• Asian-Australasian Journal of Animal Sciences
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• v.14 no.4
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• pp.540-547
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• 2001

An experiment was conducted to investigate the effects of environmentally friendly agents (yucca extract, mineral feed additive, acidifier, nonspecific immunostimulating anionic alkali solution) on the growth performance, nutrient digestibility, nutrient excretion and carcass characteristics in growing-finishing pigs. A total of 125 pigs were allotted into 5 treatments with 5 replications, 5 pigs per pen in a completely randomized block design. Dietary treatments consisted of 1) control: basal diet, 2) YE: basal diet+120 mg/kg of yucca extract, 3) MFA: basal diet+50 mg/kg of mineral feed additive, 4) NIS: basal diet+3% of NIS (nonspecific immunostimulating anionic alkali solution), and 5) Acidifier: basal diet+0.5% of acidifier (organic acid complex). During the growing phase of the feeding trial, There were no significant differences in average daily feed intake (ADFI) and feed conversion ratio (F/G) among treatments, but the Acidifier group showed higher average daily gain (ADG) than the MFA group (p<0.05). During the finishing phase, there were no significant differences in growth performance among treatments, however YE supplementation increased ADG and F/G by 3.1 and 6.15%, respectively, when compared to control group. Proximate nutrients (dry matter, DM; crude protein, CP; crude ash, CA; crude fat, CF; gross energy, GE; calcium, Ca and phosphorus, P) digestibility did not show any significant differences among the treatments. And there were no significant differences in DM, N and P excretion as well. The supplementation of environmentally friendly agents tended to increase carcass weight compared to control group. Pigs fed MFA showed significantly heavier (p<0.05) carcass weight than those fed control or Acidifier diets. Addition of these agents except for NIS to diet for growing-finishing pigs caused a similar feed cost/kg weight gain than control group. Therefore, present study suggested that the optimum environmentally friendly agent would be yucca extracts, considering the effects on growth performance, nutrient digestibility, nutrient excretion, carcass characteristics and feed cost in growing-finishing pigs.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.12
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• pp.1734-1741
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• 2016

This study was conducted to examine the effects of adding phytoncide oil extracted from Korean pine nut cone by-product to the diet of dairy cows on milk yield and compositions, fatty acid characteristics, complete blood count and stress response. A total of 74 Holstein cows were used for 30 days and divided into two groups. Each group was given a basal diet (C) or an experimental diet containing phytoncide additives at 0.016% (T) in feed. The results showed that phytoncide feeding had no effect on milk yield. In addition, there were no observed effects on milk composition, but the ratio of fatty acid in milk was significantly affected by the phytoncide diet, and it showed a positive effect. Not only were the major functional fatty acids, conjugated linoleic acid and eicosapentaenoic acid increased, but also ${\omega}6:{\omega}3$ fatty acid ratio was reduced in milk of T group (p<0.05). In blood analysis, the complete blood count showed no significant difference between C and T group on all parameters. However, the cortisol concentration was significantly decreased in T group compared to control (p<0.05). Taken together, we suggest that phytoncide oil does not have a great influence on the physiological changes, but can be a potential feed additive that improves the milk fatty acid and stress resilience in dairy cows. In addition, it will contribute to the development of feed resource, a reduction in feed cost and a lessening of environmental pollution.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.10
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• pp.1451-1457
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• 2016

A comparative study was performed to investigate the efficacy of KiFAY as a feed additive on performance parameters, thyroid, and pancreatic hormone levels in broilers. Ninety birds (Vencobb 400) were randomly divided into three groups viz., Control (no DL-methionine supplementation), Treatment1 (containing added DL-methionine) and Treatment 2 (containing KiFAY and without DL-methionine supplementation). The performance parameters (weekly body weight, body weight gain, feed intake, and feed consumption ratio) were recorded and calculated during the whole study of 4 weeks. Analyses of insulin and insulin-like growth factor (IGF 1), triiodothyronine (T3), thyroxine (T4) and thyroid stimulating hormone (TSH) were performed at the end of the study. The results show that birds on supplementation of KiFAY performed significantly (p<0.001) better than other treatments. The weekly body weight, body weight gain, feed in-take and feed consumption ratio improved in KiFAY treated birds. The study found an increase in insulin and IGF1 levels (p<0.001) in KiFAY compared with the other treatments. Serum T3, T4, and TSH levels in the Treatment 2 were higher than other treatments (p<0.001). The KiFAY supplementation was able to improve performance with associated responses at a hormonal level in broilers.

• Journal of Microbiology and Biotechnology
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• v.16 no.8
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• pp.1248-1254
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• 2006

The effect of Spirulina platens is and probiotics as feed additives on the growth of the shrimp Fenneropenaeus chinensis was investigated in comparison with a control. The shrimp were cultured in rearing tanks in a seawater pond for 35 days from September 1, 2004. As regards the water quality, the probiotic treatment (T2, commercial diet and 3% probiotics) produced a lower TDN (total dissolved nitrogen) and TDP (total dissolved phosphorus), making it effective in water quality improvement. Nonetheless, the phytoplankton flora succeeded from diatoms to cyanobacteria, regardless of the feed additives. Treatment T3, including 3% S. platensis, produced the highest mean body weight, which was 39% higher than that for all the other treatments (P<0.05). Accordingly, it was found that the use of Spirulina and probiotics as feed additives increased the shrimp body weight and improved the water quality, respectively.

