• 농업과학연구
• /
• 제47권3호
• /
• pp.437-443
• /
• 2020

The aim of this study was to evaluate the effect of Lactobacillus acidophilus probiotic (LAP) product supplementation on the blood profile, fecal noxious gas emission, and fecal shedding of lactic acid bacteria and coliform bacteria in healthy adult Beagle dogs. In total, 14 Beagle dogs with an average initial body weight of 10.19 ± 0.61 kg were randomly assigned into two dietary treatments,with and without LAP supplementation, for a 28-day feeding trial. At the end of the experiment, there was no significant (p > 0.05) difference in the concentration of serum total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), white blood cell (WBC), red blood cell (RBC), blood lymphocyte percentage, fecal hydrogen sulfide (H₂S) and total mercaptans (R.SH) emission, and fecal coliforms counts. However, the serum concentrations of the triglyceride and fecal ammonia (NH₃) emission of the LAP treatment were significantly (p < 0.05) decreased in the group compared with the CON dogs. Fecal total lactic acid bacteria counts were significantly (p < 0.05) increased in the LAP treatment. In conclusion, the supplementation of LAP in Beagle dog diets could decrease the blood triglyceride level and enhance the gut Lactobacillus count which may have positive effects on dogs.

• Journal of Veterinary Science
• /
• 제24권5호
• /
• pp.70.1-70.14
• /
• 2023

Background: Mycobacterium avium subspecies paratuberculosis (MAP) causes a chronic and progressive granulomatous enteritis and economic losses in dairy cattle in subclinical stages. Subclinical infection in cattle can be detected using serum MAP antibody enzyme-linked immunosorbent assay (ELISA) and fecal polymerase chain reaction (PCR) tests. Objectives: To investigate the differences in blood parameters, according to the detection of MAP using serum antibody ELISA and fecal PCR tests. Methods: We divided 33 subclinically infected adult cattle into three groups: seronegative and fecal-positive (SNFP, n = 5), seropositive and fecal-negative (SPFN, n = 10), and seropositive and fecal-positive (SPFP, n = 18). Hematological and serum biochemical analyses were performed. Results: Although the cows were clinically healthy without any manifestations, the SNFP and SPFP groups had higher platelet counts, mean platelet volumes, plateletcrit, lactate dehydrogenase levels, lactate levels, and calcium levels but lower mean corpuscular volume concentration than the SPFN group (p < 0.017). The red blood cell count, hematocrit, monocyte count, glucose level, and calprotectin level were different according to the detection method (p < 0.05). The SNFP and SPFP groups had higher red blood cell counts, hematocrit and calprotectin levels, but lower monocyte counts and glucose levels than the SPFN group, although there were no significant differences (p > 0.017). Conclusions: The cows with fecal-positive MAP status had different blood parameters from those with fecal-negative MAP status, although they were subclinically infected. These findings provide new insights into understanding the mechanism of MAP infection in subclinically infected cattle.

• Asian Pacific Journal of Cancer Prevention
• /
• 제17권sup3호
• /
• pp.17-22
• /
• 2016

Colorectal cancer is a major cause of mortality worldwide. Fecal occult blood testing has proven a very effective screening tool for early detection and mortality reduction. The aim of this study was to determine predictors factors related to fecal occult blood testing using the Health Belief Model method among residents of Bushehr, Iran. A cross sectional study was performed on a sample of 600 men and women more than 50 years of age. The sample was selected by a convenience method from patients referred to public and private laboratories throughout the city. Each subject filled out a questionnaire which was designed and developed based on Health Belief Model constructs. Statistical analysis was conducted using ANOVA, T-test, chi-square test, and logistic regression. Fecal occult blood tests were performed on 179 (29.8%) out of 600 subjects, of which 95 patients (58.1%) did a periodic examination test and 84 patients (46.9%) had a doctor's advice for testing. According to the logistic regression model, the perceived barriers (P=0.0, Exp(B)= 0.3), perceived benefits (P<0.01, Exp(B)= 1.9) and self-efficacy (P<0.01, Exp(B)= 1.6) were predictive factors related to occult blood testing among subjects. The results showed that reducing people's perception of barriers to testing, increasing perceived benefits of screening, and reinforcing self efficacy can have major effect in increasing the rate of fecal occult blood screening for colorectal cancer prevention.

