidth (FBW) variation from 5.76% to 8.55%, while the second passband center's frequency tunable range was 2.40 to 2.88 GHz with a 3-dB FBW variation from 8.28% to 12.42%. The measured results of the proposed filters showed a rejection level of 19 dB up to more than 10 times the highest center frequency of the first passband.

• Microbiology and Biotechnology Letters
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• v.28 no.5
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• pp.291-297
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• 2000

A strain producing high levels of compaction was isolated from soil and identified as Penicillium sp. Y-8515 based on the morphological characteristics and ribosomal RNA sequence analysis. Optimization of several different carbon and nitrogen sources for the effective production of compaction was performed resulting in the medium compositions containing 5%(w/v) glucose, 1.0 % soybean meal, 0.5% yeast extract, 0.5%(NH$_4$)$_2$$SO_4$, 0.25%,$ NaH_2$$PO_4$, 0.25% $CaCO_3$. The fixed con-centration of glucose(5%, w/v) and relatively lower concentrations(less than 2.5%, w/v) of soybean meal stimu-lated the transformation of the growth morphology from filamentous to pellet form. Comparing to that by filamentous form, the production of compactin by pellet form increased up to 1.5 folds. In a fed-batch fermentation, continuous feeding of the mixture of glucose and nitrogen source at the ratio of 10:1 showed 3.5-fold more produc-tion yield of compaction comparing to the batch mode.

• Membrane Journal
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• v.7 no.4
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• pp.175-182
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• 1997

Membrane-bioreactor process which combines anaerobic-aerobic bioreactor and membrane process, was used to remove refractory organic susbstances and dye molecule in textile wastewater effectively. Direct feeding of raw feed water to membrane process caused serious fouling on membrane. On the other hand, pretreated feed by bioreactor before the membrane process remarkably reduced the fouling and prolonged the membrane life. Removal efficiency and fouling were more dependent on the material property of the membrane rather than the membrane pore size and structure. Operation mode of hollow fiber membrane module and linear velocity in the hollow fiber influenced the ramoval efficiency and the water flux of the membrane. The combined membrane- bioreactor process was more effective in treating the textile wastewater than each single process.