Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of α-Galactosidase Supplementation on Performance and Energy Metabolism for Broilers Fed Corn-non-dehulled Soybean Meal Diets

  • Zhang, Bo (State Key Laboratory of Animal Nutrition, China Agricultural University) ;
  • Cao, Yunhe (State Key Laboratory of Animal Nutrition, China Agricultural University) ;
  • Chen, Yiqun (State Key Laboratory of Animal Nutrition, China Agricultural University) ;
  • Li, Yihang (State Key Laboratory of Animal Nutrition, China Agricultural University) ;
  • Qiao, Shiyan (State Key Laboratory of Animal Nutrition, China Agricultural University) ;
  • Ma, Yongxi (State Key Laboratory of Animal Nutrition, China Agricultural University)
  • Received : 2010.03.04
  • Accepted : 2010.04.05
  • Published : 2010.10.01
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Abstract

To study the effects of ${\alpha}$-galactosidase (${\alpha}$-Gal) supplementation on performance and energy metabolism, 216 Arbor Acres male broilers were placed in 36 cages of 6 birds each and allotted to 4 diets for 42 d, with 0-21 d as starter period and 22-42 d as grower period. The 4 diets were based on corn non-dehulled soybean meal in a $2{\times}2$ factorial arrangement, with 2 levels of ${\alpha}$-Gal (0 vs. 60 U/kg feed) and 2 levels of ME (normal metabolizable energy (NME) and low metabolizable energy (LME)). Bird performance was obtained at 21 and 42 d of age with samples of feces collected for nutrient digestibility from 19-21 d and 40-42 d. At 21 and 42 d, 1 bird from 6 cages of each treatment was killed to determine liver weight, intestinal pH and chyme viscosity. With the addition of ${\alpha}$-Gal the 42 d body weight (BW) and 0-42 d average daily gain (ADG) were significantly improved (p<0.05). Average daily feed intake (ADFI) of birds fed the LME diet was significantly increased compared to those fed the NME diet during starter (p<0.01) and grower (p<0.05) periods and overall (p<0.01). There was an interaction of ${\alpha}-Gal{\times}ME$ on 0-21 d ADFI (p<0.01). Supplementation of ${\alpha}$-Gal significantly improved (p<0.01) feed efficiency during the grower period and overall. Feed efficiency of birds fed the LME diet was significantly decreased (p<0.05) compared to those fed the NME diet during the starter period and overall. With the addition of ${\alpha}$-Gal apparent metabolizable energy (AME) was improved (p<0.01) by 2.1% and 1.8% during starter and grower periods, respectively. There was a main effect (p<0.05) of ${\alpha}$-Gal on the digestion of neutral detergent fiber (NDF) during the starter period and crude protein (CP), NDF and acid detergent fiber (ADF) during the grower period. With the addition of ${\alpha}$-Gal, the relative weight of liver was reduced (p<0.01) during the two phases. The duodenal and jejunal pH were significantly decreased (p<0.01) with the supplementation of ${\alpha}$at the two phases. ${\alpha}$-Gal addition reduced (p<0.01) chyme viscosity of the ileum during the starter and grower periods. Overall, ${\alpha}$-Gal showed a major effect on nutrient efficiency, improved ADG and feed efficiency, whereas LME decreased feed efficiency. The incorporation of ${\alpha}$-Gal into a LME diet could at least partially offset ME deficiency of non-dehulled soybean meal.

Keywords

References

  1. Almirall, M., M. Francesch, A. M. Perez-Venderell, J. Brufau andE. Esteve-Garcia. 1995. The differences in intestinal viscosity produced by barley and β-glucanase alter digesta enzyme activities and ileal nutrient digestibilities more in broiler chicks than in cocks. J. Nutr. 125:947-955.
