• Asian-Australasian Journal of Animal Sciences
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• 제33권2호
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• pp.264-276
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• 2020

Objective: Two experiments were conducted to evaluate tetrabasic zinc chloride (TBZC) on the health of weaned pigs, and to determine the optimal supplemental concentrations and whether dietary TBZC could replace the pharmacological concentrations of dietary zinc oxide (ZnO) to improve growth performance and decrease Zn excretion in weaned pigs. Methods: In Exp. 1, 180 weaned pigs (8.92±1.05 kg body weight [BW]) were randomly assigned to 1 of 5 treatments, including the basal diet containing 125 mg/kg zinc sulfate (ZnSO₄), and the basal diet with 1,200, 1,800, 2,400, or 3,000 mg/kg TBZC supplementation. In Exp. 2, 240 weaned pigs (7.66±1.09 kg BW) were randomly assigned to 1 of 5 treatments, including a negative control diet without Zn supplementation, a positive control diet (2,250 mg/kg ZnO), and 3 experimental diets with different concentrations of TBZC supplementation (1,000, 1,250, and 1,500 mg/kg). Results: In Exp. 1, the average daily gain (ADG), feed efficiency (G:F) and diarrhea incidence responded quadratically (p<0.01) as the TBZC supplemental concentrations increased, and pigs fed 1,200 and 1,800 mg/kg TBZC showed the best growth performance. Moreover, 1,800 mg/kg TBZC supplementation showed the greatest (p<0.01) total antioxidant capacity and glutathione peroxidase activities in liver of pigs. Histopathological examination revealed lesions in heart, liver, lung and kidney, and mild or severe histological lesions mainly occurred with the supplementation of 2,400 and 3,000 mg/kg TBZC. In Exp. 2, 1,000 and 1,250 mg/kg TBZC supplementation in diets significantly (p<0.01) increased ADG and G:F of weaned pigs, reduced Zn excretion in feces, and had no effect on diarrhea-reducing compared to 2,250 mg/kg ZnO supplementation. Conclusion: The TBZC is a potential alternative to ZnO. The recommended concentration of TBZC in weaned pig diets is 1,000 to 1,250 mg/kg.

• Journal of Microbiology and Biotechnology
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• 제10권2호
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• pp.147-153
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• 2000

This study was conducted to compare probiotic activities and physiological functions of Bifidobacterium longum Mk-G7 with weveral commercial and type strains of bifidobacteria. bif. longum MK-G7 showed the highest acid tolerance against HCl and acetic acid, whereas bif. infantis Y-1 showed the lowest acid tolerance and more than 4 log cycles of viable cell count decreased due to acid injuty. Viable cell counts of bifidobacteria strains decreased more than 1.5 log cycles owing to oxygen toxicity, with the exception of Bif. longum MK-G7, Bif. infantis Y-2, Bif. longum Y-3, Bif. longum Y-6, and Bif. longum RD-13 showed the highest bile tolerance, whereas Bif. longum MK-G7 showed a medium level of bile tolerance. Only Bif. longum MK-G7 howed much higher antibiotic resistance against both tetracycline and penicillin-G in the MIC(minimum inhibitory concentration) level of 24.8 mg/I and 0.52mg/I, respectively. Bif longum Y-6, and Bif. bifidum ATCC 29539 showed more than 80% of anti-mutagenicity against NQO(4-nitroquinolinel-oxide). Since the production of cytokines such as $TNF(tumor necrosis factor)-{\alpha}$ and IL (interleukin)-6, and NO(nitric oxide) in the macrophage cell line Raw 264.7 cells increased as Bif. longum MK-G7 cell concentration increased, ti was suggested that Bif. longum MK-G7 is able to enhance immunopotentiating activity in vitro. When freeze-dred Bif. longum MK-G7 was administered to mice at the dose of 1,2,4, and 6 g/kg of body weight, all of the mice survived in all feeding groups, proving the GRAS(generally recognized as safe) status of Bif. longum MK-G7. When fermented milk containing Bif. longum MK-G7 was administered to human volunteers, viable cell count of total bifidobacteria and anaerobes in the feces increased up to 0.5 log cycles more than before the administration. In particular, Bif. logum MK-G7 ingibited the growth of Bacteroides at the level of 1.0-1.5 log cycles.

