• Molecules and Cells
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• 제37권6호
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• pp.473-479
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• 2014

Spermatogonial stem cells (SSCs, also called germline stem cells) are self-renewing unipotent stem cells that produce differentiating germ cells in the testis. SSCs can be isolated from the testis and cultured in vitro for long-term periods in the presence of feeder cells (often mouse embryonic fibroblasts). However, the maintenance of SSC feeder culture systems is tedious because preparation of feeder cells is needed at each subculture. In this study, we developed a Matrigel-based feeder-free culture system for long-term propagation of SSCs. Although several in vitro SSC culture systems without feeder cells have been previously described, our Matrigel-based feeder-free culture system is time- and cost-effective, and preserves self-renewability of SSCs. In addition, the growth rate of SSCs cultured using our newly developed system is equivalent to that in feeder cultures. We confirmed that the feeder-free cultured SSCs expressed germ cell markers both at the mRNA and protein levels. Furthermore, the functionality of feeder-free cultured SSCs was confirmed by their transplantation into germ cell-depleted mice. These results suggest that our newly developed feeder-free culture system provides a simple approach to maintaining SSCs in vitro and studying the basic biology of SSCs, including determination of their fate.

• 한국생산제조학회지
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• 제19권3호
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• pp.353-358
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• 2010

This research introduces a new and improved design for a pneumatic cylinder driven roll feeder wherein each of the principal rotating feeder parts is configured so as to have feeding accuracy and to be low manufacturing cost. The feed pitch accuracy of the proposed roll feeder was evaluated by measuring lengths of cut offs of the strip stock with a shear attached to an air press. The air press was designed, manufactured, and mounted on the same table of the proposed roll feeder such that the strip stock maintained horizontal plane until the strip stock entered into the shear. The proposed roll feeder and the air press were designed to be operated automatically by a PLC employed controller. The feed pitch accuracy of the proposed roll feeder was analyzed by setting the pitch as 10, 12.5, and 15mm. At each predetermined feed pitch, the proposed roll feeder was tested 300 times as one test set and replicated three times. The average lengths of the cut offs of the strip stock ranged from 9.98 to 10.13mm, from 12.42 to 12.57mm, and from 14.96 to 15.06mm at the predetermined 10, 12.5, and 15mm feed pitch, respectively, among the total of 900 samples of each feed pitch. Main cause of variation of the length of the cut off of the strip stock fed by the proposed roll feeder was considered to be fluctuation of the air press during recompressing period of the air compressor to pressurize the air in the air tank. The largest difference between the maximum and the minimum length of the cut off was appeared while the air compressor recompressing the air. The air compressor used for this study restricted the air delivered to the proposed roll feeder while it was still running. Thus, this air delivery restriction problem should be improved by stabilizing the air press while the proposed roll feeder is running.

• 대한전기학회논문지:전력기술부문A
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• 제51권5호
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• pp.238-246
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• 2002

Utilities are trying to install the equipment of high quality to avoid deterioration of supply reliability. In addition, many sectionalizing switches which can decrease the total outage value for a fault are installed for the same reason. Therefore, utilities are interested in stun dards and criteria for installing switches to optimize the total cost on distribution systems. The affect of sectionalizing switches installed on distribution feeder is gradually decreased because the failure rate on distribution feeder is decreased. Also the automation for distribution systems is widely applied for the efficient operation. Therefore, the renewal for installation standards of sectionalizing switches Is required to reflect the current operation situation. The variable data is used to consider the KEPCO's real situation of distribution feeder as follows; the feeder capacity, connecting rate, feeder length, failure rate of distribution feeder, the failure rate of switches, perception time of feeder fault, the restoration time for a faulted section, the transfer time to other feeders, and the switching time. In this study, We propose equations which can determine the number of sectionalizing switches for minimizing the outage and switch installation cost.

• 한국항해항만학회지
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• 제35권7호
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• pp.605-611
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• 2011

In this paper, practical problems for current feeder service between Busan port and west coast of Japan were extracted through in depth interviews with Japanese feeder vessel companies, shippers, and port authority based upon which major criteria (elements) for improvement of the feeder network are structured in hierarchic order and weighed relative importance through AHP method. From the questionnaire answered by Korean and Japanese port users and experts the weights of criteria were calculated and the shipping service was ranked the first by both parties with 0.235 and 0.217, respectively. The port service and support system ranked the second and the third, whereas the port marketing was shown to be the lowest of all. Considering the overall weights, the increase in a port loading and unloading speed of port service was followed by provision of dedicated deck for feeder vessel of port facilities for Korean respondents. Therefore, speed up of the port operations and providing docks for feeder vessel are to be firstly provided for more efficient feeder operations, which would be the basis for the construction of optimal transportation network.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2002년도 하계학술대회 논문집 A
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• pp.70-73
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• 2002