  2. Angel, C. R., J. L. Sell and D. R. Zimmerman. 1988. Autolysis of alpha-galactosides of defatted soy flakes: influence on nutritive value for chickens. J. Agric. Food Chem. 36:542-546. https://doi.org/10.1021/jf00081a034
  3. AOAC. 2005. Official methods of analysis. 16th edn. Association of Official Analytical Chemists, Washington, DC.
  4. Bach Knudsen, K. E. and B. W. Li. 1991. Determination of oligosaccharides in protein-rich feedstuffs by gas-liquid chromatography and high-performance liquid chromatography. J. Agric. Food Chem. 39:689-694. https://doi.org/10.1021/jf00004a013
  5. Brenes, A. R., R. Mareqardt, W. Guenter and B. A. Rotter. 1993a.Effect of enzyme supplementation on the nutritive value of raw, autoclaved and dehulled lupins (lupinus albus) in chickens diets. Poult. Sci. 72:2281-2293. https://doi.org/10.3382/ps.0722281
  6. Brenes, A., M. Smith, W. Guenter and R. R. Marquardt. 1993b.Effect of enzyme supplementation on the performance and digestive tract size of broiler chickens fed wheat and barley based diets. Poult. Sci. 72:1731-1739. https://doi.org/10.3382/ps.0721731
  7. Brouns, S. J. J., N. Smits, H. Wu, A. P. L. Snijders, P. C. T. Wrigh, W. M. de Vos and J. van der Oost. 2006. Identification of a novel $\alpha$-galactosidase from the hypertgermophilic archaeon Sulfolobus solfataricus. J. Bacteriol. 188:2392-2399. https://doi.org/10.1128/JB.188.7.2392-2399.2006
  8. Choct, M., R. J. Hughes, J. Wang, M. R. Bedford, A. J. Morgan,and G. Annison. 1995. Feed enzymes eliminate the antinutritive effect of non-starch polysaccharides and modify fermentation in broilers. Proc. Austr. Poul. Sci. Symp. 7:121-125.
  9. Coon, C., K. L. Leske, O. Akavanichan and T. K. Cheng. 1990.Effect of oligosaccharide free soya bean meal on true metabolizable energy and fiber digestion in adult roosters. Poult. Sci. 69:787-793. https://doi.org/10.3382/ps.0690787
  10. De Coca-Sompva, A., D. G. Valencia, E. J. Morrno, R. Lazara andG. G. Mateos. 2008. Apparent ileal digestibility of energy, nitrogen, and amino acids of soybean meals of different origin in broilers. Poult. Sci. 87:2613-2623. https://doi.org/10.3382/ps.2008-00182
  11. Duncan, D. B. 1955. Multiple range and mutiple F tests. Biometrics. 11:1-42. https://doi.org/10.2307/3001478
  12. Fleming, S. E. 1981. A study of relationships between flatus potential and carbohydrate distribution in legume seeds. J. Food Sci. 46:794-798. https://doi.org/10.1111/j.1365-2621.1981.tb15350.x
  13. Fuller, R. 1977. The importance of lactobacilli in maintaining normal microbial balance in the crop. Br. Poult. Sci. 18:89-94.
  14. Fuente, J. M., P. P. de Ayala and M. J. Villamide. 1995. Effect of dietary enzyme on metabolizable energy of diets with increasing levels of barley fed to broilers at different ages. Anim. Feed Sci. Technol. 56:45-53. https://doi.org/10.1016/0377-8401(95)00815-5
  15. Gdala, J., A. J. M. Jansman, L. Buraczewska, J. Huisman and P.van Leeuwen. 1997. The influence of $\alpha$-galactosidase supplementation on the ileal digestibility of lupin seed carbohydrates and dietary protein in young pigs. Anim. Feed Sci. Technol. 67:115-125. https://doi.org/10.1016/S0377-8401(97)00003-5
  16. Ghazi, S., J. A. Rooke, H. E. Galbraith and A. Morgan. 1997. Effect of adding protease and alpha-galactosidase enzymes to soyabean meal on nitrogen retention and true metabolizable energy in broilers. Br. Poult. Sci. 38(Suppl.):S28.
