• Animal Bioscience
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• v.36 no.4
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• pp.619-628
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• 2023

Objective: This study aimed to determine and compare the apparent ileal digestibility (AID) and the standardized ileal digestibility (SID) of amino acids (AA) in soybean meal (SBM), cottonseed meal (CSM), and low-gossypol cottonseed meal (LCSM) fed to broiler chickens and laying hens. Methods: Three semi-purified diets containing the identical crude protein concentration at 20% were formulated to contain SBM, CSM, or LCSM as the sole source of N. A N-free diet was also formulated to estimate the basal ileal endogenous losses of AA for broilers and hens. A total of 300 male Ross 308 chicks at one-day-old and 144 Hy-Line Brown laying hens at 30-week-old with initial egg production rate of 88.3%±1.0% were randomly allocated into 1 of 4 dietary treatments, respectively. Results: CSM and LCSM showed more Arg and Cys+Met while less Lys, Ile, Leu, and Thr relative to SBM. Significant interactions existed between species and experimental diets for AID (except for Arg, Asp, Glu, Gly, and Pro) and SID (except for Arg, His, and Phe) of most AA. Most AA in diets showed higher AID (except for Lys) and SID (except for Lys, Met, and Ser) in broilers relative to laying hens. The AID and SID of all AA were significantly different between the three diets. In broilers, the AID and SID of most indispensable AA except for Arg in SBM and LCSM was higher than CSM. In laying hens, the AID and SID of most indispensable AA except for Arg, Met+Cys, and Phe in SBM was higher than CSM and LCSM. Conclusion: The accurate determination of AID and SID of AA in CSM and LCSM for broilers and layers benefits the application of CSM and LCSM in chicken diets. The cottonseed by-products CSM or LCSM showed the species-specific AA digestibility values for broilers and layers.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.6
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• pp.830-835
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• 2004

An experiment of 10 weeks duration was carried out to study the influence of supplemental effective microorganism (EM) culture, yeast culture and enzymes on nutrient digestibility and gut microflora in rabbit gastrointestinal (GI) tract. Twenty four eight to nine weeks old, New Zealand White rabbits were allotted to four dietary treatments; a basal (control) feed, basal feed supplemented with either EM (1%), yeast culture or enzymes (400 ppm). Nutrient flow in digesta and their digestibility at ileum, caecum, colon and in the total tract as well as gut microflora distribution were studied. Feed dry matter was diluted from 92% to about 14% up to the ileum and about 95% of this water was reabsorbed by the colonic rectal segment followed by caecum (25%). EM and yeast improved protein digestibility at a lower rate than enzymes. Ileal, caecal, colonic and total tract digestibility of crude protein with enzymes were higher by 10.8, 9.4, 11.3 and 10.7%, respectively, as compared to the control. Yeast and enzymes increased crude fiber digestibility at ileum, caecum, colon and in the total tract by 8.5, 9.6, 9.0 and 8.3%, respectively, while EM improved them at a lower rate. Irrespective of treatments, total tract digestibility of crude protein (0.698-0.773) and fiber (0.169-0.183) were greater (p<0.05) than the ileal digestibility. Even though a post-caecal protein digestibility was observed, fiber digestion seemed to be completed in the caecum especially with yeast and enzymes. High precaecal digestibility of crude fiber (97%) and protein (95%) were observed even without additives probably due to caecotrophy. EM and yeast culture promoted the growth of lactic acid bacteria especially in the caecum but they did not influence gut yeast and mould. Present findings reveal that even though rabbits digest nutrients efficiently through hind gut fermentation, they can be further enhanced by EM, yeast and enzymes. Of the three additives tested, enzymes found to be the best.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.3
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• pp.249-254
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• 1998

