• Journal of Microbiology
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• 제42권1호
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• pp.1-8
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• 2004

Changes in the bacterial populations of a 5-stage biological nutrient removal (BNR) process, with a step feed system for wastewater treatment, were monitored by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S ribosomal DNA fragments. DGGE analysis indicated seasonal community changes were observed, however, community profiles of the total bacteria of each reactor showed only minor differences in the samples obtained from the same season. The number of major bands was higher in the summer samples, and decreased during the winter period, indicating that the microbial community structure became simpler at low temperatures. Since the nitrogen and phosphate removal efficiencies were highly maintained throughout the winter operation period, the bacteria which still remaining in the winter sample can be considered important, playing a key role in the present 5-stage BNR sludge. The prominent DGGE bands were excised, and sequenced to gain insight into the identities of the predominant bacterial populations present, and most were found to not be closely related to previously characterized bacteria. These data suggest the importance of culture-independent methods for the quality control of wastewater treatment.

• 한국양식학회지
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• 제13권3호
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• pp.259-265
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• 2000

Four experimental diets were formulated to contain white fish meal (WFM) andvor BAIFA-M as the main animal protein source to determine the optimum BAIFA-M level to replace WFM protein. The diets contained 100% WFM + 0% BAIFA-M in the diet M$_0$ (control) . 70%, WFM+30%, BAIFA-M in the diet M$_{30}$, 55 % WFM+45 % BAIFA-M in the diet M$_{45}$, ; 40 % WFM+60 % BAIFA-M in the diet M$_{60}$. The four groups of rockfish (232 g), receiving the four different diets, were fed for 8 weeks. Groups receiving M$_0$, M$_{30}$ and M$_{45}$/ diets did not show significant (P>0.05) difference in growth and feed efficiency; however, the group fed on M$_{60}$/ showed significantly slower growth and lower feed efficiency than the others. Body composition showed no significant difference among the four dietary groups. WFM protein can be replaced by BAIFA-M up to 45 % in the diet of the rockfish.

• Asian-Australasian Journal of Animal Sciences
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• 제15권10호
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• pp.1458-1468
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• 2002

There is increasing interest in exploiting natural products as feed additives to solve problems in animal nutrition and livestock production. Essential oils and saponins are two types of plant secondary compounds that hold promise as natural feed additives for ruminants. This paper describes recent advances in research into these additives. The research has generally concentrated on protein metabolism. Dietary essential oils caused rates of NH$_3$ production from amino acids in ruminal fluid taken from sheep and cattle receiving the oils to decrease, yet proteinase and peptidase activities were unchanged. Hyper-ammonia-producing (HAP) bacteria were the most sensitive of ruminal bacteria to essential oils in pure culture. Essential oils also slowed colonisation and digestion of some feedstuffs. Ruminobacter amylophilus may be a key organism in mediating these effects. Saponin-containing plants and their extracts appear to be useful as a means of suppressing the bacteriolytic activity of rumen ciliate protozoa and thereby enhancing total microbial protein flow from the rumen. The effects of some saponins seems to be transient, which may stem from the hydrolysis of saponins to their corresponding sapogenin aglycones, which are much less toxic to protozoa. Saponins also have selective antibacterial effects which may prove useful in, for example, controlling starch digestion. These studies illustrate that plant secondary compounds, of which essential oils and saponins comprise a small proportion, have great potential as 'natural' manipulators of rumen fermentation, to the potential benefit of the farmer and the environment.

