• Journal of Food Hygiene and Safety
• /
• v.31 no.4
• /
• pp.264-271
• /
• 2016

The purpose of this study was to investigate contamination sources of baby leafy vegetables by assessing microbial loads on baby leafy vegetables and agricultural inputs contacted with the vegetables. To estimate microbial loads, fecal indicators (coliform and Escherichia coli) and foodborne pathogens (E. coli O157:H7, Salmonella spp., Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus) were examined. A total of 126 samples including eleven kinds of leafy vegetables, irrigation water, media, and tools were tested, resulting in coliform contamination observed from most of samples. For E. coli, 10.3% (13/126) of the samples were positive including irrigation water, knife, handler, media, tools, and three kinds of leafy vegetables. B. cereus was detected from 38% (48/126) of the samples including media, tools and three kinds of leafy vegetables. No E. coli O157:H7, Salmonella spp., and L. monocytogenes was detected. This result implies that contacting with agricultural inputs could explain microbial load of baby leafy vegetables.

• Korean Journal of Soil Science and Fertilizer
• /
• v.43 no.1
• /
• pp.90-95
• /
• 2010

The application of livestock manure to cropland is a practice that has been used for centuries. Agricultural crops can utilize nutrients from manure, and the producer can utilize land for disposal, although in a "sustainable system" the concept is manure utilization and not waste disposal. However, meeting regulatory criteria regarding microbial quality remains an expensive and time consuming process. The purpose of this study was to quantify the level of environmental impact of soil moisture and temperature on fecal coliform (Escherichia coli or E. coli) survival in upland soils for sound application of livestock manure. Samples were collected up to 30 days depending on the given conditions. The inactivation rate of E. coli increased linearly with increased temperature while the inactivation rate gradually decreased with decreased soil moisture level. The overall findings of this study showed that the temperature was the limited factor on E. coli survival in soils over soil moisture content. This study will provide useful and practical guidelines to applicators of soil in deciding appropriate handling and time frames for land application for sustainable agriculture.

• Journal of Environmental Science International
• /
• v.10 no.5
• /
• pp.365-371
• /
• 2001

The optimum dosage of quicklime in producing organic fertilizer using livestock wastes vith a greater than 80% water content was analysed. After one day had elapsed to allow for the organic fentilizer to dry, the quicklime dosage and the composition of the organic fertilizer were analysed. Any from done to the organic fertilizer was also assessed. The amount of the quicklime required to stabilize livestock wastes was determined by water content of livestock wastes. For J farm(slurry style) of which livestock wastes have 94.6% of water concentration, less than 3% of total amount of livestock wastes, for H farm (scraper style) of which livestock wastes have 85% of water concentration, less then 4% of total livestock wastes and Y farm(traditional style) of which livestock wastes have 80% of water concentration, less then 5% of total livestock wastes. Generally, in order to pack the organic fertilizer, water containing quicklime0stabilized livestock wastes should be less than 35%. It takes 9 days to keep this water content for the wastes from H and Y farms(less than 85% in water content), and 12 days for the wastes from J (94.6% in water content). According to the classification standard for compost constitution by Higgins, the crude fertilizers from all 3 farms had high grade $K_2O$ and CaO, the middle grade T-N and middle or low grade $P_2O_5$. Stabilization by quicklime is known to inhibit bacterial decomposition of organic matter and the activity of pathogenic organisms. In this study, more then 99.99% of coliform group, fecal group and viable cell count were reduced. Our results indicate that livestock wastes of greater 80% water content could be used to produce organic fertilizer without the addition of a material for moisture control.

• Fisheries and Aquatic Sciences
• /
• v.17 no.2
• /
• pp.197-201
• /
• 2014

This study was performed to identify and assess food hazards during dry laver processing. Samples including raw materials, intermediates, and finished products during dried-laver processing were collected from seven dried-laver processing facilities, and microbial analyses were conducted. Microbial levels such as total coliforms and total viable cell count (TVC) increased as the processing steps progressed. TVC and total coliforms ranged from <30 to $9.1{\times}10^7$ CFU/g and <18 to 27,600 MPN/100 g for intermediates and finished products obtained during dried-laver processing, respectively. However, no fecal coliform was detected in the samples. Additionally, food-borne bacteria including Bacillus cereus, Listeria monocytogenes, Salmonella sp., Staphylococcus aureus, and Vibrio parahaemolyticus were not identified in finished products. For heavy metal content, arsenic ranged from 30.18 to 39.05 mg/kg, mercury from 0.005 to 0.009 mg/kg, and cadmium from 0.076 to 0.318 mg/kg dry mass in all finished products. However, lead was not detected in samples tested in this study. In conclusion, dried-laver products were safe based on the levels of food-borne bacteria and heavy metal contents. However, it is important to reduce total viable cell counts and total coliforms during dried-laver processing.