Two experiments were conducted to evaluate the effects of processing of barley on the growth performance and ileal and fecal digestibility of growing pigs. In Exp. 1, a total of 20 cannulated pigs (10.80 kg BW) were allotted to four treatments. Treatments were coarse ground barley as a control (CON), finely ground barley (FINE), extruded barley (EXT) and enzyme supplemented coarse ground barley (ENZ). In Exp. 2, a total of 100 growing pigs (36.50 kg BW) were allocated to the same treatments in completely randomized block design based on sex and body weight. In the first trial, pigs fed extruded barley showed significantly higher crude protein digestibility over pigs fed finely ground barley (p < 0.05). Pigs fed finely ground barley generally showed lower nutrients digestibility. Extrusion and ${\beta}$-glucanase supplementation showed a trend to improve nutrients digestibility. However, fine grinding rather reduced nutrients digestibility. The similar trend was found in the digestibility of essential amino acids. Fine grinding of barley significantly reduced amino acids digestibility. Extrusion and enzyme supplementation were found to improve amino acids digestibility of barley in growing pigs. In the growth trial, pigs fed extruded barley grew significantly faster than any other processed barley fed pigs. And extrusion of barley significantly improved feed/gain of pigs (p < 0.05). Fine grinding of barley and enzyme supplementation did not improve growth performance of pigs. In conclusion, fine grinding and enzyme supplementation does not appear to be an economical feed processing for growing pigs when barley is employed in the diets, while extrusion can be recommended as an effective feed processing technique for barley.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.3
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• pp.316-320
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• 2001

Sixteen growing crossbred Anglo-Nubian goats, 10-12 months old, pre-experimental average body weights of $18.3{\pm}0.28kg$ were randomly allotted to four diets to investigate the efficiency of utilisation of diets containing increasing levels of desiccated coconut waste meal (DCWM) in the tropical environment of Samoa, South Pacific region. The four diets were designated as 1, 2, 3 and 4. Diet 1 that has no DCWM served as the control, while diets 2, 3 and contained different levels of DCWM. Voluntary concentrate intake, growth rate, feed efficiency and nutrient digestibility coefficients were measured. Gross energy (GE) and organic matter (OM) values of the diets increased linearly with increase in the levels of DCWM in the diets. Voluntary concentrate intake decreased with increasing proportion of DCWM in the diets. Forage intake increased with increase in the levels of DCWM diets offered to the growing goats. Total voluntary feed intakes were statistical significant (p<0.05) and the goats on diet 4 had the least intake followed by those on diet 3. Goats an diets 1 and 2 were different in total feed intake but not at a significant level (p>0.05). Feed efficiency (FE) followed the trend of voluntary concentrate intake and daily live weight gains. Dry matter digestibility (DMD) was significantly different (p<0.05) among the goats offered the different diets. DMD improved with increasing levels of DCWM. The goats accepted all, the diets that were compounded with the different levels of dried DCWM and this seems to suggest that DCWM have no deleterious effects. However, the best level at which dried DCWM could replace brewers dried grains in the diets of growing goats is at 38.5% (diet II). Based on voluntary feed intake, live-weight gain and apparent nutrient digestibility coefficients of the goats it could be concluded that DCWM based diets merits further attention as a locally available feed source in ruminants nutrition in the Pacific Island countries where feed availability is seasonal.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.6
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• pp.847-854
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• 2015

The objective of this study was to determine the effects of graded inclusions of wheat bran (0%, 9.65%, 48.25% wheat bran) and two growth stages (from 32.5 to 47.2 kg and 59.4 to 78.7 kg, respectively) on the apparent ileal digestibility (AID), apparent total tract digestibility (ATTD) and hindgut fermentation of nutrients and energy in growing pigs. Six light pigs (initial body weight [BW] $32.5{\pm}2.1kg$) and six heavy pigs (initial BW $59.4{\pm}3.2kg$) were surgically prepared with a T-cannula in the distal ileum. A difference method was used to calculate the nutrient and energy digestibility of wheat bran by means of comparison with a basal diet consisting of corn-soybean meal (0% wheat bran). Two additional diets were formulated by replacing 9.65% and 48.25% wheat bran by the basal diet, respectively. Each group of pigs was allotted to a $6{\times}3$ Youden square design, and pigs were fed to three experimental diets during three 11-d periods. Hindgut fermentation values were calculated as the differences between ATTD and AID values. For the wheat bran diets, the AID and ATTD of dry matter (DM), ash, organic matter (OM), carbohydrates (CHO), gross energy (GE), and digestible energy (DE) decreased with increasing inclusion levels of wheat bran (p<0.05). While only AID of CHO and ATTD of DM, ash, OM, CHO, GE, and DE content differed (p<0.05) when considering the BW effect. For the wheat bran ingredient, there was a wider variation effect (p<0.01) on the nutrient and energy digestibility of wheat bran in 9.65% inclusion level due to the coefficient of variation (CV) of the nutrient and energy digestibility being higher at 9.65% compared to 48.25% inclusion level of wheat bran. Digestible energy content of wheat bran at 48.25% inclusion level (4.8 and 6.7 MJ/kg of DM, respectively) fermented by hindgut was significantly higher (p<0.05) than that in 9.65% wheat bran inclusion level (2.56 and 2.12 MJ/kg of DM, respectively), which was also affected (p<0.05) by two growth stages. This increase in hindgut fermentation caused the difference in ileal DE (p<0.05) to disappear at total tract level. All in all, increasing wheat bran levels in diets negatively influences the digestibility of some nutrients in pigs, while it positively affects the DE fermentation in the hindgut.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.10
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• pp.1407-1413
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• 2000