• Journal of Korean Clinical Health Science
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• v.1 no.1
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• pp.83-89
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• 2013

Purpose. Natural herb has been used for traditional medicine for thousands of years. For this reason, alternative medicine methods affect to domestic animal field. The main purpose of this study was to confirm the regulated proteins after feed additive treatment. Methods. We used total thirty male pigs were used for this experiment. E. coli and Salmonella typhimurium, were used for promote enteritis. Animals were divided into a negative control group, positive control group and test group to determine the effect of an additive mixture on the changes in protein expression in serum. The pigs were given a food supplemented with a natural herbal additive containing immunoglobulin yolksac (IgY) at concentrations 1%. On the 1st week and after eight weeks of feeding, the serums were collected from each group. The changes in the serum proteome as a response to the herbal additive were examined using two-dimensional polyacrylamide gel electrophoresis. Results. A total of 14 differentially expressed protein spots were identified by comparing the protein profiles of the control and additive treated porcine serums. Finally, 7 proteins were detected by MALDI-TOF/MS. Moreover, the proteins detected are involved in a cholesterol control factor, inflammation regulator, Conclusion. These results support of the hypothesis that a natural herbal additive containing IgY can affect the immune regulation system and reduce the pathological process by microbial infections.

• Fisheries and Aquatic Sciences
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• v.26 no.5
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• pp.355-365
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• 2023

The Pacific abalone, Haliotis discus hannai, is a highly valued and industrially important aquaculture species with growing demands of the expanding abalone aquaculture industry. To explore the feasibility of using the brown alga, Sargassum horneri, as a potential substitute for abalone feed, it is important to identify the feed preference and attractant effect of S. horneri on Pacific abalone. Our experiments indicated that the feeding-associated movement of abalone could be detected using a video tracking system under indirect illumination with dim red light. To further analyze the attraction potentials of various test materials, preference analysis was performed using Avicel-coated glass plates with ground powders of various seaweeds (e.g., S. horneri, Saccharina japonica, and Undaria pinnatifida) and commercial abalone feed, together with coffee waste. Heat map analysis indicated greater attraction by the kelp S. japonica than by S. horneri and commercial feed, which showed similar preference levels. Feeding preference based on the area of Avicel eaten by abalone showed a significant preference for U. pinnatifida over S. horneri (feeding area: 68.6 ± 20.1% vs. 37.5 ± 22.4%, p < 0.05). Additionally, the feeding area was significantly greater for plates with S. japonica than for plates with S. horneri (44.0 ± 16.6% vs. 22.6 ± 15.4%, p < 0.05). There was no significant difference in feeding area between commercial feed and S. horneri (31.7 ± 11.6% vs. 31.6 ± 20.2%, p > 0.05). The methanol extracts attracted abalone in the following order: U. pinnatifida > S. horneri > S. japonica > commercial feed > coffee waste. To determine the attractive effects of the components of methanol extracts, mixtures of methanol extracts of commercial feed with increasing amounts of S. horneri were examined. The results showed a significant increase in feeding preference upon addition of S. horneri up to 50% and 75%, suggesting its potential for use as an appetite-enhancing feed additive. This study identified conditions that can be successfully used to monitor the movement of Pacific abalone; the results of preference analysis confirmed that abalone exhibited similar attraction and feeding preference for S. horneri, compared with commercial feed.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.9
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• pp.1248-1254
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• 2012

The effect of silage additives on ensiling characteristics and nutritive value of Napier grass (Pennisetum purpureum) silages was studied. Napier grass silages were made with no additive, fermented juice of epiphytic lactic acid bacteria (FJLB), molasses or cassava meal. The ensiling characteristics were determined by ensiling Napier grass silages in airtight plastic pouches for 2, 4, 7, 14, 21 and 45 d. The effect of Napier grass silages treated with these additives on voluntary feed intake, digestibility, rumen fermentation and microbial rumen fermentation was determined in 4 fistulated cows using $4{\times}4$ Latin square design. The pH value of the treated silages rapidly decreased, and reached to the lowest value within 7 d of the start of fermentation, as compared to the control. Lactic acid content of silages treated with FJLB was stable at 14 d of fermentation and constant until 45 d of ensiling. At 45 d of ensiling, neutral detergent fiber (NDF) and acid detergent fiber (ADF) of silage treated with cassava meal were significantly lower (p<0.05) than the others. In the feeding trial, the intake of silage increased (p<0.05) in the cow fed with the treated silage. Among the treatments, dry matter intake was the lowest in the silage treated with cassava meal. The organic matter, crude protein and NDF digestibility of the silage treated with molasses was higher than the silage without additive and the silage treated with FJLB. The rumen parameters: ruminal pH, ammonia-nitrogen ($NH_3$-N), volatile fatty acid (VFA), blood urea nitrogen (BUN) and bacterial populations were not significantly different among the treatments. In conclusion, these studies confirmed that the applying of molasses improved fermentative quality, feed intake and digestibility of Napier grass.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.9
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• pp.1274-1279
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• 2003

