• Journal of Nutrition and Health
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• 제14권1호
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• pp.34-40
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• 1981

식물성(植物性), 동물성(動物性) 고지방식(高脂肪食)과 저지방식(低脂肪食)을 장기간(長期間) 섭취 했을 때 혈청(血淸) 지질성분(脂質成分)과 지단백분획(脂蛋白分劃)의 함대비(含臺比)에 어떠한 영향을 미치는 가를 알아보기 위하여 흰쥐를 사용하여 대조군(對照群)(12%-fat 이저(館低)), 저지방식이군(低脂肪食餌群)(3%-fat 식이(食餌)), 식물성고지방식이군(植物性高脂肪食餌群)(45%-corn oil식이(食領)) 및 동물성고지방식이군(動物性高脂肪食餌群(45%-butter fat 식이(食餌))를 각각 12주간(週間) 사육(飼育)하면서 4, 8 및 12주(週)에 쥐를 희생하여 혈청(血淸)중의 총(總) cholesterol, TG, 인지질(燐指質) 및 지단백분획(脂蛋白分劃)의 함대비(含臺比)를 측정(測定)하였다. 혈청(血淸) 총(總) cholesterol 함양(含量)은 45%-butter fat식이군(食餌群) 이 전실험(全實驗)기간 동안 대조군(對照群)보다 증가된 치(値)를 보였으며 45%-corn oil 식이군(食餌群)은 8주 및 12주에 증가되었다. 또 45%-butter fat 식이군(食餌群)과 비교했을 때 45%-corn oil 식이군(食餌群)이 4.8주에는 유의적(有意的)으로 낮았고 12주에는 다소 낮았다. 또한 저지방식이군(低脂肪食餌群)은 대조군과 전실험(全實驗) 기간 동안 차이가 없었다. 혈청(血淸) TG 함양(含量)도 45%-butter fat 식이군(食餌群)이 실험전(全( 기간 동안 대조군(對照群)보다 증가된 치(値)를 나타내었으며, 45 %-corn oil식이군(食餌群)과 저지방식이군(低脂肪食餌群)은 12주(週)째에만 증가된 치(値)를 나타내었다. 혈청(血淸) 인지질(燐脂質)의 함양(含量)은 저지방식이군(低脂肪食餌群)에서만 식이투여(食餌投與) 12주(週)에 대조군(對照群)에 비해 낮은 치(値)를 보였다. 혈청(血淸) 지단백분획(脂蛋白分劃)의 함양비(含量比)는45%-butter fat 식이군(食餌群)이 4, 8 및 12주에, 45%-corn oil 식이군(食餌群)은 8, 12주(週)에, 저지방식이군(低脂肪食餌群)은 12주에 대조군(對照群)에 비(比)해 ${\alpha}$-lipoprotein의 함양비(含量比)는 감소(減少)되고 ${\beta}$-lipoprotein의 함양비(含量比)는 증가되었다. 그 중에서도 특히 저지방식이군(低脂肪食餌群)이 가장 헌저한 변동을 보였다.

• 생약학회지
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• 제45권1호
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• pp.69-76
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• 2014

The purpose of this study was to investigate anti-obesity and anti-hyperlipidemic effects of extracts from Geranium thunbergii (GT) in high-fat-diet-induced obese rats. Animals were randomly divided into four groups [normal diet, high fat diet, MeOH extract of GT (GTM), and BuOH fraction of GT (GTB)] and GT samples were treated with dose of 100 mg/kg for 8 weeks. It was observed that GTB-treated group significantly reduced body weight gain, food intake, epididymal fat weight, and triglyceride level in serum and liver compared to control group. The rats fed GTB also decreased contents of thiobarbituric acid-reactive substances (TBARS), hydroxyl radical, and xanthin oxidase (XO) increased by high fat diet. Furthermore, the anti-oxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase were increased by GTB treatment. The experimental results indicate that GTB has anti-obesity and anti-hyperlipidemic effects, as well as radical scavenging activity.

• 생약학회지
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• 제47권1호
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• pp.49-54
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• 2016

In our search for natural products affecting blood circulation, the n-butanol fraction from whole plant of Glehnia littoralis Fr. Schm. (GLB) improved blood lipid parameters, and ameliorated obesity in high-fat-diet (HFD)-fed C57BL/6 mouse model. Hyperlipidemia was induced by high-fat-diet for 4 weeks, and then GLB was orally administrated with 400 mg/kg/day for 4 weeks. GLB-treated group showed that the gain in body weight was significantly attenuated, the levels of total cholesterol and triglyceride significantly lowered on blood chemical analysis, and significantly prolonged the mice bleeding time when compared with those of HFD control group. Concomitantly, phytochemical composition of GLB was investigated by HPLC-hyphenated spectroscopy, and two major phenolic compounds, rutin and chlorogenic acid were identified in the GLB. Taken together, these results indicate that GLB has cardiovascular protective effects and could be a natural medicine candidate for the prevention of cardiovascular disease.