• 대한한방소아과학회지
• /
• 제18권1호
• /
• pp.105-129
• /
• 2004

Methods: It was studied on the 47 kinds of oriental medical literature for fecal blood. Results: Fecal blood means bleeding with faces from anus and indicates all the three cases such as blooding before and after evacuation, evacuation of feces mixed with blood, and simple melena. The main causes are fire(火) and deficiency of spleen qi (脾氣虛). According to the color of fecal blood and the region of the bleeding, first, if the blood color is dark-red and blood discharges after emptying the bowels, it is called deposited poison into Jang(臟毒) or distant bleeding(遠血), and if the color of blood is clear and bowel emptying occurs after bleeding, it is bloody stool due to intestinal wind(腸風) or nearby bleeding(近血). For treating methods(治法). removing heat from the blood and stopping bleeding(淸熱凉血止血), removing dampness and stopping bleeding(淸熱除濕止血), invigorating Ki for promoting Hul-controJ(益氣攝血), warming the spleen and stomach to dispel cold and stopping bleeding(溫中散寒止血) etc are applied. As for the treating prescriptionl(治法), a Hwangtotang(黃土湯). Jeoksodudanggwitang(赤小豆當歸散). Gwihwasan(槐花散). Wipungtang(胃風湯). Hwangnyeonhwan modifying(黃連丸加減). Samultang modifying(四物湯加減). Paedoksan modifying(敗毒散加減) etc are used. As for acupuncture and moxibustion(鍼灸療法). if etiology(病因) is damp-heat(濕熱), acupuncture(刺鍼) at Janggang(長强); Charyo(BL 32); Sangeoheo(ST 37)(上巨虛); Seungsan(BL 57)(承山穴), and in case of deficiency of spleen Gi(脾氣虛), acupunture(刺鍼) with tonification(補法) at I Baek(EX-UE 2)(二白); Gwanwon(CC 4)(關元); Joksamni(ST 36)(足三里) Taebaek(SP 3)(太白); Hoeeum(CC 1)(會陰穴), or mxibuston(灸) at Baekoe(GC 2O)(百會); Myeongmun9GC (命門) or the point of opposite to umbilicus among spinal vertebrae(脊中對臍穴) are used. The external treatment(外治療法) was consisted of plastering umbilicus therapy(敷臍法) and enema therapy(灌腸法).

• Asian-Australasian Journal of Animal Sciences
• /
• 제33권4호
• /
• pp.597-604
• /
• 2020

Objective: This study was conducted to investigate the effect of zinc aspartic acid chelate (Zn-ASP) on growth performance, nutrient digestibility, blood profiles, fecal microbial and fecal gas emission in growing pigs. Methods: A total of 160 crossbred ([Landrace×Yorkshire]×Duroc) growing pigs with an initial body weight (BW) of 25.56±2.22 kg were used in a 6-wk trial. Pigs were randomly allocated into 1 of 4 treatments according to their sex and BW (8 replicates with 2 gilts and 3 barrows per replication pen). Treatments were as follows: i) CON, basal diet, ii) TRT1, CON+0.1% Zn-ASP, iii) TRT2, CON+0.2% Zn-ASP, and iv) TRT3, CON+0.3% Zn-ASP. Pens were assigned in a randomized complete block design to compensate for known position effects in the experimental facility. Results: In the current study, BW, average daily gain, and gain:feed ratio showed significant improvement as dietary Zn-ASP increased (p<0.05) in growing pigs. Apparent total tract digestibility (ATTD) of dry matter was increased linearly (p<0.05) in pigs fed with Zn-ASP diets. A linear effect (p<0.05) was detected for the Zn concentration in blood with the increasing levels of Zn-ASP supplementation. Lactic acid bacteria and coliform bacteria were affected linearly (p<0.05) in pigs fed with Zn-ASP diets. However, no significant differences were observed in the ATTD of nitrogen, energy and Zn. And dietary Zn-ASP supplementation did not affect fecal ammonia, hydrogen sulfide and total mercaptans emissions in growing pigs. Conclusion: In conclusion, dietary supplementation with Zn-ASP of diet exerted beneficial effects on the growth performance, nutrient digestibility, blood profiles and fecal microbes in growing pigs.