• Asian-Australasian Journal of Animal Sciences
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• 제15권10호
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• pp.1482-1488
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• 2002

The aim of this study was to explore the effects of fiber sources on gut development and bacterial activity in the gastrointestinal tract of piglets. Eighteen crossbred (Duroc${\times}$Landrace${\times}$Yorkshire) barrows were fed a basal diet based on corn plus soybean meal or similar diets in which a portion of the corn and soybean was replaced by 5% wheat bran or 5% sugar beet pulp. The results indicate that pigs fed diets containing 5% wheat bran or 5% sugar beet pulp had lower liver weights than control pigs (p<0.01). The relative weight of the pancreas in pigs fed diets containing 5% sugar beet pulp was greater than that of control pigs or pigs fed diets containing 5% wheat bran (p<0.05). The pH of the ileal digesta of pigs fed the diet containing 5% wheat bran was higher than that of control pigs or pigs fed the diet containing 5% sugar beet pulp (p<0.05). The lipase activity in the distal jejunum, proximal, and distal ileum of pigs fed the control diet was higher than that of pigs fed the diets containing 5% wheat bran or 5% sugar beet pulp (p<0.05). The concentration of volatile fatty acids anterior to the caecum was greater for the pigs fed the diet containing 5% sugar beet pulp, while the concentration of volatile fatty acids posterior to the ileum was greater for the pigs fed the diet containing 5% wheat bran. This means that sugar beet pulp increased the bacterial fermentation precaecum, while wheat bran increased the bacterial fermentation post-ileum. The concentration of bacterial nitrogen and bacterial protein/total protein in ileal digesta of pigs fed the control diet was higher (p<0.05) than that of pigs fed the diets contained either fiber source. Bacterial protein/total protein in the feces of pigs fed the diet containing 5% sugar beet pulp was higher than that of pigs fed the control diet. This means that inclusion of 5% wheat bran or sugar beet pulp in diets influenced the development of the digestive tract of piglet. The mechanism by which dietary fiber reduced specific activity of lipase needs further consideration. Dietary fiber influenced the bacterial activity in the digestive tract of piglets, sugar beet pulp increased the fermentation in the upper gastrointestinal tract, and while wheat bran increased the fermentation in the lower gastrointestinal tract.

• Asian-Australasian Journal of Animal Sciences
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• 제21권3호
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• pp.434-442
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• 2008

The objective of this study was to determine the optimum ratio of methionine to methionine plus cystine for growing pigs. A nitrogen balance trial was conducted using a total of 21 barrows (Large WhiteLandrace) over two replicates. The initial body weight was $20.36{\pm}1.22kg$ (mean${\pm}$SD) in the first replicate and $23.54{\pm}1.02kg$ (mean${\pm}$SD) in the second. For each replicate, the 21 pigs were randomly assigned to one of seven dietary treatments with three observations per treatment. The diets included a methionine and cystine-deficient basal diet with all other essential nutrients meeting nutrient requirements and six diets formulated with graded levels of DL-methionine (0.00, 0.03, 0.06, 0.10, 0.13, 0.16%) and $L-Cystine{\cdot}HCl{\cdot}H_2O$ (0.19, 0.15, 0.11, 0.07, 0.04, 0.00%). This resulted in ratios of methionine to methionine plus cystine of 41.3, 29.6, 35.3, 41.2, 46.0, 51.6 and 57.5%. Each experimental period lasted 12 days consisting of a seven-day adaptation period followed by a five-day total collection of urine and feces. During the collection period, pigs were fed 900 g/day for the first replicate and 1,200 g/day for the second replicate. The feed was provided in three equal portions at 0800, 1500, and 2200 h daily. Pigs had ad libitum access to water after feeding. There was a linear (p<0.01) and quadratic (p<0.01) effect on daily gain and feed conversion as the ratio of methionine to methionine plus cystine increased. Pigs receiving the diets providing a methionine to methionine plus cystine ratio of 51.6% had the best daily gain and feed conversion. Plasma urea nitrogen was also lowest for this treatment. Nitrogen retention increased (p<0.01) as the relative proportion of methionine increased up to 51.6% and then a downward trend occurred at 57.5%. The quadratic regression model, as well as one- and two- slope regression line models, were used to determine the optimum ratio of methionine to methionine plus cystine. Eliminating the 35.3% methionine to methionine plus cystine treatment resulted in $R^2$ values in excess of 0.92. The optimal ratio of methionine to methionine plus cystine was estimated to be 54.15% for nitrogen retention and 56.72% for plasma urea nitrogen.

