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Characteristics of Persistent of Hexachlorocyclohexane(HCH) in Ambient Air-Soil-Water-Sediment for a Emerging Persistent Organic Pollutants(POPs) (신규 잔류성 유기오염물질(Emerging Persistent Organic Pollutants E-POPs) 핵사클로로시클로핵산(HCH)의 대기-토양-물-저질에서의 잔류특성)

  • Hwang, Sung-Min;Lee, Seok-Hyung;Park, No-Jin;Ok, Gon
    • Journal of Environmental Science International
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    • v.19 no.12
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    • pp.1343-1354
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    • 2010
  • The purpose of the study was to analyze the persistence of HCH in atmosphere, soil, sediment and water of the western and southern regions of Korea. The samples from the western region were collected from Anmyeon Island, and the samples from the southern region were collected from Kimhae and Busan. The concentration of HCH isomers in atmosphere showed the pattern of $\alpha$-HCH>$\gamma$-HCH>$\beta$-HCH. The regions with high HCH concentration in the atmosphere are the regions that have been highly exposed to HCH used in the past, and the areas that have been influenced by the long range transport. The HCH that persists in the soil, water and sediment evaporates into the atmosphere, showing the characteristics of Air-Surface exchange. When the regional concentration distributions are compared, the concentration of HCH was higher in the atmosphere of a plain and the cities near the plain, than the urban areas. In this study, the ratio of $\alpha/\beta$-HCH was used as an indicator for estimating the source of Technical HCH and Lindane. According to the result, the contribution of Lindane was high in Kimhae plain and Kimhae urban areas. However, in Busan, the contribution of Technical HCH was higher than Lindane. In case of Anmyeon Island, the western region of Korea had high contribution from Tehcnical HCH. In soil and sediment, $\beta$-HCH was dominant. In water, $\gamma$-HCH was dominant among other isomers. Such results are due to $\gamma$-HCH inLindane. Furthermore, the source of $\gamma$-HCH in urban areas is assumed to be the use of medicine, medical supplies and other living supplies. Based on the results of this study, the management of HCH, a newly list up emerging POPs, should be strengthened by further research on sources, fate, persistency, accumulation and exposures and etc. to the risk assessments.

A Study on the Strengths Model-based Parenting Experience of the Children with ADHD (강점관점으로 본 ADHD 아동 어머니의 양육 경험에 관한 연구)

  • Choi, Mal-Ok
    • Korean Journal of Social Welfare
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    • v.64 no.1
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    • pp.325-355
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    • 2012
  • This study was aimed to seek the essential meaning of the Strengths Model based experience of the mothers of the children with ADHD. To achieve such goal, a phenomenological study was carried out from April 2010 to April 2011 covering about 12 months in the way of 2~3 times of interviewing with 14 mothers of the children with ADHD. As a result, the essence of the experiences of those mothers was interpreted as 'the running of unwanted marathon struggling themselves to complete it'. The joining in the unwanted marathon turned out to have led 'me' the mother of the children with ADHD into miserable pain, but that is the 'energy' for 'my child' to endure the world. The essence of those mothers' experiences were divided into eight processes by timely constitution. They firstly experience 'a serial confusion' by their children's odd behaviour which turns out to be 'a semiconscious feeling by the diagnosis with 'ADHD' but then they make full efforts for their children accepting their 'maternal fate'. In that process, they participate in the unwanted marathon with the feeling of misery and gratitude for the 'sayings of teachers which play the roles of shot and grace'. The process was expressed as 'marathon like pain'. They make a resolve to live positively in the midst of lonely struggle unable to give up toward the finish line for 'my child and myself'. They encourage themselves for their children, but that's not easy process. Nevertheless, they gain enduring energy supported by their child, family and specialists. Those mothers keep running today also for their 'beloved doves enduring the ongoing hardship' from the raising of abnormal child toward the finish line of the marathon they have to run for even though the 'finish line' may turn into' 'starting point' again'. This researcher submits here the maternal experiences above as the task to practice social welfare for the mothers of the children with ADHD and the children themselves.