Oil palm frond (OPF) is a new non-conventional fibrous feed for ruminants. Evaluation on the nutritive values and digestibility of OPF was carried out using goats. In a completely randomised design, 20 local male goats were assigned to evaluate fresh and different types of processed OPF. A 60 day feeding trial was done to determine the digestible nutrient intake of fresh, ensiled and pelleted OPF and its response on live weight gain of goat. The pelleting of OPF increased (p<0.05) intake compared to fresh or ensiled OPF. The OPF based mixed pellet (50% OPF with 15% palm kernel cake, 6% rice bran, 6% soybean hull, 15% molasses, 2% fishmeal, 4% urea, 1.5% mineral mixture and 0.5% common salt) increased (p<0.05) nutrient intake, digestibility and reduced feed refusals. The mixed pellet also increased digestible dry matter intake (DDMI) and digestible organic matter intake (DOMI) at 80% and 63% level respectively than the fresh OPF. The increased digestible nutrient intake on the OPF based mixed pellet, resulted in increased live weight gain of goats. Furthermore, OPF has a good potential as a roughage source when it is used with concentrate supplement. OPF based formulated feed in a pelleted form could be used as a complete feed for intensive production of goat and other ruminants.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.11
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• pp.1607-1614
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• 2011

This trial was conducted to determine the effects of including canola protein concentrate in diets fed to broiler chickens on nutrient digestibility and broiler performance (0-21 days). A total of 180, day-old, male broiler chicks weighing an average of 52.8${\pm}$0.6 g were assigned to one of six dietary treatments in a completely randomized design. The control diet was based on corn and soybean meal and contained 15% canola meal. The experimental diets contained 3, 6, 9, 12 or 15% canola protein concentrate added at the expense of canola meal. There were five birds per pen and six replicate pens per treatment. Feed and water were available ad libitum throughout the 21-day experiment. Chromic oxide (0.35%) was added to all diets as a digestibility marker and was fed throughout the experimental period. The digestibility of dry matter, energy and phosphorus increased linearly (p<0.01) with increasing levels of canola protein concentrate. Although nutrient digestibility was higher for birds fed diets containing canola protein concentrate, these improvements did not translate into improvements in broiler performance. Weight gain was unaffected (p = 0.24) by level of canola protein concentrate. Feed intake was significantly increased (p<0.01) with the result that feed conversion tended to be poorer (p = 0.07) for birds fed diets containing canola protein concentrate. Mortality was also unaffected (p = 0.56) by dietary treatment.

• Journal of Aquaculture
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• v.21 no.2
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• pp.89-95
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• 2008

Flounder were fed a reference diet and test diets containing various feed ingredients: mackerel fish meal, meat meal, soybean meal, wheat flour, wheat gluten, corn gluten meal and brewer's yeast. Apparent digestibility was determined using a reference diet with 0.5% chromic oxide indicator and test diets contained 70% reference diet and 30% of the feed ingredient being evaluated. Apparent digestibility coefficients for amino acid and energy in the reference and test diets were determined, and digestibility coefficients for the test ingredients were calculated based on differences in the digestibility of test diets relative to the reference diet. The fish averaging 300 g were held in 2000 L tanks at a density of 20 fish per tank. Feces were collected from three replicate groups of fish using a fecal collection column attached to fish rearing tank. Apparent total amino acids digestibilities(90-95%) of mackerel fish meal, soybean meal, wheat gluten, corn gluten meal and brewer's yeast were higher than those of meat meal and wheat flour(P<0.05). Apparent energy digestibilities(86-98%) of mackerel fish meal, meat meal, soybean meal, wheat gluten and corn gluten meal were significantly higher(P<0.05) than those of wheat flour and brewer's yeast. These results provide useful information about nutrient and energy utilization for flounder.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.3
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• pp.396-401
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• 2006