• 한국가금학회지
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• 제30권1호
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• pp.61-66
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• 2003

본 연구는 산란계 사료내 게르마늄 흑운모의 첨가가 난각 특성 및 분내 암모니아 함량에 미치는 영향을 조사하기 위하여 실시되었다. 사양시험에 40주령 ISA brown 갈색계 144수를 이용하였으며, 옥수수-대두박 위주 대조사료 (CON), 게르마늄 흑운모 0.5％(GB0.5), 1.0％(GB1.0) 및 1.5％(GB1.5) 첨가구 등4개 처리로 구성되었다. 총 56일간의 사양시험 기간동안, 산란율과 난각강도에서는 처리간에 차이를 보이지 않았다. 그러나 난중은 게르마늄 흑운모의 첨가수준이 증가함에 따라 유의적으로 감소하는 경향을 나타내었다. (P<0.02). 난각두께, 난황색 및 난황계수에서는 처리구간에 차이를 보이지 않았다. 혈장내 중성지질 함량에 있어서는 게르마늄 흑운모의 첨가수준이 증가함에 따라 유의적으로 증가하는 경향을 나타내었다 (P<0.05). 분내 암모니아태 질소 농도는 게르마늄 흑운모의 첨가수준이 증가함에 따라 유의적으로 감소되는 경향을 보였다 (P<0.01). 또한 게르마늄 흑운모의 첨가수준이 증가함에 따라 분내 propionic acid (P<0.01), butyric acid (P<0.05) 그리고 acetic aicd (P<0.04)의 함량이 유의적으로 감소하는 경향을 나타내었다. 결과적으로 산란계 사료내 게르마늄 흑운모의 첨가는 분내 암모니아가스의 발생을 감소시킬 수 있을 것으로 사료된다.

• 한국가금학회지
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• 제34권2호
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• pp.119-128
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• 2007

산란계의 생산성, 계란 품질, 질소와 인의 흡수량과 배설량 및 회장 소화율에 미치는 파이타제의 첨가 효과를 구명하고자 사료에 0, 300, 600 FTU/kg의 파이타제를 첨가하여 4주간 사양 시험 종료후 1주간 대사실험을 실시하였다. 공시동물은 산란율이 유사한 55주령 White Leghorn 산란계 162수$(1.6{\pm}0.3\;kg)$를 공시하였으며, 처리구는 첨가 수준에 따라 P0(대조구), P1(300 FTU/kg), P2(600 FTU/kg)의 3처리구로 나누고, 처리구당 6반복, 반복당 9수씩 완전임의 배치하였다. 산란율은 파이타제 첨가 수준에 따라 증가하였다. 계란 품질은 난중, 난각 두께, 하우유니트와 난황색 모두 처리구간에 차이를 보이지 않았으나(p>0.05), 난각 강도는 파이타제 처리구에서 4주째에 각각 3.86 $kg/cm^2$와 3.85 $kg/cm^2$로 나타나 파이타제 첨가에 따른 차이를 보였다(p<0.05). 질소 배설량과 흡수량은 처리구간에 차이가 없었으나(p>0.05), 인 배설량과 흡수량은 파이타제 첨가구에서 대조구에 비해 배설량이 크게 감소하고 흡수량은 증가하였다(p<0.05). 파이타제 첨가에 따른 영양소와 아미노산의 소화율은 조섬유, 조회분, 칼슘과 인, 필수 아미노산 중 lysine, methionine, phenylalanine, 비필수 아미노산 중 alanine, cystine, glutamic acid, glycine, tyrosine의 소화율을 유의적으로 증가시켰다(p<0.05). 이 실험 결과 산란계에 미생물유래 파이타제 급여는 산란율을 향상시키고, 인 배설량을 낮추며, 흡수량을 증가시키지만, 계란 품질에 미치는 영향이 없었고, 소화율에 대한 영향은 추후의 연구가 필요하다고 사료된다.