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Multivesicular bodies 및 Cytolysomes에 관(關)한 전자현미경적(電子顯微鏡的) 연구(硏究)

  • Kim, Woo-Kap;Kim, Chang-Whan;Park, Hong-Duok;Yang, He-Young
    • Applied Microscopy
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    • v.6 no.1
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    • pp.21-32
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    • 1976
  • The origins and the functions of the multi vesicular bodies and the various structures of the membranes related to the cytolysomes were studied in the mycelium cells of Rhizopus nigricans, Aspergillus niger and A. ochraceus, in the hymenium and basidium cells of Agricus bisporus sand Rhizopogon rubesecens, in the cells of assimilation tissue of Marchtantia polymorpha and Pogonalum inflexum and in the mesophyll cells of Pteridium aqiulinum, Pinus densiflora, Ginkgo biloba and Panax ginseng fixed with glutaraldehyde-paraformaldehyde-$ OsO_4$. In Rhizopus nigricans, Aspergillus niger, A. ochraceus, Agricus bisporus sand Rhizopogon rubescens, the concentric multilamellar, multivesicular, myelin-vesicle-tubular and concentric parallel-lamellar complexes were originated from the plasmalemma, while in Marehantia polymorpha, Pogonatum inflexum, Pteridium aquilinum, Pinus densiflora, Ginkgo biloba and Panax ginseng, they were originated from plasmalemma and the cytoplasm. The structures originated from the plasmalemma may be grouped into multi vesicular body and myelin-like structure, both forming the secondary vacuoles or protruding into the central vacuoles and finally degrading, In some cases, endoplasmic reticulum within the cytoplasm encloses some part of the cytoplasm to form a circle where the membranous lamellae increase in number, while the enclosed cytoplasm decrease to be eventually replaced by the multilamellar structure which is released into the vacuoles and subsquently degraded. The structures originated from the cytoplasm are believed to be the cytosegresomes or cytolysomes closely related to the differentiation of the vacuoles. The possible fate of these structures are also discussed.

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Altering of Collagens in Early Pregnant Mouse Uterus (착상전 생쥐 자궁에서 콜라겐의 변화)

  • Cheon, Yong-Pil
    • Development and Reproduction
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    • v.11 no.1
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    • pp.1-11
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    • 2007
  • Specific endometrial preparation should occur during periimplantation period. That is a progress of serial differentiation and is absolute in implantation of embryo and successful pregnancy. Remodeling of tissues shown during embryogenesis is regulated by various factors including extracellular matrix (ECM). Marked changes during pregnancy are including embryo migration, decidual response, and differentiation of placenta in placental animals including human. These changes to successful implantation in embryo and uterus have to prepare the competence for attachment of embryo and uterus, and invasion defense of uterus. During these changes, ECM dramatically changes for maintaining the uterine and embryonic functions. The major component of most connective tissue is collagens. It is very complex and hard to explore the mechanisms for ECM modulation. Recently using high throughput methodology, PCR-select cDNA subtraction method, microarray, many candidate genes have been identified. Steroid hormones have fundamental role in implantation and maintenance of pregnancy. Dermatopontin, a regulator of collagen accumulation, is regulated spatio-temporally in the uterus by primarily progesterone through progesterone receptors at the time of implantation. Modulation of extracellular matrix is critically regulated by cascade of gene net-works which are regulated by cascade of sex steroid hormones. Pathological regulation of uterine extracellular matrix reported in diabetic patients. To know the extracellular modulation is essential to understanding implantation, feto-placental development and overcome the paths involved in female reproduction. Though ECM composed with very various components and it is complex, the present review focused on the fate of collagens during periimplantation period.

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Development of nuclear Transfer Embryos using Somatic Cell Nuclei from Korean Native Cattle (Hanwoo) with High Genetic Value (고능력 한우 종모우 체세포를 이용한 핵이식 배아의 발달에 관한 연구)

  • Park, S. W.;M. R. Shin.;Kim, Y. H. .;H Shim;Kim, N. H.
    • Korean Journal of Animal Reproduction
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    • v.25 no.1
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    • pp.51-61
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    • 2001
  • This study was conducted to determine developmental ability of reconstructed embryos by nuclear transfer using somatic cell of Korean bull with high genetic value. Fibroblast cells obtained from ear biopsy of the bull were cultured in Dulbecco's Modified Eagle's medium (DMEM) at 37$^{\circ}C$ in air containing 5% $CO_2$. The cummulus-oocyte complexes were collected from slaughterhouse and were matured in vitro for 20 h in TCM 199 culture medium and the oocytes were then enucleated in modified phosphate buffered saline with cytochalasin B. Matured bovine oocytes were enucleated by aspirating the first polar body and metaphase chromatin using a beveled pipette in modified phosphate buffered saline. The ear fibroblast cells were fused into enucleated oocyte by electrical stimulation. The reconstructed oocytes were activated with ionomycine and 6-dimethylaminopurine, and then cultured in CR1aa medium for 7.5 days. Out of 524 bovine eggs reconstructed by nuclear transfer 65.6%(277/422) embryos were cleaved, and 30.7% (85/277) cleaved embryos were developed to the morula to blastocysts. There was no difference of developmental ability in vitro of reconstructed embryos regardless of donor cell passages. In order to determine fate of foreign mitochondria of donor nucleus, the Mito Tracker stained cells were fused into enucleated oocytes. The donor mitochondria were detected early stage of embryos, but disappeared rapidly. The developmental ability of reconstructed embryos was not impaired by Mito Tracker treatments. The results indicate that viable reconstructed embryos can be producted by nuclear transfer using somatic cell of Korean bulls.bulls.