• Environmental Engineering Research
• /
• v.18 no.2
• /
• pp.95-101
• /
• 2013

Alternative sources of water are sought in some water stressed communities in the study area. The study focuses on the Aguata-Awka area of southeastern Nigeria. Aquifers occur at great depths, and surface waters may be far from homesteads. The scarcity of water has necessitated the people to adopt various local technologies for harvesting rainfall. The local technology includes collecting rainwater from roofs and channeling the water into large underground tanks, shallow wells and surface reservoirs. Large concrete tanks of $6m{\times}6m{\times}4m$ dimensions are often built underground and can store $144m^3$ of water. Surface reservoirs built on 4 m concrete pillar supports having dimensions of $10m{\times}10m{\times}4m$ and have a storage capacity of $400m^3$. Water samples were collected at 3 different locations of Agulu, Ekwulobia, and Awka and were analyzed for their physical, chemical, and bacteriological parameters. Results indicate a range of values for pH, 5.9 to 7.1; turbidity, 0.9 to 2.7; total dissolved solids, 80 to 170 mg/L; total hardness, 4.5 to 6.4 mg/L; magnesium, 1.2 to 1.4 mg/L; bicarbonate, 19.4 to 83.6 mg/L; and sulfate, 3.6 to 6.4 mg/L. Bacteriological analysis results were negative for fecal and total coliform counts. All parameters, with the exception of pH where aluminum and galvanized iron roofs are used for collection, fall within the recommended guidelines for drinking water quality of the World Health Organization, and the Standard Organization of Nigeria, new Nigerian standards for drinking water quality. Magnesium is above the maximum permitted level for consumer acceptability of the Nigerian standards for drinking water quality. The water can be classified as fresh moderately hard and soft. The water can be described as a calcium and bicarbonate type.

• Water for future
• /
• v.14 no.3
• /
• pp.55-66
• /
• 1981

The theory and practice of water quality scoring and indexing are introduced. The monthly water analysis data are available for six stations long the down-stream Han River whthin the areal boundary of the Special City of Seoul. The data cover the period between 1975 and 1979 inclusive and contain the analytical findings on 37 water constituents including DO, BOD, temperature, total solids and etc. Sic parameters are selected form the 37 items, that, to the judgement of the writer, best reflect the water quality of the Han River. They are; dissolved oxggen saturation, pH, fecal coliform, total solids, BOD and nitrate+ammonia. For each of the six parameters, a subscore function is developed and graphically presented to facilitate the transform of a measurment of the arameter to a subscore on a common score(e.G. 0-100) The score of a sample is calculated as a fuction of the six subscores, using four different approaches; (1) the unweighted arithmetic water quality score, (2) the weighted arithmetic water quality score, (3)the unweighted multiplicative score and (4) the reduced (total) score. Independent of these calculated scores, the experts' score which is calculated by averaging the ratings of water quality experts is obtained and compared with each of the four calculated scores by means of the least square method. The experts' score compares most favorably with the "reduced" score with the correlation coefficient of 0.956 : therefore this method of water quality scoring is adopted to calculate the Han River water quality scores and indices. Water quality index data for Guiri, ukdo, Pokwangdong, Noryangjin, Yongdungpo and Kayang Stations, 1975-1979 are as follow: The overall water quality index data of the Han River between Guiri and Kayang Stations are found; 47.3 in 1976, 48.0 in 1977, 48.5 in 1978 and 54.7 in 1979, indicating the general trend towards water quality improvent in this part of the river, in terms of the increased water quality index by average 1.85 points per year during this period. Finally the optimum sampling frequencies distributed among the six stations, using an equation which takes into account the coefficients of variation of the water quality scores and indices arec calculated.alculated.

• Journal of Animal Environmental Science
• /
• v.9 no.1
• /
• pp.45-56
• /
• 2003

$TAO^{TM}$ System is an auto-heated thermophilic aerated digestion process using a proprietary microbe called as a Phototropic Bacteria (PTB). High metabolic activity results in heat generation, which enables to produce a pathogen-free and digested liquid fertilizer at short retention times. TAO$^{TM}$ system has been developed to reduce a manure volume and convert into the liquid fertilizer using swine manure since 1992. About 100 units have been installed and operated in Korean swine farms so far. TAO$^{TM}$ system consists of a reactor vessel and ejector-type aeration pumps and foam removers. The swine slurry manure enters into vessel with PTB and is mixed and aerated. The process is operated at detention times from 2 to 4 days and temperature of 55 to $65^{\circ}C$. Foams are occurred and broken down by foam removers to evaporate water contents. Generally, at least 30% of water content is evaporated, 99% of volatile fatty acids caused an odor are removed and pathogen destruction is excellent with fecal coliform, rotavirus and salmonella below detection limits. The effluent from TAO$^{TM}$ system, called as the "TAO EFFLUX", is screened and has superb properties as a fertilizer. Normally N-P-K contents of screened TAO Efflux are 4.7 g/L, 0.375 g/L and 2.8 g/L respectively. The fertilizer effect of TAO EFFLUX compared to chemical fertilizer has been demonstrated and studied with various crops such as rice, potato, cabbage, pumpkin, green pepper, parsley, cucumber and apple. Generally it has better fertilizer effects and excellent soil fertility improvement effects. Moreover, the TAO EFFLUX is concentrated through membrane technology without fouling problems for a cost saving of long distance transportation and a commercialization (crop nutrient commodity) to a gardening market, for example.