• 한국축산식품학회지
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• 제40권2호
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• pp.209-220
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• 2020

Milk fat globule membrane (MFGM) is a lipid carrier in mammals including humans that consists mainly of polar lipids, like phospholipids and glycolipids. In this study, a process to enrich polar lipids in commercial butter and whey powder, including polar lipids of MFGM, was developed. WPC (whey protein concentrate) 60 was selected as the most suitable raw material based on the yield, phospholipid, protein, and lactose content of the polar lipid fraction obtained by ethanol extraction of two WPC (WPC60 and WPC70) and two buttermilk (A and B). After fractionation under optimum conditions, the polar-lipid enriched fraction from WPC60 contained 38.56% phospholipids. The content of glycolipids, cerebroside, lactosylceramide, ganglioside GM3, ganglioside GD3, was 0.97%, 0.55%, 0.09%, and 0.14%, respectively. Rancimat results showed that the oxidation stability of fish oil increased with an increase in the polar-lipid fraction by more than 30 times. In addition, the secretion of IL-6 and TNF-α decreased in a concentration-dependent manner after treatment of RAW 264.7 cells with 0.1 to 100 ppm of the polar lipid fraction. In this study, polar lipid concentrates with antioxidant and anti-inflammatory activity, were prepared from milk processing by-products. The MFGM polar lipid concentrates made from by-products are not only additives for infants, but are also likely to be used as antioxidants in cooking oils and as active ingredients for functional foods.

• Journal of Ginseng Research
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• 제46권3호
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• pp.454-463
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• 2022

Background: Gintonin-enriched fraction (GEF), a non-saponin fraction of ginseng, is a novel glycolipoprotein rich in hydrophobic amino acids. GEF has recently been shown to regulate lipid metabolism and browning in adipocytes; however, the mechanisms underlying its effects on energy metabolism and whether it affects sarcopenic obesity are unclear. We aimed to evaluate the effects of GEF on skeletal muscle atrophy in high-fat diet (HFD)-induced obese mice. Methods: To examine the effect of GEF on sarcopenic obesity, 4-week-old male ICR mice were used. The mice were divided into four groups: chow diet (CD), HFD, HFD supplemented with 50 mg/kg/day GEF, or 150 mg/kg/day GEF for 6 weeks. We analyzed body mass gain and grip strength, histological staining, western blot analysis, and immunofluorescence to quantify changes in sarcopenic obesity-related factors. Results: GEF inhibited body mass gain while HFD-fed mice gained 22.7 ± 2.0 g, whereas GEF-treated mice gained 14.3 ± 1.2 g for GEF50 and 11.8 ± 1.6 g for GEF150 by downregulating adipogenesis and inducing lipolysis and browning in white adipose tissue (WAT). GEF also enhanced mitochondrial biogenesis threefold in skeletal muscle. Furthermore, GEF-treated skeletal muscle exhibited decreased expression of muscle-specific atrophic genes, and promoted myogenic differentiation and increased muscle mass and strength in a dose-dependent manner (p < 0.05). Conclusion: These findings indicate that GEF may have potential uses in preventing sarcopenic obesity by promoting energy expenditure and attenuating skeletal muscle atrophy.

• 보건교육건강증진학회지
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• 제15권1호
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• pp.195-204
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• 1998

The purpose of this study was to develop cardiovascular health related percent body-fat standards that may be applied to epidemiologic investigations of the prevalence and incidence of obesity in adolescents, pediatric health screenings, and youth fitness tests. The subjects included 102 males and 80 females aged 19～22years. All subject were Honam University Students Total body fat was derived from body density which was estimated from age and the triceps and subscapular skinfold thickness measured with Lang calipers to the nearst l.0mm. Serum total cholesterol and lipoprotein cholesterol fraction(HDL-CLDL-C) were measured from blood obtained from fore arm vein after blood pressure measurement. In analyses to determine critical fat levels associated with elevated CDD(Chronic Degenerative Disease) risk factors;male and female were grouped by level of percent fat as follows: male, 〈 10%, 10-14.9%, 15-19.9%, 20-24.9%, and $\geq$ 25%;female, 〈 20%, 20-24.9%, 25-29.9%, 30-34.9%, and $\geq$ 35%. As the results of the data, the conclusions were as follows: 1. A dose respones effect was observed between blood pressure and percent body fat in males and females; in contrast, total cholesterol and lipoprotein ratios were relatively independant of percent fat among the lower four fatness group in males and the lower three fatness groups in females. 2. The percentage of subjects in the uppermost quintile for S-Bp, D-Bp, TC, LDL/H was significantly(P〈.05) greater than expected by change alone(20%) in males with $\geq$ 25% fat and in females with $\geq$ 30% fat females with $\geq$35% had even greater representation in the uppermost quintile of all CDD risk factors compared to females with 30-34.9% fat.

