• 제목/요약/키워드: Feathers

검색결과 144건 처리시간 0.026초

Production and Characterization of Keratinolytic Proteases by a Chicken Feather-Degrading Thermophilic Strain, Thermoactinomyces sp. YT06

  • Wang, Lin;Qian, Yuting;Cao, Yun;Huang, Ying;Chang, Zhizhou;Huang, Hongying
    • Journal of Microbiology and Biotechnology
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    • 제27권12호
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    • pp.2190-2198
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    • 2017
  • Thermoactinomyces sp. strain YT06 was isolated from poultry compost and observed to degrade integral chicken feathers completely at $60^{\circ}C$, resulting in the formation of 3.24 mg/ml of free amino acids from 50 ml of culture containing 10 g/l chicken feathers. Strain YT06 could grow and secrete keratinase using feather as the only carbon and nitrogen sources without other supplement, but complementation of 10 g/l sucrose and 4 g/l $NaNO_3$ increased the production of the keratinolytic enzyme. The maximum protease activity obtained was 110 U/ml and for keratinase was 42 U/ml. The keratinase maintained active status over a broad pH (pH 8-11) and temperature ($60-75^{\circ}C$). It was inhibited by serine protease inhibitors and most metal ions; however, it could be stimulated by $Mn^{2+}$ and the surfactant Tween-20. A reductive agent (${\beta}$-mercaptoethanol) was observed to cleave the disulfide bond of keratin and improve the access of the enzyme to the keratinaceous substrate. Zymogram analysis showed that strain YT06 primarily secreted keratinase with a molecular mass of approximately 35 kDa. The active band was assessed by MALDI-TOF mass spectrometry and was observed to be completely identical to an alkaline serine protease from Thermoactinomyces sp. Gus2-1. Thermoactinomyces sp. strain YT06 shows great potential as a novel candidate in enzymatic processing of hard-to-degrade proteins into high-value products, such as keratinous wastes.

생물분류탐구과정에서 호르몬 변화를 이용한 부정감성예측모델 개발 (Development of a Negative Emotion Prediction Model by Cortisol-Hormonal Change During the Biological Classification)

  • 박진선;이일선;이준기;권용주
    • 과학교육연구지
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    • 제34권2호
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    • pp.185-192
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    • 2010
  • 이 연구의 목적은 생물분류탐구과정에서 나타나는 부정감성을 호르몬 변화로 예측할 수 있는 부정감성 예측모델을 개발하는 것이다. 이를 위해 통합적인 과학 탐구가 가능하도록 깃털 분류 탐구 활동을 개발하였다. 연구대상은 호르몬 변화 측정에 문제가 없는 서울, 안산, 청주 소재 중학교 2학년 47명(남 18, 여 29)으로 하였다. 피험자들은 개인별로 깃털 분류 탐구 활동을 수행하였다. 깃털 분류 탐구 활동 전과 후에 형용사 이모티콘 척도법을 이용하여 부정감성 검사를 하였고, 타액 시료를 채취하여 코르티솔 호르몬 변화를 분석하였다. 연구결과 부정감성 변화량과 타액 코르티솔 변화량 사이에서 유의미한 정적 상관관계(R=0.39, P<0.001)가 나타났으며, 회귀분석을 이용하여 생물분류탐구에서 나타나는 타액 코르티솔 변화량을 이용한 부정감성 예측모델을 개발하였다.

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오훼골과 견갑골이 골절된 참매의 비침습적 재활 치료 성공 사례 (Noninvasive Treatment and Rehabilitation of a Northern Goshawk (Accipiter gentilis) with Coracoid and Scapular Fracture)

  • 김문정;김희종;김영준;박영석;김봉균;안병덕;박세영;이항
    • 한국임상수의학회지
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    • 제34권5호
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    • pp.396-399
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    • 2017
  • This is a case report of a northern goshawk (Accipiter gentilis), admitted to the wildlife rescue center with right coracoid and scapular fracture which received conservative treatment and was soft-released successfully. At the admission, the goshawk had callus formed on the fractured bones, scars on eyebrows and severely damaged tail feathers with inability to fly. Cage rest was indicated to prevent further occurrence of fracture and to promote reunion of the fracture surface. The bird went through a rehabilitation process with appropriate physical training in flying cage to recover its fitness and flying ability. Complete molting of flight feathers with damaged rectrices was achieved during the rehabilitation period and its flight ability was recovered favorably. It was released into a proper habitat for northern goshawks, but the bird was found near the aviary where its last phase of rehabilitation was performed. A soft-release program was applied and finally the bird returned to near the first rescue location 235 km away from the release site.