  17. Grieshop, C. M., C. T. Kadzere, G. M. Clapper, E. A. Flickinger, L.Bauer, R. L. Frazier and G. C. Fahey Jr. 2003. Chemical and nutritional characteristics of United States soybeans and soybean meals. J. Agric. Food Chem. 51:684-7691. https://doi.org/10.1021/jf034690c
  18. Guinotte, F., J. Gautron and Y. Nys. 1995. Calcium solubilization and retention in the gastrointestinal tract in chicks (Gallus domesticus) as a function of gastric acid secretion inhibition and of calcium carbonate particle size. Br. J. Nutr. 73:125-139. https://doi.org/10.1079/BJN19950014
  19. Hill, F. W., D. L. Anderson, R. Renner and L. B. Carew Jr. 1960. Studies on the metabolizable energy of grains and grain products for chickens. Poult. Sci. 39:573-579. https://doi.org/10.3382/ps.0390573
  20. Ikegami, S., F. Tsuchihashi, H. Harada, N. Tsuchihashi, E. Nishide, and S. Innami. 1990. Effect of viscous indigestible polysaccharides on pancreatic-billary secretion and digestive organs in rats. J. Nutr. 120:353-360.
  21. Irish, G. G., G. W. Barbour, H. L. Classen, R. T. Tyler and M. R.Bedford. 1995. Removal of the $\alpha$-galactosides of sucrose from soybean meal using either ethanol extraction or exogenous $\alpha$-galactosidase and broiler performance. Poult. Sci. 74:1484-1494. https://doi.org/10.3382/ps.0741484
  22. Jankowski, J., J. Juskiewicz, K. Gulewicz, A. Lecewicz, B. A.Slominski and Z. Zdunczyk. 2009. The effect of diets containing soybean meal, soybean protein concentrate, and soybean protein isolate of different oligosaccharide content on growth performance and gut function of young turkeys. Poult. Sci. 88:2132-2140. https://doi.org/10.3382/ps.2009-00066
  23. Kadim, I. T. and P. J. Moughan. 1997. Development of an ileal amino acid digestibility assay for the growing chicken-Effects of time after feeding and site of sampling. Br. Poult. Sci. 38:89-95. https://doi.org/10.1080/00071669708417946
  24. Kidd, M. T., G. W. Morgan, Jr., C. J. Price, P. A. Welch and E. A. Fontana. 2001a. Enzyme supplementation to corn and soybean meal diets for broilers. J. Appl. Poult. Res. 10:65-70. https://doi.org/10.1093/japr/10.1.65
  25. Kidd, M. T., G. W. Morgan, Jr. and C. D. Zumwalt. 2001b. $\alpha$-Galactosidase enzyme supplementation to corn and soybean meal broiler diets. J. Appl. Poult. Res. 10:186-193. https://doi.org/10.1093/japr/10.2.186
  26. Knap, I. H., A. Ohmann and N. Dale. 1996. Improved bioavailability of energy and growth performance from adding alpha-galactosidase (from Aspergillus sp.) to soybean meal based diets. In Proc. Aust. Poult. Sci. Symp. Sydney, Australia, pp. 153-156.
  27. Kotwal, S. M., M. M. Gote, S. R. Sainkar, M. I. Khan and J. M.Khire. 1998. Production of $\alpha$-galactosidase by thermophilic fungus Humicola sp. in solid-state fermentation and its application in soymilk by hydrolysis. Proc. Biochem. 33:337-343. https://doi.org/10.1016/S0032-9592(97)00084-8
  28. Leske, K. L., O. Akavanichan, T. K. Cheng and C. N. Coon. 1991.Effect of ethanol extract on nitrogen-corrected true metabolizable energy for soybean meal with broilers and roosters. Poult. Sci. 70:892-895. https://doi.org/10.3382/ps.0700892
  29. Leske, K. L., B. Zhang and C. N. Coon. 1995. The use of low $\alpha$-galactoside protein products as a protein source in chicken diets. Anim. Feed Sci. Technol. 54:275-286. https://doi.org/10.1016/0377-8401(95)00773-G
  30. Modler, H. W., R. C. McKellar and M. Yaguchi. 1990. Bifidobacteria and bifidogenic factors. Can. Inst. Food Sci. Technol. J. 23:29-41. https://doi.org/10.1016/S0315-5463(90)70197-6
  31. Mulimani, V. H. and S. T. Ramalingam. 1995. Enzymic hydrolysis of raffinose and stachyose present soymilk by crude $\alpha$-galactosidase from Gibberella fujikuroi. Biochem. Mol. Biol. Int. 36:897-905.