• 대한한의학방제학회지
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• 제19권1호
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• pp.219-231
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• 2011

Objectives : This study was designed to determine the effects of the GGEx18 ethyl acetate fraction(EF) on body weight gain, feeding efficiency ratio, and obesity-related factors in plasma as well as histology of liver and adipose tissues using high fat diet-fed male C57BL/6N obese mice. Methods : 8 weeks old, high fat diet-fed obese male mice were divided into 5 groups: C57BL/6N normal, control, EF(1), EF(2) and EF(3). After mice were treated with EF for 9 weeks, we measured body weight gain, food intake, feeding efficiency ratio, fat weight, plasma leptin and lipid levels. We also analysed histology of liver and adipose tissues on high fat diet-fed male C57BL/6N obese mice. Results : Compared with control, EF-treated mice had significantly lower body weight gain and feeding efficiency ratio. Consistent with the effects on body weight gain, EF significantly decreased the adipose tissue weight compared with control. Consistent with the effects on feeding efficiency ratio, EF significantly decreased plasma leptin concentrations compared with control. EF reduced the size of adipocytes as well as hepatic lipid accumulation compared with control. EF seems to be safe since not only the plasma levels of ALT and AST are within the normal range, but also EF did not show any toxic effects on organs. EF(3) was most effective among EF(1), EF(2), and EF(3) at doses of 25, 50, and 100 mg/kg, respectively. Conclusions : These results demonstrate that EF effectively reduces body weight gain, feeding efficiency ratio in high fat diet-fed obese mice, leading to the modulation of obesity. In addition, EF decreases the size of adipocytes and improves plasma lipids and controls hepatic lipid accumulation, suggesting that EF may act as a therapeutic agent for obesity.

• 한국축산식품학회지
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• 제23권2호
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• pp.155-160
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• 2003

인유 초유로부터 지방을 제거하여 skim milk을 얻었다. 이것을 ultrafiltration을 한 후 gel filtration을 거쳐 EGF fraction 을 획득하였으며, 이를 SDS-PAGE로 확인하였다. hish performance liquid chromatography(HPLC)에 의해 EGF fraction을 분석한 결과, standard EGF 분석과 비교했을 때 31.545분대와 유사한 31.185분대에 peak가 나타난 것을 인하였다. 분리한 fraction 중 EGF 함유량을 측정하기 위해 immunoassay를 실시한 결과 건조중량 10 mg/mL 중에 약 10ng의 EGF가 포함되어 있는 것으로 측정되었다. SDS- PAGE후 실시한 western blot을 통하여 분리한 분획의 분리도를 확인하였다. 세포성장에 미치는 영향을 살펴본 결과, fibroblast cell인 BALB/3T3의 경우에는 건조중량 1 mg/mL 농도의 fraction 처리후 약 95%의 성장률을 보였으며, epithelial cell 인 IEC-6의 경우에는 약 71%의 성장률을 보였다.

• 통합자연과학논문집
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• 제5권1호
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• pp.46-52
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• 2012

Mulberry extracts can be incorporated into skin-whitening products. The compound attributed to lighten the skin is arbutin, a form of hydroquinone that inhibits melanin release by suppressing the tyrosinase enzyme. For the cosmetic applications, the physiological effects of mulberry (Morus alba) extracts were investigated. The water soluble fraction of mulberry contains higher amount of protein (16.28~4.47%) in contrast to fat (1.55~1.41%). In addition, the fraction abundantly contains succinic acid (972.4-275.8 mg/g) and phosphoric acid (1,628.4-121.9 mg/g) in different parts of mulberry. The free radical scavenging ability in water soluble fraction was found to display remarkable effects in comparison with methanol and ethyl acetate fraction. The ethyl acetate-soluble of root and leaf showed remarkable tyrosinase inhibition activity by IC 50 (${\mu}g/ml$). The anticancer activity of methanol fraction obtained from mulberry using human cancer cell lines showed growth inhibition effect (270.14 mg/ml in Calu-6 cells, 295.29 mg/ml in HCT-116, and 332.29 mg/ml in MCF-7 cells, respectively). Based on the results, Morus alba extracts include cosmetic ingredients with antioxidizing and whitening properties.

• Journal of Applied Biological Chemistry
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• 제44권2호
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• pp.77-83
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• 2001

For defining the possibilities of the commercial mass liquid culture of Cordyceps sinensis, the pharmacological activities of mycelia were analyzed. The mycelium of C. sinensis consists of carbohydrate (5.1%) and fat (1.3%), and contains a low content of protein (0.7%) and ash (0.5%), and 92.4% moisture. The molecular sugar ratio of carbohydrate was composed mainly of glucose, mannose (1.0 : 0.9), in addition a small amount of galactose and arabinose (0.2 : 0.1). The cellular materials of mycelia were fractionated into ethylacetate (EA), MeOH (M) and hot-water extract fraction (HW). HW fraction showed the most potent intestinal immune system modulating activity, anti-coagulant activity, and anti-complementary activity, and M fraction had the inhibition activity of radical generation as effective as genistine. These results reveal that the mycelium of liquid cultured C. sinensis showed pharmacological activities and could be used for commercial purpose.