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Efficacy of Supplemental Microbial Phytase on Laying Performance and Phosphorus Utilization II. Effect of Microbial Phytase at Different Phosphorus Levels and High Calcium Content on Laying Performance and Phosphorus Utilization (산란생산성과 인 이용성에 대한 Microbial Phytase의 첨가 효과 II. 무기태인 수주닝 다르고 칼슘수준이 높은 사료에 Microbial Phytase 첨가가 산란성 및 인 이용성에 미치는 영향)

  • 김상호;유동조;이상진;강보석;서옥석;최철환;이원준;류경선
    • Korean Journal of Poultry Science
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    • v.27 no.1
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    • pp.25-30
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    • 2000
  • Present study was conducted to investigate effects of microbial phytase in laying hen diets on utilization of non-phytate phosphorus(NPP) whose levels were adjusted to be adequate or lower than that of NRC requirements. Birds of control roup were fed a diet containing 0.275% NPP and 3.4% Ca, satisfying the NRC(1994) feeding standard. bird on T1, T2 and T3 were allowed to eat diets containing NPP at 100, 80 and 60%, respectively, of Control group, and 4.0% Ca level along with a microbial phytase added at a level of 300 DPU. Three hundred and sixty, ISA Brown layers, 23-week-old, divided into four treatment groups with three replications per treatment and 30 layers per replication were fed the diets for 12 weeks. Levels of feed intake were not different among the groups, The egg mass/feed intake ratio appeared better in T2 group by about 8%, though without a statistical significance, compared to that of control. Egg production fate tended to be improved over the control group by feeding the 100%(T1) and 80%(T2) NPP diets added with phytase, with a significant difference for T2(p<0.05). Mean egg weight and egg shell quality, measured by breaking strength and thickness of the egg shell, of the T2 group tended to show numerically better, without a significance than those of control. Furthermore, birds of the T2 group showed higher calcium and phosphorus contents in tibia by about 9%(p<0.05) than the control. Overall performances of birds in T1 appeared better than those of control, but tended to be lower than those of the T2. The birds in T3 performed similar to the those of the other dietary groups except the relatively low tibia calcium level. In conclusion, the results of this study suggest that supplementation of microbial phytase at a level of 300 DPU was effective to spare about 20% of NPP in laying hen diets without any adverse effects on production performances and bone quality.

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Preliminary study on colloidal partitioning and speciation of trace metals in acid mine drainage

  • Kwon, Jang-Soon;Lee, Jeong-Ho;Yun, Seong-Taek;Jung, Hun-Bok;Chang, Min-Kyoung;Lee, Pyeong-Ku
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2004.04a
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    • pp.100-101
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    • 2004
  • Many researches in Korea have been performed to understand the pollution of stream waters by acid mine drainage. However, few studies have been conducted regarding the effect of particulate and colloidal fractions on the transport of trace metals. To estimate harmful effects of trace metals, it is important to evaluate the particulate and colloidal metals as well as dissolved metals, because particulate and colloidal fractions of trace metals play an important role in transport of trace metals and may adversely affect habitats and organisms in riverine system. Colloids are solids with effective diameters in size range from 0.001 $\mu$m to 1 $\mu$m. According to Jone et al. (1974), metals in surface water, like Al, Fe, and Mn, require filtration with pore-size membranes smaller than 0.45 $\mu$m to define dissolved concentrations. The main objective of this study is to understand the effects of particulate, colloidal, and truly dissolved fractions on the transport and fate of trace metals in acid mine drainage. This study was conducted for the Onjeong creek in the Uljin mine area. Sampling was carried out in 13 sites, spatially covering the area from mine dumps to the downstream Onjeong reservoir. To examine the metal partitioning between particulate, colloidal, and truly dissolved fraction, we used successive filtration techniques consisting of conventional method (using 0.45 $\mu$m membranes) and tangential-flow ultrafiltration (using 0.001 $\mu$mm membranes). Ultrafiltration may seperate much smaller particles from aqueous phase (Josephson, 1984; Hernandez and Stallard, 1988). The analysis of metals were performed by inductively coupled plasma - atomic emission spectrometer (ICP-AES: model Perkin Elmer OPTIMA3000XL). Anions such as SO$_4$, Cl and NO$_3$ were measured with ion chromatograph (IC: model Dionex 120). Sample analysis is still in progress. The preliminary data show that the studied creek is severely polluted by Al, Fe, Mn, Pb and Zn. Toward upstream sites with relatively lower pH, less than 50% of Al and Fe occur in the sorbed form on particles or colloids, whereas more than 80% of Al and Fe occur in the sorbed form in downstream sites or tributaries with relatively higher pH. Less than 30% of Zn is present in particle or colloidal forms in the whole range of creek. Truly dissolved fraction of trace metals is negatively correlated with pH. The Kd values for Al, Fe and Zn consistently increase with increasing pH and decrease with increasing particle concentration.