수용액 중 Orange II 흡착 제거를 위한 우모폐기물의 이용가능성 (Effectiveness of Feather Waste for Orange II Removal from Aqueous Solutions)

  • 박수연;유지연;손홍주
    • 한국환경과학회지
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    • 제27권9호
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    • pp.743-751
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    • 2018
  • The objective of this study was to investigate the adsorption potential of chicken feathers for the removal of OrangeII (AO7) from aqueous solutions. Batch experiments were performed as a function of different experimental parameters such as initial pH, reaction time, feather dose, initial OrangeII concentration and temperature. The highest OrangeII uptake was observed at pH 1.0. Most of the OrangeII was adsorbed at 2 h and an adsorption equilibrium was reached at 6 h. As the amount of chicken feather was increased, the removal efficiency of OrangeII increased up to 99%, but its uptake decreased. By increasing the initial concentration and temperature, OrangeII uptake was increased. The experimental adsorption isotherm exhibited a better fit with the Langmuir isotherm than with the Freundlich isotherm, and maximum adsorption capacity from the Langmuir constant was determined to be 0.179244 mmol/g at $30^{\circ}C$. The adsorption energy obtained from the Dubinin-Radushkevich model was 7.9 kJ/mol at $20^{\circ}C$ and $30^{\circ}C$ which indicates the predominance of physical adsorption. Thermodynamic parameters such as ${\Delta}G^0$, ${\Delta}H^0$, and ${\Delta}S^0$ were -12.28 kJ/mol, 20.64 kJ/mol and 112.32 J/mol K at $30^{\circ}C$, respectively. This indicates that the process of OrangeII adsorption by chicken feathers was spontaneous and endothermic. Our results suggest that as a low-cost biomaterials, chicken feather is an attractive candidate for OrangeII removal from aqueous solutions.

고구려인의 복식문화고찰-집안지역 고분벽화를 중심으로-

  • 양경애
    • 복식
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    • 제25권
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    • pp.183-199
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    • 1995
  • Among many different approaches of study of history of Kokuryo clothing culture, this study attempted to understand the costume by including political , militarial , economical , cultural , and religious aspects of Kokuryo society. It also included pictures in mural tumbs and old studies in Kokuryo costume. Clothing culture related to political life clearly differenciated social status by restricting styles and colors of clothes according to peoples social status. Generally people in high social status wore clothes with detailed decorations invariety of colors, and also used more fabrics than people in low social status. Costume was also affected by military life style that needed to meet the vigorous physical activities. High rank worriors wore iron scaled shielded clothes with red decoration on it which identified different ranks of the worriors. Economy also contributed it role to affect the clothing styles in Kokuryo. Based on old studies of costume, this study focused on people's life styles that included hunting and weaving . Hunting clothes were differentiated by decorating wit feathers . Jowoguan(조우관) is one of the examples. Since weaving was a major economic power or factor, silk and flax were very popular. People even used those fabrics to pay tax. Regarding cultural aspect , this study emphasized on song and dance wear along with active wear. Song and dance wear specially decorated with poka dots, however men and women wear were claryly distinguished. Unlike other clothes that were restricted in colors and styles, song and dance wear were decorated in variety of colors. Lastly this study presents religious aspects of Kokuryo costume . Monks wore black clothing (내의) that was influenced from China and the Western Regions(서역). To emphasize their religious power, Gods in the pictures of mural tumbs wore clothes with wings and feathers. God of fire and agriculture wore simple dresses without much decorations which represented simple life style of working class.

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고구려인의 복식문화 고찰

  • 양경애
    • 복식
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    • 제25권
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    • pp.185-199
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    • 1995
  • Among many different approches of study of history of Kokuryo clothing culture, this study attempted to understand the costume by in-cluding political, militarial, economical, cul-tural, and religious aspects of Kokuryo society. It also included pictures in mural tumbs and old studies in Kokuryo costume. Clothing culture related to political life clearly differenciated social status by restricting styles and colors of clothes accord-ing to peoples social status. Generally people in high social status wore clothes with detailed decorations in variety of colors, and also used more fabrics than people in low social status. Costume was also affected by military life style that needed to meet the vigorous physi-cal activities. High rank worriors wore iron scaled shielded clothes with red decoration on it which identified different ranks of the worriors. Economy also contributed its role to affect the clothing styles in Kokuryo. Baseed on old studies of costume, this study focused on people's life styles that included hunting and weaving. Hunting clothes were differentiated by decorating with feathers. Jowoguan is one of the exemples. Since weaving was a major economic power or factor, silk and flax were very popular. People even used those fabrics to pay tax. Regarding cultural aspect, this study emphasized on song and dance wear along with active wear. Song and dance wear specially decorated with poka dots, however means and womens wear were claryly dis-tinguished. Unlike other clothes that were re-stricted in colors and styles, song and dance wear were decorated in variety of colors. Lastly this study presents religious aspects of Kokuryo costume. Monks wore black cloth-ing that was influenced from China and the Western Regions. To emphasize their religious power. Gods in the pictures of mural tumbs wore clothes with wings and feathers. God of five and agriculture wore simple dresses without much decorations which represented simple life style of working class.

