• Journal of Microbiology
• /
• 제41권2호
• /
• pp.89-94
• /
• 2003

Bacterial strain No. P08 isolated from wastewater at the Cheongju industrial complex was found to be capable of degrading 4-chlorobenzoate under aerobic condition. P08 was identified as Comamonas sp. from its cellular fatty acid composition and 16S rDNA sequence. The fcb genes, responsible for the hydrolytic dechlorination of 4-chlorobenzoate, were cloned from the plasmid pJJl of Comamonas sp. P08. The fcb gene cluster of comamonas sp. PO8 was organized in the order fcbB-fcbA-fcbTl-fcbT2-fcbT3-fcbC. This organization of the fcb genes was very similar to that of the fcb genes carried on the chromosomal DNA of pseudomonas sp. DJ-12. However, it differed from the fcbA-fcbB -fcbC ordering of Arthrobacter sp. SU. The nucleotide sequences of the fcbABC genes of strain P08 showed 98% and 53% identities to those of Pseudomonas sp. DJ-12 and Arthrobacter sp. SU, respectively. This suggests that the fcb genes might have been derived from Pseudomonas sp. DJ-12 to form plasmid pJSl in Comamonas sp. P08, or that the fcb genes in strain DJ-12 were transposed from Comamonas sp. P08 plasmid.

• Journal of Microbiology and Biotechnology
• /
• 제8권6호
• /
• pp.692-695
• /
• 1998

Pseudomonas sp. DJ-12 was able to degrade 4-chlorobenzoate by hydrolytic dechlorination to produce 4-hydroxybenzoate and chloride ion. The fcbABC genes responsible for the hydrolytic dechlorination were cloned from the chromosomal DNA of the organism. The genes were found to be organized in the order fcbB-fcbA-fcbC, but there was an intergenic space between the fcbA and fcbC genes.

• 한국초전도학회:학술대회논문집
• /
• 한국초전도학회 2006년도 High Temperature Superconductivity Vol.XVI
• /
• pp.37-37
• /
• 2006

• 한국초전도학회:학술대회논문집
• /
• 한국초전도학회 2006년도 High Temperature Superconductivity Vol.XVI
• /
• pp.97-97
• /
• 2006

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제5권6호
• /
• pp.449-455
• /
• 2000

Pseudomonas sp. strain DJ-12 is a bacterial isolate capable of degrading 4-chlorobiphenyl (4CBP) as a carbon and energy source. The catabolic degradation of 4CBP by the strain DJ-12 was studied along with the genetic organization of the genes responsible for the crucial steps of the catabolic degradation. The catabolic pathway was characterized as being conducted by consecutive reactions of the meta-cleavage of 4CBP, hydrolytic dechlorination of 4-chlorobenzoate (4CBA), hydroxylation of 4-hydroxybenzoate, and meta-cleavage of protocatechuate. The pcbC gene responsible for the meta-cleavage of 4CBP only showed a 30 to 40% homology in its deduced amino acid sequence compared to those of the corresponding genes from other strains. The amino acid sequence of 4CBA-CoA dechlorinase showed an 86% homology with that of Pseudomonas sp. CBS3, yet only a 50% homology with that of Arthrobacter spp. However, the fcb genes for the hydrolytic dechlorination of 4CBA in Pseudomonas sp. DJ-12 showed an uniquely different organization from those of CBS3 and other reported strains. Accordingly, these results indicate that strain DJ-12 can degrade 4CBA completely via meta-cleavage and hydrolytic dechlorination using enzymes that are uniquely different in their amino acid sequences from those of other bacterial strains with the same degradation activities.

• 한국식품과학회지
• /
• 제46권5호
• /
• pp.660-664
• /
• 2014

본 연구는 최근 매우 우수한 가열 매체로 인정받고 있는 저온스팀(soft steam) 열처리 공정의 natural convection (NC) 방식과 forced convection-boiler (FC-b) 및 forced convection-fan (FC-f) 방식을 이용하여 처리온도(60, 70, $80^{\circ}C$) 및 시간(0-60분)에 따른 감자의 전처리 특성을 비교 평가하고자 하였다. 스팀처리 방식에 따른 가열실 내부의 온도변화와 감자 내 외부의 온도변화, 감자의 cook value, ascorbic aicd 및 수분의 함량과 중량감소율의 변화를 관찰한 결과, 모든 처리조건에서 FC-b 및 FC-f의 강제대류 방식에 비해 자연대류의 NC 방식이 이들 전처리 특성에 대해 우수한 결과를 나타냄을 확인할 수 있었다. 따라서, 본 연구의 결과들은 감자의 가공 및 전처리를 위해 현재 사용되고 있는 기존의 열처리 공정에 대한 대안으로써 NC 방식 스팀처리의 높은 효율성 및 산업적 적용에 대한 가능성을 제시하고 있다.