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Experimental Studies on the Second Intermediate Hosts of Clonorchis sinensis II. Observations on the fate of encysted cercariae of Clonorchis sinensis in the fish hosts, Pseudorasbora parva, Acheilognathus signifer and Acanthorhodeus asmussi (간흡충(肝吸蟲)의 제이중간숙주(第二中間宿主)에 관한 실험적(實驗的) 연구(硏究) Ⅱ. 참붕어, 묵납자루 및 큰납지리 체내(體內)에 있어서 간흡충(肝吸蟲) 피낭유충(被囊幼蟲)의 운명(運命)에 관하여)

  • Rhee, Jae Ku
    • Korean Journal of Veterinary Research
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    • v.13 no.2
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    • pp.147-160
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    • 1973
  • The present experiments proposed to pass judgement upon the suitability as the second intermediate host of Clonorchis sinensis, of three kinds of fresh-water fish by having them infected with the cercaria of Clonorchis sinensis and then observing the penetrating ability of the cercaria, and maturity, the process of degeneration and extinction, and infectivity of the metacercaria of Clonorchis sinensis. The following is a brief summary of the leading facts gained through the experiments; 1. P. parva was easily subject to invasion of the cercaria, A. signifer was much less subject to the invasion, and finally A. asmussi was hard to invade. And the infectivity of the cercariae was in proportion to their penetrating ability mentioned in the above. 2. The examination of the cercariae after having three kinds of fresh-water fish infected with them disclosed that 24 hours after the infection, all of the cercariae formed their cysts in muscle and the metacercariae kept growing, and that 7 days later the metacercariae were found folding their bodies twice, and that in 15 days the characteristic structure of the metacercariae was complete and they made a vigorous rotary movement intermittently. 3. Then the metacercariae came to a state of maturity and beyond this stage some metacercariae in P. parva started the process of degeneration and extinction in 133 days; some in A. asmussi, in 140 days; and A. signifer, in 70 days. As more days elapsed, their degeneration and extinction increased in number, and in 269 days all of them in A. signifer became dead while those in A. asmussi were all dead in 460 days. However almost all of them in P. parva survived even after 770 days. The results shown above revealed that P. parva was the most suitable as the second intermediate host among three kinds of fresh-water fish and that A. signifer and A. asmussi were not quite recommendable as the second intermediate host. The ability of the cercaria to invade fresh-water fish, and life span of the metacercaria within fresh-water fish vary outstandingly according to species of fresh-water fish. An explanation as to the mechanism must wait as the subject to be further pursued.

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NATURAL ATTENUATION OF HAZARDOUS INORGANIC COMPONENTS: GEOCHEMISTRY PROSPECTIVE (유해 무기질의 자연정화 : 지화학적 고찰)