Utilities are trying to install the equipment of high quality to avoid deterioration of supply reliability. In addition, many sectionalizing switches which can decrease the total outage value for a fault are installed for the same reason. Therefore, utilities are interested in standards and criteria for installing switches to optimize the total cost on distribution systems. The affect of sectionalizing switches installed on distribution feeder is gradually decreased because the failure rate on distribution feeder is decreased. Also the automation for distribution systems is widely applied for the efficient operation. Therefore, the renewal for installation standards of sectionalizing switches is required to reflect the current operation situation. The variable data is used to consider the KEPCO's real situation of distribution feeder as follows; the feeder capacity, connecting rate, feeder length, failure rate of distribution feeder, the failure rate of switches, perception time of feeder fault, the restoration time for a faulted section, the transfer time to other feeders, and the switching time. In this study, We propose equations which can determine the number of sectionalizing switches for minimizing the outage and switch installation cost.

• 한국압력기기공학회 논문집
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• 제17권1호
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• pp.28-35
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• 2021

The flow accelerated corrosion (FAC) is one of significant aging and degradation mechanism and can affect structural integrity of CANDU feeder pipes. Pipe burst can occur under normal operation pressure (min. 10 MPa) if wall-thinning of the feeder pipe due to FAC is accumulated. Previous studies considered simple shapes of feeder pipe with local wall-thinning in order to conservatively assess structural integrity of wall-thinned feeder pipe. In this paper, a new FE model is developed, having an actual shape of the feeder pipe (double bent) as well as the actual wall-thinning shape and location based on the in-service inspection result. Then, the burst pressure assessment of the wall-thinned feeder pipe is performed using lower bound limit load analysis considering elastic-perfectly plastic material. In addition, an improved formulation to predict the burst pressure of the wall-thinned feeder pipe is presented and the safety margin is compared with an existing assessment method.

• Transactions on Electrical and Electronic Materials
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• 제7권5호
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• pp.247-251
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• 2006

The traction DC feeder cable is one of the key devices for the safety operation of subway system, but for low voltage DC feeder cable (<3000 V) for subway, little attention has been paid by investigators on its online monitoring technology. With an introduction of cable laying and operation environment for the cable, this paper investigated the on-line monitoring technology of 1500 V DC feeder cable of subway. Firstly, in the text, the fault model of 1500 V DC cable was proposed based on the analysis of the fault type of the DC feeder cable, and then put forward synthetically on-line monitoring discharge signal and DC leakage current signal to assess DC feeder cable insulating state. The results of laboratory experiment prove that the proposed methods are feasible and can be implemented on-line monitor on DC feeder cable of subway.

• Journal of Biosystems Engineering
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• 제35권3호
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• pp.197-205
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• 2010

An automatic echelon feeder of brown rice was presented for quality inspection system using color image processing. A echelon feeder was developed with vibratory feeder and cylindrical indent singulator having oblique light. The vibratory feeder consisted of a hopper, electromagnetic vibrator and multichannel grooves and supply the grain sample to the singulator. The feeding performance such as feed rate, blocking frequency of the channel was dependent on the size of groove and vibration pattern. A cylindrical indent singulator consisted of a rotating cylinder, prisms and a tungsten-halogen light source. It delivered grain kernels under the camera in a echelon form and illuminate the kernels with oblique ray and ambient light. The size of the indents installed on the surface of the rotating cylinder was determined by the dimensions of the paddy and a small triangular prism was placed in each indent to apply $ 20^{\circ}$ oblique light to the grain kernel.

• 한국산업융합학회 논문집
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• 제16권4호
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• pp.133-140
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• 2013

This paper presents an overview of the concepts of optimal feeder design in relation to the loading of bulk solids for Automatic filling system that should be control the amount of goods and packaging to seal. Feeder modular device, important parts of the package, so in order to perform a conceptual design optimization techniques are applied in two steps. First of all derive the problems through structural analysis for the conceptual model of vibrating feeder. Secondly derive reasonable design model based on the results of the structural analysis of modified boundary shape and then verify it. The proposed system has the following goal that is satisfies the dynamic stability with minimum weight and optimization of the shape. As a result, the weight reduction of feeder is 2.1% and 7% increase in the natural frequency.

• Reproductive and Developmental Biology
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• 제36권4호
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• pp.291-294
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• 2012

Embryonic stem cell classically cultured on feeder layer with FBS contained ES medium. Feeder-free mouse ES cell culture systems are essential to avoid the possible contamination of nonES cells. First we determined the difference between ES cell and MEF by Oct4 population. We demonstrate to culture and to induce differentiation on feeder free condition using a commercially available mouse ES cell lines.