A total of 72 castrated, crossbred ($Landrace{\times}Yorkshire$) piglets ($5.7{\pm}0.7kg$ BW) were allotted to one of three treatments including: a dry crumbled feed fed for 40 days; liquid feed provided for 10 days followed by dry crumbled feed for 30 days; and liquid feed provided for 20 days followed by dry crumbled feed for 20 days. Liquid feed was produced fresh each day by mixing water with dry crumbled feed at a ratio of 3:1. Pigs fed liquid feed for 10 days had a higher weight gain during the first 10 days (+16.8%; p = 0.01) and over the entire experimental period (+4.9%; p = 0.07) than pigs offered dry feed. Pigs fed liquid feed for 20 days also had a higher weight gain during the period from d 0 to 10 (+12.8%; p = 0.01), from d 10 to 20 (+8.5%; p = 0.06) and from d 0 to 20 (+9.7%; p = 0.01) than pigs offered dry feed. Pigs fed liquid feed for the first 10 days had higher feed intakes from d 0 to 10 (+22.6%; p = 0.01) and from d 0 to 40 (+5.3%; p = 0.02) than pigs offered dry feed. Pigs fed liquid feed for the first 20 days had a higher feed intake from d 0 to 10 (+21.8%; p = 0.01), from d 10 to 20 (+10.6%; p = 0.06), from d 0 to 20 (+14.6%; p = 0.01) and from d 0 to 40 (+6.6%; p = 0.02) than pigs offered dry feed. Feed conversion from d 0 to 40 tended to be poorer for pigs fed liquid feed during the first 20 days (p = 0.08) indicating an increase in feed wastage with liquid feeding. Nutrient digestibility at day 10 was unaffected by dietary treatment. However, at day 30, pigs fed liquid feed for 10 days had higher digestibility of dry matter (p = 0.08), energy (p = 0.10), crude protein (p = 0.03) and neutral detergent fibre (p = 0.05) than pigs fed liquid feed for 20 days. In conclusion, liquid feeding for 10 or 20 days increased the performance of weaned pigs during the specific time period that liquid feeding occurred but there were no carry over effects into subsequent production periods.

• Korean Journal of Agricultural Science
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• v.47 no.2
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• pp.229-237
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• 2020

This study assessed the effects of a dietary non-genetically modified organism (non-GMO) source on growth performance and nutrient digestibility of grower-finisher pigs. The dietary treatments were 1) rice-soybean meal-based control diet and 2) rice and non-GMO soybean meal-based diet. In the experiment 1, 60 growing pigs (initial body weight [BW] = 23.76 ± 3.42 kg) were randomly assigned to 1 of 2 dietary treatments with 6 pigs·pen^-1 (5 replications) for 6 weeks. In experiment 2, 48 finishing pigs (initial BW = 64.31 ± 6.17 kg) were randomly assigned to 1 of 2 treatment groups with 4 pigs·pen^-1 (6 replications) for 6 weeks. Measurements were the average daily gain (ADG), average daily feed intake (ADFI), gain-to-feed ratio (G : F), and nutrient digestibility. The growth performance was measured at the beginning and end of each period. The apparent total tract digestibility (ATTD) was determined by chromium oxide as an indigestible marker during the last 7 days of each experiment. During the grower period, pigs fed the diet containing the non-GMO soybean meal had a higher (p < 0.05) ADFI than those fed the control diet; however, there were no differences between the dietary treatments in the ADG, G : F, and ATTD. Moreover, the dietary treatments did not affect the ATTD and growth performance of the finishing pigs. In conclusion, the inclusion of non-GMO soybean meal in the diet had no negative effects on the growth rate and nutrient digestibility, indicating that non-GMO soybean meal can be used in diet formulations with other feed ingredients and be a substitute for conventional soybean meal.