• Asian-Australasian Journal of Animal Sciences
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• 제8권5호
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• pp.455-462
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• 1995

Arbor Acres broiler chickens (N=288) with an average initial weight of 59.4 g were fed diets varying in protein and lysine (80, 100, 120% of NRC; 100, 120% of NRC, 1984) in order to investigate the effects of supplemental chromium picolinate on growth performance, nutrient utilizability, carcass composition, serum traits and in vitro protein synthesis. Six replicates of eight chicks were grouped into one treatment Six chicks were sacrificed from each treatment for carcass analysis, and six additional chicks were chosen and dissected for in vitro culture of liver tissue. Body weight gain, feed intake, feed conversion, mortality, carcass composition and serum glucose, HDL/cholesterol ratio, serum triglyceride and serum nonesterified fatty acid appeared to be affected by either the level of dietary crude protein or lysine when supplemented with 200 ppb chromium picolinate (p < 0.05). Retained and secreted proteins in liver acinar cell cultured in vitro were not affected by dietary lysine level but affected by dietary protein level when added with 200 ppb chromium picolinate.

• 한국수산과학회지
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• 제40권4호
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• pp.183-186
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• 2007

The effect of live food and commercial diet on sweetfish Plecoglossus altivelis culture was assessed by determining the daily growth rate and feed conversion ratio. After feeding for 8 weeks, the Moina macrocopa diet resulted in a higher growth rate of 5.101 compared to commercial (3.441) and Artemia (2.729) diets. In addition fish fed the M. macrocopa diet had the weight gain, averaging 41.29 g at the end of the experiment. The content of n-3 highly unsaturated fatty acids (HUFA) was highest in the commercial diet, while free fatty acids were not detected in the M. macrocopa diet. Triglycerides, the energy source in fishes, constituted 40.0% of the M. macrocopa diet, which was significantly higher than in the commercial and Artemia diets at 20.0 and 12.0%, respectively These results indicate that freshwater M. macrocopa can be used as feed for sweetfish larvae, even without docosahexaenoic acid (DHA) enrichment from Artemia.

• Fisheries and Aquatic Sciences
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• 제11권4호
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• pp.190-194
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• 2008

We estimated the digestibility and growth rate of juvenile olive flounder (Paralichthys olivaceus) on three diets: raw fish-based moist pellets, moist pellets, and extruded pellets. The diets were created using commercially available methods, and a basic formulated powder. A reference diet was used to compare feed digestibility and the fish growth rate achieved using the experimental diets. The apparent digestibility coefficients (ADCs) of protein for raw fish-based moist pellets and moist pellets were significantly higher (P<0.05) than those for extruded pellets and the reference diet. The ADCs of nitrogen-free extracts (NFE) of extruded pellets and moist pellets were significantly higher (P<0.05) than those for raw fish-based moist pellets and the reference diet. Fish that were fed with the raw fish-based moist pellets showed the greatest weight gain (452.4%), which was not significantly different from that of fish fed with extruded pellets (414.4%; P>0.05). These results indicate that the higher protein efficiency in fish fed with extruded pellets can result in good growth performance within flounder culture systems.

• Journal of Microbiology and Biotechnology
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• 제18권5호
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• pp.908-912
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• 2008

To achieve a higher succinic acid productivity and evaluate the industrial applicability, this study used Mannheimia succiniciproducens LPK7 (knock-out: ldhA, pflB, pta-ackA), which was recently designed to enhance the productivity of succinic acid and reduce by-product secretion. Anaerobic continuous fermentation of Mannheimia succiniciproducens LPK7 was carried out at different glucose feed concentrations and dilution rates. After extensive fermentation experiments, a succinic acid yield and productivity of 0.38 mol/mol and 1.77 g/l/h, respectively, were achieved with a glucose feed concentration of 18.0 g/l and $0.2\;h^{-1}$ dilution rate. A similar amount of succinic acid production was also produced in batch culture experiments. Therefore, these optimal conditions can be industrially applied for the continuous production of succinic acid. To examine the quantitative balance of the metabolism, a flux distribution analysis was also performed using the metabolic network model of glycolysis and the pentose phosphate pathway.