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대본청 앵무(Psittacula eupatria )로부터 PCR에 의한 avian polyomavirus 최초 검출 (First detection of avian polyomavirus by PCR from Alexandrine Parakeet (Psittacula eupatria) in Korea)

  • 김희정;이선락;박최규
    • 한국동물위생학회지
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    • 제37권3호
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    • pp.213-218
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    • 2014
  • In early April 2014, a month-old Alexandrine Paraqeet (Psittacula eupatria) that was raised in a domestic aviary located in Gyungju-si, Korea was suddenly died and submitted to Animal Disease Intervention Center, Kyungpook National University in order to diagnose the causative agent. In post-mortem examination, the bird had abnormally developed feathers on the neck and abdomen region and subcutaneous hemorrhages on the neck and cheek adjacent to the beak. At necropsy, the bird had hemorrhage on the muscle of the femoral region, ascites, multi-focal hemorrhages on the epicardium, and diffuse hemorrhages on the sub-serosa of proventriculus and gizzard, suggesting typical avian polyomavirus (APV) infection. The partial large tumor (T) antigen gene of APV was detected by PCR from tissues of the heart, lung, liver, kidney, proventriculus and feathers of the APV-suspected birds. However, other pathogenic virus-specific nucleic acid common with psittacine birds such as avian bornavirus, psittacine beak and feather disease virus and psittacid herpesvirus were not detected from the mixed tissue samples of the bird, indicating this case is due to single infection of APV. Nucleotide sequence analysis of the partially amplified large T antigen DNA was confirmed to have 99~100% homology with that of the previously reported APV strains. This case report describes the first detection of APV in Alexandrine Paraqeet in Korea.

동(東)아프리카 지역(地域)에서 광범위(廣範圍)하게 착용(着用)하는 Kanga개념(槪念) 연구(硏究) (A Study on Kanga Fundanental Notion of Apparel Widely Throughout East Africa)

  • 강은숙
    • 패션비즈니스
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    • 제8권4호
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    • pp.104-116
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    • 2004
  • Kanga is a type of cotton clothes with splendid patterns that East African women throw on their bodies. It first appeared in the East African shores in the mid-nineteenth century. A new style of squared handkerchiefs brought to Africa by Portuguese merchants for the first time was referred as to leso of which early designs were in a basic form of white dots on dark background. Consumers called such material by kanga as they began mentioning its craftiness and comparing its elegant nature to a sociable red rooster and graceful feathers. From the early 1990s, Swahili characters have been embroidered in designs of kanga, mainly consisted of proverbs. Kenya's kanga products are widely known and well represented whereas Tanzania makes the best use of it for political and social events. Fascinating and practical kanga has established its position as an essential part of East African cultures that is being well received as a fashion style there in these days.

고고관(姑姑冠)에 관한 연구 - "집사(集史)"를 중심으로 - (A Study on the Go-go-kwan(姑姑冠) -With a Special Reference to "Jip-sa(集史)"-)

  • 김미자
    • 복식
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    • 제58권3호
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    • pp.105-115
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    • 2008
  • A Go-go-kwan is a particular hat which has worn by married woman in Mongol and Yuan dynasty in China. It is also widely called as a Boktak in Mongol. The Go-go-kwan, recorded in jip-sa and in the miniature painting of 14th century is the early type of go-go-kwan. Later type can be seen from the relic collections of Gyeonggi Provincial Museum in Korea. The Go-go-kwan is composed with three parts ; feather part, tube part and hat part. Among the feathers, the largest one is as long as four times of the length of the tube and the shortest one is half the length of the tube. The tube is made of light weight wood such as white birch or bamboo, and covered with silk. Some of the hats were made exactly to fit on the head whereas others were designed to wear on the head as like jobawi.

Synergistic Effect of Reductase and Keratinase for Facile Synthesis of Protein-Coated Gold Nanoparticles

  • Gupta, Sonali;Singh, Surinder P.;Singh, Rajni
    • Journal of Microbiology and Biotechnology
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    • 제25권5호
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    • pp.612-619
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    • 2015
  • We have synthesized gold nanoparticles (GNPs) using chicken feathers (poultry waste) and Bacillus subtilis RSE163. Disulfide reductase and keratinase produced by Bacillus subtilis during the degradation of chicken feather has been used to reduce Au3+ from HAuCl4 precursor to produce gold nanoparticles. The synthesized biogenic GNPs were characterized by UV-visible spectroscopy, transmission electron microscopy (TEM), and zeta potential measurements. Fourier transform infrared (FTIR) spectroscopy indicated the presence of protein capping on synthesized GNPs, imparting multifunctionality to the GNP surface. Furthermore, the nontoxic nature of biogenic GNPs was insured by interaction with Escherichia coli (ATCC11103), where TEM images and enhancement of growth rate of E. coli in log phase signified their nontoxic nature. The results indicate that the synthesis of biocompatible GNPs using poultry waste may find potential applications in drug delivery and sensing.