• 농업과학연구
• /
• 제49권3호
• /
• pp.531-537
• /
• 2022

This study evaluated the effects of dehulled lupin kernel (DLK) supplementation on growth performance, nutrient digestibility, blood urea nitrogen (BUN) and creatinine, fecal microbiota and fecal noxious gas emission in growing pigs. A total of 108 growing pigs (Landrace × Yorkshire × Duroc) with an average initial body weight (IBW) of 24.49 ± 3.2 kg were allocated to one of three dietary treatments (5, 10, and 20 % DLK) according to sex and BW in a randomized complete block design for 6 wk. Each dietary treatment consisted of 9 replication pens with 4 pigs per pen (2 gilts and 2 borrows). The results show that there were no significant differences in growth performance and nutrient digestibility among the treatments (p > 0.05). However, pigs fed the DLK20 diet had a higher BUN compared with those fed the DLK10 diet (p < 0.05). No effects were observed on the fecal microbiota and fecal noxious gas emission among the treatments. In conclusion, the inclusion of 20% DLK had no adverse effects on the performance in growing pigs.

• 농업과학연구
• /
• 제49권3호
• /
• pp.441-450
• /
• 2022

This study explored the effects of milk flavor (MF) supplementation on growth efficiency, nutrient absorption, fecal score, and blood profiles in weaning piglets. A total of 80 (21 days old) crossbred ([Yorkshire × Duroc] × Landrace) healthy weaned piglets with an initial body weight (BW) of 7.05 ± 1.22 kg were randomly allotted to one of two nutritive treatments with 8 repetitions and five pigs (2 female and 3 male) per pen. The experiment was divided into 2 phases (d 0 - 21, and d 21 - 42), and the dietary treatments consisted of TRT1, basal diet, TRT2 and basal diet + 1.0 g·kg^-1 MF. At days 21 - 42 and the overall period, the average daily gain (ADG) and average daily feed intake (ADFI) increased (p < 0.05) by receiving the MF added feed. However, MF inclusion did not impact (p > 0.05) the feed efficiency (G : F) throughout the entire experiment. Piglets consuming the MF supplemented diet showed that the apparent total tract digestibility of dry matter (DM), nitrogen (N) and energy (E) did not vary significantly (p > 0.05) between the treatments. All through the experiment, the fecal score and blood profile of the piglets fed the flavor diet also remained unaffected (p > 0.05). In conclusion, MF addition to the diet of the piglets increased their body weight and had no adverse effects on nutrient utilization, fecal score, and blood profile. Thus, MF addition could improve the performance outcomes of weaning piglets.

• Asian-Australasian Journal of Animal Sciences
• /
• 제25권10호
• /
• pp.1439-1444
• /
• 2012

A total of 144 pigs ((Landrace${\times}$Yorkshire)${\times}$Duroc)] with an average initial BW of $8.45{\pm}0.57$ kg were used in a 5-wk growth trial. Pigs were randomly allocated to 4 treatments with 9 replications per pen in a randomized complex block design. Dietary treatments included: i) CON (basal diet), ii) ANT (CON+tylosin 1 g/kg), iii) H1 (CON+H. cordata 1 g/kg) and iv) T1 (CON+T. officinale 1 g/kg). In this study, pigs fed the ANT and T1 treatment had a higher (p<0.05) average daily gain (ADG) and gain:feed (G:F) ratio than those fed CON and H1 treatment. Dietary ANT and T1 treatment led to a higher energy digestibility than the CON group. No difference (p>0.05) was observed on the growth performance and apparent total tract digestibility with H1 supplementation compared with the CON treatment. The inclusion of ANT treatment led to a higher (p<0.05) lymphocyte concentration compared with the CON treatment. Dietary supplementation of herbs did not affect (p>0.05) the blood characteristics (white blood cell (WBC), red blood cell (RBC), IgG, lymphocyte). No difference was observed on (p<0.05) fecal microbial shedding (E. coli and lactobacillus) between ANT and CON groups. Treatments H1 and T1 reduced the fecal E. coli concentration compared with the CON treatment, whereas the fecal lactobacillus concentration was not affected by the herb supplementation (p>0.05). In conclusion, the inclusion of T. officinale (1 g/kg) increased growth performance, feed efficiency, energy digestibility similarly to the antibiotic treatment. Dietary supplementation of T. officinale and H. cordata (1 g/kg) reduced the fecal E. coli concentration in weaning pigs.