• 환경위생공학
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• 제7권2호
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• pp.111-128
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• 1992

According to the increase of population and industrialization, the quality of our drinking water are becoming worse by the contamination of resources, production of THM and other halogenated hydrocarbons during the purifying process, the problem of corroded water supplying pipeline, and the water reservoir tanks, Many people choose groundwater to drink instead of city tap water, but sometimes we get report about groundwater contamination by wastes, swage, septic tank, etc. It is reported that in U. S. over 20% of population are drinking groundwater, but U. S EPA reported the groundwater contamination by pesticides, herbicides, fungicides, fertilizer, and various chemical substances. Craun, et at announced the groundwater contamination by bacteria which are related with poor installation of septic tank. Johnson and Kross mentioned aboutmethemoglobinemia by NO3-N originated from human and animal feces, organic chemicals, and fertilizer, and as the results the infant mortality could be risen. Some scientist also reported the high concentration of metals in groundwaters and some cation and anions, and volatile organic compou nds. Authors investigated 80 groundwaters in urban, agricultural, and industrial area during last 3 month(June - August) to check any drinking water quality parameters are exceeding the standards. The results were as follow. 1, The average value of ammonia nitrate were within the standard, but 11.76% of urban area were exceeded the 10 rpm standard, in agricultural area 42.3175 were exceeded, and in industrial area 20.2% were exceeded the drinking water standard of 10 ppm. the highest concentration was 29.37 ppd in industrial area. 2. The mean value of metals is not exceeded the standard, but there were some groundwater whose Mn value was 0.424 ppm(standard is 0,3 ppm) in urban area, 0.737 rpm in agricultural area, and 5.188 ppm in industrial area. The highest Zn value was 1.221 ppm (standard is 1.0 ppm)was found in industrial area. 3. The percentage of contamination by general bacteria was 8.82% in urban area, 15.38% in agricultural area, and 15.00% in industrial area. Escherichia coil group was also contaminated by 35.29% in urban area, 30.76% in agricultural area, and 30.00% in industrial area. 4, The pH value was within the standard which means there was no influence by acid or alkali chemicals, nor acid rain Through the above results, all the groundwater should be tested to check the safety for drinking water and should make some alternative methods suitable for drink.

• Asian-Australasian Journal of Animal Sciences
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• 제20권4호
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• pp.556-562
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• 2007

A total of 126 crossbred weanling pigs (average body weight of $6.3{\pm}0.3$ kg) were used to investigate the effect of chito-oligosaccharide (COS) on growth performance, nutrient digestibility, pH of gastro-intestinal tract (GI), intestinal and fecal microflora of young piglets. Pigs were allocated to three dietary treatments based on body weight and gender in a single factorial arrangement. Treatments were control (No COS), T1 (0.2% COS during starter (6-13 kg) and 0.1% COS during grower (13-30 kg) phases, and T2 (0.4% COS during starter (6-13 kg) and 0.3% COS during grower (13-30 kg) phases, respectively. Each treatment had 3 replicates and 14 pigs were raised in each pen. COS is a low molecular weight water-soluble chitosan that can be obtained from chitin of the crab shell after deacetylation with concentrated sodium hydroxide at high temperature and then further decomposition by chitosanase enzyme in the presence of ascorbic acid. For the starter and grower periods, there were no significant differences (p>0.05) in average daily gain (ADG) and feed to gain ratio among treatments. However, during the overall period (6-30 kg), T2 showed better (p<0.05) feed to gain ratio than other treatments. A digestibility study was conducted at the end of grower phase which showed improvement (p<0.05) in DM and crude fat digestibility in T2 over the control. At 25 kg body weight, 6 pigs per treatment (2 per replicate) were sacrificed to determine the effect of diets on pH and microbial count at different sections of the GI tract. The pH of the cecal contents in pigs fed 0.1% COS was higher (p<0.05) than in the other treatments. Total anaerobic bacterial number increased from cecum to rectum in all treatments. The weekly total bacterial counts showed higher (p<0.05) in feces of pigs fed COS than that of untreated pigs at the $8^{th}$ week. The number of fecal E. coli in untreated pigs at $4^{th}$ wk was 7.35 log CFU/g compared to 6.71 and 6.54 log CFU/g in 0.1 and 0.3% COS-treated pigs, respectively. Similarly, at $8^{th}$ wk, fecal clostridium spp. were lower in pigs fed 0.3% COS (5.43 log CFU/g) than in untreated pigs (6.26 log CFU/g). In conclusion, these results indicated that chito-oligosaccharide could improve feed efficiency in young pigs and inhibited the growth of harmful bacteria.