• Korean Journal of Soil Science and Fertilizer
• /
• v.44 no.3
• /
• pp.368-370
• /
• 2011

Liquid manure fertilizer drived from pig slurry is a valuable source of nutrients for crop production. However, there is no study for environmental assessment regarding microbial quality to apply liquid manure fertilizer. Therefore, this study aimed at quantifying the level of environmental impact on fecal coliform (Escherichia coli or E. coli ) survival in saturated soil such as paddy field. Surface water samples were collected up to 168 and 11 hours under natural sunlight and artificial ultraviolet radiation, respectively. The inactivation rate of E. coli under natural sunlight increased gradually after 48 hours. However, the inactivation rate of E. coli under artificial ultraviolet radiation increased linearly over time. Our findings suggested that the ultraviolet radiation is the limited factor on E. coli survival in surface water of saturated soil. This result will provide useful and practical guideline to applicators of agricultural soil in deciding appropriate handling and time frames for preventing pollution of water quality for sustainable agriculture.

• Journal of Food Hygiene and Safety
• /
• v.13 no.3
• /
• pp.278-293
• /
• 1998

The purposes of this study were to develop Hazard Analysis Critical Control Point-based standardized recipe applicable to cook/chilled Broiled Spanish Mackerel in school foodservice operations and to establish reasonable shelf-life limits by assessing food quality during chilled storage period of 5 days. HACCP for the production of menu items was identified in simulation study. At each critical control point, time-temperature profile was recorded and microbiological analysis was done. Also chemical analyses and sensory evaluation were conducted for 5 days of chilled storage. The results of time-temperature measurement of Broiled Spanish Mackerel by each production phase showed satisfactory condition that met the standards. Broiled Spanish Mackerel showed excellent microbiological quality from raw ingredient phase ($TPC:2.58{\pm}0.12\;Log\;CFU/g$) to holding phase ($TPC:2.70{\pm}0.42\;Log\;CFU/g$). Coliform (0.84 Log MPN/g) and fecal coliform (0.84 Log MPN/g) were detected from marinating phase ($TPC:3.82{\pm}0.52\;Log\;CFU/g$). After heating, only few mesophiles were detected ($TPC:1.83{\pm}0.49\;Log\;CFU/g$). No psychrophiles, coliforms and fecal coliforms were detected. In the phases after rapid chilling, during chilled storage and after reheating and distribution, almost none of the above microbes were detected. Salmonella and Listeria monocytogenes were not detected in all production phases. The pH immediately after cooking was 6.65 and then increased significantly to 6.81 on the third day of chilled storage (p<0.001). Acid value did not show significant changes while total volatile based nitrogen (TVBN) dramatitically increased during storage periods (p<0.01). In the result of sensory evaluation, general acceptability points had been rated high in the first day of storage, and then, the points were decreased significantly on the third day (p<0.05). General acceptability points ranged from 8.86 to 10.68. Accordingly, Broiled Spanish Mackerel is highly recommendable cook/chill system. Considering the DHSS standards for storage, the ideal shelf-life recommended for Broiled Spanish Mackerel is within 4 days excluding cooking day. For Broiled Spanish Mackerel, critical control points were purchasing and receiving of frozen Spanish Mackerel, heating, chilling, chilled storage, reheating and distribution.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.19 no.2
• /
• pp.169-175
• /
• 1986

This study was carried out to evaluate the water quality of spring waters in Pusan area (see Fig. 1). In this experiment, one hundred and forty water samples were collected at 20 stations from July to December 1985. Range and mean value of constituents of the samples were as follows ; pH 6.2-8.2, 7.07 ; water temperature $4.0-23.5^{\circ}C,\;15.9^{\circ}C$ ; electrical conductivity $0.228{\times}10^{2}-2.125{\times}10^2{\mu}{\mho}/cm,\;0.860{\times}10^2{\mu}{\mho}/cm$; chloride ion 3.28-19.3mg/l, 6.81mg/l ; nitrite-nitrogen ND-0.221 mg/l, 0.017mg/l ; nitrate-nitrogen ND-6.779mg/l, 0.877 mg/l ; phosphate-phosphorus ND-0.105mg/l, 0.021mg/l ; silicate-silicious 2.12-22.70mg/l, 9.04mg/l, respectively. Especially, electrical conductivity, chloride ion, nitrite-nitrogen, nitrate-nitrogen, and silicate-silicious of the station 11 (Millakdong) were higher than those of others as $1.815{\times}10^2{\mu}{\mho}/cm$, 13.5mg/l, 0.076mg/l, 4.772mg/l and 14.07mg/l. Range and geometric mean value of total coliform and fecal coliform MPN's of the samples were 0-1,500/100ml, 13-470/100ml and 0-460/100ml, 2-32/100ml. Composition of coliform was $26.37\%$ Escherichia coli group, $21.98\%$ Citrobacter freundii group, $37.36%$ Entrobacter aerogenes group and $14.29\%$ others.