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Structural Analysis of the fcbABC Gene Cluster Responsible for Hydrolytic Dechlorination of 4-Chlorobenzoate from pJS1 Plasmid of Comamonas sp. P08  

Lee, Jeong-Soon (Department of Microbiology and Biotechnology, Chungbuk National University)
Lee, Kyoung (Department of Microbiology, Changwon National University)
Ka, Jong-Ok (Department of Agricultural Biology, Seoul National University)
Jong-Chan (Biotechnology Center Agriculture and Environment Rutgers University)
Kim, Chi-Kyung (Department of Microbiology and Biotechnology, Chungbuk National University)
Publication Information
Journal of Microbiology / v.41, no.2, 2003 , pp. 89-94 More about this Journal
Abstract
Bacterial strain No. P08 isolated from wastewater at the Cheongju industrial complex was found to be capable of degrading 4-chlorobenzoate under aerobic condition. P08 was identified as Comamonas sp. from its cellular fatty acid composition and 16S rDNA sequence. The fcb genes, responsible for the hydrolytic dechlorination of 4-chlorobenzoate, were cloned from the plasmid pJJl of Comamonas sp. P08. The fcb gene cluster of comamonas sp. PO8 was organized in the order fcbB-fcbA-fcbTl-fcbT2-fcbT3-fcbC. This organization of the fcb genes was very similar to that of the fcb genes carried on the chromosomal DNA of pseudomonas sp. DJ-12. However, it differed from the fcbA-fcbB -fcbC ordering of Arthrobacter sp. SU. The nucleotide sequences of the fcbABC genes of strain P08 showed 98% and 53% identities to those of Pseudomonas sp. DJ-12 and Arthrobacter sp. SU, respectively. This suggests that the fcb genes might have been derived from Pseudomonas sp. DJ-12 to form plasmid pJSl in Comamonas sp. P08, or that the fcb genes in strain DJ-12 were transposed from Comamonas sp. P08 plasmid.
Keywords
fcb genes; pJS1 plasmid; hydrolytic dechlorination; 4-chlorobenzoate; Comamonas sp. P08;
Citations & Related Records
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