  32. Mulimani, V. H. and S. T. Ramalingam. 1997. Enzymatic degradation of oligosaccharides in soybean flours. Food Chem. 59:279-282. https://doi.org/10.1016/S0308-8146(96)00282-8
  33. Noy, Y. and D. Sklan. 2004. Effects of metabolizable energy and amino acid levels on turkey performance from hatch to marketing. J. Appl. Poult. Res. 3:241-252.
  34. Nowak, J. and K. H. Steinkraues. 1988. Effect of tempeh fermentation of peas on their potential flatulence productivity as measured by gas production and growth of clostridum perfringens. Nutr. Rep. Int. 38:1163-1171.
  35. d M. Araba. 2000. Nutritional evaluation of soybean meals varying in oligosaccharide content. Poult. Sci. 79:1127-1131. https://doi.org/10.1093/ps/79.8.1127
  36. Rotter, B. A., O. D. Friesen, W. Guenter and R. R. Marquardt.1990. Influence of enzyme supplementation on the bioavailable energy of barley. Poult. Sci. 69:1174-1181. https://doi.org/10.3382/ps.0691174
  37. Rubio, L. A., A. Brenes and M. Castano. 1990. The utilization of raw and autoclaved faba beans (Vicia faba L., var. minor) and faba bean fractions in diets for growing broiler chickens. Br. J. Nutr. 63:419-433. https://doi.org/10.1079/BJN19900130
  38. Salih, M. E., H. L. Classen and G. L.Campbell. 1991. Response of chickens fed on hull-less barley to dietary $\alpha$-glucanase at different ages. Anim. Feed Sci. Technol. 33:139-149. https://doi.org/10.1016/0377-8401(91)90052-T
  39. Saunders, D. R. and H. S. Wiggins. 1981. Conservation of mannitol, lactulose, and raffinose by the human colon. Am. J. Physiol. 241:G397-G402.
  40. SAS. SAS Institute Inc. 1996. SAS/STAT User’s Guide: Statistics. Release 8.0. SAS Institute Inc., Cary, North Carolina.
  41. Shivanna, B. D., M. Ramakrishna and C. S. Ramadoss. 1989. Enzymic hydrolysis of raffinose and stachyose in soymilk by $\alpha$-galactosidase from germinating guar (Cyamopsis tetragonolobus). Process Biochem. 24:197-201.
  42. Sibbald, I. R. and S. J. Slinger. 1962. The metabolizable energy of materials fed to growing chicks. Poult. Sci. 41:1612-1613. https://doi.org/10.3382/ps.0411612
  43. Turnbull, C. M., A. L. Baxter and S. K. Johnson. 2005. Waterbinding capacity and viscosity of Australian sweet lupin kernel fibre under in vitro conditions simulating the human upper gastrointestinal tract. Int. J. Food Sci. Nutr. 2:87-94.
  44. Viveros, A., A. Brenes, M. Pizzaro and M. Castano. 1994. Effect of enzyme supplementation of a diet based on barley, an autoclave treatment, on apparent digestibility, growth performance and gut morphology of broilers. Anim. Feed Sci. Technol. 48:237-251. https://doi.org/10.1016/0377-8401(94)90175-9
  45. Waldroup, P. W., C. A. Keen, F. Yan and K. Zhang. 2006. The effect of levels of $\alpha$-galactosidase enzyme on performance of Broilers fed diets based on corn and soybean meal. J. Appl. Poult. Res. 15:48-57. https://doi.org/10.1093/japr/15.1.48
  46. Wang, C. L., W. Q. Lu, D. F. Li and J. J. Xing. 2005. Effects of alpha-galactosidase supplementation to corn-soybean meal diets on nutrient utilization, performance, serum indices and organ weight in broilers. Asian-Aust. J. Anim. Sci. 18:1761-1768. https://doi.org/10.5713/ajas.2005.1761
  47. Zhang, L. Y., D. F. Li, S. Y. Qiao, J. T. Wang, L. Bai, Z. Y. Wangand In K. Han. 2001. The Effect of soybean galactooligosaccharides on nutrient and energy digestibility and digesta transit time in weanling piglets. Asian-Aust. J. Anim. Sci. 14:1598-1604. https://doi.org/10.5713/ajas.2001.1598

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