• Asian-Australasian Journal of Animal Sciences
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• 제23권10호
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• pp.1340-1347
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• 2010

To study the effects of ${\alpha}$-galactosidase (${\alpha}$-Gal) supplementation on performance and energy metabolism, 216 Arbor Acres male broilers were placed in 36 cages of 6 birds each and allotted to 4 diets for 42 d, with 0-21 d as starter period and 22-42 d as grower period. The 4 diets were based on corn non-dehulled soybean meal in a $2{\times}2$ factorial arrangement, with 2 levels of ${\alpha}$-Gal (0 vs. 60 U/kg feed) and 2 levels of ME (normal metabolizable energy (NME) and low metabolizable energy (LME)). Bird performance was obtained at 21 and 42 d of age with samples of feces collected for nutrient digestibility from 19-21 d and 40-42 d. At 21 and 42 d, 1 bird from 6 cages of each treatment was killed to determine liver weight, intestinal pH and chyme viscosity. With the addition of ${\alpha}$-Gal the 42 d body weight (BW) and 0-42 d average daily gain (ADG) were significantly improved (p<0.05). Average daily feed intake (ADFI) of birds fed the LME diet was significantly increased compared to those fed the NME diet during starter (p<0.01) and grower (p<0.05) periods and overall (p<0.01). There was an interaction of ${\alpha}-Gal{\times}ME$ on 0-21 d ADFI (p<0.01). Supplementation of ${\alpha}$-Gal significantly improved (p<0.01) feed efficiency during the grower period and overall. Feed efficiency of birds fed the LME diet was significantly decreased (p<0.05) compared to those fed the NME diet during the starter period and overall. With the addition of ${\alpha}$-Gal apparent metabolizable energy (AME) was improved (p<0.01) by 2.1% and 1.8% during starter and grower periods, respectively. There was a main effect (p<0.05) of ${\alpha}$-Gal on the digestion of neutral detergent fiber (NDF) during the starter period and crude protein (CP), NDF and acid detergent fiber (ADF) during the grower period. With the addition of ${\alpha}$-Gal, the relative weight of liver was reduced (p<0.01) during the two phases. The duodenal and jejunal pH were significantly decreased (p<0.01) with the supplementation of ${\alpha}$at the two phases. ${\alpha}$-Gal addition reduced (p<0.01) chyme viscosity of the ileum during the starter and grower periods. Overall, ${\alpha}$-Gal showed a major effect on nutrient efficiency, improved ADG and feed efficiency, whereas LME decreased feed efficiency. The incorporation of ${\alpha}$-Gal into a LME diet could at least partially offset ME deficiency of non-dehulled soybean meal.

• Asian-Australasian Journal of Animal Sciences
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• 제26권6호
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• pp.827-830
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• 2013

To evaluate the effects of feeding four different levels of starter in male Holstein dairy calves, a completely randomized study was conducted, using 28 calves with initial body weight of $40.5{\pm}2.4$ kg. The animals were fed iso-nitrogenous starter and were weaned when they consumed 350, 500, 650 and 800 g/d of starter for 3 d consecutively. Starter and water were available ad-libitum throughout the experiment. Body weight at pre-weaning (less than 5 wk) and post-weaning (8 wk) was lower in calves that received 350 g/d of starter than in the other treatments (p<0.05). Feed conversion ratio (FCR) was the highest among all treatments in pre-weaning period (p<0.05). Dry matter intake (DMI) at weaning and total DMI was higher in that calves received 800 g/d of starter compared with other treatments (p<0.05). Calves fed 350 and 500 g/d of starter were weaned earlier (p<0.05) and showed lower milk consumption (kg, DM) compared with other treatments whereas no significant difference was observed between calves fed 350 and 500 g/d of starter (p>0.05). Dry matter, organic matter and crude protein digestibilities were lower in calves that received 350 g/d of starter compared with other treatments (p<0.05). No differences were observed in acid detergent and neutral detergent fiber digestibility among all treatments (p>0.05).Treatments had no significant effect on time of starting rumination, respiratory score, and days of drug administration for pneumonia. There were no meaningful differences in feces, fecal odor scores, body temperature, and days of drug administration for diarrhea among all treatments (p>0.05). Total dry matter intake at the end of experiment showed no significant difference among calves fed 600 and 800 g/d of starter, but calves fed 350 and 500 g/d of starter showed more dry matter (DM) intake than calves in the 600 and 800 g/d groups (p<0.05).