• Asian-Australasian Journal of Animal Sciences
• /
• 제19권4호
• /
• pp.587-592
• /
• 2006

This study was conducted to evaluate the effects of supplementation with bacillus-based probiotic (Bacillus subtilis, $1.0{\times}10^7CFU/g$; Bacillus coagulans, $2.0{\times}10^6CFU/g$ and Lactobacillus acidophilus, $5.0{\times}10^6CFU/g$) on finishing pigs growth performance, nutrients digestibility, blood characteristics and fecal noxious gas content and to determine the optimal addition level of this probiotic preparation. A total of forty eight pigs with an initial body weight (BW) of $90.60{\pm}2.94kg$ were allotted to three dietary treatments (four pigs per pen with four pens per treatment) according to a randomized complete block design. Dietary treatment included: 1) CON (basal diet); 2) BP1 (basal diet+bacillus-based probiotic 0.1%) and 3) BP2 (basal diet+bacillus-based probiotic 0.2%). The experiment lasted 6 weeks. Through the entire experimental period, ADG was improved by 11% (p<0.05) in pigs fed diets supplemented with 0.2% bacillus-based probiotic compared to pigs fed the basal diet. ADFI and gain/feed were not affected by the treatments (p>0.05). Supplementation of bacillus-based probiotic did not affect either DM and N digestibilities or blood characteristics (p>0.05) of pigs. Fecal ammonia nitrogen ($NH_3$-N) measured at the end of experiment was reduced (p<0.05) when pigs were fed the diet with 0.2% bacillus-based probiotic. Fecal butyric acid concentration also decreased significantly (p<0.05) whereas acetic acid and propionic acid concentrations were not affected (p>0.05) when pigs were fed diets with added bacillus-based probiotic. In conclusion, dietary supplementation of bacillus-based probiotic can increase growth performance and decrease fecal noxious gas content concentration.

• Asian-Australasian Journal of Animal Sciences
• /
• 제18권10호
• /
• pp.1464-1468
• /
• 2005

The aim of this study was to assess the effects of dietary complex probiotic (Lactobacillus acidophilus, $1.0{\times}10^7$ CFU/g; Saccharomyces cerevisae, $4.3{\times}10^6$ CFU/g; Bacillus subtilis $2.0{\times}10^6$ CFU/g) on growth performance, nutrients digestibility, blood characteristics and fecal noxious gas content in growing pigs. Ninety [(Duroc${\times}$Yorkshire)${\times}$Landrace] pigs with the average initial BW of 39.75${\pm}$1.97 kg were allocated into three treatments by a randomized complete block design. There were five pens per treatment with six pigs per pen. Dietary treatments include: 1) CON (basal diet); 2) CP1 (basal diet+complex probiotic 0.1%) and 3) CP2 (basal diet+complex probiotic 0.2%). During the entire experimental period of 6 weeks, results showed that addition of complex probiotic at the level of 0.2% to diet increased ADG significantly (p<0.05). Also, digestibilities of DM and N tended to increase, however, no significant differences were observed (p>0.05). Blood characteristics (IgG, Albumin, total protein, RBC, WBC and lymphocyte) of pigs were not affected (p>0.05) by complex probiotic supplementation. Fecal $NH_3$-N was decreased (11.8%) significantly by the addition of complex probiotic (p<0.05), but no effects were observed on fecal acetic acid, propionic acid and butyric acid concentrations (p>0.05). In conclusion, results in this experiment indicated that dietary complex probiotic supplementation had a positive effect on growing pigs performance and could decrease fecal $NH_3$-N concentration.