  • Lee, Suk-Young;Lee, Chae-Young;Yun, Jun-Ki
    • Proceedings of the KSEEG Conference
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    • 2002.06a
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    • pp.81-100
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    • 2002
  • While most of regulatory communities in abroad recognize ' 'natural attenuation " to include degradation, dispersion, dilution, sorption (including precipitation and transformation), and volatilization as governing Processes, regulators prefer "degradation" because this mechanism destroys the contaminant of concern. Unfortunately, true degradation only applies to organic contaminants and short- lived radionuclides, and leaves most metals and long-lived radionuclides. The natural attenuation Processes may reduce the potential risk Posed by site contaminants in three ways: (i)contaminants could be converted to a less toxic form througy destructive processes such as biodegradation or abiotic transformations; (ii) potential exposure levels may be reduced by lowering concentrations (dilution and dispersion); and (iii) contaminant mobility and bioavailability may be reduced by sorption to geomedia. In this review, authors will focus will focul on "sorption" among the natural attenuation processes of hazardous inorganic contaminants including radionuclides. Note though that sorption and transformation processes of inorganic contaminants in the natural setting could be influenced by biotic activities but our discussion would limit only to geochemical reactions involved in the natural attenuation. All of the geochemical reactions have been studied in-depth by numerous researchers for many years to understand "retardation" process of contaminants in the geomedia. The most common approach for estimating retardation is the determination of distrubution coefficiendts ($K_{d}$) of contaminants using parametric or mechanistic models. As typocally used in fate and contaminant transport calculations such as predictive models of the natural attenuation, the $K_{d}$ is defined as the ratio of the contaminant concentration in the surrounding aqueous solution when the system is at equilibrium. Unfortunately, generic or default $K_{d}$ values can result in significant error when used to predict contaminant migration rate and to select a site remediation alternative. Thus, to input the best $K_{d}$ value in the contaminant transport model, it is essential that important geochemical processes affecting the transport should be identified and understood. Precipitation/dissolution and adsorption/desorption are considered the most important geochemical processes affecting the interaction of inorganic and radionuclide contaminants with geomedia at the near and far field, respectively. Most of contaminants to be discussed in this presentation are relatively immobile, i.e., have very high $K_{d}$ values under natural geochemical environments. Unfortunately, the obvious containment in a source area may not be good enough to qualify as monitored natural attenuation site unless owner demonstrate the efficacy if institutional controls that were put in place to protect potential receptors. In this view, natural attenuation as a remedial alternative for some of sites contaminated by hazardous-inorganic components is regulatory and public acceptance issues rather than scientific issue.

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The Attitudes of Nursing Students Towards Poverty - Based on the Factors Causing Poverty and the Factors Caused by Poverty (간호학생들의 빈곤에 대한 태도 -발생요인과 결과요인을 중심으로-)

  • Im, Mi-Lim;Yang, Soon-Ok
    • Research in Community and Public Health Nursing
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    • v.10 no.2
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    • pp.525-536
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    • 1999
  • The result of this study may be abstracted as follows. 1) The study was done on 48.3% seniors(138), 34.3% sophomores. and 17.5% juniors mostly ranging in age from 21 to 22, the largest number of students at 38.1%(109) were Christian. followed by 31.5%(90) were Confucian. 20.6%(59) who were Catholic. and 9.1%(26)who were Buddhist. 2) Concerning the economic views. 46.8%(134) preferred Capitalism 40.9%(117) preferred a combination of Capitalism and Socialism, 8.7%(25) were for more or less Socialistic in view point, and 3.5%(10) for Socialism. 3) Among the internal factors causing poverty, 'lack of ability(knowledge)', scored the highest at 3.528, and 'beauty or appearance' scored the lowest at 1.570. Social factors scored high in 'bad economic/tax policies' at 3.374 and 'low average in wages' at 3.364. Among family matters, 'not much money at home' scored the highest at 3.273, and 'low expectation of success in poor families' scored the lowest at 2.315. On concerns of fate and destiny. 'bad luck' turned out to be the most likely cause of poverty with the score of 2.619. and 'destined poverty' was the least likely cause with the score of 1.969. 4) The high scoring economical consequences of poverty was 'not able to do as one desires' at 3.965, and 'no money to buy things wanted' at 3.804. 'Gets tired of life' (4.028) scored high in psychological consequences showing that poverty affects these matters greatly. 'Looked down on by others' (3.538) scored high in social circumstances, and 'feels like a burden to society' scored low at 2.080. Among family matters. 'hard life' scored high at 4.210, and 'low morality' scored low at 1.972. 5) Significant differences(p= .00) were found between economic views and what significance certain internal factors caused poverty these differences were also related to a reliance on religion, economic views, and economic status. Significant differences were also found between economic status and internal factors causing poverty(p= .04), economic point of view and social factors(p= .01). and economical point of view and luck related matters, (p = .01) When the consequences of poverty were considered. psychological factors such as 'hate towards oneself', 'tired of life', and 'afraid of the future' showed significant differences in relation to economic status. (p = .02) Family matters also showed significant differences in relation to economic status(p= .03), With personal characteristics and wealth causing factors, significant differences were found between a person's economic point of view and ecinomic status when considering and internal or social factors.

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