• Fisheries and Aquatic Sciences
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• 제27권8호
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• pp.475-487
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• 2024

Larviculture of commercially important aquaculture species faced limitations associated to the incomplete understanding of larval nutrition and the inability to total replacement of live feeds by formulated diets at the early larval stage. The main challenges to alternatives of live feed in larval fish culture are related to the inherent behaviors of the larvae and the incomplete knowledge and practice leading to the inefficiency of using micro diets. Although significant achievement has been reached in the complete replacement of live feeds by formulated micro diets in freshwater species and marine shrimps, its success is far from complete in marine finfishes. However, recent progress in biotechnological advances in manufacturing process and advanced knowledge of the nutritional necessities of larvae indicated improvements in the field. A range of technologies in the manufacturing of micro diets for larval fish are in place currently. To this end, several achievements of substituting live feeds with formulated micro diets at later stages of larval development have been reported by various researchers providing a clue on the prospects for the future. Therefore, the objective of this review is to compile existing information on the challenges of substituting live feeds by formulated diets in the past and prospects for future development.

• 한국유기농업학회지
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• 제23권3호
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• pp.523-534
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• 2015

본 연구는 미생물제제의 일종인 yeast culture 및 코팅 처리된 vitamin-C의 첨가 급여를 통해 비육 말기 Holstein 거세우의 증체 및 도체형질에 미치는 영향을 조사하기 위한 목적으로 실시하였다. 평균체중이 $714{\pm}13.60kg$인 18개월령 Holstein 거세우 24두를 공시동물로 이용하였으며 각각 8두씩 3개 시험구로(대조구, MC급여구, CVC급여구) 완전임의 배치하여 출하 전 3개월간 사양시험을 실시하였다. MC급여구는 yeast culture를 일일 30 g, 코팅 처리된 vitamin-C는 일일 10 g씩 매일 아침 사료급여 시 top dressing형태로 급여하였다. 일당증체량은 MC급여구가 가장 높았으며 사료요구율은 MC급여구와 CVC급여구에서 가장 낮았다. 등지방두께, 배체장근 및 육량은 시험구간 유의차가 없었다. 지육중량과 성숙도는 MC급여구에서 가장 높았으며, 지방색은 CVC급여구에서 가장 높게 나타났다. 이상의 결과를 종합해 보면 출하 3개월 전 Holstein 거세우에게 항생제 대체제로 미생물제제인 yeast culture 또는 코팅 처리된 vitamin-C의 급여는 육질에는 큰 효과가 없으나 육량, 지육중량, 사료요구율 등에 긍정적인 영향을 줄 수 있을 것으로 판단된다. 아울러 미생물제제와 vitamin-C를 함께 급여하면 더욱 좋은 효과가 있을 것으로 예상된다.

• 폴리머
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• 제28권1호
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• pp.86-91
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• 2004

생체 이식용 재료로서 제한된 용도를 갖는 기존의 스폰지를 대체하기 위해 히드록시에틸 메타크릴레이트 및 메타크릴산염이 높은 단량체 농도에서 단독 또는 공중합하여 생체적합성 및 생체안정성이 향상된 수화젤을 합성하고 이들의 세포 독성, 세포 부착성, 및 조직 적합성 등을 고찰하였다. 히드록시에틸 메타크릴레이트의 투입 몰비가 증가할수록 세포 독성은 감소하여 0.05의 투입 몰비에서는 거의 세포 독성을 나타내지 않았다. 메타크릴산염이 0.01의 몰비로 투입된 수화젤이 가장 우수한 세포 부착 및 퍼짐을 보인 반면, 메타크릴산염이 0.05 투입된 수화젤은 낮은 세포 부착을 보였다. 메타크릴산염이 공중합된 수화젤은 이식 후 8주가 경과하여도 특별한 이물질 반응의 소견을 보이지 않았다. 따라서 높은 단량체 농도에서 히드록시에틸 메타크릴레이트와 메타크릴산염을 적절한 단량체 투입비로 공중합하여 제조한 수화젤은 탁월한 생체적합성 및 생체안정성을 갖는 생체 이식용 재료로 사용될 수 있다고 사료된다.