Thirty-six Naeimi ram lambs were equally and randomly allotted to four treatment groups with three replications per treatment to determine the simple and additive effects of monensin and zeranol on growth performance, carcass characteristics and nutrient digestibility. The treatment groups were: basal diet-fed lambs (C), monensin-fed lambs (M) where the basal diet was supplemented with 33 mg monensin per kilogram DM, lambs implanted with 12 mg zeranol (Z), and monensin-fed lambs implanted with zeranol (MZ). Lambs fed monensin-containing diet consumed 10.5% less (p<0.05) DM/100 kg weight and were 8.3% more (p<0.05) efficient in converting feed than lambs fed control diet. Zeranol implanted lambs tended to grow 35.2% (p<0.05) faster, consumed 5.1% more (p<0.05) feed and were (p<0.05) 21.9% more efficient in their feed conversion than control lambs. Responses of lambs to monensin and zeranol implants were not additive. Except for Z treatment, there were no marked differences in all carcass characteristics among the various treatment groups. Z-lambs produced 12.7% heavier (p<0.05) carcasses compared with those from C treatment. Also fat parameters, namely, kidney and pelvic fat (KP), body wall thickness and fat thickness, indicated trends for higher finish in Z treatment lambs (p<0.05) than for those lambs from other treatments. Except for CF and ADF, no significant differences in nutrients digestibility were noticed between various treatments; feeding monensin resulted in 24.5% and 8.5% depressions (p<0.05) in CF and ADF digestibility, respectively in comparison to C treatment. Nitrogen retention as percentage of total N-intakes was averaging 7.5 and 20.2% higher (p<0.05) in lambs implanted with zeranol than those fed the M and C diets, respectively.

• Korean Journal of Poultry Science
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• v.44 no.3
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• pp.151-159
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• 2017

The experiment was carried out on 648 Ross 308 broilers ($40.4{\pm}0.2$ g; 1 d) chickens. It lasted for 28 days to evaluate the effects of phytogenic feed additive (PFA) on growth performance, nutrient digestibility, cecum microbiota, bone minerals contents, meat quality and internal organ weights. The chickens were haphazardly divided into any 1 of 6 dietary treatments (6 replicate pens/treatment; 18 broilers/pen). The experimental diets were: CON, control; T1, CON + 150 ppm PFA; T2, CP 0.5% reduced from basal diet; T3, T2 + 150 ppm PFA; T4, Ca 0.07% reduced + available P (aP) 0.065% reduced from basal diet; T5, T4 + 150 ppm PFA. Overall, broilers fed with T3 diet tended to have higher (P=0.08) body weight gain (BWG), and lower (P=0.05) feed conversion ratio (FCR) than T2 diet. The apparent total tract digestibility (ATTD) of P was lower (P=0.03) in T2 as compared to CON and it was higher (P=0.02) in T3 as compared to T2. There were no differences in meat quality, relative organ weight, and cecum microbiota (P>0.05). The supplementation of PFA showed trends in improvement in ash content in the bone of birds fed T1, T2 and T4 diets compared with CON. Likewise, the supplementation of PFA tended to increase (P=0.07) Ca content in the bone of birds fed T1 diet compared to CON. In conclusion, dietary supplementation with 150 ppm PFA could improve BWG in birds fed protein reduced diet, improve apparent total tract digestibility (ATTD) of P, and the bone Ca contents in broilers.

• Korean Journal of Agricultural Science
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• v.46 no.1
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• pp.11-25
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• 2019

This study was conducted to evaluate the effects of a composite feed additive (CFA) consisting of vitamin C, vitamin E, selenium, and choline on growth performance, carcass characteristics, and meat properties of Hanwoo steers. Twenty-two late fattening steers were randomly assigned to one of two dietary groups, control (commercial concentrate + rice straw) and treatment (commercial concentrate + rice straw + 50 g of CFA). Average daily gain (ADG) was 4.0% higher in treatment than that in control; however, there was no statistically significant difference. The supplementation of CFA had no effect on overall yield or quality traits of carcass. CFA had no effect on chemical composition, water holding capacity, shear force, cooking loss, or myoglobin values in longissimus muscle; however, the content of ${\alpha}$-tocopherol was increased by supplementation of CFA (p < 0.01). The contents of ${\alpha}$-linolenic acid and n-3 fatty acids were higher in treatment group than in control (p < 0.01). The ratio of n-6/n-3 fatty acids was decreased by supplementation of CFA (p < 0.01). The supplementation of CFA had no effect on pH or thiobarbituric acid reactive substances (TBARS) values in longissimus muscle (raw); however, TBARS (cooked) values were lower in treatment group than in control during refrigerated storage (p < 0.01). Thus, the results indicate that the supplementation of CFA (vitamin C + vitamin E + selenium + choline) had some positive effects on the increase in ${\alpha}$-tocopherol and n-3 fatty in longissimus muscle, without any negative effect on growth performance or carcass characteristics of late fattening Hanwoo steers.